##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032972_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2893659
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67034021631436	33.0	31.0	34.0	30.0	34.0
2	31.709333753562532	34.0	31.0	34.0	30.0	34.0
3	31.86446018691214	34.0	31.0	34.0	30.0	34.0
4	35.16947815896759	37.0	35.0	37.0	33.0	37.0
5	35.26074703342723	37.0	35.0	37.0	33.0	37.0
6	35.31971424414556	37.0	37.0	37.0	33.0	37.0
7	35.24677406702034	37.0	36.0	37.0	33.0	37.0
8	35.25451063860669	37.0	37.0	37.0	33.0	37.0
9	36.894964472316886	39.0	38.0	39.0	34.0	39.0
10-11	36.88554992139709	39.0	38.0	39.0	34.0	39.0
12-13	36.83095416564288	39.0	38.0	39.0	34.0	39.0
14-15	38.30629749393415	41.0	39.0	41.0	34.0	41.0
16-17	38.32752062354272	41.0	39.0	41.0	34.0	41.0
18-19	38.34597355804537	41.0	39.0	41.0	34.0	41.0
20-21	38.38324695480705	41.0	39.0	41.0	34.0	41.0
22-23	38.37316387314469	41.0	39.0	41.0	34.0	41.0
24-25	38.408069333670625	41.0	39.0	41.0	34.0	41.0
26-27	38.38556409030919	41.0	39.0	41.0	34.0	41.0
28-29	38.36218037439795	41.0	39.0	41.0	34.0	41.0
30-31	38.31464039128315	41.0	39.0	41.0	34.0	41.0
32-33	38.26195536516224	41.0	39.0	41.0	33.5	41.0
34-35	38.18914270824586	41.0	39.0	41.0	33.0	41.0
36-37	38.10437546372948	41.0	38.5	41.0	33.0	41.0
38-39	38.014553891802734	41.0	38.0	41.0	33.0	41.0
40-41	37.92891145777716	40.5	38.0	41.0	33.0	41.0
42-43	37.820147778297304	40.0	38.0	41.0	33.0	41.0
44-45	37.69848710577162	40.0	38.0	41.0	32.5	41.0
46-47	37.5759745706042	40.0	38.0	41.0	32.0	41.0
48-49	37.44269452620368	40.0	38.0	41.0	32.0	41.0
50-51	37.30482651895058	40.0	37.0	41.0	31.5	41.0
52-53	37.15035254672371	40.0	37.0	41.0	31.0	41.0
54-55	36.980815293025195	40.0	36.5	41.0	31.0	41.0
56-57	36.79716234704918	40.0	36.0	41.0	31.0	41.0
58-59	36.584727675237474	39.5	35.5	41.0	31.0	41.0
60-61	36.33948868888836	39.0	35.0	41.0	30.0	41.0
62-63	36.0851418567288	39.0	35.0	41.0	30.0	41.0
64-65	35.80180059226053	38.5	35.0	40.5	30.0	41.0
66-67	35.501552705415534	37.5	35.0	40.0	30.0	41.0
68-69	35.182754256807726	37.0	35.0	40.0	29.0	41.0
70-71	34.844000277848906	36.5	34.5	39.5	29.0	41.0
72-73	34.546663584064326	36.0	34.0	39.0	29.0	41.0
74-75	34.262576205420196	36.0	34.0	39.0	29.0	40.5
76-77	33.937293924405054	35.0	34.0	37.5	29.0	40.0
78-79	33.57496494922173	35.0	34.0	37.0	28.5	39.0
80-81	33.25900563957259	35.0	34.0	37.0	28.0	39.0
82-83	32.947853392538654	35.0	34.0	36.0	28.0	38.5
84-85	32.781027584798345	35.0	34.0	36.0	28.0	37.0
86-87	32.555127262749345	35.0	34.0	36.0	27.0	37.0
88-89	32.32609232808703	35.0	34.0	35.0	27.0	36.5
90-91	32.13433822022567	35.0	34.0	35.0	27.0	36.0
92-93	31.947344866827777	35.0	33.5	35.0	26.5	36.0
94-95	31.76990153297261	35.0	33.0	35.0	26.0	36.0
96-97	31.601058210383464	35.0	33.0	35.0	25.0	36.0
98-99	31.425143391118304	35.0	33.0	35.0	25.0	35.0
100	31.304930885083557	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.45876701818846755
1101	2	0.517205616245004
1101	3	0.46696117268421844
