##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032974_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4054482
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.751195097178876	33.0	31.0	34.0	30.0	34.0
2	31.854420120745388	34.0	31.0	34.0	30.0	34.0
3	31.927668195345298	34.0	31.0	34.0	30.0	34.0
4	35.27377011416008	37.0	35.0	37.0	33.0	37.0
5	35.33941351817569	37.0	35.0	37.0	33.0	37.0
6	35.45270098621723	37.0	37.0	37.0	35.0	37.0
7	35.4180435379908	37.0	37.0	37.0	35.0	37.0
8	35.42806775316797	37.0	37.0	37.0	35.0	37.0
9	37.11263584349369	39.0	39.0	39.0	35.0	39.0
10-11	37.100140042550436	39.0	38.5	39.0	35.0	39.0
12-13	37.04877219827341	39.0	38.0	39.0	35.0	39.0
14-15	38.55284300189272	41.0	39.0	41.0	35.0	41.0
16-17	38.57673569644655	41.0	39.0	41.0	35.0	41.0
18-19	38.594599384089015	41.0	39.5	41.0	35.0	41.0
20-21	38.63349547488434	41.0	40.0	41.0	35.0	41.0
22-23	38.63001389573317	41.0	39.0	41.0	35.0	41.0
24-25	38.662241687100845	41.0	39.0	41.0	35.0	41.0
26-27	38.63933321198614	41.0	39.0	41.0	35.0	41.0
28-29	38.62113211996008	41.0	39.0	41.0	35.0	41.0
30-31	38.58077986287768	41.0	39.0	41.0	34.0	41.0
32-33	38.53016784881521	41.0	39.0	41.0	34.0	41.0
34-35	38.462772803036245	41.0	39.0	41.0	34.0	41.0
36-37	38.38215029688133	41.0	39.0	41.0	34.0	41.0
38-39	38.297444901716176	41.0	39.0	41.0	34.0	41.0
40-41	38.219648527234796	41.0	39.0	41.0	33.5	41.0
42-43	38.11561538563989	41.0	38.0	41.0	33.0	41.0
44-45	37.99898063427091	40.0	38.0	41.0	33.0	41.0
46-47	37.88491094546727	40.0	38.0	41.0	33.0	41.0
48-49	37.75734545128083	40.0	38.0	41.0	33.0	41.0
50-51	37.62677377776002	40.0	38.0	41.0	33.0	41.0
52-53	37.47768506556448	40.0	37.0	41.0	32.0	41.0
54-55	37.315863160818076	40.0	37.0	41.0	32.0	41.0
56-57	37.135382152393326	40.0	36.5	41.0	31.5	41.0
58-59	36.93140418430764	40.0	36.0	41.0	31.0	41.0
60-61	36.68902377665014	39.0	35.5	41.0	31.0	41.0
62-63	36.44278812928507	39.0	35.0	41.0	31.0	41.0
64-65	36.168270200730944	39.0	35.0	41.0	31.0	41.0
66-67	35.87072355975437	38.0	35.0	40.0	30.0	41.0
68-69	35.5575995897873	37.0	35.0	40.0	30.0	41.0
70-71	35.21555947220878	37.0	35.0	40.0	30.0	41.0
72-73	34.92104120822339	36.0	35.0	39.0	30.0	41.0
74-75	34.63646182175676	36.0	34.5	39.0	30.0	41.0
76-77	34.306090642405124	35.5	34.0	38.5	29.0	40.0
78-79	33.93536757593202	35.0	34.0	37.0	29.0	39.0
80-81	33.60769415180533	35.0	34.0	37.0	29.0	39.0
82-83	33.286850576719786	35.0	34.0	36.5	29.0	39.0
84-85	33.11368270965317	35.0	34.0	36.0	29.0	37.5
86-87	32.8826467844721	35.0	34.0	36.0	29.0	37.0
88-89	32.64551538272953	35.0	34.0	35.5	28.0	37.0
90-91	32.447252571351896	35.0	34.0	35.0	28.0	36.0
92-93	32.25773859644709	35.0	34.0	35.0	27.0	36.0
94-95	32.082753357888876	35.0	34.0	35.0	27.0	36.0
96-97	31.91512306627579	35.0	34.0	35.0	27.0	36.0
98-99	31.744432457709763	35.0	33.5	35.0	26.5	35.5
100	31.624557711687956	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4235790197933049
1101	2	0.4699433139608402
1101	3	0.4826397972850778
1101	4	0.44488955907416283
1101	5	0.3941028880195532
1101	6	0.391067026198229
1101	7	0.43259163961124614
1101	8	0.46274773737105335
1101	9	0.4901915615558181
1101	10-11	0.529474628531851
1101	12-13	0.3624591486245663
