##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032979_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1478 Sequences flagged as poor quality 0 Sequence length 100 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.14005412719892 22.0 2.0 30.0 2.0 31.0 2 17.039918809201623 18.0 2.0 30.0 2.0 31.0 3 17.092016238159676 18.0 2.0 30.0 2.0 31.0 4 19.256427604871448 25.0 2.0 35.0 2.0 37.0 5 18.722598105548037 22.0 2.0 35.0 2.0 37.0 6 19.077807848443843 24.0 2.0 35.0 2.0 37.0 7 18.828146143437078 23.0 2.0 35.0 2.0 37.0 8 18.75575101488498 22.0 2.0 35.0 2.0 36.0 9 19.433694181326118 25.0 2.0 35.0 2.0 39.0 10-11 19.335588633288225 24.0 2.0 35.0 2.0 39.0 12-13 19.28450608930988 22.5 2.0 35.0 2.0 39.0 14-15 19.360284167794315 21.5 2.0 36.0 2.0 40.0 16-17 19.233761840324764 21.0 2.0 36.0 2.0 40.0 18-19 19.109945872801084 21.0 2.0 36.0 2.0 39.5 20-21 19.14445196211096 20.5 2.0 36.0 2.0 40.0 22-23 18.860960757780784 18.0 2.0 36.0 2.0 40.0 24-25 18.483085250338295 17.0 2.0 36.0 2.0 39.5 26-27 18.248308525033828 16.0 2.0 35.5 2.0 39.0 28-29 18.07510148849797 13.5 2.0 36.0 2.0 40.0 30-31 17.787550744248986 9.5 2.0 35.0 2.0 39.5 32-33 17.308186738836262 9.0 2.0 35.0 2.0 39.0 34-35 16.93809201623816 8.0 2.0 34.5 2.0 39.0 36-37 16.860960757780784 2.0 2.0 34.0 2.0 39.5 38-39 16.713125845737483 2.0 2.0 34.5 2.0 39.5 40-41 16.593031123139376 2.0 2.0 34.0 2.0 39.0 42-43 16.422192151556157 2.0 2.0 34.0 2.0 39.0 44-45 16.41238159675237 2.0 2.0 34.0 2.0 39.0 46-47 16.143775372124495 2.0 2.0 33.5 2.0 39.0 48-49 15.57916102841678 2.0 2.0 33.0 2.0 39.0 50-51 15.506427604871448 2.0 2.0 33.0 2.0 38.5 52-53 15.364343707713125 2.0 2.0 33.0 2.0 38.0 54-55 15.191813261163734 2.0 2.0 33.0 2.0 38.5 56-57 14.951962110960757 2.0 2.0 32.5 2.0 38.0 58-59 14.74424898511502 2.0 2.0 32.0 2.0 38.0 60-61 14.591001353179973 2.0 2.0 32.0 2.0 38.0 62-63 14.469553450608931 2.0 2.0 32.0 2.0 38.0 64-65 14.145805142083898 2.0 2.0 31.5 2.0 37.5 66-67 13.841677943166442 2.0 2.0 31.0 2.0 37.0 68-69 13.718200270635995 2.0 2.0 31.0 2.0 36.5 70-71 13.530784844384304 2.0 2.0 30.5 2.0 36.5 72-73 13.279431664411366 2.0 2.0 30.0 2.0 36.0 74-75 13.187077131258457 2.0 2.0 30.5 2.0 35.5 76-77 12.891745602165088 2.0 2.0 30.0 2.0 35.0 78-79 12.578822733423547 2.0 2.0 29.5 2.0 35.0 80-81 12.422868741542626 2.0 2.0 30.0 2.0 35.0 82-83 12.105886332882273 2.0 2.0 29.0 2.0 34.5 84-85 12.154939106901217 2.0 2.0 29.0 2.0 34.0 86-87 11.953315290933695 2.0 2.0 29.0 2.0 34.0 88-89 11.686738836265224 2.0 2.0 29.0 2.0 34.0 90-91 11.62280108254398 2.0 2.0 29.0 2.0 34.0 92-93 11.469553450608931 2.0 2.0 29.0 2.0 34.0 94-95 11.270974289580515 2.0 2.0 29.0 2.0 34.0 96-97 10.913734776725304 2.0 2.0 28.5 2.0 34.0 98-99 10.781123139377538 2.0 2.0 27.5 2.0 34.0 100 10.757104194857916 2.0 2.0 27.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 9.612021857923501 1102 2 11.69535519125683 1102 3 12.336065573770494 1102 4 13.106557377049178 1102 5 2.5901639344262293 1102 6 2.3975409836065573 1102 7 1.342896174863391 1102 8 -0.269125683060107 1102 9 4.685792349726775 1102 10-11 7.68510928961749 1102 12-13 9.469262295081968 1102 14-15 8.328551912568308 1102 16-17 6.751366120218581 1102 18-19 12.599043715846992 1102 20-21 10.048497267759561 1102 22-23 14.87704918032787 1102 24-25 6.149590163934427 1102 26-27 14.934426229508198 1102 28-29 16.74658469945355 1102 30-31 16.323770491803273 1102 32-33 12.532786885245901 1102 34-35 13.189890710382514 1102 36-37 11.30464480874317 1102 38-39 17.94808743169399 1102 40-41 15.562841530054648 1102 42-43 19.1646174863388 1102 44-45 12.415983606557377 1102 46-47 15.896857923497269 1102 48-49 17.59016393442623 1102 50-51 16.481557377049178 1102 52-53 15.634562841530053 1102 54-55 18.18237704918033 1102 56-57 19.32786885245902 1102 58-59 19.562158469945352 1102 60-61 13.62704918032787 1102 62-63 4.27390710382514 1102 64-65 5.062158469945356 1102 66-67 3.3941256830601123 1102 68-69 5.562841530054646 1102 70-71 1.446721311475411 1102 72-73 2.7670765027322393 1102 74-75 3.9193989071038278 1102 76-77 6.469945355191255 1102 78-79 