1101	4	0.3791982999309269
1101	5	0.3657051305153445
1101	6	0.3834844766853607
1101	7	0.39216219820119136
1101	8	0.33991896741025585
1101	9	0.44439251120947887
1101	10-11	0.43471609267602673
1101	12-13	0.3120931529555051
1101	14-15	0.4143203629423837
1101	16-17	0.40108031703819336
1101	18-19	0.42206118108584434
1101	20-21	0.39647069786762756
1101	22-23	0.40745300479073876
1101	24-25	0.36802629156507294
1101	26-27	0.4001120286687794
1101	28-29	0.45514974696600774
1101	30-31	0.46441665824128364
1101	32-33	0.4614116770860477
1101	34-35	0.47476795622878853
1101	36-37	0.4697711225408554
1101	38-39	0.465933870628902
1101	40-41	0.4334906711602713
1101	42-43	0.5064202029576492
1101	44-45	0.5166019156769934
1101	46-47	0.47081762007260153
1101	48-49	0.527885254926467
1101	50-51	0.5353400496615706
1101	52-53	0.45009803888068944
1101	54-55	0.47705919249615647
1101	56-57	0.5090535594486809
1101	58-59	0.4902622349623087
1101	60-61	0.5153345621301497
1101	62-63	0.5692354357201808
1101	64-65	0.5004858906207801
1101	66-67	0.5262010421597338
1101	68-69	0.5137926863613771
1101	70-71	0.4510821646372989
1101	72-73	0.4352637238862016
1101	74-75	0.4970314503935569
1101	76-77	0.4654014181718935
1101	78-79	0.4757397984987861
1101	80-81	0.4819180400376766
1101	82-83	0.43098051342333576
1101	84-85	0.4141624572589748
1101	86-87	0.4417320784067442
1101	88-89	0.38556323477810395
1101	90-91	0.36148371006412106
1101	92-93	0.3527913348405072
1101	94-95	0.29950877675609533
1101	96-97	0.321911116210547
1101	98-99	0.3376272335682309
1101	100	0.3767883542667434
1102	1	0.27524752804373165
1102	2	0.36194887656307273
1102	3	0.36711828228547105
1102	4	0.35162053688172534
1102	5	0.32580647469703194
1102	6	0.2889626129188443
1102	7	0.29060155004246724
1102	8	0.28591355411601427
1102	9	0.3589227985581047
1102	10-11	0.3228809924582521
1102	12-13	0.31293782928426594
1102	14-15	0.40160744710964025
1102	16-17	0.3924447740926169
1102	18-19	0.3848262382388725
1102	20-21	0.346019285285152
1102	22-23	0.3339064155252558
1102	24-25	0.3532734649308793
1102	26-27	0.37641290073807454
1102	28-29	0.38971790778460047
1102	30-31	0.41941092824647086
1102	32-33	0.44789159924953026
1102	34-35	0.4521387538244781
1102	36-37	0.4438187843438328
1102	38-39	0.41250952125267304
1102	40-41	0.3981056840731654
1102	42-43	0.4232191764560227
1102	44-45	0.36837155356091245
1102	46-47	0.38446277830860964
1102	48-49	0.44245651745165304
1102	50-51	0.4653779884306246
1102	52-53	0.3821187844003546
1102	54-55	0.38874501900302505
1102	56-57	0.4097853336575028
1102	58-59	0.45066046143913496
1102	60-61	0.4716258678833327
1102	62-63	0.4186000429625025
1102	64-65	0.3854926001018839
1102	66-67	0.35892358960768433
1102	68-69	0.41302989508663757
1102	70-71	0.3574905516519209
1102	72-73	0.2999093947200322
1102	74-75	0.3467844892497709
1102	76-77	0.34248503038674016
1102	78-79	0.317555864032947
1102	80-81	0.3243693024322667
1102	82-83	0.3864032107326807
1102	84-85	0.3014720696109521
1102	86-87	0.3336697308393042
