FastQCFastQC Report
Wed 25 May 2016
SRR1032974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4054482
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79050.1949694190281274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65330.16113032441628794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88600.036.056611
GTATCAA144450.034.279371
ATCAACG160550.030.2917043
TCAACGC160750.030.224764
CAACGCA163900.029.6126145
AACGCAG168600.028.8429016
TATCAAC172050.028.4036642
GTACATG135250.026.7810021
TACATGG134600.025.6835942
ACGCAGA192350.025.231757
ACATGGG133700.024.4493273
CGCAGAG197750.024.4000518
GCAGAGT212200.022.4691949
GAGTACT120150.020.93668612-13
AGAGTAC170400.020.57913410-11
GAGTACA97800.020.271381
CATGGGG89300.019.9618724
GTACTTT127700.019.6068814-15
TACACCG7500.019.4391125
CGACGCG1352.6175258E-819.1377390-91
Produced by FastQC (version 0.11.5)