1101	14-15	0.44738066091609596
1101	16-17	0.397288782331664
1101	18-19	0.4492166261395454
1101	20-21	0.4156063257930285
1101	22-23	0.4198940441410457
1101	24-25	0.4167399693128431
1101	26-27	0.44292263533339593
1101	28-29	0.41900140653500983
1101	30-31	0.3870122142356678
1101	32-33	0.40137381353052604
1101	34-35	0.44855546417065995
1101	36-37	0.3919566112289843
1101	38-39	0.4802184468538826
1101	40-41	0.46565945025751887
1101	42-43	0.46909750309755793
1101	44-45	0.4622862415743114
1101	46-47	0.3656138584097164
1101	48-49	0.43499603369807716
1101	50-51	0.44651143329903675
1101	52-53	0.4448371597694063
1101	54-55	0.4642742802277766
1101	56-57	0.43764218290665013
1101	58-59	0.4401673048721122
1101	60-61	0.5009868450902033
1101	62-63	0.5584872615937968
1101	64-65	0.4817840909622291
1101	66-67	0.47985346256192685
1101	68-69	0.5416613367614005
1101	70-71	0.4877009807705619
1101	72-73	0.45413514892831586
1101	74-75	0.47589160381058804
1101	76-77	0.4618275567056003
1101	78-79	0.46228155887759215
1101	80-81	0.5144055946080783
1101	82-83	0.4511510163973256
1101	84-85	0.4448562819319122
1101	86-87	0.398142542219702
1101	88-89	0.395937024721313
1101	90-91	0.4649487650532933
1101	92-93	0.436421098272362
1101	94-95	0.4457570255294456
1101	96-97	0.4393211638132932
1101	98-99	0.4632695248818379
1101	100	0.47012293622379175
1102	1	0.20273971412832736
1102	2	0.2163500747030085
1102	3	0.22633002704907668
1102	4	0.22625926597509505
1102	5	0.12325559718829737
1102	6	0.08669374264103169
1102	7	0.13335196571818386
1102	8	0.10579074767400698
1102	9	0.16465296671097462
1102	10-11	0.1224275259081935
1102	12-13	0.22443228081614564
1102	14-15	0.25617696675159607
1102	16-17	0.22607933871052666
1102	18-19	0.19881909629067707
1102	20-21	0.18289997804105695
1102	22-23	0.2172784589447332
1102	24-25	0.18766365487678627
1102	26-27	0.20281375093402687
1102	28-29	0.1881955831206099
1102	30-31	0.19292220590686782
1102	32-33	0.22886595038360724
1102	34-35	0.25880195218638136
1102	36-37	0.1748544352591921
1102	38-39	0.23608632900911886
1102	40-41	0.22262728650062513
1102	42-43	0.2538271064740343
1102	44-45	0.24983680259983743
1102	46-47	0.22530502311929013
1102	48-49	0.22727985080881297
1102	50-51	0.2073671864213793
1102	52-53	0.25298746462614474
1102	54-55	0.25228242242767607
1102	56-57	0.1724272066913528
1102	58-59	0.14424517150402494
1102	60-61	0.21936594601045556
1102	62-63	0.22542849179679791
1102	64-65	0.19736133291659996
1102	66-67	0.2033417179328154
1102	68-69	0.24247019679796722
1102	70-71	0.20757263693511874
1102	72-73	0.17260808325342225
1102	74-75	0.23559929675332114
1102	76-77	0.17235651698355525
1102	78-79	0.18772739504635183
1102	80-81	0.16980334795947272
1102	82-83	0.17758953913951103
1102	84-85	0.23141650093562305
1102	86-87	0.16644590898896183
1102	88-89	0.1581061203548586
1102	90-91	0.10143887340716873
1102	92-93	0.12446329294517255
1102	94-95	0.1268381609112481
1102	96-97	0.10248058093242562
1102	98-99	0.13519924213849777
1102	100	0.13482354519796758
1103	1	0.15719027846441946
1103	2	0.19541112809680072
1103	3	0.19367302428515032
1103	4	0.17959770361601812
1103	5	0.09571644459102657
1103	6	0.05888177585946863
1103	7	0.13730757512128378
