FastQCFastQC Report
Wed 25 May 2016
SRR1032976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430145
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.035.5815051
GTATCAA31450.035.1201671
TCAACGC31000.033.507344
ATCAACG32400.032.204563
CAACGCA33000.031.4752965
AACGCAG34850.029.6695796
TATCAAC38100.027.633282
GTACATG70200.027.4508691
TACATGG70350.026.256562
CGACCGT751.1639731E-625.066320-21
ACATGGG70900.024.7933243
ACGCAGA41750.024.5409567
CGCAGAG43600.023.4996538
CATGGGG46350.018.7599374
GAGTACA51350.018.397761
GCAGAGT53900.018.2238469
AGTACAT50750.017.5039122
GTCTAGG6200.017.4359461
TCCGGTC3008.679308E-817.2330828
TACACGG2203.196184E-517.0913622
Produced by FastQC (version 0.11.5)