1102	88-89	0.3459425898190247
1102	90-91	0.31444169336713657
1102	92-93	0.3575424963479996
1102	94-95	0.32729406030105324
1102	96-97	0.3409397923042228
1102	98-99	0.2654222140274811
1102	100	0.2837944891158841
1103	1	0.15320583776096797
1103	2	0.2856688841202235
1103	3	0.2662958123566348
1103	4	0.1911035018039442
1103	5	0.16614213815191192
1103	6	0.1557407475620849
1103	7	0.18734882631930816
1103	8	0.20465025038152618
1103	9	0.24461207370137572
1103	10-11	0.2425670558123727
1103	12-13	0.2855192260976622
1103	14-15	0.35457835207846955
1103	16-17	0.35358905269958285
1103	18-19	0.366272802884005
1103	20-21	0.29743341715743554
1103	22-23	0.3366638309796315
1103	24-25	0.28553588699776355
1103	26-27	0.31348198569304486
1103	28-29	0.34250177326730835
1103	30-31	0.31588709173284
1103	32-33	0.38777194435942874
1103	34-35	0.31875315019704686
1103	36-37	0.2816631070341771
1103	38-39	0.2721502417010626
1103	40-41	0.30827697949479216
1103	42-43	0.34604366162511013
1103	44-45	0.28581093891263976
1103	46-47	0.23276462351297056
1103	48-49	0.294579447107175
1103	50-51	0.2736244145279798
1103	52-53	0.27468884548732575
1103	54-55	0.36780320906536446
1103	56-57	0.37181285428221855
1103	58-59	0.38702137837344
1103	60-61	0.42512514365635923
1103	62-63	0.4082990164494973
1103	64-65	0.31890591328281204
1103	66-67	0.30124646888219786
1103	68-69	0.29144257850499145
1103	70-71	0.2799626846577752
1103	72-73	0.3470118570917222
1103	74-75	0.23880035926677579
1103	76-77	0.2546441713905807
1103	78-79	0.29230868222049367
1103	80-81	0.25260943090382426
1103	82-83	0.2411931849124187
1103	84-85	0.24249795285297182
1103	86-87	0.24916063077002804
1103	88-89	0.28710953987885546
1103	90-91	0.211972079411602
1103	92-93	0.2897391082253229
1103	94-95	0.2451113032004777
1103	96-97	0.26735801763327416
1103	98-99	0.304908957525182
1103	100	0.313728364045037
1104	1	0.02388463106584382
1104	2	0.0648663496091686
1104	3	0.08779875848917484
1104	4	0.035704381822291964
1104	5	0.14671968203454355
1104	6	0.10312871627739639
1104	7	0.18519008818845606
1104	8	0.12019978871764181
1104	9	0.14455384254552683
1104	10-11	0.12564280726724064
1104	12-13	0.24654238390046146
1104	14-15	0.2106066415018688
1104	16-17	0.22717776604579853
1104	18-19	0.21925405609126614
1104	20-21	0.20812402046860257
1104	22-23	0.21442617143536324
1104	24-25	0.21345890904917297
1104	26-27	0.1934668455925248
1104	28-29	0.15348062079994662
1104	30-31	0.16353591782912957
1104	32-33	0.22402866368889818
1104	34-35	0.16505292418737127
1104	36-37	0.2252980777548217
1104	38-39	0.2503403991799402
1104	40-41	0.3201566812056953
1104	42-43	0.2893456229232285
1104	44-45	0.2272180220048554
1104	46-47	0.17108863986135958
1104	48-49	0.19386265797310642
1104	50-51	0.24933920196605897
1104	52-53	0.20486368453277493
1104	54-55	0.21298267020880246
1104	56-57	0.23399526701737727
1104	58-59	0.161604843636745
1104	60-61	0.2622557852545171
1104	62-63	0.2629797095458244
1104	64-65	0.2654628142206974