1103	8	0.17326665841051891
1103	9	0.18939691882285814
1103	10-11	0.16679713835961252
1103	12-13	0.2273090311530126
1103	14-15	0.1830243620205536
1103	16-17	0.23789400838063557
1103	18-19	0.21661600202826747
1103	20-21	0.1973381780719521
1103	22-23	0.18458535900425943
1103	24-25	0.18867944610759224
1103	26-27	0.2434267218811499
1103	28-29	0.2866541731658785
1103	30-31	0.25939022524775623
1103	32-33	0.2827293819581769
1103	34-35	0.2536611548794028
1103	36-37	0.2816531250183729
1103	38-39	0.24010430294967477
1103	40-41	0.26070804133102143
1103	42-43	0.2623673026346296
1103	44-45	0.28211916017759364
1103	46-47	0.291116456572027
1103	48-49	0.31275119357601966
1103	50-51	0.29750772432589656
1103	52-53	0.27740587156271346
1103	54-55	0.3475871463851874
1103	56-57	0.36353339558907294
1103	58-59	0.3135190618323307
1103	60-61	0.32265297093513823
1103	62-63	0.3910069275430672
1103	64-65	0.3346834974858339
1103	66-67	0.28439368353938477
1103	68-69	0.3611275205274538
1103	70-71	0.27591270459173245
1103	72-73	0.30674049718332697
1103	74-75	0.280129244783204
1103	76-77	0.33937090724482744
1103	78-79	0.3160319549882331
1103	80-81	0.30324162316146897
1103	82-83	0.31712403374534404
1103	84-85	0.32422616907777524
1103	86-87	0.30453110277437645
1103	88-89	0.30787688774582733
1103	90-91	0.3241853415593212
1103	92-93	0.3336008638482397
1103	94-95	0.28265269476307253
1103	96-97	0.30754108451077755
1103	98-99	0.3743599158887747
1103	100	0.40063212947618965
1104	1	0.07495798535105891
1104	2	0.12705393479780014
1104	3	0.10570271487408078
1104	4	0.06710063987777204
1104	5	0.09920612331809764
1104	6	0.09729345616503338
1104	7	0.11061285382930208
1104	8	0.18658481866338406
1104	9	0.24195011776763664
1104	10-11	0.2558599132720474
1104	12-13	0.24830031312072975
1104	14-15	0.27231077486442246
1104	16-17	0.22276639710072033
1104	18-19	0.2655773208700509
1104	20-21	0.24826834500735373
1104	22-23	0.24060603510737621
1104	24-25	0.24466383029354688
1104	26-27	0.25996499024778075
1104	28-29	0.26872082125553476
1104	30-31	0.22884513084891012
1104	32-33	0.27787933571031687
1104	34-35	0.32775780132084975
1104	36-37	0.3250055118399686
1104	38-39	0.33017300823019013
1104	40-41	0.31889659169928564
1104	42-43	0.3027119099153026
1104	44-45	0.2695381476266121
1104	46-47	0.2639022927313164
1104	48-49	0.25327003913893975
1104	50-51	0.25292664985154545
1104	52-53	0.24879940516809995
1104	54-55	0.21586996674640346
1104	56-57	0.19816498767052337
1104	58-59	0.20833016124474568
1104	60-61	0.26648995450300816
1104	62-63	0.18525748137719233
1104	64-65	0.19398677981396162
1104	66-67	0.2415019676816499
1104	68-69	0.2587677208847978
1104	70-71	0.21383011957640718
1104	72-73	0.2587688862690243
1104	74-75	0.27785133602080236
1104	76-77	0.20046014123602163
1104	78-79	0.22038361078030277
1104	80-81	0.21134455612310887
1104	82-83	0.2065666640574335
1104	84-85	0.2125538078709539
1104	86-87	0.1909031031755788
1104	88-89	0.182253830151339
1104	90-91	0.22803910747376932
1104	92-93	0.24962813460522426
1104	94-95	0.266035678034676
1104	96-97	0.24127148757076
1104	98-99	0.23617540537172488
1104	100	0.193915594100055
1105	1	0.009964128291642282
1105	2	0.07505551186840265
