##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032977_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1064847 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.787058610298004 34.0 31.0 34.0 31.0 34.0 2 32.83367657513239 34.0 31.0 34.0 31.0 34.0 3 32.9059442342421 34.0 31.0 34.0 31.0 34.0 4 36.33584355311139 37.0 37.0 37.0 35.0 37.0 5 36.32483727709239 37.0 37.0 37.0 35.0 37.0 6 36.3913012855368 37.0 37.0 37.0 35.0 37.0 7 36.35749173355421 37.0 37.0 37.0 35.0 37.0 8 36.29937164681874 37.0 37.0 37.0 35.0 37.0 9 38.12875934289151 39.0 39.0 39.0 37.0 39.0 10-11 38.15284402360152 39.0 39.0 39.0 37.0 39.0 12-13 38.15060708251984 39.0 39.0 39.0 37.0 39.0 14-15 39.64655016166642 41.0 40.0 41.0 37.0 41.0 16-17 39.663723990394864 41.0 40.0 41.0 37.0 41.0 18-19 39.730223684717146 41.0 40.0 41.0 37.0 41.0 20-21 39.75443420510177 41.0 40.0 41.0 37.0 41.0 22-23 39.69422226855126 41.0 40.0 41.0 37.0 41.0 24-25 39.655142006316396 41.0 40.0 41.0 37.0 41.0 26-27 39.54182431842321 41.0 40.0 41.0 36.5 41.0 28-29 39.44146952566894 41.0 39.5 41.0 36.0 41.0 30-31 39.23890850047002 41.0 39.0 41.0 35.5 41.0 32-33 38.98756065425361 41.0 39.0 41.0 35.0 41.0 34-35 38.81987459231232 41.0 39.0 41.0 35.0 41.0 36-37 38.66681692299457 41.0 38.5 41.0 35.0 41.0 38-39 38.487067156126656 41.0 38.0 41.0 35.0 41.0 40-41 38.31761652143453 41.0 38.0 41.0 35.0 41.0 42-43 38.08400878248237 41.0 38.0 41.0 34.5 41.0 44-45 37.76625515214862 41.0 38.0 41.0 33.5 41.0 46-47 37.36501488007197 41.0 38.0 41.0 33.0 41.0 48-49 37.02353201915392 41.0 38.0 41.0 31.5 41.0 50-51 36.807765810487325 41.0 38.0 41.0 31.0 41.0 52-53 36.67293282509131 40.0 38.0 41.0 30.5 41.0 54-55 36.54090916347607 40.0 37.5 41.0 30.0 41.0 56-57 36.352561917345874 40.0 37.0 41.0 30.0 41.0 58-59 36.21888825342984 40.0 37.0 41.0 29.0 41.0 60-61 36.05072371899438 40.0 36.5 41.0 29.0 41.0 62-63 35.83543363506682 40.0 36.0 41.0 28.0 41.0 64-65 35.562951297228615 39.0 35.5 41.0 28.0 41.0 66-67 35.27684540595973 39.0 35.0 41.0 28.0 41.0 68-69 34.95587253380063 39.0 35.0 41.0 26.5 41.0 70-71 34.58444969089456 37.5 35.0 40.0 26.0 41.0 72-73 34.216815185655776 37.0 35.0 40.0 26.0 41.0 74-75 33.854516188710676 37.0 35.0 39.0 25.5 41.0 76-77 33.46916834061607 36.0 34.5 39.0 25.0 41.0 78-79 33.0858019978457 36.0 34.0 38.5 24.0 40.0 80-81 32.70944041726182 35.0 34.0 37.0 23.0 39.0 82-83 32.35559709516954 35.0 34.0 37.0 22.0 39.0 84-85 32.03474818448096 35.0 34.0 36.5 21.5 38.0 86-87 31.850378974632036 35.0 34.0 36.0 20.5 37.0 88-89 31.622719038509757 35.0 34.0 36.0 20.0 37.0 90-91 31.409356931089633 35.0 34.0 35.5 19.5 36.5 92-93 31.224719138054574 35.0 34.0 35.0 18.0 36.0 94-95 31.105074250103534 35.0 34.0 35.0 16.0 36.0 96-97 31.029805220843933 35.0 34.0 35.0 6.0 36.0 98-99 30.899978588473274 35.0 34.0 35.0 2.0 36.0 100 30.80196122071997 35.0 34.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2128041100442104 1101 2 0.2778662499255873 1101 3 0.2675284073290882 1101 4 0.1663716589767148 1101 5 0.12245603310407205 1101 6 0.10439314471278038 1101 7 0.13561732639242763 1101 8 0.0546452187011468 1101 9 0.2022801520901254 1101 10-11 0.2621871440268393 1101 12-13 0.28835529437370866 1101 14-15 0.3050609967996536 1101 16-17 0.2613544204988685 1101 18-19 0.2619605630238908 1101 20-21 0.3071966538010642 1101 22-23 0.3024334851095887 1101 24-25 0.3089435727726624 1101 26-27 0.30030315161372556 1101 28-29 0.28636037933554803 1101 30-31 0.23033967703049996 1101 32-33 0.32129311848362363 1101 34-35 0.29452865118121707 1101 36-37 0.26870373485365207 1101 38-39 0.3103167841974823 1101 40-41 0.4114699566062683 1101 42-43 0.4399000338076817 1101 44-45 0.4194840835197553 1101 46-47 0.4948147792397677 1101 48-49 0.4582116518776971 