##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032981_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3993668
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.853036857345174	34.0	31.0	34.0	31.0	34.0
2	32.91111579630555	34.0	31.0	34.0	31.0	34.0
3	32.99684375366205	34.0	33.0	34.0	31.0	34.0
4	36.396798131442075	37.0	37.0	37.0	35.0	37.0
5	36.37118859154041	37.0	37.0	37.0	35.0	37.0
6	36.42978760377678	37.0	37.0	37.0	35.0	37.0
7	36.40375965152837	37.0	37.0	37.0	35.0	37.0
8	36.33767854513695	37.0	37.0	37.0	35.0	37.0
9	38.18162200763809	39.0	39.0	39.0	37.0	39.0
10-11	38.200352658258026	39.0	39.0	39.0	37.0	39.0
12-13	38.19028697428028	39.0	39.0	39.0	37.0	39.0
14-15	39.758140010636836	41.0	40.0	41.0	37.0	41.0
16-17	39.770950289307976	41.0	40.0	41.0	37.0	41.0
18-19	39.79649672932251	41.0	40.0	41.0	37.5	41.0
20-21	39.80918256099405	41.0	40.0	41.0	37.5	41.0
22-23	39.7731512734659	41.0	40.0	41.0	37.0	41.0
24-25	39.75618842127087	41.0	40.0	41.0	37.0	41.0
26-27	39.68650849294433	41.0	40.0	41.0	37.0	41.0
28-29	39.62907833099797	41.0	40.0	41.0	37.0	41.0
30-31	39.52272810358798	41.0	40.0	41.0	36.5	41.0
32-33	39.404886560425155	41.0	39.5	41.0	36.0	41.0
34-35	39.317455532107324	41.0	39.0	41.0	35.0	41.0
36-37	39.23323145539389	41.0	39.0	41.0	35.0	41.0
38-39	39.137933849283414	41.0	39.0	41.0	35.0	41.0
40-41	39.039681941513415	41.0	39.0	41.0	35.0	41.0
42-43	38.909122641140925	41.0	39.0	41.0	35.0	41.0
44-45	38.7477276528745	41.0	39.0	41.0	35.0	41.0
46-47	38.5384131830688	41.0	39.0	41.0	35.0	41.0
48-49	38.342462743523	41.0	39.0	41.0	35.0	41.0
50-51	38.19540106989364	41.0	38.0	41.0	34.0	41.0
52-53	38.0623043778301	40.0	38.0	41.0	34.0	41.0
54-55	37.91853942290646	40.0	38.0	41.0	34.0	41.0
56-57	37.720924849036024	40.0	37.0	41.0	33.5	41.0
58-59	37.56153591134766	40.0	37.0	41.0	33.0	41.0
60-61	37.38307653515515	40.0	36.5	41.0	33.0	41.0
62-63	37.154469024465726	39.5	36.0	41.0	33.0	41.0
64-65	36.87205934494304	39.0	35.5	41.0	32.5	41.0
66-67	36.60259828809005	39.0	35.0	41.0	32.0	41.0
68-69	36.31289982041572	38.0	35.0	40.5	32.0	41.0
70-71	35.98647183491467	37.0	35.0	40.0	32.0	41.0
72-73	35.64355649993941	37.0	35.0	39.5	31.0	41.0
74-75	35.294031827382746	36.0	35.0	39.0	31.0	41.0
76-77	34.93575079350612	36.0	35.0	39.0	31.0	40.5
78-79	34.58365179579274	35.5	35.0	37.5	31.0	39.5
80-81	34.241263169597474	35.0	35.0	37.0	31.0	39.0
82-83	33.92368206370685	35.0	34.0	37.0	30.0	39.0
84-85	33.6369773351215	35.0	34.0	36.0	30.0	37.5
86-87	33.4845236258998	35.0	34.0	36.0	30.0	37.0
88-89	33.28512597942543	35.0	34.0	36.0	30.0	37.0
90-91	33.10434067128264	35.0	34.0	35.0	30.0	36.5
92-93	32.94191743029215	35.0	34.0	35.0	30.0	36.0
94-95	32.84333587569122	35.0	34.0	35.0	30.0	36.0
96-97	32.7787248464319	35.0	34.0	35.0	30.0	36.0
98-99	32.67090854823185	35.0	34.0	35.0	30.0	36.0
100	32.59051428411175	35.0	34.0	35.0	30.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18708949258305552
1101	2	0.2056145731021033
1101	3	0.19118175963706818
1101	4	0.12359610755360251
1101	5	0.14191387501463026
1101	6	0.12317504224602516
1101	7	0.12341832576145606
1101	8	0.053121061785930124
1101	9	0.1402544934183112
1101	10-11	0.148041683926877
1101	12-13	0.18811615517774527
1101	14-15	0.2594474812292731