1105	3	-0.028620186935828684
1105	4	0.07534120848502823
1105	5	0.1334304172312244
1105	6	0.15901343971516013
1105	7	0.14738829480718607
1105	8	0.2104592061843391
1105	9	0.18072346644544268
1105	10-11	0.17397545793284053
1105	12-13	0.18743174628773573
1105	14-15	0.2738737059300007
1105	16-17	0.19082448196463275
1105	18-19	0.20539152092241864
1105	20-21	0.17837585583536963
1105	22-23	0.21433697220207648
1105	24-25	0.22935615749612026
1105	26-27	0.2031625207997152
1105	28-29	0.25235786480319433
1105	30-31	0.2061381479437543
1105	32-33	0.23759105427699012
1105	34-35	0.2045076201708511
1105	36-37	0.22189811014692395
1105	38-39	0.2574440051196092
1105	40-41	0.259054678155735
1105	42-43	0.2286950672977639
1105	44-45	0.21046603376651518
1105	46-47	0.247125484053349
1105	48-49	0.24302789182171836
1105	50-51	0.23627984879429675
1105	52-53	0.2921249351167532
1105	54-55	0.21309697826084317
1105	56-57	0.22023815049099937
1105	58-59	0.23546510242192653
1105	60-61	0.25365122624862124
1105	62-63	0.22367407462365918
1105	64-65	0.2638631680646739
1105	66-67	0.24323960838027858
1105	68-69	0.24876029572539693
1105	70-71	0.2600087327753684
1105	72-73	0.224152110443967
1105	74-75	0.28941677414618994
1105	76-77	0.26595151676289674
1105	78-79	0.26421017212469167
1105	80-81	0.26581590491426255
1105	82-83	0.27491126920663334
1105	84-85	0.301643981695328
1105	86-87	0.30436793478219215
1105	88-89	0.2411335233649723
1105	90-91	0.21896533082762204
1105	92-93	0.18371614446576956
1105	94-95	0.22691565017481707
1105	96-97	0.19600485982733673
1105	98-99	0.2463666152289079
1105	100	0.25904809472886825
1106	1	-0.09224212018708755
1106	2	0.02688796718476283
1106	3	-0.032806768249663776
1106	4	0.003995840111706173
1106	5	-0.09824519647215624
1106	6	0.02216365915636942
1106	7	-0.028684181529960995
1106	8	-0.02975432347949436
1106	9	-0.018803999637171387
1106	10-11	0.02467531473872242
1106	12-13	0.03398070350259985
1106	14-15	0.04826940828202453
1106	16-17	0.04211399105977165
1106	18-19	0.020373945379503766
1106	20-21	0.04389338932779907
1106	22-23	0.06185000340086333
1106	24-25	0.10320569309418204
1106	26-27	0.1182903587064672
1106	28-29	0.11380161906108555
1106	30-31	0.10505179939823961
1106	32-33	0.11199464387499347
1106	34-35	0.0907786782276574
1106	36-37	0.12687551302032318
1106	38-39	0.1111083650912974
1106	40-41	0.10764469666239052
1106	42-43	0.08527481592044239
1106	44-45	0.07683072311137096
1106	46-47	0.0908885970169564
1106	48-49	0.11493342708872234
1106	50-51	0.03445910197778801
1106	52-53	0.06410432014740763
1106	54-55	0.08751133077914375
1106	56-57	0.0820564654993774
1106	58-59	0.05129498865424864
1106	60-61	0.10517668525518786
1106	62-63	0.08115674048164578
1106	64-65	0.1200436040013173
1106	66-67	0.1053649586340697
1106	68-69	0.13321762094113154
1106	70-71	0.1266870208002402
1106	72-73	0.13391314468995574
1106	74-75	0.11445624374947982
1106	76-77	0.07827091106690887
1106	78-79	0.11131599226700928
1106	80-81	0.08255802512167776
1106	82-83	0.02487466971542318
1106	84-85	0.05096141230870188
1106	86-87	0.06200849574003087
1106	88-89	0.019224939994444412
1106	90-91	-0.012953185578076898
1106	92-93	0.0011518472805747137