6.837431693989069 1102 80-81 7.325136612021858 1102 82-83 7.200136612021858 1102 84-85 6.878415300546449 1102 86-87 7.301229508196723 1102 88-89 4.853142076502731 1102 90-91 4.0150273224043715 1102 92-93 7.051912568306012 1102 94-95 2.4248633879781423 1102 96-97 4.403688524590162 1102 98-99 5.872267759562842 1102 100 7.004098360655737 1103 1 -14.387978142076499 1103 2 -14.30464480874317 1103 3 -14.663934426229506 1103 4 -17.393442622950822 1103 5 -16.40983606557377 1103 6 -16.602459016393443 1103 7 -16.65710382513661 1103 8 -16.769125683060107 1103 9 -17.814207650273225 1103 10-11 -17.56489071038251 1103 12-13 -17.28073770491803 1103 14-15 -17.421448087431692 1103 16-17 -16.24863387978142 1103 18-19 -16.400956284153008 1103 20-21 -15.951502732240439 1103 22-23 -16.12295081967213 1103 24-25 -14.850409836065573 1103 26-27 -14.815573770491802 1103 28-29 -14.75341530054645 1103 30-31 -14.426229508196727 1103 32-33 -13.967213114754099 1103 34-35 -12.560109289617486 1103 36-37 -13.19535519125683 1103 38-39 -13.301912568306012 1103 40-41 -13.187158469945352 1103 42-43 -12.585382513661203 1103 44-45 -12.584016393442623 1103 46-47 -12.353142076502731 1103 48-49 -11.659836065573773 1103 50-51 -11.768442622950822 1103 52-53 -12.365437158469947 1103 54-55 -11.81762295081967 1103 56-57 -11.922131147540982 1103 58-59 -11.437841530054646 1103 60-61 -11.12295081967213 1103 62-63 -11.22609289617486 1103 64-65 -11.187841530054644 1103 66-67 -11.105874316939888 1103 68-69 -10.687158469945354 1103 70-71 -10.303278688524589 1103 72-73 -9.73292349726776 1103 74-75 -9.580601092896172 1103 76-77 -9.530054644808745 1103 78-79 -9.16256830601093 1103 80-81 -8.674863387978142 1103 82-83 -8.549863387978142 1103 84-85 -8.621584699453551 1103 86-87 -8.198770491803277 1103 88-89 -8.396857923497269 1103 90-91 -8.234972677595628 1103 92-93 -7.948087431693988 1103 94-95 -7.325136612021858 1103 96-97 -7.346311475409838 1103 98-99 -7.627732240437158 1103 100 -7.495901639344263 1104 1 0.1120218579235015 1104 2 0.1953551912568301 1104 3 -0.16393442622950616 1104 4 -0.8934426229508219 1104 5 0.09016393442622928 1104 6 -0.10245901639344268 1104 7 -0.15710382513660903 1104 8 0.730874316939893 1104 9 0.6857923497267748 1104 10-11 0.9351092896174897 1104 12-13 -0.28073770491803174 1104 14-15 1.328551912568308 1104 16-17 0.0013661202185808463 1104 18-19 1.0990437158469923 1104 20-21 2.7984972677595614 1104 22-23 2.8770491803278695 1104 24-25 3.649590163934427 1104 26-27 1.6844262295081975 1104 28-29 1.7465846994535497 1104 30-31 1.3237704918032733 1104 32-33 4.032786885245901 1104 34-35 5.939890710382514 1104 36-37 5.05464480874317 1104 38-39 4.948087431693988 1104 40-41 5.062841530054648 1104 42-43 5.414617486338797 1104 44-45 4.915983606557377 1104 46-47 6.646857923497269 1104 48-49 7.8401639344262275 1104 50-51 4.481557377049178 1104 52-53 5.8845628415300535 1104 54-55 5.68237704918033 1104 56-57 7.077868852459018 1104 58-59 8.062158469945354 1104 60-61 8.12704918032787 1104 62-63 7.52390710382514 1104 64-65 7.812158469945356 1104 66-67 7.394125683060112 1104 68-69 7.812841530054646 1104 70-71 7.196721311475411 1104 72-73 8.01707650273224 1104 74-75 8.419398907103828 1104 76-77 7.219945355191255 1104 78-79 8.33743169398907 1104 80-81 8.325136612021858 1104 82-83 8.450136612021858 1104 84-85 8.378415300546449 1104 86-87 8.301229508196723 1104 88-89 6.603142076502731 1104 90-91 6.0150273224043715 1104 92-93 6.051912568306012 1104 94-95 6.924863387978142 1104 96-97 7.653688524590162 1104 98-99 8.372267759562842 1104 100 7.504098360655737 1105 1 -0.3879781420764985 1105 2 -0.3046448087431699 1105 3 -0.6639344262295062 1105 4 -0.8934426229508219 1105 5 -1.4098360655737707 1105 6 -1.1024590163934427 1105 7 -4.657103825136609 1105 8 -2.769125683060107 1105 9 -1.3142076502732252 1105 10-11 -1.8148907103825103 1105 12-13 -1.5307377049180317 1105 14-15 -5.421448087431692 1105 16-17 -6.748633879781419 1105 18-19 -5.400956284153008 1105 20-21 -8.201502732240439 1105 22-23 -2.8729508196721305 1105 24-25 0.8995901639344268 1105 26-27 2.1844262295081975 