##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032976_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2430145
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70040635435334	33.0	31.0	34.0	30.0	34.0
2	31.729572103722205	34.0	31.0	34.0	30.0	34.0
3	31.791470467811592	34.0	31.0	34.0	30.0	34.0
4	35.11757158523463	37.0	35.0	37.0	33.0	37.0
5	35.16585594686737	37.0	35.0	37.0	33.0	37.0
6	35.24024451215874	37.0	36.0	37.0	33.0	37.0
7	35.146390853220694	37.0	36.0	37.0	33.0	37.0
8	35.15019062648525	37.0	36.0	37.0	33.0	37.0
9	36.78589096535392	39.0	38.0	39.0	34.0	39.0
10-11	36.782991549886944	39.0	38.0	39.0	34.0	39.0
12-13	36.74917278598602	39.0	38.0	39.0	34.0	39.0
14-15	38.23853062265832	41.0	39.0	41.0	34.0	41.0
16-17	38.283391731769086	41.0	39.0	41.0	34.0	41.0
18-19	38.30720553711815	41.0	39.0	41.0	34.0	41.0
20-21	38.341799357651496	41.0	39.0	41.0	34.0	41.0
22-23	38.35062537420606	41.0	39.0	41.0	34.0	41.0
24-25	38.39835215594131	41.0	39.0	41.0	34.0	41.0
26-27	38.38509965454736	41.0	39.0	41.0	34.0	41.0
28-29	38.37006516072086	41.0	39.0	41.0	34.0	41.0
30-31	38.33141582086665	41.0	39.0	41.0	34.0	41.0
32-33	38.276093813332125	41.0	39.0	41.0	33.5	41.0
34-35	38.21205380748886	41.0	39.0	41.0	33.0	41.0
36-37	38.1308195601497	41.0	39.0	41.0	33.0	41.0
38-39	38.043688751082755	41.0	38.0	41.0	33.0	41.0
40-41	37.96358591771273	41.0	38.0	41.0	33.0	41.0
42-43	37.86265696079863	40.5	38.0	41.0	33.0	41.0
44-45	37.75491997391102	40.0	38.0	41.0	32.5	41.0
46-47	37.647884796997715	40.0	38.0	41.0	32.0	41.0
48-49	37.52635069100815	40.0	38.0	41.0	32.0	41.0
50-51	37.406416078052956	40.0	38.0	41.0	32.0	41.0
52-53	37.25929810772608	40.0	37.0	41.0	31.0	41.0
54-55	37.110023887463505	40.0	37.0	41.0	31.0	41.0
56-57	36.9451621199558	40.0	36.5	41.0	31.0	41.0
58-59	36.75536603782902	40.0	36.0	41.0	31.0	41.0
60-61	36.53141540936858	39.5	35.5	41.0	31.0	41.0
62-63	36.29502025599295	39.0	35.0	41.0	30.0	41.0
64-65	36.02625625219894	39.0	35.0	41.0	30.0	41.0
66-67	35.74272996055791	38.0	35.0	40.0	30.0	41.0
68-69	35.44099919963624	37.5	35.0	40.0	30.0	41.0
70-71	35.106855146503605	37.0	35.0	40.0	29.0	41.0
72-73	34.81281939966545	37.0	35.0	39.0	29.0	41.0
74-75	34.53053192299225	36.0	34.0	39.0	29.0	41.0
76-77	34.198769826491834	36.0	34.0	39.0	29.0	40.5
78-79	33.82228056350547	35.0	34.0	37.5	29.0	39.5
80-81	33.4878017566853	35.0	34.0	37.0	28.0	39.0
82-83	33.154082986817656	35.0	34.0	37.0	28.0	39.0
84-85	32.96671515485701	35.0	34.0	36.0	28.0	38.0
86-87	32.72135633881929	35.0	34.0	36.0	28.0	37.0
88-89	32.47245370132235	35.0	34.0	36.0	27.0	37.0
90-91	32.26447022708521	35.0	34.0	35.0	27.0	36.5
92-93	32.06703036238578	35.0	34.0	35.0	27.0	36.0
94-95	31.880732219682365	35.0	34.0	35.0	26.5	36.0
96-97	31.70512952930792	35.0	33.5	35.0	26.0	36.0
98-99	31.528654051507218	35.0	33.0	35.0	25.0	36.0
100	31.408848854697972	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22744949231045553
1101	2	0.33679323540870953
1101	3	0.2834507541288147
1101	4	0.22844346252401238
1101	5	0.19606562201169453
1101	6	0.13658333582681337
1101	7	0.17210127798641395
1101	8	0.08625229555842395
1101	9	0.18503765286671836
1101	10-11	0.1923418521479192
1101	12-13	-0.033186110756233234
1101	14-15	-0.004867579528493593
1101	16-17	0.027425369164916447
