##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032980_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4866807
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83790419468041	34.0	31.0	34.0	31.0	34.0
2	32.895986013006066	34.0	31.0	34.0	31.0	34.0
3	32.97998174162238	34.0	31.0	34.0	31.0	34.0
4	36.3871145907368	37.0	37.0	37.0	35.0	37.0
5	36.36136177169138	37.0	37.0	37.0	35.0	37.0
6	36.41791219581956	37.0	37.0	37.0	35.0	37.0
7	36.39000026095138	37.0	37.0	37.0	35.0	37.0
8	36.32460769453155	37.0	37.0	37.0	35.0	37.0
9	38.167824407255104	39.0	39.0	39.0	37.0	39.0
10-11	38.18482271846818	39.0	39.0	39.0	37.0	39.0
12-13	38.17615214657167	39.0	39.0	39.0	37.0	39.0
14-15	39.73974425121029	41.0	40.0	41.0	37.0	41.0
16-17	39.74694198886456	41.0	40.0	41.0	37.0	41.0
18-19	39.77090801011833	41.0	40.0	41.0	37.0	41.0
20-21	39.77664483099494	41.0	40.0	41.0	37.0	41.0
22-23	39.74057313141861	41.0	40.0	41.0	37.0	41.0
24-25	39.720446485755446	41.0	40.0	41.0	37.0	41.0
26-27	39.646397936059515	41.0	40.0	41.0	37.0	41.0
28-29	39.58840272482554	41.0	40.0	41.0	37.0	41.0
30-31	39.480157935171874	41.0	40.0	41.0	36.5	41.0
32-33	39.363898342383415	41.0	39.0	41.0	35.5	41.0
34-35	39.27515545613376	41.0	39.0	41.0	35.0	41.0
36-37	39.189232591306784	41.0	39.0	41.0	35.0	41.0
38-39	39.09612842670769	41.0	39.0	41.0	35.0	41.0
40-41	38.997706607227286	41.0	39.0	41.0	35.0	41.0
42-43	38.86076363825399	41.0	39.0	41.0	35.0	41.0
44-45	38.688589870114015	41.0	39.0	41.0	35.0	41.0
46-47	38.46660151101122	41.0	38.5	41.0	35.0	41.0
48-49	38.262072032032506	41.0	38.0	41.0	34.5	41.0
50-51	38.10117814410968	40.5	38.0	41.0	34.0	41.0
52-53	37.95695678912273	40.0	38.0	41.0	34.0	41.0
54-55	37.80125531996646	40.0	37.0	41.0	34.0	41.0
56-57	37.581788531988224	40.0	37.0	41.0	33.0	41.0
58-59	37.39974093897703	40.0	36.5	41.0	33.0	41.0
60-61	37.19848413960118	39.5	36.0	41.0	33.0	41.0
62-63	36.950204518075196	39.0	35.0	41.0	33.0	41.0
64-65	36.6541754172705	39.0	35.0	41.0	32.0	41.0
66-67	36.36802178512524	38.5	35.0	41.0	32.0	41.0
68-69	36.06488658785935	37.0	35.0	40.0	32.0	41.0
70-71	35.7343737896325	37.0	35.0	40.0	31.5	41.0
72-73	35.38778515359249	36.5	35.0	39.0	31.0	41.0
74-75	35.04009456302664	36.0	35.0	39.0	31.0	41.0
76-77	34.69512824321984	35.5	35.0	38.5	31.0	40.0
78-79	34.359678943504434	35.0	35.0	37.0	31.0	39.0
80-81	34.04138863530031	35.0	34.5	37.0	30.5	39.0
82-83	33.74616057304101	35.0	34.0	36.0	30.0	38.5
84-85	33.480784115745706	35.0	34.0	36.0	30.0	37.0
86-87	33.34717557117017	35.0	34.0	36.0	30.0	37.0
88-89	33.169670484159326	35.0	34.0	35.0	30.0	37.0
90-91	33.0088228277801	35.0	34.0	35.0	30.0	36.0
92-93	32.85905718061144	35.0	34.0	35.0	30.0	36.0
94-95	32.765130300009844	35.0	34.0	35.0	30.0	36.0
96-97	32.7123357264835	35.0	34.0	35.0	30.0	36.0
98-99	32.604573902355284	35.0	34.0	35.0	30.0	35.5
100	32.523149572193844	35.0	34.0	35.0	30.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15951001522086727
1101	2	0.20593948024120579
1101	3	0.1667468355325994
1101	4	0.10058670628090738
1101	5	0.10057169540440469
1101	6	0.11172042735080367
1101	7	0.08303534252116407
1101	8	0.03645418219254282
1101	9	0.1673238791070375
1101	10-11	0.15636499440851281
1101	12-13	0.18820851086991297
1101	14-15	0.27574593393357105
1101	16-17	0.2526208893839694
1101	18-19	0.2535040372984554
