##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032975_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1753211
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.590385298746128	33.0	31.0	34.0	30.0	34.0
2	31.63696611531641	34.0	31.0	34.0	30.0	34.0
3	31.794513609599758	34.0	31.0	34.0	30.0	34.0
4	35.141022957305196	37.0	35.0	37.0	33.0	37.0
5	35.18882268021362	37.0	35.0	37.0	33.0	37.0
6	35.246747254038446	37.0	36.0	37.0	33.0	37.0
7	35.21143034124244	37.0	37.0	37.0	33.0	37.0
8	35.194551597041084	37.0	37.0	37.0	33.0	37.0
9	36.84634251096987	39.0	38.0	39.0	34.0	39.0
10-11	36.83805885315573	39.0	38.0	39.0	34.0	39.0
12-13	36.775551545136324	39.0	38.0	39.0	34.0	39.0
14-15	38.25283893381915	41.0	39.0	41.0	34.0	41.0
16-17	38.271088020780155	41.0	39.0	41.0	34.0	41.0
18-19	38.279211686442764	41.0	39.0	41.0	34.0	41.0
20-21	38.31785563745608	41.0	39.0	41.0	34.0	41.0
22-23	38.30881964578137	41.0	39.0	41.0	34.0	41.0
24-25	38.337466511446706	41.0	39.0	41.0	34.0	41.0
26-27	38.31176966149539	41.0	39.0	41.0	34.0	41.0
28-29	38.2920538372164	41.0	39.0	41.0	34.0	41.0
30-31	38.2490293524282	41.0	39.0	41.0	33.5	41.0
32-33	38.20060905390167	41.0	39.0	41.0	33.0	41.0
34-35	38.12781519166832	41.0	39.0	41.0	33.0	41.0
36-37	38.036493610866	41.0	38.5	41.0	33.0	41.0
38-39	37.9422302848887	41.0	38.0	41.0	33.0	41.0
40-41	37.85348312325213	40.5	38.0	41.0	33.0	41.0
42-43	37.73856597979365	40.0	38.0	41.0	32.5	41.0
44-45	37.59530142122084	40.0	38.0	41.0	32.0	41.0
46-47	37.473950653971485	40.0	38.0	41.0	32.0	41.0
48-49	37.33617545178532	40.0	37.5	41.0	31.5	41.0
50-51	37.19272894135389	40.0	37.0	41.0	31.0	41.0
52-53	37.032159848415276	40.0	37.0	41.0	31.0	41.0
54-55	36.862918667519196	40.0	36.0	41.0	31.0	41.0
56-57	36.68153006112784	40.0	36.0	41.0	30.0	41.0
58-59	36.470938181428245	39.5	35.0	41.0	30.0	41.0
60-61	36.229664883462405	39.0	35.0	41.0	30.0	41.0
62-63	35.97319917568393	39.0	35.0	41.0	30.0	41.0
64-65	35.68496860902653	38.0	35.0	40.0	30.0	41.0
66-67	35.38339937406279	37.5	35.0	40.0	29.0	41.0
68-69	35.06725716414054	37.0	35.0	40.0	29.0	41.0
70-71	34.73053300486935	36.5	34.0	39.5	29.0	41.0
72-73	34.43577299024476	36.0	34.0	39.0	29.0	41.0
74-75	34.15739919496284	36.0	34.0	39.0	29.0	40.5
76-77	33.83726288507202	35.0	34.0	37.5	28.5	40.0
78-79	33.472441138003354	35.0	34.0	37.0	28.0	39.0
80-81	33.15456582236821	35.0	34.0	37.0	28.0	39.0
82-83	32.84342643298496	35.0	34.0	36.0	27.0	38.5
84-85	32.679541709469085	35.0	34.0	36.0	27.0	37.0
86-87	32.45424709290553	35.0	34.0	36.0	27.0	37.0
88-89	32.22792122568248	35.0	34.0	35.0	27.0	37.0
90-91	32.034285376945505	35.0	33.5	35.0	26.5	36.0
92-93	31.844565200651832	35.0	33.0	35.0	26.0	36.0
94-95	31.6637144644883	35.0	33.0	35.0	25.5	36.0
96-97	31.49612796177984	35.0	33.0	35.0	25.0	36.0
98-99	31.321307018949803	35.0	33.0	35.0	25.0	35.0
100	31.19743088538687	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3482321396593022
1101	2	0.38009391370711043
1101	3	0.4351510388627702
1101	4	0.40101808572597974
1101	5	0.3473815945649221
1101	6	0.40033904252305064
1101	7	0.3626223111146487
1101	8	0.3892226793194524
