##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032974_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4054482 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.848908442558134 34.0 31.0 34.0 31.0 34.0 2 32.905403945559506 34.0 31.0 34.0 31.0 34.0 3 32.992277928475204 34.0 33.0 34.0 31.0 34.0 4 36.39330523603262 37.0 37.0 37.0 35.0 37.0 5 36.36931622831227 37.0 37.0 37.0 35.0 37.0 6 36.42638023796874 37.0 37.0 37.0 35.0 37.0 7 36.399616523146484 37.0 37.0 37.0 35.0 37.0 8 36.33541720002703 37.0 37.0 37.0 35.0 37.0 9 38.18017295427628 39.0 39.0 39.0 37.0 39.0 10-11 38.19546085048596 39.0 39.0 39.0 37.0 39.0 12-13 38.1863080166591 39.0 39.0 39.0 37.0 39.0 14-15 39.75108164248849 41.0 40.0 41.0 37.0 41.0 16-17 39.76328480432272 41.0 40.0 41.0 37.0 41.0 18-19 39.78533410679835 41.0 40.0 41.0 37.5 41.0 20-21 39.79638866321271 41.0 40.0 41.0 37.0 41.0 22-23 39.75991026720553 41.0 40.0 41.0 37.0 41.0 24-25 39.74093016074556 41.0 40.0 41.0 37.0 41.0 26-27 39.668491190736574 41.0 40.0 41.0 37.0 41.0 28-29 39.60993562186242 41.0 40.0 41.0 37.0 41.0 30-31 39.50617785453234 41.0 40.0 41.0 36.5 41.0 32-33 39.387314335098786 41.0 39.5 41.0 36.0 41.0 34-35 39.29844687928076 41.0 39.0 41.0 35.0 41.0 36-37 39.212039047158186 41.0 39.0 41.0 35.0 41.0 38-39 39.120191679233 41.0 39.0 41.0 35.0 41.0 40-41 39.024955099073075 41.0 39.0 41.0 35.0 41.0 42-43 38.88806954871177 41.0 39.0 41.0 35.0 41.0 44-45 38.72264138797509 41.0 39.0 41.0 35.0 41.0 46-47 38.50620954785346 41.0 39.0 41.0 35.0 41.0 48-49 38.30767456853921 41.0 38.0 41.0 35.0 41.0 50-51 38.15527174618114 41.0 38.0 41.0 34.0 41.0 52-53 38.013838389219636 40.0 38.0 41.0 34.0 41.0 54-55 37.86551007009034 40.0 37.5 41.0 34.0 41.0 56-57 37.65622883515083 40.0 37.0 41.0 33.5 41.0 58-59 37.4839510941225 40.0 37.0 41.0 33.0 41.0 60-61 37.2895337801475 40.0 36.0 41.0 33.0 41.0 62-63 37.051075328488324 39.0 35.5 41.0 33.0 41.0 64-65 36.763363236043475 39.0 35.0 41.0 32.5 41.0 66-67 36.48484886602037 38.5 35.0 41.0 32.0 41.0 68-69 36.18945959064561 37.5 35.0 40.5 32.0 41.0 70-71 35.863872746259574 37.0 35.0 40.0 32.0 41.0 72-73 35.52221282521417 37.0 35.0 39.0 31.0 41.0 74-75 35.177289725296596 36.0 35.0 39.0 31.0 41.0 76-77 34.82823329835969 36.0 35.0 39.0 31.0 40.5 78-79 34.48492063844407 35.0 35.0 37.0 31.0 39.5 80-81 34.155071967269805 35.0 34.5 37.0 31.0 39.0 82-83 33.84813682734317 35.0 34.0 36.5 30.0 39.0 84-85 33.570363365776444 35.0 34.0 36.0 30.0 37.0 86-87 33.42922314120521 35.0 34.0 36.0 30.0 37.0 88-89 33.24280117657447 35.0 34.0 35.5 30.0 37.0 90-91 33.070972444815396 35.0 34.0 35.0 30.0 36.0 92-93 32.91183300357481 35.0 34.0 35.0 30.0 36.0 94-95 32.815531059208055 35.0 34.0 35.0 30.0 36.0 96-97 32.754891870280844 35.0 34.0 35.0 30.0 36.0 98-99 32.650937283726996 35.0 34.0 35.0 30.0 36.0 100 32.571784015812625 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17872637558009785 1101 2 0.21642046596198128 1101 3 0.19843795163426847 1101 4 0.0864125042591084 1101 5 0.12112488865029292 1101 6 0.13738052781780397 1101 7 0.09186748824681246 1101 8 0.0694776916232911 1101 9 0.15702211512792985 1101 10-11 0.2014512125843737 1101 12-13 0.22264605885083455 1101 14-15 0.31762045925491833 1101 16-17 0.3017443206370274 1101 18-19 0.2722097500838174 1101 20-21 0.3002595791503424 1101 22-23 0.3032383936335279 1101 24-25 0.3104842277139923 1101 26-27 0.3465354759911463 1101 28-29 0.3167207419778322 1101 30-31 0.2958137857233183 1101 32-33 0.3027720663314497 1101 34-35 0.3715861497994126 1101 36-37 0.35514474919008876 1101 38-39 0.33406298309881777 1101 40-41 0.4316654931700157 1101 42-43 0.4479667082479253 1101 44-45 0.47582350350060665 1101 46-47 0.501904392909708 1101 48-49 0.4965938812355333 1101 50-51 0.5340643673091918 1101 52-53 0.5891723485405294 