1101	20-21	0.2740967089612951
1101	22-23	0.275350266295618
1101	24-25	0.25470604180925704
1101	26-27	0.2645083470058296
1101	28-29	0.2620117761141998
1101	30-31	0.2488625928982131
1101	32-33	0.22200935583862247
1101	34-35	0.2550271583262358
1101	36-37	0.275496314769363
1101	38-39	0.29137385081988043
1101	40-41	0.35018430107854925
1101	42-43	0.3172092526518284
1101	44-45	0.27920956754767445
1101	46-47	0.2743221249249217
1101	48-49	0.26532731533797005
1101	50-51	0.27510077712081227
1101	52-53	0.31045798232560173
1101	54-55	0.24054513079173745
1101	56-57	0.2895161050038553
1101	58-59	0.2789019685712546
1101	60-61	0.2735715853084102
1101	62-63	0.26132100296999283
1101	64-65	0.31787096975784124
1101	66-67	0.36808887525486256
1101	68-69	0.3309042905757025
1101	70-71	0.4144238305102803
1101	72-73	0.40328987594506316
1101	74-75	0.3681564172703986
1101	76-77	0.36103683623374394
1101	78-79	0.36669436191047566
1101	80-81	0.3391905589188582
1101	82-83	0.34198367345902625
1101	84-85	0.3269600717868144
1101	86-87	0.3637002837629595
1101	88-89	0.39486030664797056
1101	90-91	0.3519682604189285
1101	92-93	0.42171720198449947
1101	94-95	0.3859074967404936
1101	96-97	0.3466343658056985
1101	98-99	0.3695547657717171
1101	100	0.3710618103766379
1102	1	0.19056670935774633
1102	2	0.19353646789073764
1102	3	0.21316824634936182
1102	4	0.11101198673677715
1102	5	0.13255619494693605
1102	6	0.15834428585917237
1102	7	0.1535063254179434
1102	8	0.05400824951701111
1102	9	0.12808776439169378
1102	10-11	0.1869833826771341
1102	12-13	0.20868605837376464
1102	14-15	0.266785273781629
1102	16-17	0.2563973008058653
1102	18-19	0.24922249903820415
1102	20-21	0.2894927714974216
1102	22-23	0.29100018692147245
1102	24-25	0.2545914948338748
1102	26-27	0.30851682270633773
1102	28-29	0.28395939636713763
1102	30-31	0.28655527780425416
1102	32-33	0.31933332313353446
1102	34-35	0.3110640816460446
1102	36-37	0.33331278843126455
1102	38-39	0.30560052356560163
1102	40-41	0.2716860755756798
1102	42-43	0.2718613461569319
1102	44-45	0.28582033790631556
1102	46-47	0.26376828995675083
1102	48-49	0.23268512315388534
1102	50-51	0.24250912841888805
1102	52-53	0.25511926856197675
1102	54-55	0.25838166443281096
1102	56-57	0.24446851645038947
1102	58-59	0.228759127055703
1102	60-61	0.2832083714729734
1102	62-63	0.2868971456251259
1102	64-65	0.26477755693895944
1102	66-67	0.3003965633508159
1102	68-69	0.23949238218453672
1102	70-71	0.24797238333665916
1102	72-73	0.2244403213402535
1102	74-75	0.23951107270609384
1102	76-77	0.29630143780772755
1102	78-79	0.24358577007744486
1102	80-81	0.2627066582897868
1102	82-83	0.2704508097584082
1102	84-85	0.3033129654439435
1102	86-87	0.2724553365427411
1102	88-89	0.2305556856094455
1102	90-91	0.24155382781943047
1102	92-93	0.27223825840373195
1102	94-95	0.2738811593771757
1102	96-97	0.25403021982572227
1102	98-99	0.3166492341748537
1102	100	0.2981675692195509
1103	1	0.15530440916010946
1103	2	0.17198348693273857
1103	3	0.16947845948839557
1103	4	0.11343270283594364
1103	5	0.11765721130206686
1103	6	0.1271890136304279
1103	7	0.1499819115956882
1103	8	0.07919131397055423
1103	9	0.10972886945431526
1103	10-11	0.13433566940738473
1103	12-13	0.16019088808323545
1103	14-15	0.2016936906462874
1103	16-17	0.24081922440118575
1103	18-19	0.22540196797104528