1101 50-51 0.5285478895486833 1101 52-53 0.6086862453661723 1101 54-55 0.643312839309651 1101 56-57 0.5811364829075316 1101 58-59 0.5907442984001392 1101 60-61 0.6183330782828165 1101 62-63 0.5782340995061546 1101 64-65 0.5982417345548683 1101 66-67 0.7321002899424727 1101 68-69 0.768260007595984 1101 70-71 0.6427767705550593 1101 72-73 0.5371600885486174 1101 74-75 0.6670759782957418 1101 76-77 0.6296842247644037 1101 78-79 0.523290010002583 1101 80-81 0.645986130150682 1101 82-83 0.5769420571346728 1101 84-85 0.5999758681512048 1101 86-87 0.6448501311951702 1101 88-89 0.627868723786019 1101 90-91 0.6097435192856757 1101 92-93 0.6029956408800139 1101 94-95 0.6252629990484344 1101 96-97 0.5127671259892104 1101 98-99 0.5936297410428608 1101 100 0.6314671401513152 1102 1 0.2641467785056406 1102 2 0.24984210317526845 1102 3 0.20034110919306158 1102 4 0.12775851571246477 1102 5 0.15917577609053524 1102 6 0.14291247567561527 1102 7 0.10767519839706807 1102 8 0.12790994575827597 1102 9 0.21211114520858843 1102 10-11 0.18508710205253465 1102 12-13 0.20673081911951385 1102 14-15 0.3082441822043904 1102 16-17 0.25442651605661837 1102 18-19 0.23486408591703878 1102 20-21 0.18989991691454833 1102 22-23 0.21870064538977374 1102 24-25 0.22924948664653044 1102 26-27 0.265578930296023 1102 28-29 0.2970300544287028 1102 30-31 0.31333032742850264 1102 32-33 0.30170752226356967 1102 34-35 0.3471552079963587 1102 36-37 0.4588746715882479 1102 38-39 0.4441676397924752 1102 40-41 0.4601756952923637 1102 42-43 0.34356552165576915 1102 44-45 0.31046105554813863 1102 46-47 0.4031606968232424 1102 48-49 0.3849327189577778 1102 50-51 0.27975418172735544 1102 52-53 0.3820877239552587 1102 54-55 0.3951953841639906 1102 56-57 0.39019655333644465 1102 58-59 0.4382454335289907 1102 60-61 0.5103283158343288 1102 62-63 0.4865944909875708 1102 64-65 0.5865982877729792 1102 66-67 0.5283710633364436 1102 68-69 0.4446099800151657 1102 70-71 0.41616109489473985 1102 72-73 0.49287961013803994 1102 74-75 0.4468792083336979 1102 76-77 0.49324200536905494 1102 78-79 0.5474590072348491 1102 80-81 0.4688240112363502 1102 82-83 0.460247327188128 1102 84-85 0.4698330107786006 1102 86-87 0.5074764896642989 1102 88-89 0.4557960562412724 1102 90-91 0.4554584681954985 1102 92-93 0.4543773888658613 1102 94-95 0.5104479995871856 1102 96-97 0.5028058677893448 1102 98-99 0.4320592266244425 1102 100 0.3406425729074627 1103 1 0.11295386001154384 1103 2 0.12119501332686156 1103 3 0.14090775801157207 1103 4 0.09618984343466508 1103 5 0.05041634314043364 1103 6 0.05390282764522425 1103 7 0.10559007272801324 1103 8 0.043626047801588186 1103 9 0.08387967670657304 1103 10-11 0.14059255576665208 1103 12-13 0.17330889677302252 1103 14-15 0.1963380093500362 1103 16-17 0.22188145949400706 1103 18-19 0.15591808961902842 1103 20-21 0.12619052898591576 1103 22-23 0.16202448310392725 1103 24-25 0.10673419037720322 1103 26-27 0.14896079456583067 1103 28-29 0.17381612324236073 1103 30-31 0.20461125336667862 1103 32-33 0.1700046880167605 1103 34-35 0.15458891413552323 1103 36-37 0.08543061953580633 1103 38-39 0.13586559086446215 1103 40-41 0.11971237525490608 1103 42-43 0.16818810338126866 1103 44-45 0.11789501236362554 1103 46-47 -0.09711630051557307 1103 48-49 -0.022008959691213192 1103 50-51 -0.04771959251554847 1103 52-53 -0.06562520552849094 1103 54-55 -0.0718692562358072 1103 56-57 0.03489469893326458 1103 58-59 -0.01823665514625361 1103 60-61 -0.021120282483934716 1103 62-63 -0.02938261524504071 1103 64-65 -0.019492695700435547 1103 66-67 0.06425588057334863 1103 68-69 0.04201803695278272 1103 70-71 0.12443576996808758 1103 72-73 0.04494107196204311 1103 74-75 -0.11690061964218046 1103 76-77 -0.006248871015429813 1103 78-79 -0.007372177301029126 1103 80-81 0.03590875587739362 1103 82-83 0.06986056370023874 1103 84-85 0.14751940868013946 1103 86-87 -0.004296273250041338 1103 88-89 0.07750492677397247 1103 90-91 0.028833684529168124 1103 92-93 0.09167857747272379 1103 94-95 0.002875067817271315 1103 96-97 0.008758929148893202 1103 98-99 0.055068605929811554 1103 100 -0.014346186766136526 1104 1 0.11080645313907667 1104 2 0.1517936507551667 1104 3 0.17849693080140838 1104 4 0.050401741291459246 1104 5 0.13103580506599144 1104 6 0.11623016581595635 1104 7 0.10198972730882616 1104 8 0.07070267048844414 1104 9 0.11286871423269673 1104 10-11 0.1822969548939284 1104 12-13 0.21963719401693282 1104 14-15 0.2906944750698557 1104 16-17 0.2546279814208745 1104 18-19 0.2068559561970531 1104 20-21 0.23719465185427424 1104 22-23 0.19542917377417268 1104 24-25 0.23247737656348733 1104 26-27 0.23251861215852188 1104 28-29 0.270791412779289 1104 30-31 0.31551459713136865 1104 32-33 0.38695773928323973 1104 34-35 0.34339402740932456 1104 36-37 0.3461170255332604 1104 38-39 0.3190939419845975 1104 40-41 0.3271974446635255 1104 42-43 0.4186232207435481 1104 44-45 0.4168555069549953 1104 46-47 0.30910229556941715 1104 48-49 0.3815922454549181 1104 50-51 0.38815958336930123 1104 52-53 0.5405357888508036 1104 54-55 0.43714832422508465 1104 56-57 0.5325844215662485 1104 58-59 0.6251734422512527 1104 60-61 0.5973820922729516 1104 62-63 0.677192576474063 1104 64-65 0.7241458024903125 1104 66-67 0.6944118541472548 1104 68-69 0.7894364679702051 1104 70-71 0.7573700944710708 1104 72-73 0.7516566857497722 1104 74-75 0.8003864833072498 1104 76-77 0.7069849059370128 1104 78-79 0.6965937431226479 1104 80-81 0.6852896569476599 1104 82-83 0.704532969521928 1104 84-85 0.7704689599026722 1104 86-87 0.7207897949702442 1104 88-89 0.7821743904189837 1104 90-91 0.6807278540520407 1104 92-93 0.7450896874375381 1104 94-95 0.7053761090421347 1104 96-97 0.7959698220804121 1104 98-99 0.9001773327315128 1104 100 0.888059520292412 1105 1 0.05203748982298606 1105 2 0.13188845035858776 1105 3 0.17965951619574838 1105 4 0.10012128114667718 1105 5 0.08185681309272042 1105 6 0.06224328564645987 1105 7 0.06479514365292971 1105 8 0.09906377820415457 1105 9 0.22183393174210408 1105 10-11 0.17125850536996978 1105 12-13 0.14277142101988005 1105 14-15 0.17919628821412914 1105 16-17 0.1823167996854167 1105 18-19 0.22234849310506632 1105 20-21 0.20072328866857703 1105 22-23 0.20077693077209346 1105 24-25 0.21984105120255748 1105 26-27 0.20422201706690402 1105 28-29 0.2664446200753332 1105 30-31 0.39339219970644024 1105 32-33 0.3247777558527929 1105 34-35 0.28244521647884824 1105 36-37 0.29364788973079214 1105 38-39 0.276567899256861 1105 40-41 0.41036779873597595 1105 42-43 0.40029484469607013 1105 44-45 0.30354960846391066 1105 46-47 0.2588714082831629 1105 48-49 0.4362296845074738 1105 50-51 0.5029085273995051 1105 52-53 0.3772441510450477 1105 54-55 0.2801008293521363 1105 56-57 0.338101948627596 1105 58-59 0.23759178285074256 1105 60-61 0.3672037657983367 1105 62-63 0.3725598909832968 1105 64-65 0.3348508236097061 1105 66-67 0.3759008055115274 1105 68-69 0.35684401119365816 1105 70-71 0.3767802453627027 1105 72-73 0.46274538721251446 1105 74-75 0.4483247956570011 1105 76-77 0.3969372345031985 1105 78-79 0.42814245354924196 1105 80-81 0.43916380979504055 1105 82-83 0.41789047811229807 1105 84-85 0.3958264956562445 1105 86-87 0.47676773844360554 1105 88-89 0.4583101521433015 1105 90-91 0.4587759057707892 1105 92-93 0.45577094074804947 1105 94-95 0.37747677589894124 1105 96-97 0.33927273261837954 1105 98-99 0.5138838885722841 1105 100 0.5271147838434587 1106 1 0.0228799022495636 1106 2 