1101 54-55 0.5836547310732954 1101 56-57 0.5727339956920758 1101 58-59 0.5930168775367974 1101 60-61 0.6161986159738646 1101 62-63 0.5987515469556257 1101 64-65 0.5896399315435801 1101 66-67 0.5795728397029407 1101 68-69 0.5544864128727696 1101 70-71 0.5770489403195924 1101 72-73 0.48781170650957506 1101 74-75 0.5802188032398732 1101 76-77 0.5756655864830904 1101 78-79 0.549645559458618 1101 80-81 0.5474663943873423 1101 82-83 0.5177961904484931 1101 84-85 0.5634863650689894 1101 86-87 0.5551316913745836 1101 88-89 0.5122013260155072 1101 90-91 0.5773443394524946 1101 92-93 0.5838602648672051 1101 94-95 0.5705882402797613 1101 96-97 0.5513498889769792 1101 98-99 0.5289155208257554 1101 100 0.5003107019806876 1102 1 0.13139853805827784 1102 2 0.1906612710584099 1102 3 0.16994439346949974 1102 4 0.13221326857608773 1102 5 0.1237581794896272 1102 6 0.11545137188444698 1102 7 0.12525612789826823 1102 8 -0.03740203055179592 1102 9 0.05086855363766318 1102 10-11 0.12122421617814183 1102 12-13 0.1270107377324905 1102 14-15 0.1386973381631691 1102 16-17 0.16698918047288913 1102 18-19 0.17923361255358827 1102 20-21 0.22224050004502516 1102 22-23 0.2053764159149054 1102 24-25 0.19307017846252705 1102 26-27 0.2207852793739704 1102 28-29 0.21151306159642047 1102 30-31 0.27064064315410974 1102 32-33 0.24679045459836857 1102 34-35 0.21429808887913282 1102 36-37 0.26411202246697485 1102 38-39 0.2983285938935296 1102 40-41 0.2613687475514084 1102 42-43 0.30520552331633866 1102 44-45 0.24110975750748764 1102 46-47 0.22062247834270465 1102 48-49 0.17475057354075574 1102 50-51 0.19945240908287332 1102 52-53 0.26761615429336416 1102 54-55 0.20621479728754366 1102 56-57 0.23741225441516178 1102 58-59 0.17875473394030195 1102 60-61 0.20914064626793305 1102 62-63 0.22243460204943943 1102 64-65 0.21955805637873027 1102 66-67 0.20081535348121093 1102 68-69 0.25899273347585705 1102 70-71 0.2498231239929254 1102 72-73 0.23773063978718767 1102 74-75 0.26145462768724315 1102 76-77 0.25142542827754255 1102 78-79 0.24237177463286486 1102 80-81 0.25782695767390607 1102 82-83 0.24909087854337741 1102 84-85 0.2100555125359449 1102 86-87 0.2242017230968827 1102 88-89 0.18984644400531892 1102 90-91 0.20935476267862896 1102 92-93 0.22076840506139916 1102 94-95 0.22090434059974484 1102 96-97 0.2842687865900402 1102 98-99 0.23630161823027862 1102 100 0.23435390621398966 1103 1 0.1488759175426111 1103 2 0.15991825279536442 1103 3 0.16811781418449812 1103 4 0.09607775411625141 1103 5 0.11018021099489061 1103 6 0.10321833806445113 1103 7 0.10764584145580613 1103 8 0.021381379126985678 1103 9 0.061871143148032104 1103 10-11 0.07799880407988269 1103 12-13 0.1424402444118229 1103 14-15 0.16611439496394098 1103 16-17 0.17958007846269197 1103 18-19 0.16735175339492514 1103 20-21 0.19311480539109738 1103 22-23 0.17863835240233072 1103 24-25 0.1379524885823784 1103 26-27 0.16646396811778175 1103 28-29 0.1655803285458859 1103 30-31 0.1761821501966807 1103 32-33 0.18721138594936093 1103 34-35 0.19315950378769742 1103 36-37 0.2034253413143361 1103 38-39 0.23788628424733815 1103 40-41 0.18113378519028345 1103 42-43 0.1621210586709978 1103 44-45 0.09309461808668118 1103 46-47 0.09879321540353914 1103 48-49 0.09822377419218498 1103 50-51 0.07601794215754154 1103 52-53 0.06084693108301309 1103 54-55 0.0477306098135557 1103 56-57 0.08814766528411866 1103 58-59 0.05123163616337223 1103 60-61 0.014261623657127132 1103 62-63 0.0924693430058312 1103 64-65 0.08525227132451363 1103 66-67 0.12271382093934591 1103 68-69 0.08516457998104698 1103 70-71 0.06623857590046356 1103 72-73 0.0806289234719415 1103 74-75 0.05723381280239437 1103 76-77 0.08059278433976402 1103 78-79 0.0668017156559344 1103 80-81 0.09855793874708496 1103 82-83 0.08896594600273744 1103 84-85 