1101	18-19	0.020475845921261282
1101	20-21	-0.0010350722933978318
1101	22-23	-0.018452246578128495
1101	24-25	-0.05193576809602263
1101	26-27	0.056454249174180404
1101	28-29	0.07186258689995384
1101	30-31	0.07523680188062087
1101	32-33	0.06617563486786793
1101	34-35	0.025141959453144125
1101	36-37	0.02362650891475937
1101	38-39	0.011122274987002356
1101	40-41	-0.002810264419686348
1101	42-43	0.017265051851822477
1101	44-45	0.05181392639475746
1101	46-47	0.01605588513186973
1101	48-49	-0.012112657732984644
1101	50-51	0.14137805984209706
1101	52-53	0.021088183072109246
1101	54-55	0.021990330420635473
1101	56-57	-0.005622389252032178
1101	58-59	0.017701061187274547
1101	60-61	0.08620869989666602
1101	62-63	0.013412691235068053
1101	64-65	0.0013042613433498218
1101	66-67	0.001810225803701826
1101	68-69	-0.0018416195848942607
1101	70-71	0.07690817446933096
1101	72-73	0.005418143776950046
1101	74-75	0.0012555536120402166
1101	76-77	0.014147016432133341
1101	78-79	0.042530615815017825
1101	80-81	0.08321068011276367
1101	82-83	0.04196410213604196
1101	84-85	-0.03259466044497117
1101	86-87	-0.008569967337727746
1101	88-89	-0.008447257310749023
1101	90-91	-0.01238320863587461
1101	92-93	0.06249928805574001
1101	94-95	0.025696921283838492
1101	96-97	-0.03468903762498954
1101	98-99	-0.029369340283853518
1101	100	0.09279650106135406
1102	1	0.31735233179283995
1102	2	0.3391677483161395
1102	3	0.36617534260993523
1102	4	0.3269322622130204
1102	5	0.25162980890091546
1102	6	0.2547068631349845
1102	7	0.228259753423103
1102	8	0.28256721604026325
1102	9	0.2995631875118008
1102	10-11	0.3580680336053419
1102	12-13	0.3448249865919095
1102	14-15	0.40623685291132006
1102	16-17	0.41338493433813994
1102	18-19	0.3487428693874577
1102	20-21	0.2880873908609516
1102	22-23	0.3545545167085393
1102	24-25	0.32882561175141944
1102	26-27	0.3595336260315136
1102	28-29	0.366737810359524
1102	30-31	0.36927582362129385
1102	32-33	0.34991629640158095
1102	34-35	0.3083088495440123
1102	36-37	0.2787933525945192
1102	38-39	0.35763782445251024
1102	40-41	0.40675524367674143
1102	42-43	0.41181835958169444
1102	44-45	0.43139512465215546
1102	46-47	0.44401938751388315
1102	48-49	0.4406061227567264
1102	50-51	0.3952280355658786
1102	52-53	0.3926556832614807
1102	54-55	0.4111686283989968
1102	56-57	0.4439203906090583
1102	58-59	0.3914350732924561
1102	60-61	0.5002679727270802
1102	62-63	0.40279092486046153
1102	64-65	0.4330845111728223
1102	66-67	0.48282747045371366
1102	68-69	0.5369153796956425
1102	70-71	0.4860062227408406
1102	72-73	0.4028047929054992
1102	74-75	0.4275567460284293
1102	76-77	0.42733575087203235
1102	78-79	0.3888589172223149
1102	80-81	0.37737214416051756
1102	82-83	0.33923015785521216
1102	84-85	0.2772569174052748
1102	86-87	0.3443043280883913
1102	88-89	0.27998738759853836
1102	90-91	0.2527730595376809
1102	92-93	0.30567225686946387
1102	94-95	0.31805670055186397
1102	96-97	0.37404941890954646
1102	98-99	0.37782740912758683
1102	100	0.34803428204074294
1103	1	0.07265107450717068
1103	2	0.1350654282860262
1103	3	0.09545270809136497
1103	4	0.11606251222190878
1103	5	-0.025593803697574913
1103	6	0.0196681810391226
1103	7	0.015298602465612987
1103	8	0.1513877155134935
1103	9	0.15347975699443595
1103	10-11	0.06931856348632692