1101	16-17	0.24118598725671347
1101	18-19	0.25373570004848744
1101	20-21	0.25749479430316313
1101	22-23	0.24750487166024726
1101	24-25	0.24107092337665392
1101	26-27	0.2505467402303978
1101	28-29	0.26411829969422485
1101	30-31	0.23782032583640955
1101	32-33	0.27901425483093334
1101	34-35	0.24141464496982223
1101	36-37	0.24283540739708087
1101	38-39	0.2122285232809915
1101	40-41	0.2089991648234104
1101	42-43	0.20356772567354398
1101	44-45	0.2148698644880369
1101	46-47	0.23979662794815226
1101	48-49	0.20707684629287115
1101	50-51	0.15898775622456895
1101	52-53	0.18994229690510167
1101	54-55	0.2710021901459285
1101	56-57	0.22488197053363734
1101	58-59	0.23899663777429936
1101	60-61	0.26584526454771407
1101	62-63	0.2632222926619079
1101	64-65	0.2964619273631328
1101	66-67	0.31388089984282175
1101	68-69	0.2676380300595511
1101	70-71	0.2978870415046089
1101	72-73	0.26335285611206416
1101	74-75	0.284776504948816
1101	76-77	0.284175043896127
1101	78-79	0.2565833264483999
1101	80-81	0.2592799460002908
1101	82-83	0.21970327220840602
1101	84-85	0.26453823513676156
1101	86-87	0.2931829543403808
1101	88-89	0.2667123016451356
1101	90-91	0.21139194777292403
1101	92-93	0.27406978335537246
1101	94-95	0.29967547435713016
1101	96-97	0.25168951348365454
1101	98-99	0.24715318892500449
1101	100	0.25299677534630405
1102	1	0.19999002285747025
1102	2	0.2328123043222945
1102	3	0.1951835024910693
1102	4	0.12433298349633048
1102	5	0.10672254903868605
1102	6	0.14672318261910533
1102	7	0.15239813372350852
1102	8	0.05518503066694791
1102	9	0.1257424200615489
1102	10-11	0.15732400154752213
1102	12-13	0.19797283016360723
1102	14-15	0.2420145073524722
1102	16-17	0.24342876067972696
1102	18-19	0.26262075950931063
1102	20-21	0.2541199331815207
1102	22-23	0.2585995357473081
1102	24-25	0.20692812360618973
1102	26-27	0.25717359297202336
1102	28-29	0.27680941463476927
1102	30-31	0.3008488842557
1102	32-33	0.29729324619254527
1102	34-35	0.3402145996078687
1102	36-37	0.3427030732773062
1102	38-39	0.32894380625175756
1102	40-41	0.27927096989661493
1102	42-43	0.25670814564679034
1102	44-45	0.24005853866557203
1102	46-47	0.27006046909945525
1102	48-49	0.29393304975317136
1102	50-51	0.2667084971251157
1102	52-53	0.26569735599927924
1102	54-55	0.24397934119355824
1102	56-57	0.24704247851931171
1102	58-59	0.21526056930844817
1102	60-61	0.21226401506464043
1102	62-63	0.24942236786725402
1102	64-65	0.24251573584871267
1102	66-67	0.2928860746867201
1102	68-69	0.2475298946660658
1102	70-71	0.23612759457937216
1102	72-73	0.2107497876999247
1102	74-75	0.19865879750101811
1102	76-77	0.19726094425842433
1102	78-79	0.22116340010189361
1102	80-81	0.21821961886557517
1102	82-83	0.25779571679989033
1102	84-85	0.20036010931872994
1102	86-87	0.19186916302754042
1102	88-89	0.21066581758022807
1102	90-91	0.1503301540487314
1102	92-93	0.15985094262613586
1102	94-95	0.16940574979457068
1102	96-97	0.18297817666177707
1102	98-99	0.23704293038745305
1102	100	0.27300293161141553
1103	1	0.1491102748008828
1103	2	0.16022693945934918
1103	3	0.1560066163468079
1103	4	0.1196451103695253
1103	5	0.11655968271721662
1103	6	0.11593483415023087
1103	7	0.14158659959475983
1103	8	0.07088294752811208
1103	9	0.06585210480798764