0.07569109317113032 1106 3 0.03768416035900657 1106 4 0.039983916058815794 1106 5 0.013505576424655885 1106 6 0.06463889200246342 1106 7 0.07986707555654249 1106 8 0.08716092768744232 1106 9 0.11966386917600147 1106 10-11 0.07050924125437064 1106 12-13 0.10189511331189038 1106 14-15 0.11395424277407074 1106 16-17 0.07711846131875433 1106 18-19 0.07204521953445919 1106 20-21 0.09020109745330984 1106 22-23 0.10282466450299665 1106 24-25 0.14240052722738028 1106 26-27 0.12060643543151883 1106 28-29 0.1247935630777377 1106 30-31 0.21473921857821665 1106 32-33 0.3334968710075543 1106 34-35 0.264504291112182 1106 36-37 0.2264020724849729 1106 38-39 0.16793352230565262 1106 40-41 0.27141944444107935 1106 42-43 0.2839263545652031 1106 44-45 0.22510688002117973 1106 46-47 0.19528080211829746 1106 48-49 0.32958123974021447 1106 50-51 0.4536960192761015 1106 52-53 0.4824724344911573 1106 54-55 0.4140445326027731 1106 56-57 0.392521077799195 1106 58-59 0.23401111590374057 1106 60-61 0.23161818021274883 1106 62-63 0.27440884035699753 1106 64-65 0.22587239680949978 1106 66-67 0.27832380842958315 1106 68-69 0.32626562516973223 1106 70-71 0.28746235604481996 1106 72-73 0.2855278585098304 1106 74-75 0.3181860288845755 1106 76-77 0.2583934573455622 1106 78-79 0.23476286462509677 1106 80-81 0.27532281179562546 1106 82-83 0.27635346726835053 1106 84-85 0.3804003198340311 1106 86-87 0.2767002027325063 1106 88-89 0.22905380952062515 1106 90-91 0.28191899228021455 1106 92-93 0.2543807245063441 1106 94-95 0.3496367718213129 1106 96-97 0.32126363441126315 1106 98-99 0.2100904204223788 1106 100 0.18763952357612013 1107 1 0.06788695165359115 1107 2 0.10131147306286437 1107 3 0.11045955599872315 1107 4 0.09166688043022475 1107 5 0.1597338218991311 1107 6 0.13819441090974038 1107 7 0.14850660525134884 1107 8 0.10999549951699095 1107 9 0.16455288956753122 1107 10-11 0.17075993164324643 1107 12-13 0.11749798287204527 1107 14-15 0.0956981456863133 1107 16-17 0.1599562344831682 1107 18-19 0.17449723184828514 1107 20-21 0.14825156536559092 1107 22-23 0.20578961797046702 1107 24-25 0.20395427408263345 1107 26-27 0.23744464837526635 1107 28-29 0.283393431790671 1107 30-31 0.3481055353616185 1107 32-33 0.3492400010722889 1107 34-35 0.3590014713505667 1107 36-37 0.3445272867157172 1107 38-39 0.40356551107834804 1107 40-41 0.2922244831481322 1107 42-43 0.37036279998405774 1107 44-45 0.5075697089664217 1107 46-47 0.5399784699943595 1107 48-49 0.5003267788902761 1107 50-51 0.49216296791893654 1107 52-53 0.4392030793499444 1107 54-55 0.3631190557978954 1107 56-57 0.46647110463867136 1107 58-59 0.3750747836633934 1107 60-61 0.41655468707136833 1107 62-63 0.36357975930178554 1107 64-65 0.34129102793165345 1107 66-67 0.34791039968702364 1107 68-69 0.4060491955311889 1107 70-71 0.3538112336374084 1107 72-73 0.32954742545653204 1107 74-75 0.3242002538058415 1107 76-77 0.40594265765858495 1107 78-79 0.3116559368071137 1107 80-81 0.42816512826859565 1107 82-83 0.4765807943800979 1107 84-85 0.4181518186408013 1107 86-87 0.366888515596699 1107 88-89 0.3757764025371877 1107 90-91 0.37382640579566484 1107 92-93 0.32563386925195204 1107 94-95 0.3052522041955399 1107 96-97 0.3982787776952499 1107 98-99 0.32780414994515183 1107 100 0.38910211322966504 1108 1 0.040504669874330546 1108 2 0.014482265295633567 1108 3 0.03782477716628563 1108 4 -0.05542816268659578 1108 5 0.008607821526901205 1108 6 0.02260876663900291 1108 7 -0.05720094484480853 1108 8 -0.01496262443610874 1108 9 -0.07759767808554585 1108 10-11 -0.022366968288508815 1108 12-13 -0.03708434233423219 1108 14-15 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-0.4522232502764112 2214 66-67 -0.38265442469114674 2214 68-69 -0.40548959787201255 2214 70-71 -0.46101233963644006 2214 72-73 -0.46705620391533387 2214 74-75 -0.44847028108286224 2214 76-77 -0.4540531182481118 2214 78-79 -0.44124313698316087 2214 80-81 -0.5086339684451104 2214 82-83 -0.42770550127037055 2214 84-85 -0.3684484044119287 2214 86-87 -0.3731193466257423 2214 88-89 -0.41655033236747485 2214 90-91 -0.3609134132379914 2214 92-93 -0.3175567764419185 2214 94-95 -0.2748622970652477 2214 96-97 -0.2851336393360917 2214 98-99 -0.31528250320785034 2214 100 -0.2804714078869388 2215 1 -0.16581487837347453 2215 2 -0.1668442031758275 2215 3 -0.17399773293723797 2215 4 -0.1575678958427673 2215 5 -0.08934102417979517 2215 6 -0.06111209921937899 2215 7 -0.07428836111009929 2215 8 -0.023756844665307142 2215 9 -0.14824208931291594 2215 10-11 -0.15176684649257055 2215 12-13 -0.2113189655972576 2215 14-15 -0.24326075569653938 2215 16-17 -0.2214403473297395 2215 18-19 -0.23777917885495725 2215 20-21 -0.22590825340940768 2215 22-23 -0.20783993988358418 2215 24-25 -0.27046910088888154 2215 26-27 -0.34121252638743016 2215 28-29 -0.27671669157152934 2215 30-31 -0.34118233398010744 2215 32-33 -0.32578685217751513 2215 34-35 -0.3692106217507174 2215 36-37 -0.32668095929760455 2215 38-39 -0.32352879202661455 2215 40-41 -0.31485629317994324 2215 42-43 -0.244475686168677 2215 44-45 -0.4220109296526786 2215 46-47 -0.3864832168654573 2215 48-49 -0.43468350546623213 2215 50-51 -0.3869808202796534 2215 52-53 -0.40568638416093705 2215 54-55 -0.47029752886933807 2215 56-57 -0.44026830992292076 2215 58-59 -0.44517447583790215 2215 60-61 -0.5314509427218468 2215 62-63 -0.458526849977801 2215 64-65 -0.39021935156924314 2215 66-67 -0.4500193649808537 2215 68-69 -0.4915169235545207 2215 70-71 -0.5414559280304232 2215 72-73 -0.5387666473990222 2215 74-75 -0.4951670423160266 2215 76-77 -0.5673881217992403 2215 78-79 -0.556907890050141 2215 80-81 -0.4650698871172452 2215 82-83 -0.5583678652108297 2215 84-85 -0.6698648716867694 2215 86-87 -0.7043641415605393 2215 88-89 -0.6652134558228759 2215 90-91 -0.7526780647383653 2215 92-93 -0.7130614133393784 2215 94-95 -0.7783506005606782 2215 96-97 -0.6221977642070904 2215 98-99 -0.7182876693906906 2215 100 -0.6510043733637403 2216 1 -0.22757905831960556 2216 2 -0.25631638833323933 2216 3 -0.23875717933101726 2216 4 -0.10255440568410279 2216 5 -0.20652909305898248 2216 6 -0.10836812144537333 2216 7 -0.2174986726956405 2216 8 -0.16796533965731442 2216 9 -0.23000765874181184 2216 10-11 -0.2787225971964489 2216 12-13 -0.23112386980292854 2216 14-15 -0.33653444154307977 2216 16-17 -0.3526682800396159 2216 18-19 -0.279589956609577 2216 20-21 -0.286669355722843 2216 22-23 -0.3566554535779858 2216 24-25 -0.31490951058098204 2216 26-27 -0.3547620126249953 2216 28-29 -0.38177973278640565 2216 30-31 -0.38732593439733876 2216 32-33 -0.465003713908942 2216 34-35 -0.4867975103812867 2216 36-37 -0.379806829962547 2216 38-39 -0.41397421197390116 2216 40-41 -0.39768725209732736 2216 42-43 -0.45393161772017976 2216 44-45 -0.4248643829112737 2216 46-47 -0.4486946813396955 2216 48-49 -0.5124516857857699 2216 50-51 -0.46218561977276096 2216 52-53 -0.43898502817377505 2216 54-55 -0.4652218581633747 2216 56-57 -0.528458924038496 2216 58-59 -0.5333444387062443 2216 60-61 -0.4847048749938665 2216 62-63 -0.5285224022005082 2216 64-65 -0.6356026041000931 2216 66-67 -0.6625794296705934 2216 68-69 -0.695356551459632 2216 70-71 -0.7413638210803555 2216 72-73 -0.7271485183256203 2216 74-75 -0.7140084285614421 2216 76-77 -0.7623135731841728 2216 78-79 -0.6917302141200139 2216 80-81 -0.8871086832903963 2216 82-83 -0.8031979508072631 2216 84-85 -0.8045856107197018 2216 86-87 -0.7621426307916863 2216 88-89 -0.7602861876056686 