FastQCFastQC Report
Wed 25 May 2016
SRR1032973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788574
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66140.17457755873318034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51980.13720201849033437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52400.050.7499471
GTATCAA93200.041.741191
TCAACGC108100.035.388154
ATCAACG109050.035.1230133
CAACGCA110600.034.5411225
AACGCAG114950.033.3972746
TATCAAC117350.032.638812
GTACATG112550.028.219651
ACGCAGA135500.028.1925987
CGCAGAG139200.027.4094288
TACATGG113750.027.3841692
ACATGGG118200.025.7958893
GCAGAGT156150.024.0968749
TACACCG5300.023.967545
GTGGTAT26150.022.0995711
CATGGGG76000.021.9170784
GAGTACT100800.021.01733412-13
CAGAGTA151750.020.44600910-11
AGAGTAC142650.019.95562410-11
GAGTACA80450.019.4477161
Produced by FastQC (version 0.11.5)