0.10020285771046389 1103 86-87 0.11378021471445265 1103 88-89 0.06184057307024915 1103 90-91 0.05092337161872962 1103 92-93 0.09207932105039873 1103 94-95 0.08557530608521091 1103 96-97 0.03268790362856322 1103 98-99 0.06352244181638866 1103 100 0.07145554271310317 1104 1 0.11305194826550036 1104 2 0.1456226673200689 1104 3 0.13525068598475798 1104 4 0.06277892359663184 1104 5 0.05760532032950749 1104 6 0.06457898739220269 1104 7 0.0561160467651618 1104 8 0.04118721783365231 1104 9 0.09363041770273384 1104 10-11 0.11836338813743907 1104 12-13 0.13341931598022683 1104 14-15 0.1692637732365796 1104 16-17 0.1549976116394305 1104 18-19 0.16123116559749917 1104 20-21 0.17090693307634552 1104 22-23 0.19023498725632493 1104 24-25 0.18237184849864008 1104 26-27 0.17544489664578578 1104 28-29 0.18074933022430173 1104 30-31 0.19228417396004716 1104 32-33 0.2129894002966708 1104 34-35 0.13631648899450965 1104 36-37 0.14929569193968462 1104 38-39 0.12057458553161382 1104 40-41 0.1411977980139767 1104 42-43 0.18073635563528967 1104 44-45 0.15972921994070788 1104 46-47 0.17806834299071994 1104 48-49 0.19664031566795614 1104 50-51 0.18423969754426395 1104 52-53 0.19642180710185642 1104 54-55 0.21058769122406318 1104 56-57 0.21128238588641324 1104 58-59 0.1669826897413671 1104 60-61 0.17937005537724815 1104 62-63 0.1194192664637228 1104 64-65 0.13931040291093666 1104 66-67 0.14690161895716614 1104 68-69 0.17363801680057378 1104 70-71 0.18992476678426584 1104 72-73 0.1941229257111985 1104 74-75 0.23119571101705105 1104 76-77 0.19596395470820482 1104 78-79 0.25374875059240054 1104 80-81 0.2512490191671901 1104 82-83 0.19185167717038354 1104 84-85 0.1493524959129502 1104 86-87 0.15319791808776984 1104 88-89 0.19157958829950417 1104 90-91 0.17230713467988323 1104 92-93 0.18431349040488954 1104 94-95 0.14694229606975284 1104 96-97 0.16824702070508124 1104 98-99 0.1628894792631641 1104 100 0.1804988024929628 1105 1 0.004170551281653445 1105 2 0.049692792862757074 1105 3 0.04881736909192824 1105 4 0.01690277352246028 1105 5 0.003537138607129009 1105 6 0.05194438954520564 1105 7 0.06718721588174503 1105 8 -0.02131808083316855 1105 9 0.052537529321583065 1105 10-11 0.0914307038058908 1105 12-13 0.07295862939479036 1105 14-15 0.10442553889991046 1105 16-17 0.08534040083891625 1105 18-19 0.0708569872128777 1105 20-21 0.10385690054663144 1105 22-23 0.14035813881201165 1105 24-25 0.12068245669893685 1105 26-27 0.096287205510869 1105 28-29 0.08787956759051951 1105 30-31 0.16371794870453016 1105 32-33 0.16406446759266657 1105 34-35 0.16742136114498862 1105 36-37 0.11005429240779563 1105 38-39 0.10497056795948367 1105 40-41 0.14525751357919603 1105 42-43 0.08993942560673673 1105 44-45 0.07080497342869307 1105 46-47 0.1369021222317457 1105 48-49 0.16585734484540637 1105 50-51 0.16995352574146239 1105 52-53 0.15000902795871696 1105 54-55 0.14800481539109 1105 56-57 0.2525804585534388 1105 58-59 0.20680173177986916 1105 60-61 0.19630152072267748 1105 62-63 0.2327441078784247 1105 64-65 0.2041875136538991 1105 66-67 0.1992802578239221 1105 68-69 0.14421478341707683 1105 70-71 0.15169427091336019 1105 72-73 0.15876451217771148 1105 74-75 0.22279884637580238 1105 76-77 0.22158453105326714 1105 78-79 0.2999031831350365 1105 80-81 0.2659231353495599 1105 82-83 0.2644787760864773 1105 84-85 0.2521167430619755 1105 86-87 0.23161195158050418 1105 88-89 0.197571941357225 1105 90-91 0.22572729823245652 1105 92-93 0.20646118585367645 1105 94-95 0.1890490448265325 1105 96-97 0.2540919806600428 1105 98-99 0.2199531934775436 1105 100 0.26580616732368156 1106 1 0.0929057952345076 1106 2 0.09831427135760862 1106 3 0.07785339189085505 1106 4 0.07125902518278338 1106 5 0.045099698818297895 1106 6 0.056098487420030096 1106 7 0.0766852288297315 1106 8 