1104	66-67	0.29527477286339376
1104	68-69	0.2586914657439934
1104	70-71	0.2608400280068679
1104	72-73	0.2922514601008146
1104	74-75	0.23091021962118674
1104	76-77	0.25795979881308995
1104	78-79	0.29824334686362874
1104	80-81	0.3134689045669674
1104	82-83	0.2736366733953872
1104	84-85	0.3211398567206061
1104	86-87	0.25674523881976086
1104	88-89	0.2231142274869029
1104	90-91	0.23234288449372542
1104	92-93	0.11227474751243349
1104	94-95	0.17605715914387687
1104	96-97	0.20479491871673972
1104	98-99	0.160446922419073
1104	100	0.1915746847458486
1105	1	0.023137275685812142
1105	2	0.024623685285511954
1105	3	0.0737371283163668
1105	4	0.053073977487834156
1105	5	-0.009734614502093564
1105	6	0.05373420017158992
1105	7	-0.003996371223777828
1105	8	0.07404624891141509
1105	9	0.04264492102952033
1105	10-11	0.04706454124337256
1105	12-13	0.06605007433464039
1105	14-15	0.11521765614816104
1105	16-17	0.07721701860523922
1105	18-19	0.11242885293818716
1105	20-21	0.08283961134580409
1105	22-23	0.09379495022409401
1105	24-25	0.13429759533124752
1105	26-27	0.10852823192237082
1105	28-29	0.1400680779252852
1105	30-31	0.10501869944832976
1105	32-33	0.14651275764020255
1105	34-35	0.17515940074745373
1105	36-37	0.20082849087729215
1105	38-39	0.1416335133297011
1105	40-41	0.1674102134900295
1105	42-43	0.1645675749825628
1105	44-45	0.11152377196239627
1105	46-47	0.16148921509365266
1105	48-49	0.1732188791656668
1105	50-51	0.183460970478464
1105	52-53	0.14211164416798994
1105	54-55	0.1608566755070555
1105	56-57	0.18066982620805305
1105	58-59	0.22661057541396445
1105	60-61	0.27133676316091737
1105	62-63	0.18662423012622043
1105	64-65	0.2498466779513251
1105	66-67	0.27993272253252144
1105	68-69	0.23778747075368045
1105	70-71	0.20944610893361215
1105	72-73	0.1823244961621171
1105	74-75	0.1680390489902166
1105	76-77	0.16661931455604417
1105	78-79	0.19636774729164586
1105	80-81	0.19321608021568437
1105	82-83	0.15333697387414702
1105	84-85	0.16527631969236722
1105	86-87	0.15210754359790712
1105	88-89	0.22653349632978603
1105	90-91	0.22685515165952097
1105	92-93	0.24245270568527033
1105	94-95	0.17035844488173524
1105	96-97	0.22327993624413622
1105	98-99	0.14820968306904447
1105	100	0.16082307693327635
1106	1	0.03777394221201291
1106	2	0.04086868035613378
1106	3	0.07982729587241977
1106	4	0.0987251913594207
1106	5	0.0916438956749559
1106	6	0.14031745769611348
1106	7	0.06973149158596215
1106	8	0.1193567272842131
1106	9	0.05114511548109846
1106	10-11	0.07781567341625362
1106	12-13	0.09322893619588513
1106	14-15	0.1048833917743508
1106	16-17	0.056521857909658024
1106	18-19	0.024312532261255626
1106	20-21	0.05244894204056294
1106	22-23	0.04269037922286145
1106	24-25	0.10202994907650975
1106	26-27	0.207151321854127
1106	28-29	0.08902712768860965
1106	30-31	0.08849713762595712
1106	32-33	0.07022195342715776
1106	34-35	0.08314139009807064
1106	36-37	0.12180445970892606
1106	38-39	0.13518285550688347
1106	40-41	0.15580986302235544
1106	42-43	0.13806654003547436