1105 28-29 -5.50341530054645 1105 30-31 -1.6762295081967267 1105 32-33 1.2827868852459012 1105 34-35 3.939890710382514 1105 36-37 3.55464480874317 1105 38-39 3.4480874316939882 1105 40-41 2.062841530054648 1105 42-43 3.9146174863387966 1105 44-45 0.16598360655737743 1105 46-47 -10.353142076502731 1105 48-49 -1.6598360655737725 1105 50-51 4.731557377049178 1105 52-53 -0.8654371584699465 1105 54-55 2.43237704918033 1105 56-57 0.5778688524590176 1105 58-59 1.812158469945354 1105 60-61 1.3770491803278695 1105 62-63 3.27390710382514 1105 64-65 4.312158469945356 1105 66-67 -0.3558743169398877 1105 68-69 4.312841530054646 1105 70-71 -1.553278688524589 1105 72-73 0.7670765027322393 1105 74-75 4.669398907103828 1105 76-77 4.719945355191255 1105 78-79 5.837431693989069 1105 80-81 -8.674863387978142 1105 82-83 -8.549863387978142 1105 84-85 -8.621584699453551 1105 86-87 -8.198770491803277 1105 88-89 -8.396857923497269 1105 90-91 -8.234972677595628 1105 92-93 -7.948087431693988 1105 94-95 -7.325136612021858 1105 96-97 -7.346311475409838 1105 98-99 -7.627732240437158 1105 100 -7.495901639344263 1106 1 4.945355191256834 1106 2 5.69535519125683 1106 3 4.669398907103826 1106 4 4.606557377049178 1106 5 4.923497267759561 1106 6 -2.9357923497267766 1106 7 2.342896174863391 1106 8 3.897540983606561 1106 9 1.519125683060107 1106 10-11 3.601775956284154 1106 12-13 6.0525956284153 1106 14-15 6.578551912568308 1106 16-17 6.751366120218581 1106 18-19 5.599043715846992 1106 20-21 5.548497267759561 1106 22-23 7.043715846994534 1106 24-25 6.816256830601091 1106 26-27 4.1844262295081975 1106 28-29 6.24658469945355 1106 30-31 0.5737704918032733 1106 32-33 3.699453551912569 1106 34-35 7.773224043715846 1106 36-37 2.80464480874317 1106 38-39 8.698087431693988 1106 40-41 8.312841530054648 1106 42-43 8.08128415300546 1106 44-45 11.249316939890713 1106 46-47 5.980191256830604 1106 48-49 7.006830601092892 1106 50-51 8.398224043715846 1106 52-53 7.967896174863389 1106 54-55 8.349043715846994 1106 56-57 7.077868852459018 1106 58-59 7.562158469945354 1106 60-61 8.210382513661202 1106 62-63 8.77390710382514 1106 64-65 8.145491803278691 1106 66-67 3.8941256830601123 1106 68-69 3.4795081967213104 1106 70-71 8.863387978142079 1106 72-73 5.100409836065575 1106 74-75 0.41939890710382777 1106 76-77 6.136612021857923 1106 78-79 10.33743169398907 1106 80-81 -6.008196721311476 1106 82-83 -5.883196721311476 1106 84-85 -2.4549180327868836 1106 86-87 -2.032103825136611 1106 88-89 0.43647540983606525 1106 90-91 2.2650273224043715 1106 92-93 0.051912568306011764 1106 94-95 -2.4918032786885247 1106 96-97 -7.346311475409838 1106 98-99 -7.627732240437158 1106 100 -7.495901639344263 1107 1 -6.3879781420764985 1107 2 -1.6379781420765038 1107 3 -6.663934426229506 1107 4 1.273224043715846 1107 5 -1.4098360655737707 1107 6 -6.935792349726777 1107 7 -1.657103825136609 1107 8 0.8975409836065609 1107 9 0.519125683060107 1107 10-11 -8.231557377049176 1107 12-13 -6.4474043715847 1107 14-15 -1.754781420765024 1107 16-17 -2.0819672131147513 1107 18-19 0.932377049180328 1107 20-21 -0.2848360655737707 1107 22-23 -0.7896174863387984 1107 24-25 0.6495901639344268 1107 26-27 3.6844262295081975 1107 28-29 2.913251366120214 1107 30-31 -6.259562841530059 1107 32-33 -7.967213114754099 1107 34-35 -8.89344262295082 1107 36-37 -7.528688524590164 1107 38-39 -4.801912568306012 1107 40-41 -10.353825136612018 1107 42-43 -4.918715846994537 1107 44-45 -3.5840163934426226 1107 46-47 -3.0198087431693974 1107 48-49 -11.659836065573773 1107 50-51 -11.768442622950822 1107 52-53 -12.365437158469947 1107 54-55 -11.81762295081967 1107 56-57 -11.922131147540982 1107 58-59 -11.437841530054646 1107 60-61 -11.12295081967213 1107 62-63 -11.22609289617486 1107 64-65 -11.187841530054644 1107 66-67 -11.105874316939888 1107 68-69 -10.687158469945354 1107 70-71 -10.303278688524589 1107 72-73 -9.73292349726776 1107 74-75 -9.580601092896172 1107 76-77 -9.530054644808745 1107 78-79 -9.16256830601093 1107 80-81 -8.674863387978142 1107 82-83 -8.549863387978142 1107 84-85 -8.621584699453551 1107 