1101	9	0.3532024195342487
1101	10-11	0.3948399115372325
1101	12-13	0.2474292749785576
1101	14-15	0.3780077667191364
1101	16-17	0.40461308393364703
1101	18-19	0.36949815176588174
1101	20-21	0.3827660616083648
1101	22-23	0.38860622302405545
1101	24-25	0.40133312672280397
1101	26-27	0.43331862485382544
1101	28-29	0.46602101201264645
1101	30-31	0.43672342519889895
1101	32-33	0.46576101720602736
1101	34-35	0.40888274232586497
1101	36-37	0.4145293046438212
1101	38-39	0.4003943564365784
1101	40-41	0.43950478982871033
1101	42-43	0.36805435243761764
1101	44-45	0.39493191428498164
1101	46-47	0.38446094216262594
1101	48-49	0.3751426143637886
1101	50-51	0.45260393163718504
1101	52-53	0.36159174002720107
1101	54-55	0.3974643678511711
1101	56-57	0.387093438755322
1101	58-59	0.42274811080379493
1101	60-61	0.4506846392542414
1101	62-63	0.4396017309901623
1101	64-65	0.4225872235539896
1101	66-67	0.47261888212086944
1101	68-69	0.46020970787972004
1101	70-71	0.47216267081452656
1101	72-73	0.39638005532998477
1101	74-75	0.381326199153996
1101	76-77	0.48777457652985134
1101	78-79	0.44111439443263123
1101	80-81	0.49865392846592727
1101	82-83	0.5132515218886624
1101	84-85	0.43993555348946245
1101	86-87	0.4375670377576668
1101	88-89	0.43358383121675814
1101	90-91	0.4981566117645144
1101	92-93	0.6257094259226115
1101	94-95	0.617283388064422
1101	96-97	0.583880009320648
1101	98-99	0.5089673254621445
1101	100	0.5991982649373355
1102	1	0.11941978759384853
1102	2	0.24445205116873936
1102	3	0.21746882800943723
1102	4	0.16226306848230365
1102	5	0.13395077597915872
1102	6	0.22695954223652137
1102	7	0.22454031042261846
1102	8	0.18269716066883035
1102	9	0.2421103517618235
1102	10-11	0.22197007113081213
1102	12-13	0.26516763436902124
1102	14-15	0.34164201525036475
1102	16-17	0.3262603448330381
1102	18-19	0.2665202198940193
1102	20-21	0.29335109056000874
1102	22-23	0.30451404479269684
1102	24-25	0.2393073235851304
1102	26-27	0.2556878173990569
1102	28-29	0.3403972126577983
1102	30-31	0.3097237398962136
1102	32-33	0.28920365268458426
1102	34-35	0.3222638799234758
1102	36-37	0.2752890169876565
1102	38-39	0.3146604419542598
1102	40-41	0.33078554664086823
1102	42-43	0.42125666570969145
1102	44-45	0.3345596421652388
1102	46-47	0.3381494619397998
1102	48-49	0.35896506176261767
1102	50-51	0.38005658364713213
1102	52-53	0.39515568119497146
1102	54-55	0.44175159034013234
1102	56-57	0.439489180589149
1102	58-59	0.433880975194441
1102	60-61	0.44390749890825987
1102	62-63	0.45185594923624706
1102	64-65	0.5002070021357454
1102	66-67	0.46598734672644326
1102	68-69	0.5002645152514233
1102	70-71	0.40459491004848047
1102	72-73	0.39520500379808965
1102	74-75	0.46338875839376215
1102	76-77	0.3562527128954045
1102	78-79	0.27818338973463597
1102	80-81	0.31595962584896853
1102	82-83	0.3237343805861599
1102	84-85	0.3834086647396475
1102	86-87	0.4111979044415648
1102	88-89	0.4280854784844692
1102	90-91	0.40118379912787816
1102	92-93	0.5165212854331465
1102	94-95	0.4592714649676708
1102	96-97	0.5062836330543057
1102	98-99	0.4333831228249849
1102	100	0.4083116201366579
1103	1	0.10267585923743638