2216 90-91 -0.8341914024597905 2216 92-93 -0.8663433262608287 2216 94-95 -0.9803874820170293 2216 96-97 -0.9632971602118268 2216 98-99 -0.897501222147703 2216 100 -0.8640304638868699 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 1.0 4 5.0 5 29.0 6 132.0 7 320.0 8 440.0 9 693.0 10 1140.0 11 1963.0 12 3043.0 13 4309.0 14 5033.0 15 6878.0 16 9171.0 17 12998.0 18 14178.0 19 7131.0 20 3669.0 21 2944.0 22 2943.0 23 3182.0 24 3790.0 25 4481.0 26 5341.0 27 6471.0 28 6333.0 29 7997.0 30 9813.0 31 12238.0 32 15387.0 33 19944.0 34 26771.0 35 40675.0 36 66726.0 37 156584.0 38 366964.0 39 234296.0 40 828.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.578242050706066 22.07470960507793 14.741328064324977 21.605720279891028 2 15.026694025891091 22.911503552160173 40.54796707502031 21.513835346928424 3 17.108936776832728 27.90203663061454 33.5236893187472 21.46533727380553 4 11.53489245796938 19.417864187094956 40.81112050342819 28.236122851507467 5 10.794224097937901 39.06425202706891 37.01971567506043 13.121808199932762 6 26.32578390013561 41.08408683663848 18.507161881435458 14.082967381790452 7 22.390959734644582 36.273567009379846 24.490062319455767 16.8454109365198 8 23.04225420981167 35.55367909518669 22.68389834014752 18.72016835485412 9 24.13045050256707 16.366416142249534 23.86731064871205 35.635822706471345 10-11 21.72114867206275 27.799486686819797 30.63285148007179 19.84651316104567 12-13 23.608567056378547 26.26613319384531 30.633429325259552 19.491870424516584 14-15 20.789269799830024 27.80883602777869 29.60027046189821 21.801623710493075 16-17 18.047381454800547 31.188424252498248 30.47353281739067 20.290661475310536 18-19 17.972052270518244 28.65252689358545 34.453887655010824 18.92153318088548 20-21 19.67756870235818 27.610821085094855 34.01798568245015 18.693624530096812 22-23 18.996878421856305 27.77875861861715 34.299372867062466 18.924990092464075 24-25 19.20761841796964 27.996869014655246 34.03782068769924 18.757691879675875 26-27 18.762948659913484 27.80897873931002 34.422679947632474 19.005392653144025 28-29 18.60244955919768 27.883294216647275 34.621601238961155 18.89265498519389 30-31 19.453899430258833 28.31362814104334 33.833195265494126 18.3992771632037 32-33 18.919644941124865 27.937851077588938 34.4650940644905 18.6774099167957 34-35 19.059653915141904 27.69979865024745 34.2578263150553 18.98272111955535 36-37 19.402865890491906 27.7779343530139 33.827531751109255 18.991668005384934 38-39 19.218535748057683 27.70581926771901 34.05614407924189 19.019500904981417 40-41 19.11176967198751 27.453140569599828 33.919570486345854 19.515519272066815 42-43 19.303575909248533 27.426456146220303 33.48325757241143 19.78671037211973 44-45 19.504401367486334 27.7718367947459 33.062596860873356 19.66116497689441 46-47 19.880191498043462 28.177515527249547 32.26776819410049 19.674524780606497 48-49 19.904772380699885 27.982667426306477 31.813254586513384 20.299305606480253 50-51 19.582847580008274 28.08375014102516 31.688635252529053 20.644767026437517 52-53 19.677999197136767 28.16338924858958 31.430544339391158 20.72806721488249 54-55 19.663825884447906 28.51547387190202 31.06409224009992 20.756608003550156 56-57 19.7469284963251 27.96176430912495 30.851626361137107 21.439680833412847 58-59 19.344139947390957 27.7585322856642 31.396611759442155 21.50071600750269 60-61 19.540356294369367 28.233873109198278 30.841011686135726 21.384758910296632 62-63 19.13693767450954 28.262544125270416 30.921034388924635 21.679483811295412 64-65 18.966903226472912 28.191139196523068 31.004688936532666 21.837268640471354 66-67 18.985215716436258 28.115306706033827 30.68440818258398 22.215069394945942 68-69 19.552648717583914 28.353409015674103 