1103	20-21	0.21237807091959837
1103	22-23	0.21544235286358315
1103	24-25	0.167647258151419
1103	26-27	0.1939668241648036
1103	28-29	0.22258402851834092
1103	30-31	0.2551105146997017
1103	32-33	0.2869357167717723
1103	34-35	0.24812689401137078
1103	36-37	0.26060225674219595
1103	38-39	0.2775517195866968
1103	40-41	0.25542709021368637
1103	42-43	0.24698197194153693
1103	44-45	0.24533003062220615
1103	46-47	0.298421338444264
1103	48-49	0.2936699535017766
1103	50-51	0.2587085645170717
1103	52-53	0.2811653570504191
1103	54-55	0.27406640116397085
1103	56-57	0.34109462687629133
1103	58-59	0.3144200826067376
1103	60-61	0.28620658334397575
1103	62-63	0.3042459595655913
1103	64-65	0.32754005939495556
1103	66-67	0.3641607812879144
1103	68-69	0.3566687674341509
1103	70-71	0.3499471403927288
1103	72-73	0.3494462790184443
1103	74-75	0.3236319478050902
1103	76-77	0.3009191474946036
1103	78-79	0.3046157852179192
1103	80-81	0.31561948135967555
1103	82-83	0.24584573640328955
1103	84-85	0.23357769549370033
1103	86-87	0.297451607834212
1103	88-89	0.33039932751474055
1103	90-91	0.3490701336957045
1103	92-93	0.3367265337480774
1103	94-95	0.35007202082167055
1103	96-97	0.2833433724529044
1103	98-99	0.3071188202964663
1103	100	0.31691017715809267
1104	1	0.09023754365667713
1104	2	0.11721249547152723
1104	3	0.14361563978192748
1104	4	0.08028811897175814
1104	5	0.0788902395816038
1104	6	0.09057433607453902
1104	7	0.11110458041009252
1104	8	0.07701511385880622
1104	9	0.1574797340685521
1104	10-11	0.15217279464767586
1104	12-13	0.15970075292293018
1104	14-15	0.22738872601981797
1104	16-17	0.18615925645918452
1104	18-19	0.15464668866199105
1104	20-21	0.18484464923544408
1104	22-23	0.20714142378867706
1104	24-25	0.16536009776369553
1104	26-27	0.20718241754614297
1104	28-29	0.25232612572131785
1104	30-31	0.25264703214076434
1104	32-33	0.27257399765590407
1104	34-35	0.23974605709452135
1104	36-37	0.2337371264437209
1104	38-39	0.20993030124938628
1104	40-41	0.230853136249074
1104	42-43	0.2561910424144358
1104	44-45	0.21543583341142636
1104	46-47	0.2615080895216977
1104	48-49	0.24865445416313747
1104	50-51	0.21393579275847685
1104	52-53	0.21868403357165533
1104	54-55	0.16932568740701726
1104	56-57	0.13881164054284767
1104	58-59	0.146906557247938
1104	60-61	0.16352164190880814
1104	62-63	0.1397835730121244
1104	64-65	0.19784194786991094
1104	66-67	0.1792394574676237
1104	68-69	0.1856556137875316
1104	70-71	0.1900148992070143
1104	72-73	0.16036898849826287
1104	74-75	0.1803413763928745
1104	76-77	0.1941367203895581
1104	78-79	0.24131843412968124
1104	80-81	0.24795964275011784
1104	82-83	0.1716154304293056
1104	84-85	0.2276388068103543
1104	86-87	0.1940340376587173
1104	88-89	0.23127388674883775
1104	90-91	0.26694833542458696
1104	92-93	0.27731510069722276
1104	94-95	0.2602092491800718
1104	96-97	0.2557975234708181
1104	98-99	0.28106731436409405
1104	100	0.2577740103375845
1105	1	0.03977931701591331
1105	2	0.07766466182996368
1105	3	0.09034643187109026
1105	4	0.03335239132963608
1105	5	0.021064651394951284
1105	6	0.038946782129926305
1105	7	0.04119935212528958
1105	8	-0.027169107655581115
1105	9	0.026628626126068866
1105	10-11	0.03365927579077521
1105	12-13	0.05349247049965555
1105	14-15	0.08059092875117813
1105	16-17	0.10391710077922056
1105	18-19	0.09997904814741077