1103	2	0.09436583606539983
1103	3	0.15899632766585015
1103	4	0.1637946631705134
1103	5	0.11965858704606802
1103	6	-0.0538159333736985
1103	7	-0.047702254050619786
1103	8	-0.01512438800884297
1103	9	-0.0052510173053050835
1103	10-11	0.045889148550841696
1103	12-13	-0.006571465578623759
1103	14-15	0.08208456246573093
1103	16-17	0.08680864022242929
1103	18-19	0.10907961146666878
1103	20-21	0.14964071787086652
1103	22-23	0.05901862101548261
1103	24-25	0.09379031922132697
1103	26-27	0.16918893264351453
1103	28-29	0.1486366245175219
1103	30-31	0.17870450100127044
1103	32-33	0.2174819425979848
1103	34-35	0.15034604718193378
1103	36-37	0.1073901560235555
1103	38-39	0.04414820165343514
1103	40-41	0.15282407722730795
1103	42-43	0.09988823873482033
1103	44-45	0.10035203659237624
1103	46-47	0.09759826934015337
1103	48-49	0.10874055271683858
1103	50-51	0.07499271880496394
1103	52-53	0.14730693404866457
1103	54-55	0.1745010214161482
1103	56-57	0.1997992315823609
1103	58-59	0.16010714601138432
1103	60-61	0.23648374412707795
1103	62-63	0.19822334090392246
1103	64-65	0.12562329049593757
1103	66-67	0.0404417728338089
1103	68-69	0.14704261451981893
1103	70-71	0.15748283965352528
1103	72-73	0.13429208228988188
1103	74-75	0.0911046661403887
1103	76-77	0.044295269548150884
1103	78-79	0.19176815450046547
1103	80-81	0.1800673486136688
1103	82-83	0.16667761951872961
1103	84-85	0.13128801738144347
1103	86-87	0.10380455716884995
1103	88-89	0.2096726184347304
1103	90-91	0.17014959571302768
1103	92-93	0.18488657084877858
1103	94-95	0.2045030710236304
1103	96-97	0.1989641184459181
1103	98-99	0.1998881880757395
1103	100	0.22163641142945067
1104	1	-0.050220689289414366
1104	2	-0.04776967523131148
1104	3	-0.0026541879310464367
1104	4	-0.019610991759122953
1104	5	0.03176663831781923
1104	6	-0.010806633801450971
1104	7	0.005252671076895865
1104	8	8.01606482752959E-4
1104	9	0.027429313880190875
1104	10-11	0.07880893102782949
1104	12-13	0.04668882499240112
1104	14-15	0.1085168470281701
1104	16-17	0.0599682555991663
1104	18-19	0.04730732814682881
1104	20-21	0.054630198413732955
1104	22-23	0.020716821874508184
1104	24-25	0.07070144329099293
1104	26-27	0.05398021973875444
1104	28-29	0.05266467303916045
1104	30-31	0.0722787700085874
1104	32-33	0.15056859195456695
1104	34-35	0.09994190512400536
1104	36-37	0.11917167993983213
1104	38-39	0.08726220695999842
1104	40-41	0.05518377233178029
1104	42-43	0.04521505150803762
1104	44-45	0.016993026175093462
1104	46-47	0.018146170479020896
1104	48-49	-0.11514877016821146
1104	50-51	-0.015219865373559571
1104	52-53	0.008813495029819762
1104	54-55	0.014817207503469376
1104	56-57	-0.006827206426329724
1104	58-59	-0.049097986083410206
1104	60-61	-4.165277609118334E-4
1104	62-63	0.007576354079503744
1104	64-65	-0.016296738291828206
1104	66-67	-0.01922866902123843
1104	68-69	-0.15638656135458717
1104	70-71	-0.10984982276414712
1104	72-73	-0.09349327158553677
1104	74-75	-0.09254682649977752
1104	76-77	-0.06856836605570749
1104	78-79	-0.0022851590102632713
1104	80-81	0.01741747670472904
1104	82-83	-0.012790295585105582