86-87 -8.198770491803277 1107 88-89 -8.396857923497269 1107 90-91 -8.234972677595628 1107 92-93 -7.948087431693988 1107 94-95 -7.325136612021858 1107 96-97 -7.346311475409838 1107 98-99 -7.627732240437158 1107 100 -7.495901639344263 1108 1 -0.3879781420764985 1108 2 -1.30464480874317 1108 3 -0.6639344262295062 1108 4 -0.8934426229508219 1108 5 -3.4098360655737707 1108 6 -0.10245901639344268 1108 7 -0.15710382513660903 1108 8 -4.769125683060107 1108 9 -1.3142076502732252 1108 10-11 0.9351092896174897 1108 12-13 0.21926229508196826 1108 14-15 -0.42144808743169193 1108 16-17 2.251366120218581 1108 18-19 -2.6509562841530077 1108 20-21 0.7984972677595614 1108 22-23 -0.1229508196721305 1108 24-25 1.8995901639344268 1108 26-27 2.6844262295081975 1108 28-29 2.2465846994535497 1108 30-31 3.3237704918032733 1108 32-33 3.0327868852459012 1108 34-35 4.439890710382514 1108 36-37 2.80464480874317 1108 38-39 2.6980874316939882 1108 40-41 0.8128415300546479 1108 42-43 2.9146174863387966 1108 44-45 3.4159836065573774 1108 46-47 0.8968579234972687 1108 48-49 0.5901639344262275 1108 50-51 3.481557377049178 1108 52-53 2.3845628415300535 1108 54-55 2.93237704918033 1108 56-57 -2.9221311475409824 1108 58-59 -0.4378415300546461 1108 60-61 1.1270491803278695 1108 62-63 0.27390710382513994 1108 64-65 -0.18784153005464432 1108 66-67 3.6441256830601123 1108 68-69 4.812841530054646 1108 70-71 3.946721311475411 1108 72-73 5.767076502732239 1108 74-75 4.419398907103828 1108 76-77 4.219945355191255 1108 78-79 4.087431693989069 1108 80-81 1.8251366120218577 1108 82-83 6.450136612021858 1108 84-85 3.8784153005464486 1108 86-87 7.301229508196723 1108 88-89 5.603142076502731 1108 90-91 6.7650273224043715 1108 92-93 3.5519125683060118 1108 94-95 7.174863387978142 1108 96-97 8.153688524590162 1108 98-99 7.872267759562842 1108 100 7.004098360655737 1109 1 1.6120218579235015 1109 2 11.19535519125683 1109 3 7.836065573770494 1109 4 5.606557377049178 1109 5 -2.4098360655737707 1109 6 3.3975409836065573 1109 7 2.842896174863391 1109 8 7.230874316939893 1109 9 14.685792349726775 1109 10-11 5.93510928961749 1109 12-13 8.219262295081968 1109 14-15 14.078551912568308 1109 16-17 9.00136612021858 1109 18-19 12.849043715846992 1109 20-21 6.298497267759561 1109 22-23 9.62704918032787 1109 24-25 6.899590163934427 1109 26-27 5.1844262295081975 1109 28-29 -0.25341530054645034 1109 30-31 8.323770491803273 1109 32-33 1.5327868852459012 1109 34-35 -8.560109289617486 1109 36-37 -2.19535519125683 1109 38-39 1.6980874316939882 1109 40-41 -1.4371584699453521 1109 42-43 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6.93989071038251 2211 5 3.256830601092897 2211 6 9.064207650273225 2211 7 13.676229508196727 2211 8 3.564207650273225 2211 9 5.852459016393443 2211 10-11 10.101775956284154 2211 12-13 3.7192622950819683 2211 14-15 0.9118852459016402 2211 16-17 0.08469945355191655 2211 18-19 -3.4009562841530077 2211 20-21 3.381830601092897 2211 22-23 2.210382513661205 2211 24-25 1.816256830601091 2211 26-27 2.0177595628415332 2211 28-29 6.9132513661202175 2211 30-31 1.407103825136609 2211 32-33 6.6994535519125655 2211 34-35 -4.226775956284152 2211 36-37 -4.69535519125683 2211 38-39 -7.468579234972678 2211 40-41 -3.0204918032786843 2211 42-43 -1.4187158469945356 2211 44-45 -5.750683060109289 2211 46-47 -2.5198087431693974 2211 48-49 -1.8265027322404404 2211 50-51 -6.101775956284155 2211 52-53 -9.865437158469947 2211 54-55 -2.150956284153004 2211 56-57 -1.0887978142076502 2211 58-59 -2.2711748633879782 2211 60-61 -1.9562841530054627 2211 62-63 -2.059426229508192 2211 64-65 -2.354508196721312 2211 66-67 -2.43920765027322 2211 68-69 -10.687158469945354 2211 70-71 -10.303278688524589 2211 72-73 -9.73292349726776 2211 74-75 -9.580601092896172 2211 76-77 -9.530054644808745 2211 78-79 -9.16256830601093 2211 80-81 -8.674863387978142 2211 82-83 -8.549863387978142 2211 84-85 -8.621584699453551 2211 86-87 -8.198770491803277 2211 88-89 -8.396857923497269 2211 90-91 -8.234972677595628 2211 92-93 -7.948087431693988 2211 94-95 -7.325136612021858 2211 96-97 -7.346311475409838 2211 98-99 -7.627732240437158 2211 100 -7.495901639344263 2212 1 -2.8879781420764985 2212 2 -2.80464480874317 2212 3 -2.163934426229506 2212 4 -7.143442622950822 2212 5 -2.9098360655737707 2212 6 -2.6024590163934427 2212 7 -1.407103825136609 2212 8 -1.019125683060107 2212 9 -0.8142076502732252 2212 10-11 -1.5648907103825103 2212 12-13 -2.4057377049180317 2212 14-15 -5.546448087431692 2212 16-17 -5.248633879781419 2212 18-19 -3.5259562841530077 2212 20-21 -1.9515027322404386 2212 22-23 -0.3729508196721305 2212 24-25 0.8995901639344268 2212 26-27 -1.9405737704918025 2212 28-29 -3.3784153005464503 2212 30-31 -3.3012295081967267 2212 32-33 0.6577868852459012 2212 34-35 0.3148907103825138 2212 36-37 2.17964480874317 2212 38-39 4.448087431693988 2212 40-41 -1.6871584699453521 2212 42-43 -0.08538251366120342 2212 44-45 -3.2090163934426226 2212 46-47 -7.853142076502731 2212 48-49 -0.4098360655737725 2212 50-51 -2.893442622950822 2212 52-53 -2.8654371584699465 2212 54-55 -4.94262295081967 2212 56-57 -1.9221311475409824 2212 58-59 -1.437841530054646 2212 60-61 0.2520491803278695 2212 62-63 -0.47609289617486006 2212 64-65 0.4371584699453557 2212 66-67 0.7691256830601123 2212 68-69 0.4378415300546461 2212 70-71 4.321721311475411 2212 72-73 0.39207650273223926 2212 74-75 -6.080601092896172 2212 76-77 -9.530054644808745 2212 78-79 -9.16256830601093 2212 80-81 -8.674863387978142 2212 82-83 -8.549863387978142 2212 84-85 -8.621584699453551 2212 86-87 -8.198770491803277 2212 88-89 -8.396857923497269 2212 90-91 -8.234972677595628 2212 92-93 -7.948087431693988 2212 94-95 -7.325136612021858 2212 96-97 -7.346311475409838 2212 98-99 -7.627732240437158 2212 100 -7.495901639344263 2213 1 2.2786885245901694 2213 2 -2.971311475409836 2213 3 2.336065573770494 2213 4 -1.726775956284154 2213 5 2.9234972677595614 2213 6 -4.602459016393443 2213 7 -0.657103825136609 2213 8 -0.43579234972677483 2213 9 -1.8142076502732252 2213 10-11 1.7684426229508254 2213 12-13 3.3859289617486326 2213 14-15 -2.0881147540983562 2213 16-17 -5.748633879781419 2213 18-19 -5.23428961748634 2213 20-21 -5.2848360655737725 2213 22-23 -4.6229508196721305 2213 24-25 -4.017076502732241 2213 26-27 -4.8155737704918025 2213 28-29 -4.420081967213116 2213 30-31 -4.759562841530059 2213 32-33 -1.800546448087431 2213 34-35 -0.060109289617486183 2213 36-37 -0.862021857923498 2213 38-39 -0.9685792349726796 2213 40-41 -0.520491803278686 2213 42-43 -0.9187158469945373 2213 44-45 -1.4173497267759547 2213 46-47 -5.1864754098360635 2213 48-49 -2.8265027322404386 2213 50-51 0.06489071038251026 2213 52-53 -1.0321038251366144 2213 54-55 -0.48428961748633625 2213 56-57 -2.7554644808743163 2213 58-59 -0.27117486338798 2213 60-61 -3.9562841530054627 2213 62-63 -2.059426229508194 2213 64-65 -1.5211748633879782 2213 66-67 -2.4392076502732216 2213 68-69 -2.020491803278688 2213 70-71 0.19672131147541094 2213 72-73 0.9337431693989071 2213 74-75 1.0860655737704956 2213 76-77 -0.5300546448087449 2213 78-79 0.33743169398906936 2213 80-81 1.4918032786885238 2213 82-83 0.7834699453551899 2213 84-85 0.5450819672131164 2213 86-87 -1.032103825136609 2213 88-89 1.4364754098360635 2213 90-91 1.7650273224043715 2213 92-93 2.218579234972678 2213 94-95 3.1748633879781423 2213 96-97 2.9870218579234944 2213 98-99 3.038934426229508 2213 100 3.1707650273224033 2214 1 -3.7213114754098324 2214 2 -4.637978142076504 2214 3 -4.99726775956284 2214 4 -5.726775956284156 2214 5 -4.743169398907105 2214 6 -4.935792349726777 2214 7 -4.990437158469943 2214 8 -5.102459016393441 2214 9 -5.814207650273225 2214 10-11 -5.231557377049176 2214 12-13 -5.4474043715847 2214 14-15 -5.08811475409836 2214 16-17 -3.9153005464480852 2214 18-19 -4.23428961748634 2214 20-21 -3.4515027322404386 2214 22-23 -3.6229508196721305 2214 24-25 -2.350409836065573 2214 26-27 -3.3155737704918025 2214 28-29 -2.2534153005464503 2214 30-31 -1.9262295081967267 2214 32-33 -1.9672131147540988 2214 34-35 -0.22677595628415226 2214 36-37 -1.19535519125683 2214 38-39 -0.4685792349726796 2214 40-41 -0.35382513661202 2214 42-43 