1105	20-21	0.07769020964553874
1105	22-23	0.09241624307500018
1105	24-25	0.08758317951510719
1105	26-27	0.08329086933008512
1105	28-29	0.07544628726040514
1105	30-31	0.11345359187505011
1105	32-33	0.14849376741008768
1105	34-35	0.13215220492850932
1105	36-37	0.1186364664857038
1105	38-39	0.1666847710284145
1105	40-41	0.16671085066925428
1105	42-43	0.14417835135233048
1105	44-45	0.1024427777923691
1105	46-47	0.1418396100638617
1105	48-49	0.150674653206039
1105	50-51	0.1498643180806809
1105	52-53	0.11358126542781832
1105	54-55	0.14970267610758725
1105	56-57	0.13171485023070062
1105	58-59	0.13913895319451086
1105	60-61	0.21865024004102196
1105	62-63	0.24463765583459463
1105	64-65	0.19147546328441223
1105	66-67	0.18880264874363917
1105	68-69	0.13582689536400494
1105	70-71	0.12938536773036446
1105	72-73	0.16605338052826824
1105	74-75	0.17546625181461195
1105	76-77	0.16180293662551293
1105	78-79	0.1953969776664337
1105	80-81	0.2232031568604924
1105	82-83	0.17489333455653622
1105	84-85	0.21744729033939336
1105	86-87	0.2084308474766985
1105	88-89	0.2046647077470567
1105	90-91	0.19269482850539532
1105	92-93	0.2327369660450671
1105	94-95	0.24630882749477934
1105	96-97	0.22965304164019784
1105	98-99	0.14277865556167768
1105	100	0.1352685231192794
1106	1	0.06651646254061205
1106	2	0.09643020015175097
1106	3	0.0718792354820934
1106	4	0.021362130601254137
1106	5	0.02733687125279971
1106	6	0.04625412078755886
1106	7	0.019050810154162434
1106	8	0.043542738657855296
1106	9	0.05417513377140892
1106	10-11	0.0681438681762998
1106	12-13	0.03146249982917482
1106	14-15	0.06623841613973269
1106	16-17	0.07479727795033142
1106	18-19	0.10666862359715168
1106	20-21	0.06818425326962085
1106	22-23	0.08780015559788268
1106	24-25	0.09550289614720953
1106	26-27	0.07152767312325636
1106	28-29	0.10471303079743421
1106	30-31	0.13902644992922575
1106	32-33	0.11501077074616717
1106	34-35	0.09763343554359238
1106	36-37	0.08457392253835394
1106	38-39	0.11115545566391916
1106	40-41	0.09404108733949812
1106	42-43	0.1308962692856781
1106	44-45	0.13293360412413335
1106	46-47	0.11244700592805401
1106	48-49	0.05034658093805433
1106	50-51	0.06961581956070262
1106	52-53	0.08138337779333682
1106	54-55	0.0656103325863242
1106	56-57	0.08061310070683447
1106	58-59	0.028500255276256325
1106	60-61	0.03622068735698036
1106	62-63	0.023121405661882477
1106	64-65	0.058126658944722465
1106	66-67	-0.005909775174551157
1106	68-69	0.06561110598264008
1106	70-71	0.09377874272385611
1106	72-73	0.11036912430439827
1106	74-75	0.11421016380820248
1106	76-77	0.10793333097537783
1106	78-79	0.00852241483198668
1106	80-81	0.09140495355899958
1106	82-83	0.12906763286830625
1106	84-85	0.15150210647675522
1106	86-87	0.12700809880431052
1106	88-89	0.08245114292869715
1106	90-91	0.1378545922259633
1106	92-93	0.0961219599228258
1106	94-95	0.11470858922128713
1106	96-97	0.0988613961682745
1106	98-99	0.1263157468955498
1106	100	0.1603788665525414
1107	1	0.02130879444843714
1107	2	0.055223238320124324
1107	3	0.023686258667510174
1107	4	0.015318123183888588
1107	5	0.020046510810168172
1107	6	0.032124895309479484
1107	7	-0.005659502955538187
1107	8	-0.024523103118099243
1107	9	0.004666428484853213
1107	10-11	0.03163009056408583
1107	12-13	0.037356249279028475
1107	14-15	0.053594333974480435
1107	16-17	0.05054402878365494