1103	10-11	0.12035435550062346
1103	12-13	0.18740284881238267
1103	14-15	0.1937959672759746
1103	16-17	0.18736078719091154
1103	18-19	0.19191250606299803
1103	20-21	0.19821080013455372
1103	22-23	0.20945483043416857
1103	24-25	0.1420000702352766
1103	26-27	0.21079052970675605
1103	28-29	0.20203073155273188
1103	30-31	0.20552832236214158
1103	32-33	0.25641798917011016
1103	34-35	0.2468382525842543
1103	36-37	0.2201575905009605
1103	38-39	0.25762887010731106
1103	40-41	0.23676827806050227
1103	42-43	0.20872103632336092
1103	44-45	0.21681579908888438
1103	46-47	0.22939223859638957
1103	48-49	0.20138741301723684
1103	50-51	0.20747834096094664
1103	52-53	0.27088538488734315
1103	54-55	0.28910324427238976
1103	56-57	0.3004793316638015
1103	58-59	0.25844061784830075
1103	60-61	0.24152364302619134
1103	62-63	0.21649285089746684
1103	64-65	0.28585179131871996
1103	66-67	0.2634256277256313
1103	68-69	0.22294611787996388
1103	70-71	0.2528227765284967
1103	72-73	0.2205196213809799
1103	74-75	0.24168940696154095
1103	76-77	0.2654849122295815
1103	78-79	0.2321464353923801
1103	80-81	0.22795569608646815
1103	82-83	0.20887749587834747
1103	84-85	0.2211743000694284
1103	86-87	0.2135217456669949
1103	88-89	0.24057287839655572
1103	90-91	0.2474154040971328
1103	92-93	0.32110072110448584
1103	94-95	0.26860008250911704
1103	96-97	0.25135716884226156
1103	98-99	0.2858950141978198
1103	100	0.29697768354811416
1104	1	0.08846583898500882
1104	2	0.13184141689173856
1104	3	0.14592518255511067
1104	4	0.10948865047180334
1104	5	0.0505459275353104
1104	6	0.10432947359276312
1104	7	0.09458322851338608
1104	8	0.09403879740515464
1104	9	0.11612412678248063
1104	10-11	0.09305293047958685
1104	12-13	0.12493844402813181
1104	14-15	0.19994195214188437
1104	16-17	0.18335645236312104
1104	18-19	0.13874651063155596
1104	20-21	0.08377603669301692
1104	22-23	0.13115327710941216
1104	24-25	0.11985097945731127
1104	26-27	0.13415039372107174
1104	28-29	0.17205324000949673
1104	30-31	0.14368904659248471
1104	32-33	0.13980264085991934
1104	34-35	0.150995370686843
1104	36-37	0.07258711455172318
1104	38-39	0.1063479923025028
1104	40-41	0.12268199072657637
1104	42-43	0.13453318532788927
1104	44-45	0.12640210745261982
1104	46-47	0.14583371757748864
1104	48-49	0.1322113746493656
1104	50-51	0.11014251454854929
1104	52-53	0.08280057732695667
1104	54-55	0.029456593170905876
1104	56-57	0.058725594404414494
1104	58-59	0.0691044898315809
1104	60-61	0.1225793606435559
1104	62-63	0.07859035271249581
1104	64-65	0.067097289674237
1104	66-67	0.06292232785813212
1104	68-69	0.057764911866392765
1104	70-71	0.04898915812095339
1104	72-73	0.09948624785664606
1104	74-75	0.09866285384458706
1104	76-77	0.07192379263884163
1104	78-79	0.07511109275305472
1104	80-81	0.06976054234169737
1104	82-83	0.044611242758335834
1104	84-85	0.07752308665575214
1104	86-87	0.09581609711051442
1104	88-89	0.05799694313412118
1104	90-91	0.057693599398362494
1104	92-93	0.06644721203569759
1104	94-95	0.03878465172958556
1104	96-97	0.06865807014922609
1104	98-99	0.02583370402639673
1104	100	0.02052591902221934
1105	1	0.055483657201492065
1105	2	0.09699629563297663
1105	3	0.06701104843196504
1105	4	0.0705547562949036
1105	5	0.05682926798849053