0.03972275084966981 1106 9 0.11713001345475504 1106 10-11 0.077285834787574 1106 12-13 0.07396586630452617 1106 14-15 0.1427782458605762 1106 16-17 0.11908385242234942 1106 18-19 0.12319770822952592 1106 20-21 0.09340451971079489 1106 22-23 0.11622400045303749 1106 24-25 0.12682730547072651 1106 26-27 0.11146603796707666 1106 28-29 0.061316902809380736 1106 30-31 0.07428661913957058 1106 32-33 0.0876216188662653 1106 34-35 0.11103411583405887 1106 36-37 0.11576080943111577 1106 38-39 0.14507765873823075 1106 40-41 0.167118812409484 1106 42-43 0.13734847642729875 1106 44-45 0.17820320459419747 1106 46-47 0.1870501833041729 1106 48-49 0.13818100983169046 1106 50-51 0.09573189902423707 1106 52-53 0.12959349246477814 1106 54-55 0.10458619374795575 1106 56-57 0.073172227828465 1106 58-59 0.07373799615768917 1106 60-61 0.03686126043688631 1106 62-63 0.09668499869891889 1106 64-65 0.04070926710103606 1106 66-67 0.01581429490577335 1106 68-69 0.026774692000664402 1106 70-71 0.08342542907255535 1106 72-73 0.07908093980927333 1106 74-75 0.0918878338670055 1106 76-77 0.0407439141616166 1106 78-79 0.02919938493106855 1106 80-81 0.05797258560073004 1106 82-83 0.056790680287917894 1106 84-85 0.039602214510154 1106 86-87 0.09755227038098724 1106 88-89 0.11912176628039362 1106 90-91 0.12200367267128343 1106 92-93 0.03918485213039702 1106 94-95 0.0729574889187461 1106 96-97 0.02395493614572075 1106 98-99 0.07522517775733917 1106 100 0.1110407133275686 1107 1 0.04790920642288654 1107 2 0.04768839776220801 1107 3 0.01920933847409856 1107 4 0.038289732682500244 1107 5 0.01612788107112806 1107 6 0.04014989265209579 1107 7 0.04957832164371467 1107 8 0.03990992233606505 1107 9 0.06541794986173954 1107 10-11 0.02942734557457527 1107 12-13 -0.0022686123099262545 1107 14-15 0.07532145079140662 1107 16-17 0.08878782697510701 1107 18-19 0.0705965667864632 1107 20-21 0.07607542226860886 1107 22-23 0.08791831332104749 1107 24-25 0.07951472866317744 1107 26-27 0.12959394992959972 1107 28-29 0.0947453262747402 1107 30-31 0.157291246171809 1107 32-33 0.118908839864595 1107 34-35 0.18277905696780294 1107 36-37 0.18577910556351895 1107 38-39 0.2478246248962961 1107 40-41 0.25926462160216346 1107 42-43 0.31282094754701717 1107 44-45 0.35216203728627704 1107 46-47 0.32583837205510946 1107 48-49 0.3370144865338105 1107 50-51 0.3732912657690335 1107 52-53 0.3515239890629971 1107 54-55 0.3718378125075503 1107 56-57 0.38937219374317067 1107 58-59 0.39245010536080116 1107 60-61 0.40801383916665657 1107 62-63 0.45062019281770915 1107 64-65 0.3794673042850647 1107 66-67 0.3941622909801126 1107 68-69 0.34440196675086554 1107 70-71 0.3948221685144304 1107 72-73 0.37533026456196694 1107 74-75 0.3125879211428213 1107 76-77 0.30906755614769565 1107 78-79 0.3334483637274772 1107 80-81 0.2815875989972412 1107 82-83 0.32283296711629816 1107 84-85 0.30424996478968325 1107 86-87 0.2800408814133277 1107 88-89 0.3013473484815208 1107 90-91 0.26806568772516215 1107 92-93 0.3054155065140165 1107 94-95 0.3321854790988681 1107 96-97 0.32446433921379736 1107 98-99 0.2571371507042812 1107 100 0.2539428739382501 1108 1 0.0024927909773708734 1108 2 -0.006046947325820895 1108 3 0.01429615848817889 1108 4 0.0019920957357513203 1108 5 -0.04009258218507483 1108 6 -0.04528287911955431 1108 7 -0.028118079525043527 1108 8 -0.035842529936566336 1108 9 -0.019514137569785817 1108 10-11 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24-25 -0.15387430105152333 2214 26-27 -0.16318874995927501 2214 28-29 -0.17091221705297244 2214 30-31 -0.11964291255472403 2214 32-33 -0.1326255954420219 2214 34-35 -0.13724019224497397 2214 36-37 -0.1846662209667045 2214 38-39 -0.14452303191619364 2214 40-41 -0.20308455667244374 2214 42-43 -0.17352589963027043 2214 44-45 -0.15521270698582157 2214 