30.36708142167103 21.726860845070952 70-71 19.692969975968378 27.982517676248325 30.24143374588086 22.083078601902432 72-73 19.518062219267183 27.872830556878124 30.679055300902387 21.930051922952313 74-75 19.73217748653093 28.161886167684187 30.096060748633374 22.009875597151517 76-77 19.787180493705193 28.039312726910747 30.022510297263644 22.15099648212042 78-79 19.969535529517383 27.865364695585377 30.308908228130427 21.856191546766812 80-81 19.970540271880328 27.849303677019204 29.999244020365435 22.180912030735033 82-83 19.57287720477891 27.709828463458564 30.266254463086682 22.451039868675846 84-85 19.594176440371246 27.741356269961788 29.866591162861894 22.797876126805072 86-87 19.84873798059992 27.895056632868194 29.523187029800695 22.733018356731193 88-89 19.60204462066239 27.60162765778617 29.562667923816 23.23365979773544 90-91 19.517667116966734 27.322273512918073 29.613826606770655 23.54623276334454 92-93 19.686439535909102 27.510894885543248 29.317372793612527 23.485292784935126 94-95 19.473233436886055 27.300589010889944 29.441669890950344 23.78450766127366 96-97 19.337518294924752 27.298633744175792 29.406312377065387 23.957535583834073 98-99 19.66573601653571 27.282370143316363 28.90293159486762 24.14896224528031 100 19.653245959278657 27.169349211670784 28.67238204173933 24.50502278731123 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 914.0 1 544.5 2 435.0 3 714.5 4 424.0 5 199.5 6 484.5 7 919.0 8 1422.0 9 1622.5 10 1107.0 11 572.0 12 528.0 13 618.5 14 708.0 15 779.5 16 842.5 17 945.0 18 1104.5 19 1299.5 20 1585.5 21 1998.5 22 2672.5 23 3749.0 24 5084.5 25 6720.5 26 9092.0 27 12378.5 28 16870.5 29 20980.5 30 24105.0 31 28126.5 32 32482.5 33 36383.5 34 40240.0 35 43135.5 36 44348.0 37 45023.5 38 45392.0 39 44903.0 40 44098.5 41 42322.0 42 40053.0 43 38013.0 44 35989.5 45 34281.5 46 32436.0 47 32125.0 48 33522.5 49 32091.5 50 29606.0 51 27602.5 52 24194.5 53 20696.5 54 18061.5 55 16655.5 56 15215.5 57 13619.0 58 11887.5 59 9781.5 60 7750.5 61 6039.0 62 4641.5 63 3570.0 64 2663.0 65 1889.0 66 1378.0 67 994.5 68 716.5 69 536.0 70 400.5 71 287.5 72 203.5 73 170.0 74 132.5 75 81.5 76 56.5 77 40.5 78 25.0 79 9.5 80 5.0 81 3.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.75640819760961E-4 2 1.878204098804805E-4 3 0.0 4 0.013804800126215317 5 8.451918444621621E-4 6 0.004038138812430331 7 0.0010330122543426427 8 0.1295021726125913 9 0.0013147428691633633 10-11 0.0 12-13 4.6955102470120124E-5 14-15 1.878204098804805E-4 16-17 0.0 18-19 1.878204098804805E-4 20-21 0.0 22-23 9.391020494024025E-5 24-25 6.104163321115616E-4 26-27 0.006104163321115616 28-29 0.007888457214980181 30-31 0.014368261355856757 32-33 0.016762971581832882 34-35 0.02643572269067763 36-37 0.037188441156335135 38-39 0.04324564937498063 40-41 0.053857502533227776 42-43 0.07080829452494114 44-45 0.10738631934916472 46-47 0.11579128269131622 48-49 0.11128359285418468 50-51 0.11334961736286997 52-53 0.10837237650103723 54-55 0.11565041738390586 56-57 0.10109433561816862 58-59 0.090294662050041 60-61 0.09198504573896531 62-63 0.09132767430438364 64-65 0.0 66-67 0.0 68-69 1.4086530741036035E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 9.391020494024025E-5 78-79 0.0 80-81 3.286857172908408E-4 82-83 9.391020494024025E-5 84-85 0.0 86-87 5.634612296414414E-4 88-89 3.75640819760961E-4 90-91 1.4086530741036035E-4 92-93 0.0011738775617530029 94-95 0.0010330122543426427 96-97 2.347755123506006E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1064847.