FastQCFastQC Report
Wed 25 May 2016
SRR1032980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866807
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77350.034.0890351
GGTATCA48150.033.091431
TCAACGC77600.032.5255554
CAACGCA79950.031.5688725
ATCAACG82500.030.6507053
AACGCAG84750.029.8915086
GTACATG162100.028.6184641
TATCAAC92500.028.1501162
TACATGG161750.026.9369012
ACATGGG166250.025.2182753
ACGCAGA100900.024.4083777
CGCAGAG103000.023.6369428
CATGGGG115450.020.1115324
GCAGAGT122500.019.2988159
GAGTACA115400.018.7354531
AGTACAT121000.016.8972512
TATACTG27500.016.4074755
AGAGTAC99700.016.1694610-11
CATGGGA58600.015.72065454
CAGAGTA115400.015.45619210-11
Produced by FastQC (version 0.11.5)