FastQCFastQC Report
Wed 25 May 2016
SRR1032978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4444478
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62350.034.4500121
TCAACGC60700.034.1479344
ATCAACG62700.032.983353
GGTATCA38900.032.8646431
CAACGCA63650.032.637645
AACGCAG69150.030.2456426
GTACATG146400.028.5410731
TATCAAC76950.027.4861242
TACATGG147950.026.939562
ACATGGG148750.025.593973
ACGCAGA81500.025.0851557
CGCAGAG82750.024.6494318
TAGACCG1602.0411135E-623.4998425
CATGGGG96950.020.4587524
GAGTACA107950.019.1575361
GCAGAGT104000.019.1153569
GTATTAG24150.018.294491
GTACGAC3106.375558E-918.194043
AGTACAT106800.018.1315782
CATGGGA52000.017.2641224
Produced by FastQC (version 0.11.5)