1106	44-45	0.09587150212859541
1106	46-47	0.1531961032168354
1106	48-49	0.17528082759734076
1106	50-51	0.10948484421146532
1106	52-53	0.11307336819535152
1106	54-55	0.1060078498964998
1106	56-57	0.13432404455792124
1106	58-59	0.07716882433380334
1106	60-61	0.1068573867825009
1106	62-63	0.12807493370556955
1106	64-65	0.08147350254800045
1106	66-67	0.12835792229542164
1106	68-69	0.16743361819855807
1106	70-71	0.20647445480633309
1106	72-73	0.22425225135906857
1106	74-75	0.15884842402771682
1106	76-77	0.06566513460330725
1106	78-79	0.06588005221057358
1106	80-81	0.05883159960524864
1106	82-83	0.035986226900064366
1106	84-85	0.038263956824295065
1106	86-87	0.008964467402975629
1106	88-89	0.006043544108919718
1106	90-91	-0.030353297419125624
1106	92-93	0.023503707564362486
1106	94-95	0.0022857282657717803
1106	96-97	-0.001368901520667265
1106	98-99	0.01376380102303898
1106	100	-0.044205440348367375
1107	1	0.023710457519321437
1107	2	0.1119873817627024
1107	3	0.07518018723752462
1107	4	0.09906179506051416
1107	5	0.12172127267308497
1107	6	0.06868537548152176
1107	7	0.03543814538419099
1107	8	0.043548593277229486
1107	9	0.016843974136108386
1107	10-11	0.03467630452151127
1107	12-13	0.08710985340277944
1107	14-15	0.17720199591427388
1107	16-17	0.14799231803132784
1107	18-19	0.15114508410969307
1107	20-21	0.1999663249483561
1107	22-23	0.18081424833852822
1107	24-25	0.17289018132154865
1107	26-27	0.17840108106791064
1107	28-29	0.1491632047309963
1107	30-31	0.15057064781452567
1107	32-33	0.12730209890440847
1107	34-35	0.17890260571351035
1107	36-37	0.16646566028863674
1107	38-39	0.13968643410542114
1107	40-41	0.14245848297333907
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2216	48-49	-0.31175868633700077
2216	50-51	-0.2919790904826911
2216	52-53	-0.25677949985084325
2216	54-55	-0.31429796516607666
2216	56-57	-0.2872969193122472
2216	58-59	-0.29357422368223496
2216	60-61	-0.23130254749579393
2216	62-63	-0.21540893700419161
2216	64-65	-0.228008288154129
2216	66-67	-0.3068800385586101
2216	68-69	-0.2812112374281739
2216	70-71	-0.28893235097388725
2216	72-73	-0.27691449867069196
2216	74-75	-0.29955659177865357
2216	76-77	-0.261355575204405
2216	78-79	-0.2161834850122304
2216	80-81	-0.1992286214142851
2216	82-83	-0.213419880212669
2216	84-85	-0.23804929259804908
2216	86-87	-0.25736226956887975
2216	88-89	-0.20990037208569134
2216	90-91	-0.20981678681060245
2216	92-93	-0.22980762494648843
2216	94-95	-0.22802977047110673
2216	96-97	-0.15839144137435923
2216	98-99	-0.16891020244630894
2216	100	-0.18152001844704557
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37722.0
3	6135.0
4	4064.0
5	3433.0
6	2784.0
7	2992.0
8	3555.0
9	3885.0
10	3956.0
11	4291.0
12	4548.0
13	5057.0
14	5458.0
15	5746.0
16	5694.0
17	5624.0
18	5546.0
19	5346.0
20	5446.0
21	5817.0
22	6772.0
23	9937.0
24	15047.0
25	20848.0
26	22174.0
27	19296.0
28	19940.0
29	24936.0
30	30925.0
31	36749.0
32	47737.0
33	63660.0
34	93621.0
35	146482.0