1104	84-85	-0.049047977643141394
1104	86-87	-0.01927640275566489
1104	88-89	0.00487387894039415
1104	90-91	-0.016353078225421314
1104	92-93	-0.053273402482798105
1104	94-95	-0.011257128876014377
1104	96-97	0.006582864052294468
1104	98-99	-0.03666642873754
1104	100	-0.08124857846739175
1105	1	0.1390272556026524
1105	2	0.1983252494628367
1105	3	0.170290107227153
1105	4	0.15758058186766988
1105	5	0.1954169706077451
1105	6	0.20379487065331148
1105	7	0.15322437120023125
1105	8	0.21132523399041503
1105	9	0.19116714258488088
1105	10-11	0.19207409775307838
1105	12-13	0.1874147188234332
1105	14-15	0.27495353966973113
1105	16-17	0.24479899159361906
1105	18-19	0.2968380360638534
1105	20-21	0.26659245436447065
1105	22-23	0.19522758997818102
1105	24-25	0.23296845575236347
1105	26-27	0.28618248183086337
1105	28-29	0.3306636013214188
1105	30-31	0.3043744950153737
1105	32-33	0.3262205158027669
1105	34-35	0.3893202030512626
1105	36-37	0.35116585470356654
1105	38-39	0.3831642013040124
1105	40-41	0.3980536607806471
1105	42-43	0.4143401357176657
1105	44-45	0.3989067163265716
1105	46-47	0.2766610525764648
1105	48-49	0.24220449501967067
1105	50-51	0.20580839430613196
1105	52-53	0.2531966483280783
1105	54-55	0.2585030340211958
1105	56-57	0.21785640865351752
1105	58-59	0.2501399790794139
1105	60-61	0.23519990790413914
1105	62-63	0.22063004846827994
1105	64-65	0.26611184306638336
1105	66-67	0.2929299778459509
1105	68-69	0.30399448779844107
1105	70-71	0.3561314502830015
1105	72-73	0.3089079534247148
1105	74-75	0.4240749233257546
1105	76-77	0.38546050383214236
1105	78-79	0.31334765465380343
1105	80-81	0.30639282266314183
1105	82-83	0.36451849905884615
1105	84-85	0.26862180998084284
1105	86-87	0.2309066748030304
1105	88-89	0.27999162130924304
1105	90-91	0.3214049946411066
1105	92-93	0.2717330124097863
1105	94-95	0.2330902853858099
1105	96-97	0.2250707053752059
1105	98-99	0.19590183735552813
1105	100	0.21776943672706395
1106	1	0.08553468976118594
1106	2	0.10006938208308114
1106	3	0.17053505769672483
1106	4	0.13757139254933293
1106	5	0.1333911302445827
1106	6	0.1216855912796575
1106	7	0.19210489168322198
1106	8	0.19834227098661472
1106	9	0.1509132233406092
1106	10-11	0.24480505289082544
1106	12-13	0.1898298922258732
1106	14-15	0.31961450895903454
1106	16-17	0.3692464746393185
1106	18-19	0.27973377405800903
1106	20-21	0.3068545337234241
1106	22-23	0.33210601529658845
1106	24-25	0.4240996608395662
1106	26-27	0.3872894638749358
1106	28-29	0.2803605376026894
1106	30-31	0.283038735701993
1106	32-33	0.3097511254221459
1106	34-35	0.25416914903644994
1106	36-37	0.32326826046710266
1106	38-39	0.3145201403604574
1106	40-41	0.3584443905963113
1106	42-43	0.3374994233617059
1106	44-45	0.2680481031784936
1106	46-47	0.3117539565848446
1106	48-49	0.4033589674609104
1106	50-51	0.4295428597634796
1106	52-53	0.3534309391883781
1106	54-55	0.3946286703814934
1106	56-57	0.36020005240202124
1106	58-59	0.3093948356341514
1106	60-61	0.3526412341065637
1106	62-63	0.38762341378043885
1106	64-65	0.4003175735252995
1106	66-67	0.35213829969263344
1106	68-69	0.38955071723734847
