FastQCFastQC Report
Wed 25 May 2016
SRR1032977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064847
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52870.49650325351905017No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16750.1572995932749024No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11110.1043342376886069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATC851.7543545E-533.1773152
CAGTGCG856.816613E-427.6464639
GTACATG43550.022.8800321
TACATGG43350.022.4434782
GCGGTAT1050.002335037622.3815231
CATGGGG18350.021.7716714
GTCGCCG3301.8189894E-1221.36317894
ACATGGG46650.019.8483093
GTATAGG2151.1935335E-619.674921
GTATATA7150.017.7487031
GCGCCGG1902.0230244E-417.31541894
CAACACA16850.017.0144716
GCTATAC1952.46608E-416.8722271
TCGCCGT1700.001279562716.58787794
GGGGCGC2607.32391E-616.26888394
GTATTAT6450.016.0314181
GTATCAA56300.015.9453441
CCTATAT3855.344191E-915.8705351
GCCGGAT1500.00635926915.6663394
ACAACAC10550.015.5920825
Produced by FastQC (version 0.11.5)