46-47 -0.14971954267237209 2214 48-49 -0.14671320434856483 2214 50-51 -0.13074244276772617 2214 52-53 -0.13668929819176867 2214 54-55 -0.14499607914804358 2214 56-57 -0.13968229905297846 2214 58-59 -0.14044376993908259 2214 60-61 -0.11386064292491938 2214 62-63 -0.13588097408940314 2214 64-65 -0.14028548528857954 2214 66-67 -0.16007260375371857 2214 68-69 -0.19736318741943393 2214 70-71 -0.17902926342594583 2214 72-73 -0.1614302301305841 2214 74-75 -0.14742982019231476 2214 76-77 -0.09538333186998216 2214 78-79 -0.14325662476646528 2214 80-81 -0.14209090247124578 2214 82-83 -0.1092156137127418 2214 84-85 -0.20244365349176974 2214 86-87 -0.1705926988859119 2214 88-89 -0.18346818782028862 2214 90-91 -0.19179060202642972 2214 92-93 -0.20064466731302133 2214 94-95 -0.17935311305962642 2214 96-97 -0.18016953179338202 2214 98-99 -0.1777923594120807 2214 100 -0.18320036357013691 2215 1 -0.1770155142794323 2215 2 -0.21148917338067008 2215 3 -0.22505673655334135 2215 4 -0.1236268450605209 2215 5 -0.12287653668713006 2215 6 -0.11862074172503867 2215 7 -0.1358858175322979 2215 8 -0.08235150666203594 2215 9 -0.14160834672065192 2215 10-11 -0.17452864333817786 2215 12-13 -0.17992254085174864 2215 14-15 -0.2663732129628329 2215 16-17 -0.2835683556424584 2215 18-19 -0.19625941351992537 2215 20-21 -0.24100805032416162 2215 22-23 -0.2506514829826543 2215 24-25 -0.2675739834285551 2215 26-27 -0.27547689330589265 2215 28-29 -0.24782020398209426 2215 30-31 -0.2889038559086572 2215 32-33 -0.29220685309478256 2215 34-35 -0.28043977474536064 2215 36-37 -0.2728187547074725 2215 38-39 -0.2836871025526122 2215 40-41 -0.28140107108441725 2215 42-43 -0.3250491327667433 2215 44-45 -0.3267465682167625 2215 46-47 -0.32383010687627944 2215 48-49 -0.3713694685962281 2215 50-51 -0.30193580851903334 2215 52-53 -0.37088759373209257 2215 54-55 -0.39045741011483415 2215 56-57 -0.3809316567165695 2215 58-59 -0.4109723460946455 2215 60-61 -0.3255168949739158 2215 62-63 -0.2935755924920187 2215 64-65 -0.3695640176051498 2215 66-67 -0.3687680470916419 2215 68-69 -0.33402550068442594 2215 70-71 -0.39491235591172114 2215 72-73 -0.3943204085209331 2215 74-75 -0.3624162720403348 2215 76-77 -0.3513693735375938 2215 78-79 -0.3524918975364315 2215 80-81 -0.3531445019186421 2215 82-83 -0.4027642688852069 2215 84-85 -0.45461192962095254 2215 86-87 -0.5455214891281912 2215 88-89 -0.5416699279610953 2215 90-91 -0.48018901528395475 2215 92-93 -0.47688443376942047 2215 94-95 -0.4716572366687899 2215 96-97 -0.45346019264128046 2215 98-99 -0.48634671746450664 2215 100 -0.5063920245567957 2216 1 -0.1788614355896172 2216 2 -0.1966361666282168 2216 3 -0.20622790485503373 2216 4 -0.12470211232097483 2216 5 -0.15562129861395846 2216 6 -0.12478865251853932 2216 7 -0.15240978121035909 2216 8 -0.13858768034947389 2216 9 -0.17687955961401514 2216 10-11 -0.20317097183399113 2216 12-13 -0.23516665851584406 2216 14-15 -0.32617603237236636 2216 16-17 -0.28424103108461907 2216 18-19 -0.26001854902067834 2216 20-21 -0.2523240908736355 2216 22-23 -0.28660478189740246 2216 24-25 -0.276032393011711 2216 26-27 -0.27265929349583473 2216 28-29 -0.2943302544807054 2216 30-31 -0.32626447075247 2216 32-33 -0.3986694597962668 2216 34-35 -0.4003063761653465 2216 36-37 -0.3821453988080563 2216 38-39 -0.33850285788913936 2216 40-41 -0.2903828536508115 2216 42-43 -0.3167754600927495 2216 44-45 -0.30581092499643603 2216 46-47 -0.3268347238512277 2216 48-49 -0.4204283020897819 2216 50-51 -0.41469354980628026 2216 52-53 -0.3651607133535819 2216 54-55 -0.34444814720931305 2216 56-57 -0.4083978437815148 2216 58-59 -0.3657523774246272 2216 60-61 -0.41952825571471664 2216 62-63 -0.33348046601383174 2216 64-65 -0.4146529982512064 2216 66-67 -0.47954616445459664 2216 68-69 -0.4417013482156662 