1105	6	0.04448269628807111
1105	7	0.05322585248480749
1105	8	1.0619664949729213E-4
1105	9	0.06085497758078873
1105	10-11	0.06565973167921157
1105	12-13	0.07506592515743193
1105	14-15	0.10494937727141007
1105	16-17	0.12519167618184213
1105	18-19	0.13253868706964766
1105	20-21	0.12549264154693418
1105	22-23	0.1255234988484233
1105	24-25	0.1166084654581141
1105	26-27	0.0769294739624371
1105	28-29	0.10691639077645476
1105	30-31	0.11557067320717351
1105	32-33	0.15746747487565216
1105	34-35	0.1672034377937166
1105	36-37	0.1703273792904838
1105	38-39	0.17142494263841712
1105	40-41	0.12710442623249207
1105	42-43	0.12065876668678044
1105	44-45	0.1008755431893249
1105	46-47	0.14160893048037337
1105	48-49	0.15154004863438075
1105	50-51	0.17579768970255572
1105	52-53	0.1342192177799646
1105	54-55	0.15625183223183114
1105	56-57	0.11268667675653887
1105	58-59	0.08248899114453678
1105	60-61	0.1615672446106018
1105	62-63	0.11268341668947812
1105	64-65	0.07359395636942878
1105	66-67	0.09600462038579138
1105	68-69	0.10313706981781223
1105	70-71	0.06126943433565657
1105	72-73	0.09008128326132692
1105	74-75	3.515748942319874E-4
1105	76-77	0.023179628966573773
1105	78-79	0.028870296074991586
1105	80-81	-0.007980267530889762
1105	82-83	0.014917830565892132
1105	84-85	0.033182404467744675
1105	86-87	-0.009500211252834845
1105	88-89	0.0019087318653845387
1105	90-91	0.03383355290222312
1105	92-93	0.0021575492270002883
1105	94-95	-0.0491141673645501
1105	96-97	-0.0182444884671753
1105	98-99	0.021003973971758683
1105	100	0.03106067495335907
1106	1	0.06264966223488244
1106	2	0.11040618779210831
1106	3	0.06742252245443581
1106	4	0.05644223756763722
1106	5	0.05852584704432928
1106	6	0.04524611929885225
1106	7	0.05142646271465168
1106	8	0.039430110155805664
1106	9	0.04992086451188982
1106	10-11	0.09516525086349503
1106	12-13	0.11370859487486484
1106	14-15	0.1393281763266785
1106	16-17	0.10676434376561872
1106	18-19	0.11527183275850206
1106	20-21	0.10191905207641838
1106	22-23	0.10810338202216485
1106	24-25	0.12101317350689555
1106	26-27	0.14101172738836
1106	28-29	0.10903634886158642
1106	30-31	0.10317730103655265
1106	32-33	0.1185578960609277
1106	34-35	0.0905624424056839
1106	36-37	0.14906255206680896
1106	38-39	0.1553377531423763
1106	40-41	0.195138881865077
1106	42-43	0.11396274936233652
1106	44-45	0.10260757720482871
1106	46-47	0.046291406610833974
1106	48-49	0.09417632224242567
1106	50-51	0.11043400099050871
1106	52-53	0.06973375448680486
1106	54-55	0.039166407913114654
1106	56-57	0.0762740286461181
1106	58-59	0.006584488023698043
1106	60-61	0.034073001201520015
1106	62-63	0.051180475459304375
1106	64-65	0.009091502435445875
1106	66-67	0.0684354852371527
1106	68-69	0.05954434449359525
1106	70-71	0.09384924815984164
1106	72-73	0.03872328606720998
1106	74-75	0.04071284215100235
1106	76-77	0.019649910024142514
1106	78-79	0.017229597734221613
1106	80-81	0.036830033698151965
1106	82-83	0.07047424978024708
1106	84-85	0.05286785338244471
1106	86-87	0.030379538931576633
1106	88-89	0.0845608888330176
1106	90-91	0.05875804279579455
1106	92-93	0.06485234923560057
1106	94-95	0.06207359747850916
1106	96-97	-0.02641606203862068
1106	98-99	-0.0037304981429002737
1106	100	0.019394577338310626