1107	18-19	0.0690806365680956
1107	20-21	0.055745096923892845
1107	22-23	0.03310297165271692
1107	24-25	0.047434221633011475
1107	26-27	0.06390381103321374
1107	28-29	0.05865142495675002
1107	30-31	0.09420178055317052
1107	32-33	0.09841925341338253
1107	34-35	0.05966516006586886
1107	36-37	0.142766620741142
1107	38-39	0.1530784183558751
1107	40-41	0.13028061918584655
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2216	24-25	-0.32187888019099375
2216	26-27	-0.3378835522545103
2216	28-29	-0.34644415625062663
2216	30-31	-0.3768976773859123
2216	32-33	-0.4768085138903473
2216	34-35	-0.4674214086973265
2216	36-37	-0.44016964637685163
2216	38-39	-0.41483642254848974
2216	40-41	-0.3837721179599498
2216	42-43	-0.3812421773395229
2216	44-45	-0.388934223761332
2216	46-47	-0.3303966560748748
2216	48-49	-0.39461476048555255
2216	50-51	-0.31797488233214466
2216	52-53	-0.36123457464130837
2216	54-55	-0.3396562255345046
2216	56-57	-0.37436598410489097
2216	58-59	-0.38243648547666
2216	60-61	-0.4377197879924566
2216	62-63	-0.3465285811753631
2216	64-65	-0.41444943379151766
2216	66-67	-0.4416362209505067
2216	68-69	-0.453982514167933
2216	70-71	-0.4344495226107128
2216	72-73	-0.4055549342876361
2216	74-75	-0.44271602052675973
2216	76-77	-0.445903131503961
2216	78-79	-0.4328720602522722
2216	80-81	-0.45478546843840206
2216	82-83	-0.44913520033770027
2216	84-85	-0.4418807661323356
2216	86-87	-0.39798488914870944
2216	88-89	-0.3811678100226459
2216	90-91	-0.40410839699431733
2216	92-93	-0.42056602464947446
2216	94-95	-0.3935175887636646
2216	96-97	-0.36002416163560014
2216	98-99	-0.33655151278733086
2216	100	-0.3616330507660521
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	27.0
3	11.0
4	17.0
5	37.0
6	90.0
7	259.0
8	550.0
9	1037.0
10	1762.0
11	2841.0
12	4576.0
13	6907.0
14	8260.0
15	11074.0
16	14395.0
17	21228.0
18	25762.0
19	14394.0
20	9853.0
21	9388.0
22	9627.0
23	10209.0
24	11397.0
25	13468.0
26	16948.0
27	21425.0
28	27758.0
29	35612.0
30	45927.0
31	58011.0
32	74493.0
33	97503.0
34	137360.0
35	214486.0
36	418938.0
37	982675.0
38	1730666.0
39	825256.0
40	2580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19327244170077	19.562368445060685	13.434445880938311	23.809913232300236
2	15.69873392700432	20.082021360731044	39.936808333886404	24.282436378378232
3	18.329611857655046	25.55752787716439	29.933013437790812	26.179846827389756
4	12.465394055668717	16.675708599864862	38.369340777310335	32.48955656715608
5	12.770219512515862	38.07923261028227	35.40516694392026	13.74538093328161
6	30.970448580908865	37.918985516895034	17.493788317022272	13.616777585173825
7	27.69476580761606	32.59501993931085	22.18067514210663	17.529539110966457
8	25.73223436108555	35.869278049471035	19.887281134209154	18.511206455234262
9	25.804321949461436	16.222875317538087	20.6566450347964	37.31615769820407
10-11	24.383358534661433	26.46604847901304	28.43001581940685	20.72057716691868
12-13	25.206123654775585	23.888512358070226	28.728048073482377	22.17731591367181
14-15	22.266049793092975	25.544179496196627	26.224020529302678	25.96575018140772
16-17	21.59575056089136	29.117427504316485	26.748913610093844	22.537908324698307
18-19	21.504910051311924	27.70227474815343	29.36508107736034	21.427734123174304
20-21	22.436969454510937	26.914730746462723	29.193349972579558	21.454949826446786
