FastQCFastQC Report
Wed 25 May 2016
SRR1032978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4444478
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75050.16886122509775053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62800.1412989331930544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68250.049.0132451
GTATCAA117000.040.758321
ATCAACG140350.033.182683
TCAACGC140750.033.0880054
CAACGCA143450.032.4280435
TATCAAC150450.031.1735822
AACGCAG150100.031.0540336
GTACATG146150.027.8069521
ACGCAGA174750.026.5650317
TACATGG149550.026.108112
CGCAGAG178150.025.926038
ACATGGG150900.024.8772453
GCAGAGT197300.023.2137459
CATGGGG101300.021.9652164
TAGACCG1954.6465357E-721.7086565
GTGGTAT32450.020.7041531
AGAGTAC182550.020.17423410-11
GAGTACT123950.019.74507912-13
CAGAGTA192500.019.7415210-11
CCGATCG2502.445213E-718.790049
Produced by FastQC (version 0.11.5)