0.4146174863387966 2214 44-45 0.4159836065573774 2214 46-47 0.14685792349726867 2214 48-49 1.3401639344262275 2214 50-51 1.0648907103825103 2214 52-53 -0.19877049180327866 2214 54-55 0.8490437158469959 2214 56-57 0.7445355191256837 2214 58-59 1.395491803278686 2214 60-61 1.2103825136612016 2214 62-63 1.107240437158472 2214 64-65 1.1454918032786878 2214 66-67 1.0607923497267802 2214 68-69 2.1461748633879782 2214 70-71 2.363387978142077 2214 72-73 3.1004098360655714 2214 74-75 2.4193989071038278 2214 76-77 2.803278688524589 2214 78-79 2.5040983606557354 2214 80-81 1.6584699453551917 2214 82-83 1.4501366120218577 2214 84-85 2.0450819672131146 2214 86-87 1.4678961748633892 2214 88-89 1.6031420765027313 2214 90-91 2.4316939890710376 2214 92-93 2.385245901639344 2214 94-95 3.3415300546448083 2214 96-97 3.4870218579234944 2214 98-99 3.372267759562842 2214 100 3.504098360655737 2215 1 -8.637978142076499 2215 2 -7.30464480874317 2215 3 -7.413934426229506 2215 4 -9.143442622950822 2215 5 -10.65983606557377 2215 6 -8.352459016393443 2215 7 -8.907103825136609 2215 8 -8.519125683060107 2215 9 -10.064207650273225 2215 10-11 -10.81489071038251 2215 12-13 -10.030737704918032 2215 14-15 -10.171448087431692 2215 16-17 -9.62363387978142 2215 18-19 -8.400956284153008 2215 20-21 -6.576502732240439 2215 22-23 -7.4979508196721305 2215 24-25 -6.100409836065573 2215 26-27 -7.4405737704918025 2215 28-29 -8.25341530054645 2215 30-31 -10.301229508196727 2215 32-33 -6.467213114754099 2215 34-35 -4.435109289617486 2215 36-37 -4.69535519125683 2215 38-39 -7.676912568306012 2215 40-41 -9.687158469945352 2215 42-43 -7.335382513661203 2215 44-45 -4.584016393442623 2215 46-47 -4.603142076502731 2215 48-49 -3.9098360655737725 2215 50-51 -3.143442622950822 2215 52-53 -4.9904371584699465 2215 54-55 -3.56762295081967 2215 56-57 -2.7971311475409824 2215 58-59 -2.812841530054646 2215 60-61 -4.9979508196721305 2215 62-63 -2.97609289617486 2215 64-65 -5.562841530054644 2215 66-67 -5.230874316939888 2215 68-69 -4.687158469945354 2215 70-71 -3.678278688524589 2215 72-73 -6.482923497267761 2215 74-75 -4.580601092896172 2215 76-77 -2.905054644808745 2215 78-79 -0.03756830601093064 2215 80-81 -0.29986338797814227 2215 82-83 -3.0498633879781423 2215 84-85 -3.2465846994535514 2215 86-87 -1.6987704918032769 2215 88-89 -0.8968579234972687 2215 90-91 -2.2349726775956285 2215 92-93 -1.0730874316939882 2215 94-95 0.42486338797814227 2215 96-97 -1.0963114754098378 2215 98-99 -1.2527322404371581 2215 100 -0.7459016393442628 2216 1 10.612021857923501 2216 2 11.69535519125683 2216 3 14.336065573770494 2216 4 15.606557377049178 2216 5 13.59016393442623 2216 6 16.397540983606557 2216 7 11.342896174863391 2216 8 13.230874316939893 2216 9 17.185792349726775 2216 10-11 17.43510928961749 2216 12-13 14.719262295081968 2216 14-15 19.078551912568308 2216 16-17 18.25136612021858 2216 18-19 8.099043715846992 2216 20-21 19.04849726775956 2216 22-23 19.87704918032787 2216 24-25 20.149590163934427 2216 26-27 20.184426229508198 2216 28-29 19.24658469945355 2216 30-31 19.073770491803273 2216 32-33 21.0327868852459 2216 34-35 19.439890710382514 2216 36-37 23.30464480874317 2216 38-39 21.69808743169399 2216 40-41 24.312841530054648 2216 42-43 24.4146174863388 2216 44-45 22.915983606557376 2216 46-47 21.14685792349727 2216 48-49 19.34016393442623 2216 50-51 19.231557377049178 2216 52-53 21.134562841530055 2216 54-55 6.68237704918033 2216 56-57 15.577868852459018 2216 58-59 24.062158469945352 2216 60-61 24.87704918032787 2216 62-63 21.773907103825138 2216 64-65 19.312158469945356 2216 66-67 22.89412568306011 2216 68-69 22.312841530054648 2216 70-71 18.19672131147541 2216 72-73 22.267076502732237 2216 74-75 22.919398907103826 2216 76-77 22.969945355191257 2216 78-79 20.337431693989068 2216 80-81 18.325136612021858 2216 82-83 17.950136612021858 2216 84-85 17.37841530054645 2216 86-87 20.801229508196723 2216 88-89 24.10314207650273 2216 90-91 22.76502732240437 2216 92-93 24.05191256830601 2216 94-95 15.674863387978142 2216 96-97 19.653688524590162 2216 98-99 16.372267759562842 2216 100 21.50409836065574 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 564.0 3 27.0 4 23.0 5 25.0 6 24.0 7 32.0 8 25.0 9 23.0 10 28.0 11 26.0 12 21.0 13 12.0 14 17.0 15 13.0 16 13.0 17 10.0 18 14.0 19 12.0 20 11.0 21 12.0 22 15.0 23 18.0 24 21.0 25 16.0 26 17.0 27 21.0 28 22.0 29 36.0 30 30.0 31 31.0 32 38.0 33 33.0 34 43.0 35 46.0 36 63.0 37 52.0 38 37.