1106	94-95	0.011317336376784226
1106	96-97	0.034763715458030475
1106	98-99	0.02644119030770753
1106	100	0.006682477158619804
1107	1	0.1648704228753779
1107	2	0.20330557191494592
1107	3	0.18382841256883253
1107	4	0.2245445582288923
1107	5	0.2418250877233703
1107	6	0.2504154859787775
1107	7	0.22602016991738338
1107	8	0.23016579187896014
1107	9	0.21782418976017226
1107	10-11	0.1538370894837655
1107	12-13	0.17579327196425254
1107	14-15	0.2301246875651657
1107	16-17	0.18663164200605564
1107	18-19	0.2291530801549868
1107	20-21	0.26408958608399047
1107	22-23	0.23941955533645398
1107	24-25	0.21134041786491764
1107	26-27	0.20944164993456127
1107	28-29	0.19755142357028177
1107	30-31	0.14287654671141325
1107	32-33	0.10181349313342025
1107	34-35	0.15172083176611295
1107	36-37	0.13963634507174305
1107	38-39	0.11413590773965154
1107	40-41	0.12017096632138191
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2216	50-51	-0.3335635818048459
2216	52-53	-0.3593353116226581
2216	54-55	-0.37051577833950233
2216	56-57	-0.4103516344397562
2216	58-59	-0.3965923069581976
2216	60-61	-0.36738415436923333
2216	62-63	-0.32264867679430154
2216	64-65	-0.4010371968620632
2216	66-67	-0.36560599594414356
2216	68-69	-0.3863959317815855
2216	70-71	-0.4116880325486747
2216	72-73	-0.4006735236792025
2216	74-75	-0.35268412773387325
2216	76-77	-0.3407758750546819
2216	78-79	-0.293268756415884
2216	80-81	-0.3236354993698427
2216	82-83	-0.3333012916144895
2216	84-85	-0.35352140496865303
2216	86-87	-0.34686909124492615
2216	88-89	-0.30394860314295613
2216	90-91	-0.3135000119299889
2216	92-93	-0.34528302419226975
2216	94-95	-0.3915585854224588
2216	96-97	-0.3867221217175931
2216	98-99	-0.4373943253751271
2216	100	-0.4712674553243943
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43883.0
3	6527.0
4	4471.0
5	3822.0
6	3118.0
7	3681.0
8	4350.0
9	4723.0
10	5026.0
11	5177.0
12	5892.0
13	6420.0
14	6934.0
15	7443.0
16	7392.0
17	7428.0
18	7201.0
19	7025.0
20	7280.0
21	7718.0
22	8964.0
23	12671.0
24	19727.0
25	26828.0
26	28986.0
27	24538.0
28	25672.0
29	32209.0
30	39834.0
31	48076.0
32	62335.0
33	83904.0
34	122749.0
35	192323.0
36	372443.0
37	811783.0
38	1347951.0
39	636540.0
40	1438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62166114098293	19.507037595053937	13.12465073663716	23.746650527325972
2	15.53885522191365	19.954370010685505	40.44158356747727	24.06519119992358
3	18.464767111412364	25.333151989186494	29.687386222798068	26.514694676603074
4	12.221575766073164	16.565185119177357	38.836704765341764	32.37653434940771
5	12.898100295492037	38.41359455905632	35.491257054375616	13.197048091076022
6	31.109930667593467	37.937968131974465	17.809033749933352	13.143067450498716
7	27.841116378631543	32.467105149482556	22.243030655784075	17.44874781610183
8	25.392589191717608	36.473701294293484	20.103301577663164	18.030407936325744
9	25.265857004698315	16.976341552878406	20.832792346438296	36.925009095984976
10-11	24.08039332982249	26.976531848822706	28.691839186188282	20.251235635166523
12-13	24.628968640625615	24.40153594820044	28.680252318479326	22.28924309269462
14-15	21.84123255825147	26.363224270455106	25.634682121159504	26.16086105013392