22-23	22.206244349469877	26.63792430344374	29.217545409714802	21.938285937371578
24-25	21.930988824978428	26.829226195533547	29.21958953861081	22.02019544087722
26-27	21.699942032416228	26.925932387317157	29.25145787137525	22.122667708891356
28-29	21.84177250370371	26.964486819786558	29.32872719479032	21.86501348171941
30-31	21.972519682595983	27.14592237193597	29.003436221319696	21.878121724148347
32-33	21.816799488939857	26.930123910711618	29.229288300251326	22.0237883000972
34-35	22.008587762725853	26.78284864458698	29.230393905861813	21.978169686825353
36-37	22.19209993209638	27.010071051628437	28.776664915718627	22.021164100556557
38-39	21.978817907907388	27.270928608980267	28.847585859213964	21.902667623898385
40-41	21.962647948153492	26.984651548331446	29.010234768114167	22.042465735400903
42-43	22.00994775270849	27.043504454693313	28.768580070362155	22.177967722236044
44-45	22.002016868259215	27.144598963590948	28.74250166743889	22.110882500710943
46-47	22.20861662097962	27.25642268092241	28.359299231879525	22.175661466218447
48-49	22.16733553389778	27.368588648144225	28.131733948857807	22.332341869100187
50-51	22.17855673496256	27.38711634987246	28.008002139389447	22.426324775775527
52-53	22.289587541380236	27.34988875277321	27.997830697076264	22.36269300877029
54-55	22.183030358385956	27.652635355415452	27.86909746050711	22.295236825691486
56-57	22.134942110195073	27.397101930976497	28.000845743188602	22.46711021563983
58-59	22.098468517049177	27.03879978923754	28.258550502165004	22.604181191548278
60-61	22.20966746612401	27.350788671885162	28.032951367333254	22.406592494657573
62-63	22.025532688537723	27.325928631541018	28.090101507182997	22.55843717273826
64-65	22.062072730642495	27.145097391369745	28.15795859585145	22.634871282136316
66-67	21.9345215254647	27.191953736388275	28.09580123964272	22.777723498504308
68-69	22.211821827575704	27.30932681159755	27.965599590285407	22.513251770541338
70-71	22.22647438315043	27.154685521193418	27.9792611438322	22.639578951823953
72-73	22.092369802213238	27.289555554596678	27.946516062790245	22.67155858039984
74-75	22.133556970720228	27.396977114564024	27.77857227541589	22.69089363929985
76-77	22.194054064438802	27.23489863521303	27.745418527787287	22.82562877256088
78-79	22.12180042946035	27.362106416621746	27.801028826814633	22.71506432710327
80-81	22.087207503830296	27.314693480482894	27.83349492155463	22.764604094132185
82-83	22.037838004438235	27.302560203830033	27.859702473904825	22.799899317826906
84-85	22.075469604609346	27.31333911535839	27.64975064760119	22.961440632431078
86-87	22.120382002533916	27.327794551380443	27.676762882352868	22.87506056373277
88-89	21.909059938505642	27.326555009176484	27.720182181759306	23.044202870558568
90-91	21.86304911896862	27.244084198310347	27.787634821759532	23.105231860961503
92-93	21.971807920551612	27.347235541038927	27.62882962925825	23.05212690915121
94-95	21.815287785730717	27.146438144763618	27.76400576606032	23.274268303445346
96-97	21.85520804085398	27.15929256544834	27.705432105081858	23.28006728861583
98-99	21.731703009644214	27.266761999583096	27.6443197995728	23.35721519119989
100	21.76652988293968	27.084924468958803	27.652853297860386	23.495692350241136