1107	1	0.03608897932616628
1107	2	0.061759780596638336
1107	3	0.04998899606798801
1107	4	0.0278255346091143
1107	5	0.06386601319615437
1107	6	0.03537735632240668
1107	7	0.05603834867383739
1107	8	0.028281915020251347
1107	9	0.07149774771254158
1107	10-11	0.07146988076211613
1107	12-13	0.03772547214088462
1107	14-15	0.08057274476458076
1107	16-17	0.08391824520636249
1107	18-19	0.08334899536642837
1107	20-21	0.093101453504282
1107	22-23	0.1060614655991543
1107	24-25	0.1001438592674262
1107	26-27	0.10674574453659602
1107	28-29	0.12530960207743647
1107	30-31	0.1693468537272551
1107	32-33	0.1526021077103863
1107	34-35	0.1736549590879477
1107	36-37	0.1737522278566388
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2216	12-13	-0.2698339271054522
2216	14-15	-0.40569261320030137
2216	16-17	-0.3904666656650946
2216	18-19	-0.37122147936344874
2216	20-21	-0.3430747351803305
2216	22-23	-0.3388323974500125
2216	24-25	-0.36461662804251205
2216	26-27	-0.36907446139235844
2216	28-29	-0.3224682247074284
2216	30-31	-0.35092520822998097
2216	32-33	-0.4191644386468383
2216	34-35	-0.40044909246373095
2216	36-37	-0.45466823063004824
2216	38-39	-0.3645272962468056
2216	40-41	-0.3099905975790236
2216	42-43	-0.32360033677095856
2216	44-45	-0.34252983384451596
2216	46-47	-0.2980066902934624
2216	48-49	-0.4006817556020863
2216	50-51	-0.34750075006551384
2216	52-53	-0.33547932456615825
2216	54-55	-0.30692180760171794
2216	56-57	-0.32071203841814366
2216	58-59	-0.36758794701447783
2216	60-61	-0.4059906166367142
2216	62-63	-0.3485773698855468
2216	64-65	-0.3412712490637091
2216	66-67	-0.36904020728019304
2216	68-69	-0.36133610267160776
2216	70-71	-0.3581836461082091
2216	72-73	-0.3681553031677467
2216	74-75	-0.37586247219858393
2216	76-77	-0.3462169358296592
2216	78-79	-0.38108795277140217
2216	80-81	-0.40192560359803764
2216	82-83	-0.3929006405471043
2216	84-85	-0.33463285601076365
2216	86-87	-0.2806808121623021
2216	88-89	-0.27198632085382
2216	90-91	-0.30163938148588443
2216	92-93	-0.32065455730781167
2216	94-95	-0.3151054408544667
2216	96-97	-0.31534121484599353
2216	98-99	-0.29931436002328127
2216	100	-0.26016046586259733
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26.0
3	2.0
4	9.0
5	16.0
6	83.0
7	171.0
8	436.0
9	761.0
10	1518.0
11	2298.0
12	3662.0
13	5852.0
14	7075.0
15	9736.0
16	12020.0
17	17446.0
18	21392.0
19	12057.0
20	7911.0
21	7374.0
22	7445.0
23	8054.0
24	8993.0
25	10496.0
26	13252.0
27	16950.0
28	21733.0
29	28110.0
30	35591.0
31	45398.0
32	58407.0
33	76236.0
34	106803.0
35	164864.0
36	311747.0
37	736867.0
38	1443035.0
39	787024.0
40	2818.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.564668204940084	20.158075747654717	13.911492104350526	23.36576394305467
2	15.298349656317162	20.31748877531423	40.616948943736475	23.767212624632137
3	18.20748842101942	25.84121478573909	30.226616744193734	25.724680049047755
4	12.288201383358627	17.067049319089048	39.25457905729268	31.39017024025964
5	12.52516187609252	38.011002992006546	35.76941442658466	13.694420705316276
6	30.151823339943203	38.57989549578155	17.80012284892312	13.468158315352133
7	26.813037620037644	32.93333930949762	22.634096237445362	17.619526833019375