1103	12-13	0.03971872961210465
1103	14-15	0.1557047776130105
1103	16-17	0.19012494796793789
1103	18-19	0.1997152575387915
1103	20-21	0.10547716912194005
1103	22-23	0.1355980192995645
1103	24-25	0.12961481087588567
1103	26-27	0.11465225623060604
1103	28-29	0.13757239944997934
1103	30-31	0.1453668589888224
1103	32-33	0.16144413327893403
1103	34-35	0.12212190314257754
1103	36-37	0.1096495193613265
1103	38-39	0.10888197560980473
1103	40-41	0.14776817998274083
1103	42-43	0.11888160934705638
1103	44-45	0.1358974807585156
1103	46-47	0.11363154911214934
1103	48-49	0.04874949231885495
1103	50-51	0.13161411332522732
1103	52-53	0.06569253521356444
1103	54-55	0.04177738451831203
1103	56-57	0.043953726202381915
1103	58-59	0.023478683986823512
1103	60-61	0.07299034876493948
1103	62-63	0.08508825596199188
1103	64-65	0.04640806355766358
1103	66-67	0.012544423322538023
1103	68-69	0.042429993215478135
1103	70-71	0.019007027112053265
1103	72-73	0.030437259122308546
1103	74-75	0.06107573885286399
1103	76-77	0.051182719540790345
1103	78-79	0.013133865828450553
1103	80-81	0.07483999147920173
1103	82-83	0.10243867020017916
1103	84-85	0.09715062683908826
1103	86-87	0.0960157328468938
1103	88-89	0.16071465882765779
1103	90-91	0.11873737397311146
1103	92-93	0.13402610460926923
1103	94-95	0.07772735886176463
1103	96-97	0.1137928351522568
1103	98-99	0.14527747716462258
1103	100	0.18449704420364554
1104	1	0.08276305694042918
1104	2	0.114441487210037
1104	3	0.1707135000459381
1104	4	0.03856368224460027
1104	5	0.08082302100639538
1104	6	0.08127768765488241
1104	7	0.16122842929051728
1104	8	0.1464505091778605
1104	9	0.19934682748495902
1104	10-11	0.1670341020503585
1104	12-13	0.24274994193345378
1104	14-15	0.3031812406321137
1104	16-17	0.3188422499337875
1104	18-19	0.27255689808215067
1104	20-21	0.24466368898413293
1104	22-23	0.2569983487598364
1104	24-25	0.3439742504025105
1104	26-27	0.2471064584093483
1104	28-29	0.22075830726804213
1104	30-31	0.25626353468152274
1104	32-33	0.292154773057149
1104	34-35	0.3236472067130265
1104	36-37	0.33097481972727394
1104	38-39	0.2761840211508968
1104	40-41	0.2643856563425828
1104	42-43	0.3104023485916798
1104	44-45	0.1905373515917006
1104	46-47	0.23525271275267556
1104	48-49	0.17397199376571137
1104	50-51	0.25086616452453114
1104	52-53	0.3301131749590738
1104	54-55	0.2672439234632762
1104	56-57	0.3395409685383868
1104	58-59	0.2463918513160479
1104	60-61	0.2695015629888573
1104	62-63	0.23294221330630194
1104	64-65	0.22791865994920357
1104	66-67	0.19991574494443398
1104	68-69	0.18128134006069985
1104	70-71	0.24489669525175373
1104	72-73	0.22445646553025256
1104	74-75	0.2987024605520503
1104	76-77	0.3237036744605035
1104	78-79	0.2677858752013904
1104	80-81	0.21204370142631745
1104	82-83	0.21480304382929205
1104	84-85	0.24324583812224176
1104	86-87	0.24349005673148838
1104	88-89	0.17592788985276542
1104	90-91	0.11612343549008841
1104	92-93	0.14403134003612905
1104	94-95	0.1201943379651027
1104	96-97	0.03380241679710849
1104	98-99	0.035959860234282104
1104	100	0.04310402806676805
1105	1	-0.04335480066946218
1105	2	-0.004517956366772324
1105	3	-0.05061165095361986
1105	4	-0.03191088940798892
1105	5	-0.05051918001335309
1105	6	0.0761020908175567
1105	7	0.05567936924931871