1106	70-71	0.31899322455658563
1106	72-73	0.37009109653915573
1106	74-75	0.2914506377952577
1106	76-77	0.33857814548547793
1106	78-79	0.30615888767654553
1106	80-81	0.3553757121524015
1106	82-83	0.33573586319477045
1106	84-85	0.39199039353931653
1106	86-87	0.38868168353288723
1106	88-89	0.405820442687272
1106	90-91	0.3849474401025006
1106	92-93	0.427735658933468
1106	94-95	0.4579876039427191
1106	96-97	0.4533704241820651
1106	98-99	0.551384956577742
1106	100	0.6505367815654246
1107	1	-0.003248165243888934
1107	2	0.010613981516566895
1107	3	0.04928402889014194
1107	4	-0.03074029576234949
1107	5	-0.023934079525069762
1107	6	0.0412541872926937
1107	7	-0.032037484459152665
1107	8	-0.01724128948583825
1107	9	-0.07782623473217853
1107	10-11	-0.030826607629720115
1107	12-13	0.02169286053328534
1107	14-15	-0.014143601243524984
1107	16-17	-0.016030207526249285
1107	18-19	-0.013935190722065727
1107	20-21	0.0014619838864291523
1107	22-23	0.06489464230743636
1107	24-25	0.06409004082994585
1107	26-27	0.09393624918838839
1107	28-29	0.07695291641728375
1107	30-31	0.07240862507187984
1107	32-33	0.0195697347963133
1107	34-35	0.06579888555507551
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2216	48-49	-0.4562795405738811
2216	50-51	-0.49812896686190555
2216	52-53	-0.47778592650526974
2216	54-55	-0.4930974095241538
2216	56-57	-0.5250395171099882
2216	58-59	-0.5265845245309606
2216	60-61	-0.48850575100327376
2216	62-63	-0.45445649186603276
2216	64-65	-0.4618900042426475
2216	66-67	-0.4198950188979609
2216	68-69	-0.4606851665898688
2216	70-71	-0.46502800498786456
2216	72-73	-0.4749786366172657
2216	74-75	-0.4583341182124059
2216	76-77	-0.36153271991330627
2216	78-79	-0.2772711580259539
2216	80-81	-0.32422387505090455
2216	82-83	-0.3716021878150997
2216	84-85	-0.4335328262749272
2216	86-87	-0.33607697782640145
2216	88-89	-0.3396485238467122
2216	90-91	-0.5133017121899925
2216	92-93	-0.5042236511063365
2216	94-95	-0.4812227749456781
2216	96-97	-0.40612360500855615
2216	98-99	-0.3947783929726043
2216	100	-0.39264647068284475
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24784.0
3	3803.0
4	2654.0
5	2327.0
6	1812.0
7	1924.0
8	2114.0
9	2360.0
10	2538.0
11	2681.0
12	2835.0
13	3190.0
14	3433.0
15	3429.0
16	3655.0
17	3554.0
18	3508.0
19	3481.0
20	3559.0
21	3684.0
22	4367.0
23	6311.0
24	9448.0
25	12805.0
26	13667.0
27	11823.0
28	12276.0
29	15245.0
30	18912.0
31	22353.0
32	29061.0
33	39100.0
34	56637.0
35	87720.0
36	168194.0
37	357457.0
38	564578.0
39	241501.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5286349043981	19.00044981071417	12.635342792297049	23.835572492590682
2	15.792559221740307	19.630370029558065	40.39705309834537	24.180017650356262
3	18.75769568664791	25.0081487938358	29.040360362211747	27.193795157304546
4	12.478785584986959	16.237069410882903	38.12463715001567	33.159507854114466
5	13.021555218120998	37.80577469773602	34.779081848177285	14.393588235965705
6	31.492668012348613	37.30642502237729	17.692330526277335	13.508576438996766
7	28.366348191874923	31.886639186156764	21.757268435032344	17.98974418693597
