FastQCFastQC Report
Wed 25 May 2016
SRR1032981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3993668
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83070.20800427076061406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64180.16070439505737583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59750.050.165561
GTATCAA111750.042.7979741
ATCAACG133750.035.140193
TCAACGC134250.035.0093154
CAACGCA137050.034.3944555
TATCAAC142800.033.2092672
AACGCAG142700.033.065216
ACGCAGA164700.028.4760387
CGCAGAG168250.027.7071028
GTACATG122100.025.2797411
GCAGAGT184900.024.8973039
TACATGG123000.023.9439742
TACACCG6100.023.1361545
ACATGGG125650.022.2785473
GAGTACT114950.020.98404112-13
ACACCGT7400.020.97866
AGAGTAC167200.020.93040810-11
GTGGTAT30850.020.1021121
CAGAGTA182650.020.0086410-11
AGTACTT122400.019.09278912-13
Produced by FastQC (version 0.11.5)