8	25.102068347804195	36.28878935730918	20.264121439894645	18.345020854991983
9	25.09023774688459	16.857375156405833	21.45225743980958	36.6001296569
10-11	23.824076012327513	26.98912378294841	28.702273198473183	20.484527006250893
12-13	24.83363026192733	24.22810876726015	29.202299141264977	21.735961829547545
14-15	21.819821917739617	26.02550041816279	26.754280534647418	25.40039712945018
16-17	20.96461198076555	29.72322686813225	27.20766974120032	22.104491409901875
18-19	20.87116775641407	28.14091075547821	30.04561602288626	20.942305465221462
20-21	21.842326402695466	27.412694295069095	29.83833408285316	20.906645219382284
22-23	21.54737392714408	27.183215008377022	29.884694827780912	21.38471623669799
24-25	21.3139923130537	27.32133621507224	29.895871036383102	21.468800435490962
26-27	21.111886830061792	27.34415144081726	29.918781558099962	21.62518017102099
28-29	21.21842713212916	27.414948533960676	29.945924004125345	21.420700329784818
30-31	21.409754182676025	27.707430866812377	29.59979164097135	21.28302330954025
32-33	21.158192454733044	27.481310135608343	29.955335659984527	21.405161749674086
34-35	21.26978915073848	27.30168020955314	29.934857144431447	21.493673495276926
36-37	21.564447289959766	27.59621336054608	29.43150812061478	21.407831228879378
38-39	21.44086189042154	27.773215947046914	29.467352961952997	21.318569200578548
40-41	21.359405965274487	27.5158560368432	29.597881160236128	21.526856837646182
42-43	21.332176887589064	27.505322022448713	29.443525423932666	21.71897566602956
44-45	21.454826883910386	27.650333698887668	29.34507284975717	21.549766567444774
46-47	21.567265893173047	27.8118150845043	28.983073069100772	21.637845953221877
48-49	21.570713714889056	27.839946836618363	28.790986791376344	21.798352657116244
50-51	21.66155801458833	27.844232382984085	28.653603307473592	21.840606294953997
52-53	21.678816527821855	27.813004938654107	28.60574139219812	21.902437141325922
54-55	21.714109876556364	28.143030921387012	28.438312964120062	21.70454623793656
56-57	21.580930620298236	27.925489118801643	28.552988350578463	21.94059191032166
58-59	21.437057719975083	27.558863006301802	28.908007007934867	22.096072265788248
60-61	21.690224363914606	27.908972727006155	28.527182141513602	21.87362076756564
62-63	21.461455919646088	27.918144353383173	28.60412598994724	22.016273737023496
64-65	21.520617136927754	27.659822999808696	28.76161713993251	22.057942723331035
66-67	21.409186270310755	27.73184741389898	28.630445231217983	22.228521084572282
68-69	21.74376168256476	27.822063192581247	28.510290672853134	21.923884452000856
70-71	21.68104881039796	27.667613791083006	28.525434328220868	22.12590307029817
72-73	21.537581491501044	27.81921281388438	28.578164233982395	22.06504146063218
74-75	21.619498666388896	27.947190402407013	28.372601327902068	22.06070960330203
76-77	21.70394591134385	27.83290187923851	28.284942742450514	22.178209466967125
78-79	21.73323686391941	27.885503208642987	28.378145746521398	22.00311418091621
80-81	21.669559795751137	27.814453292501923	28.366804113772904	22.14918279797404
82-83	21.555787255960254	27.832543707368394	28.491512678961644	22.120156357709703
84-85	21.596787714952768	27.753245888241086	28.351618111470454	22.29834828533569
