FastQCFastQC Report
Wed 25 May 2016
SRR1032973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788574
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31900.034.1819651
GTATCAA49950.033.6858251
TCAACGC50050.031.646494
ATCAACG50950.031.179723
CAACGCA52150.030.4618555
AACGCAG53550.029.6650736
GTACATG109250.027.3181931
TATCAAC62100.026.6410142
TACATGG110650.025.7407322
ACGCAGA64700.024.116937
ACATGGG112450.023.6150383
CGCAGAG67900.022.8419088
CCGGTCG3351.8189894E-1221.044319
CGCGTAT1059.54431E-720.1424112-13
GAGTACA78950.019.8240221
CATGGGG75350.019.7731424
GCAGAGT82900.018.1419379
TAGACCG2102.144456E-517.90465
TATACCG3209.447831E-917.6248425
AGTACAT83250.017.1628362
Produced by FastQC (version 0.11.5)