FastQCFastQC Report
Wed 25 May 2016
SRR1032974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054482
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG134550.029.4134481
TACATGG138300.027.460392
ACATGGG139850.026.2485963
GTATCAA85300.024.8510681
TCAACGC83300.024.6013554
ATCAACG84400.024.336413
CAACGCA85450.023.9270615
AACGCAG88050.023.2733356
CATGGGG89600.022.661744
TATCAAC98400.021.2560442
GAGTACA95650.020.5403961
GGTATCA61000.020.1107481
ACGCAGA101500.020.0041077
CGCAGAG105950.018.897758
AGTACAT97400.018.8201542
GTATAGA18350.018.6983951
GTATAGG16600.017.2718661
TATACTG24600.016.62255
TACCTGG27200.016.5889872
TCTATAC16600.016.422433
Produced by FastQC (version 0.11.5)