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	72.5
2	232.0
3	426.5
4	308.0
5	238.0
6	614.0
7	1443.0
8	2485.5
9	2986.0
10	2478.5
11	1910.0
12	1974.0
13	2264.0
14	2607.5
15	2885.0
16	3221.0
17	3680.5
18	4078.5
19	4539.0
20	5277.0
21	6390.0
22	7949.0
23	10408.5
24	14066.5
25	18839.0
26	25683.5
27	34204.0
28	44596.5
29	57138.0
30	69588.0
31	81125.0
32	92136.5
33	103645.0
34	115016.5
35	124873.5
36	134442.0
37	145650.0
38	154989.0
39	159127.0
40	164364.5
41	171129.0
42	174800.0
43	177352.0
44	177845.0
45	176929.0
46	176465.5
47	173168.0
48	168005.5
49	164563.5
50	161699.0
51	155983.5
52	145799.0
53	138216.0
54	133699.0
55	125873.5
56	115007.5
57	103994.5
58	94977.0
59	88769.5
60	80877.5
61	69476.5
62	60039.0
63	51546.5
64	42255.5
65	33299.5
66	25897.5
67	19580.5
68	14516.5
69	10863.0
70	7622.0
71	5462.0
72	4188.5
73	3034.5
74	2130.0
75	1430.0
76	885.0
77	589.5
78	398.5
79	214.5
80	103.5
81	56.5
82	36.0
83	18.5
84	11.5
85	7.0
86	3.5
87	1.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.903997014880598E-4
2	5.34231170457345E-4
3	2.0547352709897887E-4
4	0.01214348545154965
5	4.725891123276514E-4
6	0.0035135973133925387
7	8.013467556860174E-4
8	0.1387151781445206
9	0.0013972199842730562
10-11	0.0
12-13	1.5410514532423412E-4
14-15	1.5410514532423412E-4
16-17	0.0
18-19	9.246308719454049E-5
20-21	0.0
22-23	1.438314689692852E-4
24-25	3.184839670034172E-4
26-27	0.006040921696709979
28-29	0.0082703094657339
30-31	0.01450643101318791
32-33	0.017331692010798868
34-35	0.026280064115959394
36-37	0.03746809766649879
38-39	0.04283095672378214
40-41	0.053484759103864195
42-43	0.06979935715552311
44-45	0.10909616921320282
46-47	0.11620555325082749
48-49	0.11256867182117555
50-51	0.11520900664439744
52-53	0.10967149508907996
54-55	0.1166884160395101
56-57	0.09885331388731873
58-59	0.09166174043885447
60-61	0.09350072850639034
62-63	0.0922781610201514
64-65	0.0
66-67	3.082102906484683E-5
68-69	9.246308719454049E-5
70-71	1.0273676354948942E-5
72-73	0.0
74-75	0.0
76-77	7.19157344846426E-5
78-79	3.082102906484683E-5
80-81	2.5684190887372357E-4
82-83	1.438314689692852E-4
84-85	0.0
86-87	6.780626394266302E-4
88-89	3.903997014880598E-4
90-91	2.2602087980887672E-4
92-93	0.0010273676354948943
94-95	0.0010787360172696391
96-97	2.157472034539278E-4
98-99	5.136838177474472E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4866807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.55306479522768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.6212528217121	32.15597816965348
2	16.48763853156676	15.680754868237731
3	6.551768907115855	9.346700740903149
4	3.0879616040502973	5.87368152970316
5	1.6780461281014476	3.989811812949453
6	1.0232303460546277	2.919464336782737
7	0.7136101558292933	2.3754044985078036
8	0.4902762524002012	1.8651310718358534
9	0.3381154801916369	1.4470584604040186
>10	1.8380716132049284	15.629597671426144
>50	0.11951321895534839	3.882866028253455
>100	0.04925983692372715	3.945699917165937
>500	9.954275035230192E-4	0.3182394037505364
>1k	1.7311759359863604E-4	0.18779870831971085
>5k	4.327939839901296E-5	0.17016106524680177
>10k+	4.327939839901296E-5	0.21165171685995518
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10284	0.21130897526858988	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8268	0.16988551220543574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.0547352709897887E-5	0.0	0.0	0.0