1105	8	0.06834705863762736
1105	9	0.08871579625484571
1105	10-11	0.05490042750712831
1105	12-13	0.12337824738415293
1105	14-15	0.12580189021201704
1105	16-17	0.07314933190835404
1105	18-19	0.07609967005721074
1105	20-21	0.0768287160336456
1105	22-23	0.12542909345679476
1105	24-25	0.0834848541517701
1105	26-27	0.0829508445244258
1105	28-29	0.09592981664482636
1105	30-31	0.10394983300297156
1105	32-33	0.10322542585593908
1105	34-35	0.09071360333174283
1105	36-37	0.12230081432453233
1105	38-39	0.16216526427551514
1105	40-41	0.10515328513766775
1105	42-43	0.08864057333336461
1105	44-45	0.06227762598575737
1105	46-47	0.050282554115206324
1105	48-49	0.03712549897865358
1105	50-51	0.05897395230113034
1105	52-53	0.06260805918756773
1105	54-55	0.08324873656286513
1105	56-57	0.07500621106386518
1105	58-59	0.10372445175876521
1105	60-61	0.029899831349446515
1105	62-63	0.11272172409859138
1105	64-65	0.04927689415981007
1105	66-67	-0.034814753787792085
1105	68-69	-0.07815855456934884
1105	70-71	-0.07729325217365357
1105	72-73	-0.08674902592826328
1105	74-75	-0.01028498128341937
1105	76-77	-0.07692982149301031
1105	78-79	-0.01301222946741376
1105	80-81	-0.03256208745879974
1105	82-83	0.008353815882344406
1105	84-85	-0.019930904299819474
1105	86-87	-0.0012341742389594401
1105	88-89	0.017505154902544007
1105	90-91	-0.07102251231208356
1105	92-93	-0.04111708602567177
1105	94-95	-0.0492648627248542
1105	96-97	-0.06974908121729584
1105	98-99	-0.07256487591179805
1105	100	-0.04988614228759758
1106	1	0.051401497469786506
1106	2	0.08626341298904094
1106	3	0.08525604150212374
1106	4	0.11391138161987868
1106	5	0.1053051641401126
1106	6	0.13865062990142007
1106	7	0.0743451951613352
1106	8	0.0716210735279077
1106	9	0.130800867783897
1106	10-11	0.13395044782252086
1106	12-13	0.18425682044565406
1106	14-15	0.18603551677563246
1106	16-17	0.11918154285444871
1106	18-19	0.21280656251189356
1106	20-21	0.23289123254694744
1106	22-23	0.17699846197278646
1106	24-25	0.2137554184189412
1106	26-27	0.1935314961450345
1106	28-29	0.18942933583048926
1106	30-31	0.15949354730869203
1106	32-33	0.1345107722781833
1106	34-35	0.14344670500668855
1106	36-37	0.16108707429791025
1106	38-39	0.2034494967911229
1106	40-41	0.17352558149466546
1106	42-43	0.14814778974598397
1106	44-45	0.13601863359942
1106	46-47	0.10038141818696289
1106	48-49	0.10795414212766019
1106	50-51	0.06579396807616433
1106	52-53	0.06677910705189305
1106	54-55	0.09680497248503173
1106	56-57	0.11943927028911361
1106	58-59	0.058535638366258524
1106	60-61	0.07745843640254435
1106	62-63	0.18537630351799805
1106	64-65	0.19267523284541
1106	66-67	0.16767104584597803
1106	68-69	0.14833566411531507
1106	70-71	0.1408039527757552
1106	72-73	0.11299312761781977
1106	74-75	0.10124280190323987
1106	76-77	0.07277491551736404
1106	78-79	0.20169482824655205
1106	80-81	0.1558215609515301
1106	82-83	0.11250649555847758
1106	84-85	0.06998981088887035
1106	86-87	0.12658412419209242
1106	88-89	0.09258071282945224
1106	90-91	0.13628705493324844
1106	92-93	0.08654337657748812
1106	94-95	0.15110575803051063
1106	96-97	0.15054010231401804
1106	98-99	0.13672823023432912
1106	100	0.0876963289729531
1107	1	0.15145434247848755
1107	2	0.19380111121261834
1107	3	0.18023857397193765
1107	4	0.1626192048876831
1107	5	0.06497148799152797
1107	6	0.12632235667764036
1107	7	0.10725010973229132
1107	8	0.10682960849086243
1107	9	0.030794010488378376
1107	10-11	0.0011784221410167106
1107	12-13	0.014610486762222763
1107	14-15	0.12634803411853568
1107	16-17	0.11667094060634042
1107	18-19	0.12941025919595717
1107	20-21	0.19594222237117975
1107	22-23	0.11543796439315912
1107	24-25	0.0926629346017549
1107	26-27	0.08373256200501089
1107	28-29	0.0764870783449112
1107	30-31	0.09232990378315264
1107	32-33	0.04390253313732018
1107	34-35	0.13585460903902202
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2216	44-45	-0.36717895506608755
2216	46-47	-0.3109790885604866
2216	48-49	-0.3489285042201473
2216	50-51	-0.3286531998298301
2216	52-53	-0.42276356561250594
2216	54-55	-0.3686853117953959
2216	56-57	-0.4286251747673546
2216	58-59	-0.36076195335930805
2216	60-61	-0.3492218169711805
2216	62-63	-0.38903206271108104
2216	64-65	-0.3888313004022379
2216	66-67	-0.3332826247069818
2216	68-69	-0.44705177124072293
2216	70-71	-0.4267445646207335
2216	72-73	-0.4387366070741834
2216	74-75	-0.34952686669126365
2216	76-77	-0.34251934073282797
2216	78-79	-0.3268506902821713
2216	80-81	-0.33224738240658525
2216	82-83	-0.3613262104606747
2216	84-85	-0.39968967746756334
2216	86-87	-0.3522920348090963
2216	88-89	-0.2801436361408278
2216	90-91	-0.3137177799760167
2216	92-93	-0.37137938727696707
2216	94-95	-0.3970988075926698
2216	96-97	-0.4457159642730879
2216	98-99	-0.49167419946057933
2216	100	-0.48227808884889
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33391.0
3	4897.0
4	3234.0
5	2787.0
6	2186.0
7	2430.0
8	2893.0
9	2984.0
10	3268.0
11	3535.0
12	3897.0
13	4228.0
14	4707.0
15	4893.0
16	4748.0
17	4794.0
18	4841.0
19	4694.0
20	4693.0
21	4881.0
22	5809.0
23	8279.0
24	13075.0
25	18413.0
26	19294.0
27	16073.0
28	15770.0
29	20380.0
30	24432.0
31	28965.0
32	37537.0
33	50369.0
34	72513.0
35	112750.0
36	209971.0
37	458743.0
38	805646.0
39	403204.0
40	941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3950671681803	19.91688292863314	13.160985380389082	23.527064522797485
2	15.451489839048953	20.119663173985593	40.79868517935886	23.63016180760659
3	18.105527932047544	25.812280760997673	29.92114602645652	26.161045280498264
4	12.08228232556703	16.789753832531993	39.53369230610837	31.594271535792608
5	12.512179392673502	38.44771735737447	35.65158412408444	13.38851912586759
6	30.354081464145654	38.71570982459623	18.033812368657195	12.896396342600925
7	27.16765171955973	32.86419777490059	22.591973960340912	17.376176545198774
8	24.903718809506554	37.08501900915206	20.416512924246717	17.594749257094662
9	24.784839099303667	17.46704174403564	21.349018386944934	36.39910076971575
10-11	23.571694924013915	27.494037511327342	28.94523405509148	19.989033509567264
12-13	24.14181195007183	24.884750971707494	29.01291979235923	21.960517285861446
14-15	21.499868430426464	26.973862746785603	26.006507237561937	25.519761585225993
16-17	21.146959308862797	30.381462553484212	26.561298400368806	21.910279737284185
18-19	21.068421611800954	29.241719080848654	28.545626898873095	21.144232408477304
20-21	22.25170414407566	28.625131953162107	28.002669226906317	21.120494675855912
22-23	21.93164604553375	28.402806074598463	28.06525517377983	21.600292706087963
24-25	21.702216240156204	28.501243323966968	28.098133335968846	21.698407099907985
26-27	21.48523751990005	28.395176878799333	28.22885241473143	21.890733186569186
28-29	21.534000679850944	28.378382551837607	28.310006265252635	21.777610503058813
30-31	21.76137307737685	28.887080165980638	27.865895252980877	21.485651503661636
32-33	21.372993838726334	28.5482709972105	28.392139002155336	21.68659616190783
34-35	21.55863803857064	28.1790242226174	28.490765831011384	21.77157190780058
36-37	21.902631864479083	28.668939239056783	27.846856044954027	21.581572851510103
38-39	21.676231967905885	28.779577369818394	28.085934323183302	21.458256339092415
40-41	21.46722076997959	28.439447247355076	28.364831075905894	21.728500906759436
42-43	21.34198294538975	28.45301910747238	28.334336085445187	21.870661861692685
44-45	21.51102036630984	28.549979110896327	28.310325806186594	21.62867471660724
46-47	21.52703849687611	28.804891478253854	28.08578536650052	21.582284658369517
48-49	21.440298499779153	28.59543869439294	28.225629261874047	21.738633543953867
50-51	21.47983976086859	28.421174233079817	28.224067087266246	21.87491891878535
52-53	21.499384708144092	28.45665116493611	28.252562892382404	21.791401234537393
54-55	21.586887784466775	28.90016648700073	28.04557440074461	21.467371327787884
56-57	21.408713754891796	28.58250392012923	28.130970925665288	21.877811399313682
58-59	21.28767571222833	28.17245692159877	28.51526384478309	22.02460352138981
60-61	21.649466704746192	28.657513441255983	28.0566078101877	21.636412043810125
62-63	21.42198080290911	28.598167597213198	28.10709023950548	21.872761360372206
64-65	21.40525220894268	28.342498696079836	28.305723308656482	21.946525786320997
66-67	21.29310128268643	28.401948223895022	28.178002135682995	22.12694835773555
68-69	21.73122804893442	28.58186515876217	28.06808384815448	21.61882294414893
70-71	21.65519848553296	28.3194958804734	28.201718641707224	21.823586992286415
72-73	21.392845516603217	28.483807356516948	28.328967127131033	21.7943799997488
74-75	21.48788187081257	28.459273050203958	28.24373348784316	21.80911159114031
76-77	21.522431145812742	28.464988139948606	28.066255682941293	21.94632503129736
78-79	21.64217642364208	28.515196140202626	28.124225285527327	21.718402150627966
80-81	21.495690998888517	28.3537503155514	28.289569322267656	21.860989363292425
82-83	21.35785333258384	28.41224336249567	28.400527364063016	21.829375940857474
84-85	21.437909142703127	28.343390679458203	28.20592964408175	22.012770533756918
86-87	21.643609459755517	28.39923930572234	28.151638918950972	21.80551231557117
88-89	21.368626886574997	28.360852759385853	28.203340051816532	22.067180302222617
90-91	21.35340249996397	28.26787622481145	28.329870883923185	22.048850391301393
92-93	21.585358145192657	28.381317930824185	28.155843546184368	21.87748037779879
94-95	21.358007126816524	28.20305765012713	28.301619130192364	22.13731609286398
96-97	21.452503522310646	28.12326470995782	28.335148964451996	22.089082803279542
98-99	21.398452436788208	28.19165867464318	28.296539827866436	22.11334906070218
100	21.561758795625245	28.10922881802609	28.194590855185137	22.134421531163525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2257.0
1	1441.0
2	537.0
3	406.0
4	325.5
5	268.0
6	241.0
7	266.0
8	287.0
9	279.0
10	278.5
11	263.5
12	273.5
13	307.0
14	343.5
15	418.5
16	508.0
17	702.5
18	1007.0
19	1407.0
20	2033.0
21	2909.0
22	4185.0
23	6294.0
24	9120.5
25	12698.0
26	17403.0
27	23199.0
28	30424.0
29	38229.5
30	45565.5
31	52674.5
32	59256.0
33	65098.0
34	70661.5
35	75742.0
36	79807.0
37	83258.5
38	86145.5
39	86897.0
40	87042.0
41	87611.0
42	87163.5
43	85919.5
44	83445.5
45	81482.0
46	80610.5
47	78796.5
48	76491.0
49	74305.0
50	72899.5
51	70413.0
52	64851.0
53	60445.5
54	58200.0
55	55398.5
56	50967.0
57	45912.5
58	41666.0
59	38002.5
60	33846.5
61	29128.5
62	24943.5
63	21323.0
64	17524.0
65	13935.5
66	11119.5
67	8559.5
68	6606.5
69	5065.5
70	3681.0
71	2709.5
72	2107.0
73	1564.5
74	1142.0
75	849.5
76	581.0
77	403.5
78	287.5
79	203.5
80	141.5
81	111.5
82	89.0
83	67.5
84	49.5
85	39.0
86	37.0
87	26.0
88	20.5
89	17.0
90	11.0
91	10.5
92	9.5
93	5.5
94	3.0
95	4.0
96	3.5
97	3.0
98	1.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0725471113863576
2	0.07526299870995352
3	0.07649749294795166
4	0.07900763123188122
5	0.07666209217968475
6	0.07662094237175147
7	0.07608599486861894
8	0.07575679640515277
9	0.07596254544481913
10-11	0.0756539218853196
12-13	0.07474862611078763
14-15	0.07291745965775705
16-17	0.07166239051579226
18-19	0.07123031753249291
20-21	0.0710451433967932
22-23	0.07285573494585713
24-25	0.07306148398552349
26-27	0.07238251215462452
28-29	0.06878190396046326
30-31	0.0736375812965893
32-33	0.07205331369115835
34-35	0.06960490011912869
36-37	0.06664211394793315
38-39	0.06841155568906382
40-41	0.07065422022142712
42-43	0.07522184890202024
44-45	0.0759419705408525
46-47	0.07908993084774776
48-49	0.08240249038637612
50-51	0.08328721125694145
52-53	0.08349296029660783
54-55	0.0834312355847079
56-57	0.08116799614837798
58-59	0.080489024317479
60-61	0.07791716132164953
62-63	0.07869900767238168
64-65	0.07779371189784971
66-67	0.07744393853041691
68-69	0.07713531497091738
70-71	0.07538644813375334
72-73	0.07553047246151978
74-75	0.07491322534252072
76-77	0.07509839947822045
78-79	0.07534529832582006
80-81	0.07637404352415185
82-83	0.07577737130911942
84-85	0.07491322534252072
86-87	0.0755921971734197
88-89	0.07281458513792387
90-91	0.07036617156589421
92-93	0.07034559666192758
94-95	0.07042789627779412
96-97	0.06981064915879506
98-99	0.06921397694376262
100	0.068514430208897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2430145.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.91710561353108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.62184824514092	56.36846278513594
2	12.61303729636972	17.637341215153764
3	3.3709670927814024	7.070647867372055
4	1.2793094838953336	3.577824651916079
5	0.6219690873118107	2.17431391829657
6	0.3881161309203082	1.6281573909522145
7	0.22945934212657138	1.1230193140232398
8	0.16450163870325032	0.9201182757451268
9	0.11822687804835653	0.7439473006978502
>10	0.5607966389318254	6.834913825718153
>50	0.025916326842762148	1.226457265830231
>100	0.005734059715765247	0.5942906797108082
>500	5.888960597965144E-5	0.03680947375931758
>1k	5.888960597965144E-5	0.06369603568866253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.114980793327147E-5	0.0
5	0.0	0.0	0.0	4.114980793327147E-5	0.0