8	25.521350659551683	36.6692811263412	19.79117431481748	18.018193899289646
9	25.140299110682594	17.515026033326997	20.5431235311478	36.8015513248426
10-11	24.266619248813292	27.002774001506445	28.08503132346901	20.645575426211245
12-13	24.799865739937346	24.173461489282026	28.377336992757265	22.649335778023364
14-15	22.06428120680458	26.206046809386308	25.246689284667433	26.482982699141676
16-17	22.019029939989874	29.662657809850156	25.702777202202675	22.615535047957298
18-19	21.973023266433774	28.53982578715437	27.636053839304065	21.85109710710779
20-21	22.948226206068277	27.93715096434945	27.2270568683195	21.88756596126277
22-23	22.680340576715384	27.77109596016102	27.205408904405164	22.34315455871843
24-25	22.425546149029287	27.734769447506274	27.334422843873675	22.505261559590767
26-27	22.241195663590325	27.71445157843303	27.41688281738765	22.627469940588995
28-29	22.323726295751396	27.79030524950376	27.42148290953821	22.464485545206635
30-31	22.510573174818006	28.263870138751336	26.944428112400743	22.281128574029918
32-33	22.13675794251739	27.94021699111064	27.539473556527344	22.38355150984463
34-35	22.256406977952164	27.573293955539015	27.5794871677784	22.59081189873042
36-37	22.61677468699789	27.969331929211723	27.106225224531034	22.307668159259357
38-39	22.44321806039978	28.120227621495786	27.23629500608895	22.200259312015486
40-41	22.16596238007958	27.831583129315696	27.535360362031657	22.467094128573066
42-43	22.099745548526307	27.854915565779915	27.47856142869579	22.566777456997986
44-45	22.261201299581657	27.960185969301765	27.484759199780566	22.293853531336012
46-47	22.230404581512282	28.17716254047414	27.32783364616586	22.264599231847722
48-49	22.143929174318842	27.920409024245853	27.488195624659966	22.44746617677534
50-51	22.111730395629753	27.830981187591448	27.531094736830084	22.52619367994871
52-53	22.1405915316706	27.80540243061423	27.5669138232621	22.487092214453067
54-55	22.258426016950796	28.24312736847033	27.300049438874485	22.19839717570439
56-57	22.000532625905656	27.9630598530512	27.43268890165745	22.6037186193857
58-59	21.903417645824742	27.56075109142884	27.789069869999018	22.746761392747402
60-61	22.35342069517082	28.022416357524577	27.298057692379054	22.326105254925558
62-63	22.08451250961537	27.97586987887844	27.38700244469467	22.55261516681152
64-65	22.060937362638615	27.661171640504495	27.63425594514336	22.643635051713527
66-67	21.941750343937848	27.799111755538686	27.41278820849769	22.84634969202578
68-69	22.37949440439143	27.887934270116233	27.406679680130246	22.32589164536209
70-71	22.364243788201616	27.68052425851585	27.46149334330399	22.49373860997855
72-73	22.02804188586877	27.84075081287447	27.732006018924448	22.39920128233231
74-75	22.102841309487214	27.846967902727197	27.555727187023976	22.49446360076161
76-77	22.144334793151792	27.822585248679083	27.391261411015773	22.641818547153353
78-79	22.211912250757788	27.949863284260278	27.4294301322632	22.40879433271873
80-81	22.12936809849571	27.731203247874177	27.587436179637763	22.551992473992346
82-83	22.019500519323127	27.78171817037281	27.713445517024066	22.485335793279994
