FastQCFastQC Report
Wed 25 May 2016
SRR1032977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064847
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53940.5065516454476559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51060.47950550642486667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26890.25252454108430605No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT25290.23749890829386755No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17530.16462458926024115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.12875089097306938No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10850.10189257236016065No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT10650.10001436826135586RNA PCR Primer, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC757.3879146E-637.6407853
GGTATCA60900.032.2549931
GTATCAA89950.029.5178851
TATAACG1005.283982E-528.2305872
CGGGTCA700.00880124426.8786896
ATCAACG103650.025.5114823
TATCAAC104100.025.4915982
TCAACGC103950.025.4378574
CAACGCA104350.025.3343895
AACGCAG107900.024.6304956
GAGTACT70900.023.35651612-13
CGCAGAG116500.022.6900838
ACGCAGA118600.022.329037
GCAGAGT120650.021.493499
AGTACTT73400.020.89692112-13
GTACTTT80250.020.66449514-15
AGAGTAC96600.020.1820910-11
GTATAGG2401.5818841E-719.5806311
GTACATG43600.019.5087781
CATGGGG16200.019.4593564
Produced by FastQC (version 0.11.5)