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.769647696476966 18.97018970189702 15.514905149051492 25.745257452574528 2 16.677966101694917 22.305084745762713 34.779661016949156 26.23728813559322 3 21.83050847457627 24.610169491525426 27.05084745762712 26.508474576271183 4 17.28813559322034 18.64406779661017 30.16949152542373 33.89830508474576 5 16.406779661016948 34.37288135593221 33.08474576271186 16.135593220338983 6 32.13559322033898 36.20338983050848 14.847457627118644 16.8135593220339 7 27.168021680216803 31.70731707317073 19.986449864498645 21.138211382113823 8 27.77777777777778 36.11111111111111 16.056910569105693 20.05420054200542 9 28.252032520325205 14.70189701897019 20.32520325203252 36.72086720867209 10-11 24.712254570074474 25.152335815842925 26.844955991875423 23.290453622207178 12-13 25.262444971215714 23.39993227226549 26.98950220115137 24.348120555367423 14-15 24.847664184157075 25.490859851049425 23.662830060934326 25.998645903859174 16-17 25.829383886255926 27.488151658767773 23.019634394041976 23.662830060934326 18-19 25.25389302640487 28.165199729180774 24.373730534867978 22.207176709546378 20-21 24.542992552471226 26.43872714962762 25.118483412322274 23.899796885578876 22-23 24.771263978312437 26.838359878007456 25.042358522534734 23.348017621145374 24-25 24.796747967479675 26.321138211382113 23.712737127371277 25.16937669376694 26-27 25.770402980020318 25.093125634947512 23.433796139519135 25.702675245513042 28-29 26.675693974272175 25.287745429925522 23.595125253893027 24.441435341909276 30-31 24.15311653116531 25.914634146341463 24.1869918699187 25.745257452574528 32-33 25.982384823848236 23.78048780487805 23.712737127371277 26.52439024390244 34-35 28.53757616790792 24.102911306702776 22.308733920108327 25.050778605280975 36-37 26.844955991875423 22.545700744752878 22.951929587000677 27.65741367637102 38-39 26.303317535545023 24.576844955991874 21.39471902505078 27.72511848341232 40-41 24.373730534867978 25.321597833446173 24.915368991198374 25.389302640487475 42-43 24.288617886178862 25.203252032520325 24.728997289972902 25.779132791327914 44-45 25.57588075880759 24.83062330623306 25.44037940379404 24.15311653116531 46-47 24.4579945799458 25.914634146341463 23.170731707317074 26.456639566395662 48-49 26.0 24.779661016949152 23.864406779661017 25.35593220338983 50-51 25.83050847457627 25.186440677966104 24.610169491525426 24.3728813559322 52-53 26.47457627118644 25.457627118644066 23.69491525423729 24.3728813559322 54-55 25.35593220338983 26.813559322033896 22.71186440677966 25.11864406779661 56-57 24.54237288135593 25.186440677966104 24.3728813559322 25.898305084745765 58-59 27.83050847457627 24.305084745762713 23.25423728813559 24.610169491525426 60-61 27.559322033898304 24.47457627118644 23.220338983050848 24.74576271186441 62-63 28.54237288135593 25.11864406779661 22.033898305084744 24.305084745762713 64-65 26.644067796610173 24.203389830508474 23.69491525423729 25.457627118644066 66-67 25.593220338983052 25.08474576271186 22.237288135593218 27.084745762711865 68-69 27.75330396475771 24.296848525923416 24.296848525923416 23.65299898339546 70-71 25.982384823848236 24.424119241192415 23.78048780487805 25.8130081300813 72-73 27.33739837398374 24.695121951219512 23.678861788617887 24.288617886178862 74-75 29.437669376693766 23.340108401084013 22.79810298102981 24.424119241192415 76-77 27.40514905149051 24.55962059620596 24.35636856368564 23.678861788617887 78-79 28.01490514905149 24.4579945799458 23.577235772357724 23.94986449864499 80-81 25.855642155201625 26.363944425618435 22.56862080650627 25.211792612673673 82-83 29.539295392953928 23.577235772357724 21.951219512195124 24.932249322493224 84-85 