FastQCFastQC Report
Wed 25 May 2016
SRR1032972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893659
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.035.2072561
GTATCAA41550.034.7274441
TCAACGC41050.033.318494
ATCAACG41850.032.6815763
CAACGCA43100.031.7337325
GTACATG99800.031.0827051
AACGCAG44050.030.9421186
TACATGG101200.029.073632
TATCAAC48200.029.0585982
ACATGGG102950.026.9358753
ACGCAGA54300.024.4954017
CGCAGAG58400.022.3732938
CATGGGG70300.020.859544
CATGGGA33850.020.1332914
GAGTACA70450.019.5475391
GCAGAGT69650.018.3546359
TACACCG6000.018.0169815
ACCGTTC4203.6379788E-1217.9047668
AGTACAT71750.017.6212142
TCTACAC13500.017.4077113
Produced by FastQC (version 0.11.5)