36	279838.0
37	589209.0
38	936115.0
39	402604.0
40	670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91012693950729	19.227060691914385	13.028833916200005	23.833978452378318
2	15.504614602445557	19.87251695998257	40.27914151274571	24.343726924826164
3	18.34043328803704	25.610989809890455	29.467031296637224	26.58154560543528
4	12.458699102577715	16.52924776249697	38.43521262720573	32.57684050771959
5	13.12171559367125	38.354464440735256	35.18628554352796	13.33753442206554
6	31.212718294792257	37.74707911339103	17.773475261047594	13.266727330769115
7	28.032678194626932	32.51176700686566	22.00202881217647	17.453525986330938
8	25.605278075312043	36.580366934972055	19.874681680608795	17.939673309107107
9	25.505135135602515	17.196158894359222	20.504123499189827	36.79458247084844
10-11	24.23771796563391	27.071446296605327	28.35084090832452	20.339994829436243
12-13	24.815366003134816	24.47037693902876	28.38232812108753	22.331928936748895
14-15	22.108633910624338	26.254100038873418	25.365631471697803	26.27163457880444
16-17	22.03114913781938	29.572953472041014	26.007508620595743	22.388388769543866
18-19	22.009852008902673	28.6308986210058	27.800531317487874	21.55871805260365
20-21	23.031534017073856	27.919106939953064	27.311065394684032	21.73829364828904
22-23	22.821602594408546	27.631077013635725	27.28438232872169	22.26293806323404
24-25	22.48037189600317	27.759187550482828	27.350275562100514	22.410164991413488
26-27	22.279240558643664	27.79555710033054	27.416787496314587	22.508414844711204
28-29	22.404463995462688	27.753529212402906	27.477434482186208	22.364572309948194
30-31	22.502367770173514	28.023573774046678	27.200383477607215	22.273674978172593
32-33	22.326332599280818	27.827022989072216	27.516593996332332	22.330050415314634
34-35	22.462799530911138	27.59213615612041	27.525321080675052	22.4197432322934
36-37	22.6313751847344	27.92518393035952	27.08839567976985	22.355045205136236
38-39	22.44608979037661	28.11957640613358	27.227697006197797	22.20663679729201
40-41	22.29792261222141	27.750615939873757	27.51487455023593	22.436586897668903
42-43	22.287489019084315	27.783688065906247	27.438316651564303	22.490506263445138
44-45	22.333081375472034	27.88338276514847	27.42155801264908	22.36197784673042
46-47	22.35547395356953	27.897814437567998	27.379616462595408	22.36709514626706
48-49	22.26592588723878	27.863242423791736	27.417024622049958	22.453807066919527
50-51	22.32004519979586	27.844499282036455	27.365885741155765	22.46956977701192
52-53	22.339518511434342	27.734846129182106	27.429190162146043	22.49644519723751
54-55	22.317899027967	28.063315136466127	27.284924339978772	22.3338614955881
56-57	22.242317555729528	27.854415015675915	27.384215340348717	22.51905208824584
58-59	22.221482440955366	27.431971347983307	27.664639281693525	22.6819069293678
60-61	22.386682696331693	27.868236706872256	27.312691911544473	22.432388685251578
62-63	22.194596991365064	27.880854072143872	27.319112862893157	22.605436073597904