27.497460209955975 23.603115475787334 23.46766000677277 25.431764307483917 86-87 27.269647696476966 24.119241192411923 22.289972899728998 26.321138211382113 88-89 27.65741367637102 24.306025727826675 21.36086662153013 26.675693974272175 90-91 27.89438050101557 23.22274881516588 25.423155044008123 23.459715639810426 92-93 26.709546377792826 24.441435341909276 25.389302640487475 23.459715639810426 94-95 27.048070412999326 23.628977657413678 24.069058903182125 25.25389302640487 96-97 26.844955991875423 22.78266756939743 23.561272850372376 26.811103588354772 98-99 26.980365605958024 24.102911306702776 25.355450236966824 23.561272850372376 100 27.623561272850374 23.019634394041976 24.441435341909276 24.915368991198374 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 0.5 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 0.5 21 0.0 22 0.0 23 2.0 24 4.0 25 5.0 26 3.5 27 4.5 28 8.5 29 9.5 30 9.5 31 11.5 32 16.5 33 19.5 34 21.0 35 26.0 36 30.0 37 32.0 38 30.5 39 26.0 40 27.0 41 38.0 42 40.5 43 32.5 44 35.0 45 48.0 46 53.0 47 46.5 48 48.0 49 47.5 50 39.5 51 41.0 52 46.5 53 44.0 54 40.5 55 43.5 56 42.5 57 37.0 58 37.0 59 35.5 60 38.0 61 34.0 62 28.0 63 27.0 64 20.5 65 21.0 66 24.0 67 19.0 68 14.0 69 17.0 70 20.0 71 16.5 72 11.0 73 11.0 74 10.5 75 9.5 76 10.5 77 8.0 78 5.0 79 4.5 80 5.0 81 7.0 82 7.5 83 5.0 84 3.0 85 2.5 86 3.0 87 2.0 88 0.5 89 0.5 90 1.5 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13531799729364005 2 0.20297699594046006 3 0.20297699594046006 4 0.20297699594046006 5 0.20297699594046006 6 0.20297699594046006 7 0.13531799729364005 8 0.13531799729364005 9 0.13531799729364005 10-11 0.06765899864682003 12-13 0.10148849797023003 14-15 0.06765899864682003 16-17 0.06765899864682003 18-19 0.06765899864682003 20-21 0.06765899864682003 22-23 0.16914749661705006 24-25 0.13531799729364005 26-27 0.10148849797023003 28-29 0.06765899864682003 30-31 0.13531799729364005 32-33 0.13531799729364005 34-35 0.06765899864682003 36-37 0.06765899864682003 38-39 0.06765899864682003 40-41 0.06765899864682003 42-43 0.13531799729364005 44-45 0.13531799729364005 46-47 0.13531799729364005 48-49 0.20297699594046006 50-51 0.20297699594046006 52-53 0.20297699594046006 54-55 0.20297699594046006 56-57 0.20297699594046006 58-59 0.20297699594046006 60-61 0.20297699594046006 62-63 0.20297699594046006 64-65 0.20297699594046006 66-67 0.20297699594046006 68-69 0.16914749661705006 70-71 0.13531799729364005 72-73 0.13531799729364005 74-75 0.13531799729364005 76-77 0.13531799729364005 78-79 0.13531799729364005 80-81 0.16914749661705006 82-83 0.13531799729364005 84-85 0.10148849797023003 86-87 0.13531799729364005 88-89 0.06765899864682003 90-91 0.06765899864682003 92-93 0.06765899864682003 94-95 0.06765899864682003 96-97 0.06765899864682003 98-99 0.06765899864682003 100 0.06765899864682003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1478.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.03382949932341001 0.0 38-39 0.0 0.0 0.0 0.13531799729364005 0.0 40-41 0.0 0.0 0.0 0.13531799729364005 0.0 42-43 0.0 0.0 0.0 0.3044654939106901 0.0 44-45 0.0 0.0 0.0 0.4059539918809202 0.0 46-47 0.0 0.0 0.0 0.4736129905277402 0.0 48-49 0.0 0.0 0.0 0.4736129905277402 0.0 50-51 0.0 0.0 0.0 0.5074424898511503 0.0 52-53 0.0 0.0 0.0 0.5412719891745602 0.0 54-55 0.0 0.0 0.0 0.7780784844384303 0.0 56-57 0.0 0.0 0.0 0.9133964817320703 0.0 58-59 0.0 0.0 0.0 0.9472259810554804 0.0 60-61 0.0 0.0 0.0 0.9810554803788905 0.0 62-63 0.0 0.0 0.0 1.0487144790257106 0.0 64-65 0.0 0.0 0.0 1.0825439783491204 0.0 66-67 0.0 0.0 0.0 1.2178619756427604 0.0 68-69 0.0 0.0 0.0 1.5561569688768606 0.0 70-71 0.0 0.0 0.0 1.6914749661705009 0.0 72-73 0.0 0.0 0.0 1.7929634641407306 0.0 74-75 0.0 0.0 0.0 1.995940460081191 0.0 76-77 0.0 0.0 0.0 2.3004059539918806 0.0 78-79 0.0 0.0 0.0 2.7063599458728014 0.0 80-81 0.0 0.0 0.0 2.943166441136671 0.0 82-83 0.0 0.0 0.0 3.2138024357239514 0.0 84-85 0.0 0.0 0.0 3.4506089309878214 0.0 86-87 0.0 0.0 0.0 3.6197564276048713 0.0 88 0.0 0.0 0.0 3.9918809201623815 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE