FastQCFastQC Report
Wed 25 May 2016
SRR1032975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753211
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC23750.034.038564
GTATCAA25200.033.9451071
ATCAACG24900.032.844013
CAACGCA25750.031.3938875
AACGCAG26250.030.7959126
GGTATCA15750.030.1402681
GTACATG56800.029.3755721
TATCAAC29450.028.5676172
TACATGG56250.027.9080982
ACATGGG56600.026.7390353
ACGCAGA31350.025.3362927
CCGTATG950.001304927424.7366189
TACCTGG14800.024.4531782
CGCAGAG33400.023.6405038
GTACCTG16200.023.5004581
CATGGGG39750.022.2293644
CGTATAC1303.160146E-421.6927283
GAGTACA38650.020.9163191
TACCGTA1456.602191E-419.44817
GCAGAGT39100.019.3527669
Produced by FastQC (version 0.11.5)