84-85	22.100329629461548	27.777953785658365	27.522989721234914	22.598726863645172
86-87	22.25561643577565	27.834333875347106	27.470194237300422	22.439855451576822
88-89	21.91702431229149	27.739581659508044	27.607178808922338	22.73621521927813
90-91	21.94224389898633	27.668650320808563	27.629978055273085	22.759127724932025
92-93	22.16174516411858	27.807231446471953	27.548512932215463	22.482510457194003
94-95	21.990562230542093	27.57373863874417	27.681336288606005	22.754362842107724
96-97	22.006287396524094	27.545418091890745	27.706156254503995	22.74213825708117
98-99	21.975887123150677	27.688111433866823	27.631773470013304	22.704227972969193
100	22.214738476379107	27.477346606200538	27.47905898872396	22.82885592869639
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1908.0
1	1244.5
2	538.0
3	413.5
4	303.0
5	245.5
6	216.5
7	221.5
8	238.0
9	231.0
10	220.5
11	206.5
12	200.0
13	234.5
14	253.5
15	302.0
16	379.5
17	460.0
18	599.5
19	824.5
20	1179.5
21	1686.0
22	2406.5
23	3596.0
24	5189.5
25	7056.5
26	9494.5
27	12703.0
28	16486.5
29	20918.5
30	25694.0
31	30044.0
32	34307.0
33	38992.0
34	43173.0
35	46530.5
36	50296.0
37	53692.5
38	56447.5
39	58241.0
40	59603.0
41	60926.0
42	61329.5
43	61549.5
44	60876.5
45	60327.0
46	60525.0
47	59614.0
48	58702.5
49	58070.5
50	56591.0
51	54807.0
52	51816.5
53	49363.0
54	48059.5
55	45805.0
56	42433.5
57	38860.0
58	35793.5
59	33493.5
60	30627.5
61	26512.5
62	22901.5
63	19699.5
64	16040.5
65	12597.5
66	10080.0
67	7865.5
68	5800.0
69	4234.5
70	3087.0
71	2340.5
72	1872.5
73	1365.5
74	1001.5
75	702.5
76	426.5
77	295.0
78	215.0
79	143.5
80	92.5
81	70.5
82	64.5
83	47.5
84	30.0
85	27.0
86	22.0
87	15.5
88	14.5
89	14.5
90	10.0
91	5.0
92	6.0
93	4.0
94	2.5
95	2.5
96	1.5
97	1.5
98	1.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07774306686417094
2	0.08013867127231121
3	0.08088016787483082
4	0.08293354308180818
5	0.08162166447735042
6	0.08184981727812568
7	0.0812223970759937
8	0.08070905327424936
9	0.08088016787483082
10-11	0.08039534317318338
12-13	0.07911198366882252
14-15	0.07765750956388022
16-17	0.07643118825971318
18-19	0.07591784445796884
20-21	0.07588932535787193
22-23	0.07751491406339568
24-25	0.0772297230624266
26-27	0.07683045566106989
28-29	0.07320852994876258
30-31	0.0782564106659153
32-33	0.07654526466010081
34-35	0.07375039285060384
36-37	0.06992883343761817
38-39	0.07229591874566153
40-41	0.07529042425583686
42-43	0.08005311397202049
44-45	0.08079461057454008
46-47	0.08413134528587832
48-49	0.08726844629653818
50-51	0.08829513390002687
52-53	0.08869440130138358
54-55	0.08858032490099595
56-57	0.0862702777931464
58-59	0.0851865519894639
60-61	0.08296206218190509
62-63	0.08319021498268035
64-65	0.08242019928006383
66-67	0.08219204647928857
68-69	0.08187833637822259
70-71	0.08005311397202049
72-73	0.08002459487192358
74-75	0.07959680837046995
76-77	0.07956828927037306
78-79	0.07991051847153594
80-81	0.08093720607502462
82-83	0.08050941957357101
84-85	0.07959680837046995
86-87	0.0804523813733772
88-89	0.07760047136368639
90-91	0.0746915231538018
92-93	0.07454892765331726