16-17	21.726445198936034	29.748048872987276	26.234162886015476	22.29134304206121
18-19	21.68071570169256	28.830747498836534	28.041958654703752	21.44657814476716
20-21	22.68689518176773	27.993196552712707	27.626885563049807	21.693022702469754
22-23	22.489799499900588	27.702424436820454	27.676309971788726	22.131466091490225
24-25	22.26758396670766	27.90670838904991	27.58537363480244	22.240334009439987
26-27	22.050562075086834	27.873933459313978	27.74436147756735	22.33114298803184
28-29	22.00490177325226	27.969034417309725	27.860176054121894	22.165887755316117
30-31	22.202102753276375	28.221249433831396	27.443114711004096	22.133533101888133
32-33	22.01143079427989	28.080925610239067	27.80204839063396	22.105595204847088
34-35	22.23433144989661	27.782631204496553	27.806054353305743	22.176982992301088
36-37	22.412760750247923	28.079035973772815	27.415717552794227	22.092485723185035
38-39	22.152121413985572	28.246144664653727	27.617853624428022	21.983880296932675
40-41	22.025782479672397	27.90472492855135	27.84865942607585	22.2208331657004
42-43	21.99948164786612	27.933181941598995	27.735183768173176	22.33215264236171
44-45	22.071757076577807	27.993617775443248	27.77820288641635	22.156422261562597
46-47	22.126210222669616	28.1123291025629	27.640421869145126	22.121038805622355
48-49	22.062130644930363	27.92957506578655	27.728142326054435	22.28015196322865
50-51	21.92595768809737	27.965234246220106	27.7821779905185	22.32663007516403
52-53	22.122866620381085	27.873769960657256	27.699686800894856	22.303676618066806
54-55	22.073779217773666	28.23037877034637	27.563965131528196	22.13187688035177
56-57	22.017296110010637	27.95269297794028	27.673887099973303	22.35612381207578
58-59	22.032666778983018	27.549028864685333	27.963167642029273	22.455136714302377
60-61	22.23342617944346	28.01634252809327	27.57960212759445	22.17062916486882
62-63	21.90464224364454	28.050444284214528	27.691765962178884	22.353147509962053
64-65	21.9833882290853	27.80622159535156	27.79937171136144	22.411018464201703
66-67	21.825091359105308	27.810737282080655	27.708433844085317	22.65573751472872
68-69	22.13225427750225	27.94626574473316	27.647798329184763	22.273681648579828
70-71	22.16070403323784	27.772650462100184	27.69959958393458	22.367045920727392
72-73	21.877543554916475	27.81779448958709	27.86411302957932	22.440548925917117
74-75	21.97431987330499	27.93327777920393	27.68149464962392	22.410907697867156
76-77	22.043522733892363	27.795035578632877	27.58705336646831	22.57438832100645
78-79	22.01868366239079	27.89301737827684	27.684121133164012	22.404177826168358
80-81	22.043949002293374	27.754779337985568	27.77516815217855	22.426103507542503
82-83	21.954178537951393	27.732641697937744	27.906945172097373	22.406234592013487
84-85	21.935600042159233	27.792253216060892	27.752858521953534	22.519288219826343
86-87	22.141128139963318	27.83307125447222	27.67498469303944	22.350815912525025
88-89	21.807730437906596	27.776641682302206	27.757635908866575	22.657991970924623
90-91	21.893596424846194	27.660965593636753	27.809514853445055	22.635923128071997
92-93	22.13270180197469	27.74776920556506	27.718409908361725	22.40111908409853
