FastQCFastQC Report
Wed 25 May 2016
SRR1032976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430145
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56600.23290791290231655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48150.19813632519870214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.10797709601690433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35700.055.5357741
GTATCAA71250.044.5089651
TCAACGC84950.036.877974
ATCAACG84950.036.82263
CAACGCA87000.036.0082635
AACGCAG90550.034.7004626
TATCAAC90600.034.5255552
TATAACG2600.030.7555052
ACGCAGA104200.030.1077657
CGCAGAG105600.029.6183078
GTACATG72300.026.9023761
GCAGAGT118800.026.2965099
GTGGTAT17050.025.9019281
ATAACGC3300.025.6575243
TAACGCA3150.025.3860154
TACATGG74000.025.2351572
ACATGGG74800.023.7709433
GAGTACT82850.022.14177712-13
AGAGTAC109200.021.78917110-11
CAGAGTA116550.021.20105210-11
Produced by FastQC (version 0.11.5)