6	0.0	0.0	0.0	4.114980793327147E-5	0.0
7	0.0	0.0	0.0	4.114980793327147E-5	0.0
8	0.0	0.0	0.0	4.114980793327147E-5	0.0
9	0.0	0.0	0.0	4.114980793327147E-5	0.0
10-11	0.0	0.0	0.0	4.114980793327147E-5	0.0
12-13	0.0	0.0	0.0	4.114980793327147E-5	0.0
14-15	0.0	0.0	0.0	1.0287451983317868E-4	0.0
16-17	0.0	0.0	0.0	1.2344942379981442E-4	0.0
18-19	0.0	0.0	0.0	1.6459923173308587E-4	0.0
20-21	0.0	0.0	0.0	2.0574903966635736E-4	0.0
22-23	0.0	0.0	0.0	2.0574903966635736E-4	0.0
24-25	0.0	0.0	0.0	2.263239436329931E-4	0.0
26-27	0.0	0.0	0.0	3.2919846346617175E-4	0.0
28-29	0.0	0.0	0.0	9.464455824652439E-4	0.0
30-31	4.114980793327147E-5	0.0	0.0	0.0034565838663948036	0.0
32-33	4.114980793327147E-5	0.0	0.0	0.009875953903985152	0.0
34-35	4.114980793327147E-5	0.0	0.0	0.01964903328813713	0.0
36-37	4.114980793327147E-5	0.0	0.0	0.0373640256034105	0.0
38-39	4.114980793327147E-5	0.0	0.0	0.07120974262852628	0.0
40-41	4.114980793327147E-5	0.0	0.0	0.11349117027996272	0.0
42-43	4.114980793327147E-5	0.0	0.0	0.15871480919862807	0.0
44-45	4.114980793327147E-5	0.0	0.0	0.20739503198368822	0.0
46-47	4.114980793327147E-5	0.0	0.0	0.26829674772493	0.0
48-49	4.114980793327147E-5	0.0	0.0	0.3413582317104535	0.0
50-51	4.114980793327147E-5	0.0	0.0	0.4231846247857638	0.0
52-53	4.114980793327147E-5	0.0	0.0	0.506204362291139	0.0
54-55	4.114980793327147E-5	0.0	0.0	0.594717599155606	0.0
56-57	4.114980793327147E-5	0.0	0.0	0.7032296426756428	0.0
58-59	4.114980793327147E-5	0.0	0.0	0.8300533507259855	0.0
60-61	4.114980793327147E-5	0.0	0.0	0.9695100498118425	0.0
62-63	4.114980793327147E-5	2.0574903966635734E-5	0.0	1.1080203033152345	0.0
64-65	4.114980793327147E-5	4.114980793327147E-5	0.0	1.2531556758958828	0.0
66-67	6.17247118999072E-5	4.114980793327147E-5	0.0	1.4182075555162346	0.0
68-69	8.229961586654294E-5	4.114980793327147E-5	0.0	1.6067354005625178	0.0
70-71	8.229961586654294E-5	4.114980793327147E-5	0.0	1.8099537270409791	0.0
72-73	8.229961586654294E-5	4.114980793327147E-5	0.0	2.03531065018754	0.0
74-75	8.229961586654294E-5	4.114980793327147E-5	0.0	2.256778916484407	0.0
76-77	8.229961586654294E-5	4.114980793327147E-5	0.0	2.4864565694639618	0.0
78-79	8.229961586654294E-5	4.114980793327147E-5	0.0	2.75378629670246	0.0
80-81	8.229961586654294E-5	4.114980793327147E-5	0.0	3.0494682416069825	0.0
82-83	8.229961586654294E-5	4.114980793327147E-5	0.0	3.3549438407996233	0.0
84-85	8.229961586654294E-5	4.114980793327147E-5	0.0	3.6555226128482046	0.0
86-87	8.229961586654294E-5	4.114980793327147E-5	0.0	3.9681788535252016	0.0
88	8.229961586654294E-5	4.114980793327147E-5	0.0	4.2229990391519845	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1955	0.0	35.581505	1
GTATCAA	3145	0.0	35.120167	1
TCAACGC	3100	0.0	33.50734	4
ATCAACG	3240	0.0	32.20456	3
CAACGCA	3300	0.0	31.475296	5
AACGCAG	3485	0.0	29.669579	6
TATCAAC	3810	0.0	27.63328	2
GTACATG	7020	0.0	27.450869	1
TACATGG	7035	0.0	26.25656	2
CGACCGT	75	1.1639731E-6	25.0663	20-21
ACATGGG	7090	0.0	24.793324	3
ACGCAGA	4175	0.0	24.540956	7
CGCAGAG	4360	0.0	23.499653	8
CATGGGG	4635	0.0	18.759937	4
GAGTACA	5135	0.0	18.39776	1
GCAGAGT	5390	0.0	18.223846	9
AGTACAT	5075	0.0	17.503912	2
GTCTAGG	620	0.0	17.435946	1
TCCGGTC	300	8.679308E-8	17.233082	8
TACACGG	220	3.196184E-5	17.091362	2
>>END_MODULE