94-95	21.913011606145105	27.55516914341355	27.82060055275585	22.7112186976855
96-97	21.956103515329733	27.47577503893876	27.956997740003253	22.611123705728257
98-99	21.977983599199373	27.651030631208318	27.759371335140287	22.611614434452022
100	22.009952120138244	27.33708345766696	27.821509483046075	22.831454939148717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3376.0
1	1973.5
2	552.0
3	460.0
4	359.0
5	321.5
6	296.5
7	284.5
8	292.0
9	317.5
10	328.0
11	328.5
12	351.5
13	378.5
14	412.0
15	500.0
16	628.5
17	817.5
18	1131.5
19	1564.0
20	2269.5
21	3435.5
22	5072.5
23	7613.5
24	11420.5
25	16145.5
26	22424.0
27	30580.0
28	40320.0
29	51878.0
30	63237.5
31	73831.0
32	84408.5
33	94911.0
34	105239.5
35	114049.5
36	121681.0
37	128887.0
38	134662.0
39	137979.0
40	140623.0
41	143931.0
42	145580.5
43	145724.0
44	144530.0
45	142698.5
46	141855.0
47	140472.0
48	139747.5
49	136122.0
50	130172.0
51	125658.0
52	117384.0
53	110064.5
54	106512.0
55	101296.0
56	93056.0
57	83944.5
58	75860.0
59	70626.0
60	64494.0
61	55813.0
62	48567.0
63	42100.5
64	34761.5
65	27044.0
66	21448.5
67	16966.0
68	12547.5
69	9031.0
70	6342.0
71	4458.0
72	3453.0
73	2586.0
74	1911.5
75	1304.5
76	796.0
77	551.0
78	383.5
79	253.0
80	171.5
81	126.5
82	94.0
83	74.5
84	59.0
85	51.0
86	50.0
87	34.5
88	30.0
89	29.5
90	20.5
91	13.0
92	8.0
93	6.0
94	3.0
95	3.5
96	4.0
97	2.0
98	1.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07640926757104853
2	0.07890033794699298
3	0.08023219735591378
4	0.08225465053242313
5	0.08072547861847704
6	0.0807501426816052
7	0.07991156453524766
8	0.07983757234586317
9	0.08001022078776031
10-11	0.0795539356198893
12-13	0.07827140433722483
14-15	0.07654491991825342
16-17	0.07501574800430733
18-19	0.07442381048923143
20-21	0.07415250579482163
22-23	0.07615029490820283
24-25	0.07644626366574078
26-27	0.07562001755094733
28-29	0.07120515025100617
30-31	0.07712452540176526
32-33	0.07541037301435793
34-35	0.0723766932495939
36-37	0.0692566892638813
38-39	0.0704528963255972
40-41	0.07327693155377185
42-43	0.07848104887381421
44-45	0.07966492390396603
46-47	0.0830685646156525
48-49	0.08637354907482632
50-51	0.08734777956838875
52-53	0.08742177175777326
54-55	0.08742177175777326
56-57	0.08522667013936674
58-59	0.0844620841823937
60-61	0.08222998646929497
62-63	0.08284658804749903
64-65	0.08184769349080845
66-67	0.08135441222824519
68-69	0.08104611143914316
70-71	0.0788263457576085
72-73	0.07864136528414728
74-75	0.07838239262130156
76-77	0.07828373636878891
78-79	0.07864136528414728
80-81	0.07993622859837582
82-83	0.0792826309254795
84-85	0.07830840043191707
86-87	0.07943061530424848
88-89	0.07600231052943385
90-91	0.07310428311187471
92-93	0.07286997451215717
94-95	0.0731166151434388
96-97	0.07220404480769677
98-99	0.07180941979764616
100	0.07110649399849352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4054482.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.377954956921116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.61681511130826	40.21370716349097
2	15.296928015732467	16.942251812689946
3	5.2753771361662904	8.764187922821652
4	2.3708771413395273	5.25177310165977
5	1.223661368988612	3.38819320871879
6	0.7614393790782464	2.530017338221267
