FastQCFastQC Report
Wed 25 May 2016
SRR1032980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4866807
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102840.21130897526858988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82680.16988551220543574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75150.048.8838121
GTATCAA136650.041.2189451
TCAACGC159350.034.89694
ATCAACG161550.034.4507943
CAACGCA162850.034.1151925
AACGCAG169200.032.9182746
TATCAAC170550.032.687642
ACGCAGA194800.028.398487
GTACATG159950.027.7839641
CGCAGAG200950.027.365218
TACATGG163600.026.3695662
ACATGGG164400.025.2399333
GCAGAGT220950.024.5332169
CATGGGG113800.021.2905254
GAGTACT137150.021.25123812-13
CAGAGTA215350.020.43774210-11
AGAGTAC196400.020.37681810-11
GAGTACA113450.020.1656281
GTACTTT147450.019.5915414-15
GTGGTAT39300.019.4841921
Produced by FastQC (version 0.11.5)