86-87	21.66357007642151	27.870151040153846	28.280490980659252	22.185787902765398
88-89	21.456490722531296	27.755308797407086	28.334314890573896	22.453885589487722
90-91	21.42900694012249	27.77477510299759	28.372797744626077	22.42342021225384
92-93	21.536037103049505	27.864859423772938	28.25198803896401	22.347115434213553
94-95	21.349112787557793	27.636046586165175	28.37847853783223	22.636362088444802
96-97	21.430793702264154	27.610166870844584	28.395837650073897	22.563201776817365
98-99	21.365103971500876	27.72697720252805	28.256465581189616	22.651453244781454
100	21.35780941229967	27.58867286915187	28.250796010083963	22.8027217084645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	57.0
1	54.5
2	181.0
3	327.0
4	222.5
5	152.5
6	460.0
7	1098.5
8	1947.0
9	2308.0
10	1903.0
11	1534.5
12	1579.5
13	1826.5
14	2081.0
15	2372.0
16	2645.5
17	2991.0
18	3426.5
19	3932.5
20	4749.0
21	5791.5
22	7448.0
23	10141.0
24	13924.0
25	18747.0
26	25119.0
27	33290.0
28	43300.0
29	54796.0
30	66071.5
31	77368.0
32	88397.0
33	100127.5
34	110759.5
35	119132.0
36	127347.0
37	134710.0
38	140551.0
39	143110.0
40	145047.5
41	147688.5
42	148397.0
43	147410.0
44	144407.5
45	141194.0
46	139092.5
47	135508.0
48	131599.5
49	128046.0
50	123285.0
51	117499.0
52	109466.5
53	102839.5
54	98293.5
55	92557.0
56	85045.5
57	77117.5
58	69720.0
59	63244.0
60	56311.0
61	47990.5
62	40902.5
63	33844.5
64	26973.0
65	21307.0
66	16464.0
67	12525.5
68	9682.0
69	7389.0
70	5298.0
71	3701.5
72	2664.0
73	1862.5
74	1290.0
75	847.0
76	488.5
77	305.5
78	184.5
79	102.5
80	57.5
81	29.5
82	14.0
83	10.0
84	5.0
85	1.5
86	2.5
87	1.5
88	0.5
89	1.5
90	1.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.2551529070518634E-4
2	3.755945661982919E-4
3	2.253567397189751E-4
4	0.012219343220317765
5	6.760702191569254E-4
6	0.003630747473250155
7	6.259909436638198E-4
8	0.1415741118190095
9	0.0012770215250741924
10-11	0.0
12-13	7.511891323965837E-5
14-15	2.0031710197242235E-4
16-17	0.0
18-19	1.3771800760604036E-4
20-21	0.0
22-23	1.2519818873276396E-4
24-25	3.630747473250155E-4
26-27	0.00600951305917267
28-29	0.00767464896931843
30-31	0.01403471695694284
32-33	0.016563720369344673
34-35	0.02457640444824157
36-37	0.03558132523785152
38-39	0.04021365822096379
40-41	0.051318737561559954
42-43	0.06770718046667876
44-45	0.10749516484595113
46-47	0.11470658051695834
48-49	0.11157662579863925
50-51	0.11319168243329189
52-53	0.10822131434060117
54-55	0.11519485345301614
56-57	0.0987062519969111
58-59	0.09063096882364784
60-61	0.09207074799407462
62-63	0.09063096882364784
64-65	0.0
66-67	3.7559456619829185E-5
68-69	7.511891323965837E-5
70-71	1.2519818873276397E-5
72-73	0.0
74-75	0.0
76-77	6.259909436638198E-5
78-79	3.7559456619829185E-5
80-81	2.629161963388043E-4
82-83	1.2519818873276396E-4
84-85	0.0
86-87	7.011098569034783E-4
88-89	3.5055492845173915E-4
90-91	1.6275764535259317E-4
92-93	8.513476833827949E-4
94-95	9.014269588759004E-4
96-97	2.629161963388043E-4
98-99	3.7559456619829185E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3993668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.92606169423043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2423475812254	43.15883092201556
2	15.595816716952799	18.38000116070142