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.67665050139559 #Duplication Level Percentage of deduplicated Percentage of total 1 85.86489176489354 64.97967404432008 2 10.635513547983505 16.097200833472105 3 2.1509201355708543 4.883232940680298 4 0.6318541470756907 1.9126642182441775 5 0.25315268927907614 0.9578873795030517 6 0.10693280731773128 0.4855390011910216 7 0.06708263273335115 0.3553612266452706 8 0.04323459345402317 0.2617479374712029 9 0.0275083356089546 0.18735648297785568 >10 0.1704039111408713 2.5018063988808157 >50 0.02413913739331103 1.290585700915906 >100 0.02175562603449534 3.240663711886101 >500 0.001491812294063678 0.7736154378960424 >1k 8.702238382038123E-4 1.0848303069900602 >5k 2.4863538234394636E-4 0.9878343789259937 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5394 0.5065516454476559 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5106 0.47950550642486667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2689 0.25252454108430605 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 2529 0.23749890829386755 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1753 0.16462458926024115 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1371 0.12875089097306938 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1085 0.10189257236016065 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1065 0.10001436826135586 RNA PCR Primer, Index 23 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.4086530741036035E-4 0.0 16-17 0.0 0.0 0.0 2.817306148207207E-4 0.0 18-19 0.0 0.0 0.0 3.7564081976096094E-4 0.0 20-21 0.0 0.0 0.0 7.98236741992042E-4 0.0 22-23 0.0 0.0 0.0 0.0021129796111554054 0.0 24-25 0.0 0.0 0.0 0.006245028628525976 0.0 26-27 0.0 0.0 0.0 0.015119542995378678 0.0 28-29 0.0 0.0 0.0 0.03925446566502042 0.0 30-31 0.0 0.0 0.0 0.09001293143522027 0.0 32-33 0.0 0.0 0.0 0.17673900569753215 0.0 34-35 0.0 0.0 0.0 0.32286328458454594 0.0 36-37 0.0 0.0 0.0 0.5290431395308435 0.0 38-39 0.0 0.0 0.0 0.7939638276672611 0.0 40-41 0.0 0.0 0.0 1.0773378710744361 0.0 42-43 0.0 0.0 0.0 1.330989334618025 0.0 44-45 0.0 0.0 0.0 1.6280273128440048 0.0 46-47 0.0 0.0 0.0 1.9765280833772363 0.0 48-49 0.0 0.0 0.0 2.3401483969058465 0.0 50-51 0.0 0.0 0.0 2.6942837797354926 0.0 52-53 0.0 0.0 0.0 3.0355065093858555 0.0 54-55 0.0 0.0 0.0 3.434765745689287 0.0 56-57 0.0 0.0 0.0 3.9017342397546315 0.0 58-59 0.0 0.0 0.0 4.385653525811689 0.0 60-61 0.0 0.0 0.0 4.852575064774564 0.0 62-63 0.0 0.0 0.0 5.332033616096961 0.0 64-65 4.695510247012012E-5 0.0 0.0 5.849995351444855 0.0 66-67 9.391020494024023E-5 0.0 0.0 6.447452075274664 0.0 68-69 9.391020494024023E-5 0.0 0.0 7.110552032357701 0.0 70-71 9.391020494024023E-5 0.0 0.0 7.792950536555956 0.0 72-73 9.391020494024023E-5 0.0 0.0 8.49389630622991 0.0 74-75 9.391020494024023E-5 0.0 0.0 9.213248476072149 0.0 76-77 9.391020494024023E-5 0.0 0.0 9.987350295394549 0.0 78-79 9.391020494024023E-5 0.0 0.0 10.834467299057987 0.0 80-81 9.391020494024023E-5 0.0 0.0 11.709240858076324 0.0 82-83 9.391020494024023E-5 0.0 0.0 12.582324033405738 0.0 84-85 9.391020494024023E-5 0.0 0.0 13.464939094536586 0.0 86-87 9.391020494024023E-5 0.0 0.0 14.398265666335163 0.0 88 9.391020494024023E-5 0.0 0.0 15.132878244480192 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 75 7.3879146E-6 37.640785 3 GGTATCA 6090 0.0 32.254993 1 GTATCAA 8995 0.0 29.517885 1 TATAACG 100 5.283982E-5 28.230587 2 CGGGTCA 70 0.008801244 26.878689 6 ATCAACG 10365 0.0 25.511482 3 TATCAAC 10410 0.0 25.491598 2 TCAACGC 10395 0.0 25.437857 4 CAACGCA 10435 0.0 25.334389 5 AACGCAG 10790 0.0 24.630495 6 GAGTACT 7090 0.0 23.356516 12-13 CGCAGAG 11650 0.0 22.690083 8 ACGCAGA 11860 0.0 22.32903 7 GCAGAGT 12065 0.0 21.49349 9 AGTACTT 7340 0.0 20.896921 12-13 GTACTTT 8025 0.0 20.664495 14-15 AGAGTAC 9660 0.0 20.18209 10-11 GTATAGG 240 1.5818841E-7 19.580631 1 GTACATG 4360 0.0 19.508778 1 CATGGGG 1620 0.0 19.459356 4 >>END_MODULE