FastQCFastQC Report
Wed 25 May 2016
SRR1032979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCG120.8119079837618403No Hit
GTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATC90.6089309878213802No Hit
TGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACT90.6089309878213802No Hit
GTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTT70.4736129905277402No Hit
CCCCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTC70.4736129905277402No Hit
CGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGC70.4736129905277402No Hit
ATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAA50.3382949932341001No Hit
CCTCTACCGCATGCGAATCTTTGCGCCTAATCATGTCGTCGCCAAGTCCC50.3382949932341001No Hit
GTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTA50.3382949932341001No Hit
CAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCA50.3382949932341001No Hit
GATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGA40.2706359945872801No Hit
CTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTG40.2706359945872801No Hit
GAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCAT30.20297699594046006No Hit
ATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAA30.20297699594046006No Hit
GGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCC30.20297699594046006No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA30.20297699594046006RNA PCR Primer, Index 23 (96% over 30bp)
CTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGG30.20297699594046006No Hit
AGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAG30.20297699594046006No Hit
GTACCTGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA30.20297699594046006No Hit
CTCCAGGACAGTTCAGGTCCCCAAGCTAACCCCCTACTTCCCAGACAGCT30.20297699594046006No Hit
GGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGT30.20297699594046006No Hit
GTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGA30.20297699594046006No Hit
CTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCA30.20297699594046006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20297699594046006No Hit
GTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGC30.20297699594046006No Hit
ATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGA30.20297699594046006No Hit
CCCACAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTC30.20297699594046006No Hit
GTCCCGCTTCTGGTACTTTGTATCTCAGTTAAAGAAGATGAAGAAGTCTT30.20297699594046006No Hit
TTGTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTAC30.20297699594046006No Hit
GTATCTCAGTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTG30.20297699594046006No Hit
CCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCCGGAGC30.20297699594046006No Hit
ATAATATCCCGGGGCAAACCGGAGAATTTGGCAGTCCGATTGGGGGGGGG30.20297699594046006No Hit
CCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAATCTTTGCGCCT30.20297699594046006No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG30.20297699594046006RNA PCR Primer, Index 23 (96% over 30bp)
GGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTG30.20297699594046006No Hit
CGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGGGAATACCGGGA20.13531799729364005No Hit
TCACAGCGCTGCGCCGCACAGTGCCCCCCGCTGTCACTGGGATCACCTTC20.13531799729364005No Hit
GTTAAAGAAGATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGG20.13531799729364005No Hit
ATTTATTATAGGCCAGTGATGTCTCAAAGAGTAGAGGAGCGTCTACTGGT20.13531799729364005No Hit
GAGTACATGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG20.13531799729364005No Hit
CAACATGTACCGGGAATACCGGGACCTGACCACCGCAGGCGCTGTCACCC20.13531799729364005No Hit
GAGCACGCCATGAAGGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGG20.13531799729364005No Hit
GGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCGGGATCTG20.13531799729364005No Hit
AGAGTACAAGGTAGTGGGTCGCTGCCTGCCCACCCCCAAATGCCACACGC20.13531799729364005No Hit
GGATCTGGCTGCGCTATGACTCCCGGAGCGGCACCCACAACATGTACCGG20.13531799729364005No Hit
TTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAAC20.13531799729364005No Hit
GTTTGTGGCCATGATTACCTTGACATTGACATTCTGATCAAATCCATCCA20.13531799729364005No Hit
TCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGATCT20.13531799729364005No Hit
CATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATCCTCCAGGAT20.13531799729364005No Hit
CTATAATATCCCGGGGCAAACCGGAGAATTTGGCAGTCCGATTGGGGGGG20.13531799729364005No Hit
GGCCTCGGGCACGCTACGAGAGTACAAGGTAGTGGGTCGCTGCCTGCCCA20.13531799729364005No Hit
GGTCACGCAGTCAGCCCAGGTCTGTGGTCGCTGCAGCACCAGGCTGCGCT20.13531799729364005No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA20.13531799729364005RNA PCR Primer, Index 26 (100% over 29bp)
TCTTGCAGGTGGCTCTTCCTCCACATCACGACTGGGGTCTCGAAGAGGAC20.13531799729364005No Hit
CTTCCTCCACATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAATC20.13531799729364005No Hit
CTCTTCCTCCACATCACGACTGGGGTCTCGAAGAGGACGGGGGTAGGAAA20.13531799729364005No Hit
CTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATGCGAA20.13531799729364005No Hit
GTACATGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAA20.13531799729364005No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG20.13531799729364005RNA PCR Primer, Index 31 (96% over 29bp)
CTCCTGCTCAATGGTCTCTTTGGTCGGCAGGGTGTTCTTCTCCTGCGTCT20.13531799729364005No Hit
ACGCAGAGTACATGGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCAC20.13531799729364005No Hit
GTGCAGCTTGTGGCTCGTGTCCATCTTGCAGGTGGCTCTTCCTCCACATC20.13531799729364005No Hit
GATGAAGAAGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGA20.13531799729364005No Hit
GTCCCCCCTGCGGGTGAAGAACTTCGGGATCTGGCTGCGCTATGACTCCC20.13531799729364005No Hit
GTCGCTGCCTGCCCACCCCCAAATGCCACACGCCGCCCCTCTACCGCATG20.13531799729364005No Hit
CTACTGTGGGCAGGTGTTTGAGAAGTCCCCCCTGCGGGTGAAGAACTTCG20.13531799729364005No Hit
ATGCAACACTGAACTGGGGAACATGGCTTGGGGCCTCCAGGACAGTTCAG20.13531799729364005No Hit
CATCTACAGACACGATCAGGCACGCAGCCTCAGAGGCTGCTGTCAGCGCA20.13531799729364005No Hit
CTGCAGAACTGGTTCAGTTCGTGGTGGATCTTGTTGAAGATGAGGGCCTT20.13531799729364005No Hit
AGTCTTCAGGGGAGATTGTCTACTGTGGGCAGGTGTTTGAGAAGTCCCCC20.13531799729364005No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)