7	0.49005300797580686	1.8996693381531558
8	0.3510395454365742	1.5551881708227737
9	0.2579991404249277	1.2858718299638412
>10	1.2534990113307858	12.33093639632094
>50	0.0721972923055669	2.7197339700570766
>100	0.029089159584663682	2.6048018763138345
>500	8.910350961412038E-4	0.35734706203159655
>1k	1.3365523247686135E-4	0.15632080873433263
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.699609469224428E-5	0.0
12-13	0.0	0.0	0.0	4.932812625632571E-5	0.0
14-15	0.0	0.0	0.0	7.399218938448858E-5	0.0
16-17	0.0	0.0	0.0	7.399218938448858E-5	0.0
18-19	0.0	0.0	0.0	7.399218938448858E-5	0.0
20-21	0.0	0.0	0.0	7.399218938448858E-5	0.0
22-23	0.0	0.0	0.0	7.399218938448858E-5	0.0
24-25	0.0	0.0	0.0	9.865625251265143E-5	0.0
26-27	0.0	0.0	0.0	1.7264844189714E-4	0.0
28-29	0.0	0.0	0.0	8.015820516652929E-4	0.0
30-31	0.0	0.0	0.0	0.004291546984300338	0.0
32-33	0.0	0.0	0.0	0.012110054995927964	0.0
34-35	0.0	1.2332031564081428E-5	0.0	0.02356651231895961	0.0
36-37	0.0	2.4664063128162856E-5	0.0	0.044814602703871914	0.0
38-39	0.0	2.4664063128162856E-5	0.0	0.08351251775195945	0.0
40-41	0.0	2.4664063128162856E-5	0.0	0.12976996814882888	0.0
42-43	0.0	2.4664063128162856E-5	0.0	0.17542314899905834	0.0
44-45	0.0	2.4664063128162856E-5	0.0	0.23118859573183453	0.0
46-47	0.0	2.4664063128162856E-5	0.0	0.30431754290683743	0.0
48-49	0.0	2.4664063128162856E-5	0.0	0.38383448243203444	0.0
50-51	0.0	2.4664063128162856E-5	0.0	0.4740432933232901	0.0
52-53	0.0	2.4664063128162856E-5	0.0	0.5633518659103678	0.0
54-55	0.0	2.4664063128162856E-5	0.0	0.662427407496198	0.0
56-57	0.0	2.4664063128162856E-5	0.0	0.7826030550881715	0.0
58-59	0.0	2.4664063128162856E-5	0.0	0.916701566315993	0.0
60-61	0.0	2.4664063128162856E-5	0.0	1.06495724977938	0.0
62-63	0.0	2.4664063128162856E-5	0.0	1.2173564958482983	0.0
64-65	0.0	2.4664063128162856E-5	0.0	1.373911636554312	0.0
66-67	0.0	2.4664063128162856E-5	0.0	1.55202316843434	0.0
68-69	0.0	2.4664063128162856E-5	0.0	1.7556620056520167	0.0
70-71	0.0	2.4664063128162856E-5	0.0	1.9766273472172278	0.0
72-73	0.0	2.4664063128162856E-5	0.0	2.212292470406824	0.0
74-75	0.0	2.4664063128162856E-5	0.0	2.446453085745602	0.0
76-77	0.0	2.4664063128162856E-5	0.0	2.6954861311506626	0.0
78-79	0.0	2.4664063128162856E-5	0.0	2.984314642413014	0.0
80-81	0.0	2.4664063128162856E-5	0.0	3.3040225607118243	0.0
82-83	0.0	2.4664063128162856E-5	0.0	3.6320299362532626	0.0
84-85	0.0	2.4664063128162856E-5	0.0	3.9534520069394805	0.0
86-87	0.0	2.4664063128162856E-5	0.0	4.289253226429418	0.0
88	0.0	2.4664063128162856E-5	0.0	4.563665592793358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	13455	0.0	29.413448	1
TACATGG	13830	0.0	27.46039	2
ACATGGG	13985	0.0	26.248596	3
GTATCAA	8530	0.0	24.851068	1
TCAACGC	8330	0.0	24.601355	4
ATCAACG	8440	0.0	24.33641	3
CAACGCA	8545	0.0	23.927061	5
AACGCAG	8805	0.0	23.273335	6
CATGGGG	8960	0.0	22.66174	4
TATCAAC	9840	0.0	21.256044	2
GAGTACA	9565	0.0	20.540396	1
GGTATCA	6100	0.0	20.110748	1
ACGCAGA	10150	0.0	20.004107	7
CGCAGAG	10595	0.0	18.89775	8
AGTACAT	9740	0.0	18.820154	2
GTATAGA	1835	0.0	18.698395	1
GTATAGG	1660	0.0	17.271866	1
TATACTG	2460	0.0	16.6225	5
TACCTGG	2720	0.0	16.588987	2
TCTATAC	1660	0.0	16.42243	3
>>END_MODULE