FastQCFastQC Report
Wed 25 May 2016
SRR1032975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753211
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41850.23870486781111916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32650.1862297236328086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.10780219836631186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.047.910921
GTATCAA50350.041.7137761
TATAACG1700.035.9830672
TAACGCA1850.035.6110574
ATCAACG60850.033.8720973
TCAACGC61400.033.645324
TATCAAC62850.033.1666952
CAACGCA62350.033.1270035
AACGCAG64600.032.2645266
ACGCAGA74150.027.9805817
GTACATG56500.027.5264991
ATAACGC2253.6379788E-1227.1887593
CGCAGAG76400.027.0333868
TACATGG58500.025.7393672
GTACACG1851.0075382E-825.3980121
ACATGGG57250.025.2343083
GCAGAGT81700.024.7829139
AGAGTAC75400.021.24493210-11
TACCTGG15200.021.0508212
GAGTACA40300.020.8698541
Produced by FastQC (version 0.11.5)