6	0.0	2.0547352709897887E-5	0.0	0.0	0.0
7	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
8	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
9	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
10-11	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
12-13	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
14-15	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
16-17	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
18-19	0.0	2.0547352709897887E-5	0.0	2.0547352709897887E-5	0.0
20-21	0.0	2.0547352709897887E-5	0.0	6.164205812969366E-5	0.0
22-23	0.0	2.0547352709897887E-5	0.0	1.3355779261433627E-4	0.0
24-25	0.0	2.0547352709897887E-5	0.0	1.9519985074402992E-4	0.0
26-27	0.0	2.0547352709897887E-5	0.0	3.390313197133151E-4	0.0
28-29	0.0	2.0547352709897887E-5	0.0	0.0014280410133379032	0.0
30-31	0.0	2.0547352709897887E-5	0.0	0.005979279638580285	0.0
32-33	0.0	4.1094705419795775E-5	0.0	0.014927651743740816	0.0
34-35	0.0	5.136838177474472E-5	0.0	0.028982040997310967	0.0
36-37	0.0	6.164205812969366E-5	0.0	0.05360804322012358	0.0
38-39	0.0	6.164205812969366E-5	0.0	0.09639790523848593	0.0
40-41	0.0	6.164205812969366E-5	0.0	0.14641016173437738	0.0
42-43	0.0	6.164205812969366E-5	0.0	0.19850797453032348	0.0
44-45	0.0	6.164205812969366E-5	0.0	0.2599342854565632	0.0
46-47	0.0	6.164205812969366E-5	0.0	0.33810668884136974	0.0
48-49	0.0	6.164205812969366E-5	0.0	0.425576769327405	0.0
50-51	0.0	6.164205812969366E-5	0.0	0.5209370332540411	0.0
52-53	0.0	6.164205812969366E-5	0.0	0.6194718631743564	0.0
54-55	0.0	6.164205812969366E-5	0.0	0.7267290443200234	0.0
56-57	0.0	6.164205812969366E-5	0.0	0.85416372582681	0.0
58-59	0.0	6.164205812969366E-5	0.0	1.0002964982996039	0.0
60-61	0.0	6.164205812969366E-5	0.0	1.1560865265460496	0.0
62-63	0.0	6.164205812969366E-5	0.0	1.3156367203384067	0.0
64-65	0.0	6.164205812969366E-5	0.0	1.482789434633426	0.0
66-67	0.0	6.164205812969366E-5	0.0	1.675944823782821	0.0
68-69	0.0	6.164205812969366E-5	0.0	1.8949590563176226	0.0
70-71	0.0	6.164205812969366E-5	0.0	2.1320344118844243	0.0
72-73	0.0	6.164205812969366E-5	0.0	2.374339068715895	0.0
74-75	0.0	8.218941083959155E-5	0.0	2.6218113847539053	0.0
76-77	0.0	1.0273676354948942E-4	0.0	2.8894817485057454	0.0
78-79	0.0	1.0273676354948942E-4	0.0	3.1948565044802475	0.0
80-81	0.0	1.0273676354948942E-4	0.0	3.5253298517898903	0.0
82-83	0.0	1.0273676354948942E-4	0.0	3.8744191006547	0.0
84-85	0.0	1.0273676354948942E-4	0.0	4.209782717909299	0.0
86-87	0.0	1.0273676354948942E-4	0.0	4.568015538730013	0.0
88	0.0	1.0273676354948942E-4	0.0	4.861072156754932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7515	0.0	48.883812	1
GTATCAA	13665	0.0	41.218945	1
TCAACGC	15935	0.0	34.8969	4
ATCAACG	16155	0.0	34.450794	3
CAACGCA	16285	0.0	34.115192	5
AACGCAG	16920	0.0	32.918274	6
TATCAAC	17055	0.0	32.68764	2
ACGCAGA	19480	0.0	28.39848	7
GTACATG	15995	0.0	27.783964	1
CGCAGAG	20095	0.0	27.36521	8
TACATGG	16360	0.0	26.369566	2
ACATGGG	16440	0.0	25.239933	3
GCAGAGT	22095	0.0	24.533216	9
CATGGGG	11380	0.0	21.290525	4
GAGTACT	13715	0.0	21.251238	12-13
CAGAGTA	21535	0.0	20.437742	10-11
AGAGTAC	19640	0.0	20.376818	10-11
GAGTACA	11345	0.0	20.165628	1
GTACTTT	14745	0.0	19.59154	14-15
GTGGTAT	3930	0.0	19.484192	1
>>END_MODULE