2216 70-71 -0.4509985032495365 2216 72-73 -0.38473428579540325 2216 74-75 -0.4209119258005103 2216 76-77 -0.4422547932204779 2216 78-79 -0.4200218521340062 2216 80-81 -0.421384774275289 2216 82-83 -0.4166817916732839 2216 84-85 -0.45350003349057744 2216 86-87 -0.46680355024773235 2216 88-89 -0.41007622465204463 2216 90-91 -0.3708231725014315 2216 92-93 -0.35103447749241923 2216 94-95 -0.40868851743414325 2216 96-97 -0.36763060664885217 2216 98-99 -0.3451306245053516 2216 100 -0.34724284226374635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17.0 3 7.0 4 6.0 5 34.0 6 95.0 7 218.0 8 464.0 9 795.0 10 1537.0 11 2423.0 12 3819.0 13 5968.0 14 7139.0 15 9503.0 16 11975.0 17 17335.0 18 20990.0 19 11965.0 20 8001.0 21 7617.0 22 7729.0 23 8364.0 24 9433.0 25 11076.0 26 13575.0 27 17798.0 28 22795.0 29 28983.0 30 37141.0 31 47064.0 32 60499.0 33 79160.0 34 110713.0 35 172173.0 36 333975.0 37 789620.0 38 1441120.0 39 750370.0 40 2986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32573348710608 19.64025859866202 13.412758467942032 23.621249446289873 2 15.690462584070936 20.088381617984922 40.11813648317915 24.10301931476499 3 18.42702199421627 25.42898895664667 30.114330462020362 26.029658587116707 4 12.279337709105773 16.543557468952187 38.725931422112886 32.45117339982916 5 12.478634628845581 38.12679771254259 35.64017681278238 13.754390845829455 6 30.765548103179384 38.161430586554594 17.467393559045536 13.605627751220483 7 27.43740347045154 32.68873422785487 22.277009652091596 17.596852649601992 8 25.59880738655687 35.929949766888626 19.966223116616444 18.50501972993806 9 25.47729226816863 16.18960870987171 21.032553495753046 37.30054552620662 10-11 24.168229135065836 26.565243106271037 28.595206490002916 20.671321268660215 12-13 25.093877659743708 23.96427506468772 28.910853897627785 22.030993377940792 14-15 22.002131966349264 25.7387951529089 26.463537924657533 25.795534956084303 16-17 21.287096600749493 29.345573614582577 26.974691218261665 22.392638566406266 18-19 21.18167862792695 27.894836277321026 29.626760830864047 21.296724263887977 20-21 22.167233693477982 27.038287505037633 29.462271629273477 21.3322071722109 22-23 21.877638291160547 26.766426495589684 29.56772905762552 21.78820615562425 24-25 21.68990608371715 27.016087461172404 29.436001619943656 21.858004835166785 26-27 21.469760306050528 27.033476743132024 29.576942121597614 21.919820829219834 28-29 21.438277520223078 27.152427571220144 29.71365179673693 21.695643111819848 30-31 21.649598211290343 27.355550446585386 29.266908279294462 21.72794306282981 32-33 21.491258418886673 27.200603390804705 29.565680729545367 21.742457460763255 34-35 21.693042003173627 27.0147120645146 29.517556584483973 21.774689347827803 36-37 21.8824849297503 27.292637644985962 29.089039290163882 21.735838135099854 38-39 21.654967545301606 27.496751569190714 29.18319669279394 21.665084192713742 40-41 21.60208810507352 27.187626047664693 29.31306806663349 21.897217780628296 42-43 21.65757028500515 27.25605937983681 29.079025602408525 22.007344732749516 44-45 21.721200063109755 27.3270549496838 29.01371007585763 21.938034911348815 46-47 21.885058793883676 27.532632299973564 28.642827449911827 21.939481456230933 48-49 21.908316933872932 27.521969515522493 28.45697333040324 22.11274022020134 50-51 21.79407720981133 27.62346883142281 28.387843198065703 22.194610760700154 52-53 21.988020637283725 27.531250551690924 28.316265842248555 22.164462968776792 54-55 21.922138370603577 27.85193141657793 28.197700510826117 22.028229701992377 56-57 21.853116985588997 27.56614219027897 28.304764133754812 22.275976690377224 58-59 21.858070335815416 27.168681881727586 28.593004732190224 22.380243050266778 60-61 21.990883805738928 27.565372286567012 28.299849643401448 22.14389426429261 