94-95	0.07494819505467397
96-97	0.07377891195070074
98-99	0.0732370490488595
100	0.07221036144537081
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1753211.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.62527505114858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.1108921797655	57.99104344891143
2	11.515729461693446	16.266031212934298
3	2.9734026185887314	6.299921333269038
4	1.2023474506938954	3.3966447764921472
5	0.6142211727330757	2.168976963325626
6	0.38778127865335993	1.6432295678747784
7	0.24269147238508001	1.1998106392835153
8	0.1735505678105408	0.9805645269521965
9	0.12947614652816034	0.8229859615002725
>10	0.6223568099118137	7.543909765708801
>50	0.02245453809381817	1.0373619310706963
>100	0.00493459459406459	0.5242346760699875
>500	0.0	0.0
>1k	1.6170854856892858E-4	0.12528519660720452
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	1.140764003876316E-4	0.0
22-23	0.0	0.0	0.0	1.425955004845395E-4	0.0
24-25	0.0	0.0	0.0	1.711146005814474E-4	0.0
26-27	0.0	0.0	0.0	3.1371010106598694E-4	0.0
28-29	0.0	0.0	0.0	0.0013118786044577636	0.0
30-31	0.0	0.0	0.0	0.004534536915408356	0.0
32-33	0.0	0.0	0.0	0.012862114143705465	0.0
34-35	0.0	0.0	0.0	0.0234712193797552	0.0
36-37	0.0	0.0	0.0	0.03989822103557415	0.0
38-39	0.0	0.0	0.0	0.07024254353868417	0.0
40-41	0.0	0.0	0.0	0.11253636898239858	0.0
42-43	0.0	0.0	0.0	0.1574539516350285	0.0
44-45	0.0	0.0	0.0	0.2096153857122731	0.0
46-47	0.0	0.0	0.0	0.26776583080986827	0.0
48-49	0.0	0.0	0.0	0.3347857160376019	0.0
50-51	0.0	0.0	0.0	0.41626478501446773	0.0
52-53	0.0	0.0	0.0	0.49802904499230266	0.0
54-55	0.0	0.0	0.0	0.5865523316931048	0.0
56-57	0.0	0.0	0.0	0.687452907835965	0.0
58-59	0.0	0.0	0.0	0.8024419194266976	0.0
60-61	0.0	0.0	0.0	0.9252451644439831	0.0
62-63	0.0	0.0	0.0	1.0532674047790027	0.0
64-65	0.0	0.0	0.0	1.1913854065483276	0.0
66-67	0.0	0.0	0.0	1.34695709757696	0.0
68-69	0.0	0.0	0.0	1.519469134063156	0.0
70-71	0.0	0.0	0.0	1.7138553203236804	0.0
72-73	0.0	0.0	0.0	1.9283474721525247	0.0
74-75	0.0	0.0	0.0	2.141014401575167	0.0
76-77	2.85191000969079E-5	0.0	0.0	2.3557061871046896	0.0
78-79	5.70382001938158E-5	0.0	0.0	2.6046208927505017	0.0
80-81	5.70382001938158E-5	0.0	0.0	2.8790887120831434	0.0
82-83	5.70382001938158E-5	0.0	0.0	3.1732632295827488	0.0
84-85	5.70382001938158E-5	0.0	0.0	3.449584790421689	0.0
86-87	5.70382001938158E-5	0.0	0.0	3.748721631338156	0.0
88	5.70382001938158E-5	0.0	0.0	3.9928451281676876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2375	0.0	34.03856	4
GTATCAA	2520	0.0	33.945107	1
ATCAACG	2490	0.0	32.84401	3
CAACGCA	2575	0.0	31.393887	5
AACGCAG	2625	0.0	30.795912	6
GGTATCA	1575	0.0	30.140268	1
GTACATG	5680	0.0	29.375572	1
TATCAAC	2945	0.0	28.567617	2
TACATGG	5625	0.0	27.908098	2
ACATGGG	5660	0.0	26.739035	3
ACGCAGA	3135	0.0	25.336292	7
CCGTATG	95	0.0013049274	24.736618	9
TACCTGG	1480	0.0	24.453178	2
CGCAGAG	3340	0.0	23.640503	8
GTACCTG	1620	0.0	23.500458	1
CATGGGG	3975	0.0	22.229364	4
CGTATAC	130	3.160146E-4	21.692728	3
GAGTACA	3865	0.0	20.916319	1
TACCGTA	145	6.602191E-4	19.4481	7
GCAGAGT	3910	0.0	19.352766	9
>>END_MODULE