3	5.208947416355772	9.208282704545473
4	2.1760371872586575	5.129012061813733
5	1.1454753730220357	3.374917624995904
6	0.6380542420835568	2.255881417996866
7	0.431575736128497	1.7801740916988509
8	0.28875122384417484	1.361197794442111
9	0.2110087798044545	1.1190524739103413
>10	0.9967328307496011	10.196399755029056
>50	0.05060334004280829	2.027815522554627
>100	0.013970301404734549	1.3058145768397125
>500	4.6699892723330494E-4	0.1778689746907038
>1k	1.2736332015551895E-4	0.15637957633505298
>5k	8.490888010364928E-5	0.3683713424305324
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8307	0.20800427076061406	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6418	0.16070439505737583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.5039637746552794E-5	0.0
7	0.0	0.0	0.0	5.007927549310559E-5	0.0
8	0.0	0.0	0.0	5.007927549310559E-5	0.0
9	0.0	0.0	0.0	5.007927549310559E-5	0.0
10-11	0.0	0.0	0.0	1.2519818873276399E-4	0.0
12-13	0.0	0.0	0.0	1.7527746422586955E-4	0.0
14-15	0.0	0.0	0.0	2.3787655859225154E-4	0.0
16-17	0.0	0.0	0.0	2.879558340853571E-4	0.0
18-19	0.0	0.0	0.0	3.255152907051863E-4	0.0
20-21	0.0	0.0	0.0	3.380351095784627E-4	0.0
22-23	0.0	0.0	0.0	4.2567384169139746E-4	0.0
24-25	0.0	0.0	0.0	5.884314870439906E-4	0.0
26-27	0.0	0.0	0.0	7.386693135233073E-4	0.0
28-29	0.0	0.0	0.0	0.0015274179025397204	0.0
30-31	0.0	0.0	0.0	0.0045697338887458845	0.0
32-33	0.0	0.0	0.0	0.011580832457780667	0.0
34-35	0.0	0.0	0.0	0.022009841579219903	0.0
36-37	0.0	0.0	0.0	0.03943742945082065	0.0
38-39	0.0	0.0	0.0	0.07375425298247125	0.0
40-41	0.0	0.0	0.0	0.11505713544541007	0.0
42-43	0.0	0.0	0.0	0.15677317193116702	0.0
44-45	0.0	0.0	0.0	0.20620141684286225	0.0
46-47	0.0	0.0	0.0	0.2659209528683907	0.0
48-49	0.0	0.0	0.0	0.33779723301987047	0.0
50-51	0.0	0.0	0.0	0.41440600470544875	0.0
52-53	0.0	0.0	0.0	0.49432000857357195	0.0
54-55	0.0	0.0	0.0	0.5824970928980576	0.0
56-57	0.0	0.0	0.0	0.6846462950851198	0.0
58-59	0.0	0.0	0.0	0.8002042232854609	0.0
60-61	0.0	0.0	0.0	0.9288203225706293	0.0
62-63	0.0	0.0	0.0	1.0588010821129847	0.0
64-65	0.0	0.0	0.0	1.199761722807204	0.0
66-67	0.0	0.0	0.0	1.356735211840343	0.0
68-69	0.0	0.0	0.0	1.5361442162943941	0.0
70-71	0.0	0.0	0.0	1.7339323148544146	0.0
72-73	0.0	0.0	0.0	1.940922480286293	0.0
74-75	0.0	0.0	0.0	2.151681111199028	0.0
76-77	0.0	0.0	0.0	2.3742334114903896	0.0
78-79	0.0	0.0	0.0	2.6273465896514185	0.0
80-81	0.0	0.0	0.0	2.908616840458446	0.0
82-83	0.0	0.0	0.0	3.193680596384076	0.0
84-85	0.0	0.0	0.0	3.482813293443521	0.0
86-87	0.0	0.0	0.0	3.7872452091661097	0.0
88	0.0	0.0	0.0	4.03581369307614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5975	0.0	50.16556	1
GTATCAA	11175	0.0	42.797974	1
ATCAACG	13375	0.0	35.14019	3
TCAACGC	13425	0.0	35.009315	4
CAACGCA	13705	0.0	34.394455	5
TATCAAC	14280	0.0	33.209267	2
AACGCAG	14270	0.0	33.06521	6
ACGCAGA	16470	0.0	28.476038	7
CGCAGAG	16825	0.0	27.707102	8
GTACATG	12210	0.0	25.279741	1
GCAGAGT	18490	0.0	24.897303	9
TACATGG	12300	0.0	23.943974	2
TACACCG	610	0.0	23.136154	5
ACATGGG	12565	0.0	22.278547	3
GAGTACT	11495	0.0	20.984041	12-13
ACACCGT	740	0.0	20.9786	6
AGAGTAC	16720	0.0	20.930408	10-11
GTGGTAT	3085	0.0	20.102112	1
CAGAGTA	18265	0.0	20.00864	10-11
AGTACTT	12240	0.0	19.092789	12-13
>>END_MODULE