62-63 21.684298982892937 27.5333342302858 28.441190015635364 22.341176771185904 64-65 21.777833189726774 27.36709425535528 28.48356281402759 22.371509740890357 66-67 21.66005690246706 27.381355667796242 28.383543000504503 22.575044429232197 68-69 21.9671943134508 27.48782827884217 28.291408516011916 22.253568891695114 70-71 21.98869054049321 27.364001122708153 28.247800828811176 22.399507507987458 72-73 21.780612171912466 27.473595887218146 28.326158557369354 22.419633383500038 74-75 21.817842575204427 27.54353083821805 28.126342649936543 22.51228393664098 76-77 21.87988348690831 27.416635425504627 28.06993745190505 22.63354363568201 78-79 21.858429986670306 27.541006168112986 28.099264973832046 22.501298871384666 80-81 21.863389363645872 27.434127057727864 28.150126576284162 22.552357002342106 82-83 21.758535721821477 27.418811631738805 28.256922504526173 22.565730141913548 84-85 21.767108597349797 27.44348106613866 28.05011836283895 22.739291973672593 86-87 21.850423292957647 27.486831481813308 27.999661607342922 22.663083617886123 88-89 21.520686669111704 27.48063804131291 28.063167796736 22.935507492839385 90-91 21.57550510515843 27.31100369899697 28.103991204785327 23.00949999105927 92-93 21.775631436608464 27.40100063017256 28.016904903137174 22.8064630300818 94-95 21.53665914816948 27.194128912835176 28.10480842630742 23.164403512687926 96-97 21.478004480729744 27.167362174151588 28.196361377130717 23.158271967987954 98-99 21.4460247809769 27.278781180624602 28.06709416075695 23.20809987764155 100 21.474284507860684 26.989415663949178 28.07054020710907 23.465759621081066 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 62.0 1 66.0 2 204.0 3 349.5 4 235.0 5 178.5 6 501.0 7 1178.5 8 2006.5 9 2364.5 10 1943.0 11 1517.5 12 1613.5 13 1935.5 14 2186.5 15 2468.5 16 2840.0 17 3204.0 18 3587.5 19 4066.0 20 4795.5 21 5868.0 22 7442.5 23 9747.0 24 13219.0 25 17902.5 26 23882.5 27 31670.0 28 41119.5 29 52160.5 30 63069.0 31 73652.0 32 84234.0 33 94198.5 34 104285.0 35 113100.0 36 120441.0 37 127458.0 38 133104.5 39 136410.5 40 139676.0 41 142617.0 42 143700.0 43 144782.5 44 143450.5 45 141530.0 46 141483.5 47 139868.5 48 138745.0 49 134972.5 50 129108.5 51 124880.5 52 116714.5 53 109410.0 54 105759.5 55 100083.5 56 91514.5 57 82492.5 58 75165.5 59 70557.0 60 64599.5 61 55830.0 62 48250.5 63 41611.5 64 33957.5 65 26311.5 66 20992.5 67 16415.5 68 12022.5 69 8653.5 70 5863.0 71 3998.5 72 3061.0 73 2178.0 74 1495.0 75 996.0 76 620.5 77 385.0 78 224.0 79 122.5 80 64.5 81 26.5 82 17.5 83 11.0 84 6.0 85 5.5 86 4.0 87 3.0 88 2.5 89 1.0 90 0.0 91 1.5 92 1.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.4529688379428E-4 2 4.6861719943509426E-4 3 1.7264844189714E-4 4 0.012159383122184288 5 5.919375150759086E-4 6 0.0035762891535836144 7 5.919375150759086E-4 8 0.13710752692945732 9 0.0011838750301518173 10-11 0.0 12-13 7.399218938448858E-5 14-15 2.219765681534657E-4 16-17 0.0 18-19 9.865625251265143E-5 20-21 0.0 22-23 2.7130469440979143E-4 24-25 4.932812625632572E-4 26-27 0.006153683750476633 28-29 0.008065148642909255 30-31 0.015045078508179344 32-33 0.01786911373635399 34-35 0.02648920379964691 36-37 0.03814297362770386 38-39 0.043310094853053975 40-41 0.05414995059788156 42-43 0.07081052524095556 44-45 0.10839855744827576 46-47 0.11588410060767318 48-49 0.11227081535939733 50-51 0.11429326853590667 52-53 0.10934812387871003 54-55 0.11516884277695646 56-57 0.0978176743662939 58-59 0.09162699452112501 60-61 0.09319316252976335 62-63 0.09195995937335522 64-65 3.699609469224429E-5 66-67 1.2332031564081428E-5 68-69 4.932812625632571E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 4.932812625632571E-5 78-79 1.2332031564081428E-5 80-81 2.466406312816286E-4 82-83 1.6031641033305858E-4 84-85 0.0 86-87 5.056132941273385E-4 88-89 2.7130469440979143E-4 90-91 1.1098828407673286E-4 92-93 9.125703357420258E-4 94-95 9.125703357420258E-4 96-97 2.096445365893843E-4 98-99 2.4664063128162856E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4054482.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.694198009626426 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5220427312891 38.403187244668 2 15.815077044679976 16.983557567490884 3 5.458670047335317 8.792967311725182 4 2.495371351644481 5.359478538509918 5 1.342805815442613 3.605044067142678 6 0.7887387149183096 2.5410415642009205 7 0.5151278414606603 1.9361563423762096 8 0.3500720824831386 1.5037471771593538 9 0.26413510525339023 1.2764270378492149 >10 1.3344721814311284 12.670650191023968 >50 0.07833848377721954 2.8760948474392523 >100 0.033492817448885825 2.944769349732708 >500 0.0013338251054143622 0.49656431909399823 >1k 2.2996980752070173E-4 0.25375231203473114 >5k 9.198792300827888E-5 0.3565621295530325 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7905 0.1949694190281274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6533 0.16113032441628794 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.4664063128162856E-5 0.0 4 0.0 0.0 0.0 4.932812625632571E-5 0.0 5 0.0 0.0 0.0 4.932812625632571E-5 0.0 6 0.0 0.0 0.0 4.932812625632571E-5 0.0 7 0.0 0.0 0.0 4.932812625632571E-5 0.0 8 0.0 0.0 0.0 4.932812625632571E-5 0.0 9 0.0 0.0 0.0 4.932812625632571E-5 0.0 10-11 0.0 0.0 0.0 6.166015782040715E-5 0.0 12-13 0.0 0.0 0.0 7.399218938448858E-5 0.0 14-15 0.0 0.0 0.0 9.865625251265143E-5 0.0 16-17 0.0 0.0 0.0 9.865625251265143E-5 0.0 18-19 0.0 0.0 0.0 9.865625251265143E-5 0.0 20-21 0.0 0.0 0.0 1.1098828407673286E-4 0.0 22-23 0.0 0.0 0.0 1.233203156408143E-4 0.0 24-25 0.0 0.0 0.0 1.4798437876897716E-4 0.0 26-27 0.0 0.0 0.0 2.219765681534657E-4 0.0 28-29 0.0 0.0 0.0 8.509101779216186E-4 0.0 30-31 0.0 0.0 0.0 0.004340875110556663 0.0 32-33 0.0 0.0 0.0 0.012208711248440616 0.0 34-35 0.0 0.0 0.0 0.02372682872929267 0.0 36-37 0.0 0.0 0.0 0.044987251145769055 0.0 38-39 0.0 0.0 0.0 0.08382081854106146 0.0 40-41 0.0 0.0 0.0 0.13020158925357173 0.0 42-43 0.0 0.0 0.0 0.17596575838787792 0.0 44-45 0.0 0.0 0.0 0.23196551372037166 0.0 46-47 0.0 0.0 0.0 0.3052177812110154 0.0 48-49 0.0 0.0 0.0 0.3849320332412377 0.0 50-51 0.0 0.0 0.0 0.4752148363218779 0.0 52-53 0.0 0.0 0.0 0.5646467292245965 0.0 54-55 0.0 0.0 0.0 0.6636112825263498 0.0 56-57 0.0 0.0 0.0 0.7836759418342466 0.0 58-59 0.0 0.0 0.0 0.9180087616617857 0.0 60-61 0.0 0.0 0.0 1.0662521130936087 0.0 62-63 0.0 0.0 0.0 1.2189473279200649 0.0 64-65 0.0 0.0 0.0 1.376316382709308 0.0 66-67 0.0 0.0 0.0 1.5554144771144625 0.0 68-69 0.0 0.0 0.0 1.760089204983522 0.0 70-71 0.0 0.0 0.0 1.9824973942417305 0.0 72-73 0.0 0.0 0.0 2.219716353408401 0.0 74-75 0.0 0.0 0.0 2.4555047969136377 0.0 76-77 0.0 0.0 0.0 2.705882033759183 0.0 78-79 0.0 0.0 0.0 2.9961040645882755 0.0 80-81 0.0 0.0 0.0 3.31697119385411 0.0 82-83 0.0 0.0 0.0 3.646211772551956 0.0 84-85 0.0 0.0 0.0 3.969335663594018 0.0 86-87 0.0 0.0 0.0 4.307479969081131 0.0 88 0.0 0.0 0.0 4.583865460495323 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8860 0.0 36.05661 1 GTATCAA 14445 0.0 34.27937 1 ATCAACG 16055 0.0 30.291704 3 TCAACGC 16075 0.0 30.22476 4 CAACGCA 16390 0.0 29.612614 5 AACGCAG 16860 0.0 28.842901 6 TATCAAC 17205 0.0 28.403664 2 GTACATG 13525 0.0 26.781002 1 TACATGG 13460 0.0 25.683594 2 ACGCAGA 19235 0.0 25.23175 7 ACATGGG 13370 0.0 24.449327 3 CGCAGAG 19775 0.0 24.400051 8 GCAGAGT 21220 0.0 22.469194 9 GAGTACT 12015 0.0 20.936686 12-13 AGAGTAC 17040 0.0 20.579134 10-11 GAGTACA 9780 0.0 20.27138 1 CATGGGG 8930 0.0 19.961872 4 GTACTTT 12770 0.0 19.60688 14-15 TACACCG 750 0.0 19.439112 5 CGACGCG 135 2.6175258E-8 19.13773 90-91 >>END_MODULE