64-65	22.243954065684928	27.64937255786968	27.482458165480782	22.624215210964607
66-67	22.104357907238875	27.69751452537752	27.406090438308606	22.792037129075
68-69	22.398378742840897	27.837939305923054	27.305555752504073	22.45812619873197
70-71	22.304916156098205	27.63302016161805	27.43823461766593	22.62382906461782
72-73	22.141340198323604	27.703656257527747	27.52756420753995	22.627439336608695
74-75	22.231174051753104	27.794842743216396	27.390384905036534	22.583598299993966
76-77	22.255366368767078	27.679622108310113	27.344802822013687	22.720208700909122
78-79	22.24560575887512	27.771029792508678	27.457703851216607	22.525660597399597
80-81	22.241238641594737	27.650144689646154	27.487920048724458	22.620696620034646
82-83	22.160926990040565	27.664355370853254	27.549479409396106	22.625238229710074
84-85	22.164528260645515	27.66832122503723	27.412079106151126	22.75507140816613
86-87	22.30610363516289	27.60367139122154	27.495989376711876	22.594235596903697
88-89	22.102023679337748	27.576046199401965	27.543346523874046	22.77858359738624
90-91	22.152205718716374	27.537639538508973	27.560482033711786	22.749672709062867
92-93	22.278155401670144	27.603283434412496	27.45528299658435	22.663278167333008
94-95	22.074288544220096	27.40507121474538	27.62654519950642	22.8940950415281
96-97	22.22484864131229	27.37668976781898	27.54554334626119	22.852918244607537
98-99	22.12805772363881	27.504443086474446	27.5587388308985	22.808760358988245
100	22.224823635154486	27.28421827291619	27.541931068397986	22.949027023531336
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2859.0
1	1839.5
2	746.5
3	604.0
4	482.0
5	399.5
6	349.5
7	335.0
8	348.5
9	345.5
10	318.5
11	331.0
12	350.5
13	355.0
14	388.0
15	476.5
16	558.5
17	696.5
18	933.0
19	1224.0
20	1701.5
21	2379.5
22	3392.5
23	5028.5
24	7262.0
25	10208.5
26	14261.0
27	19520.5
28	26027.5
29	33304.5
30	40787.0
31	48034.5
32	54639.0
33	62084.5
34	70154.5
35	76406.0
36	81981.0
37	88076.5
38	93894.5
39	96977.0
40	99368.5
41	102673.5
42	104154.5
43	105679.5
44	105278.5
45	103769.5
46	103810.0
47	101816.0
48	99494.5
49	97820.0
50	95145.0
51	92340.5
52	86980.5
53	82117.0
54	79101.0
55	74938.0
56	69381.5
57	62754.5
58	57373.5
59	53342.0
60	47822.0
61	41095.5
62	35800.0
63	30733.5
64	25278.5
65	20594.5
66	16380.0
67	12477.0
68	9589.0
69	7396.5
70	5504.0
71	4094.0
72	3151.5
73	2286.0
74	1667.5
75	1224.0
76	806.5
77	553.0
78	365.0
79	221.5
80	163.0
81	114.0
82	75.5
83	57.5
84	47.5
85	36.0
86	35.5
87	28.5
88	19.5
89	15.5
90	13.0
91	11.5
92	7.5
93	5.0
94	4.5
95	4.0
96	3.0
97	3.0
98	2.5
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07613198376173558
2	0.07927679108008234
3	0.08038265738983066
4	0.08217969014317168
5	0.08103926551124373
6	0.08045177403418993
7	0.07989884087931577
8	0.07955325765751942
9	0.07962237430187868
10-11	0.07919039527463326
12-13	0.07820548309251367
14-15	0.07682315020532827
16-17	0.07542353815705306
18-19	0.07490516332435854
20-21	0.07473237171346035
22-23	0.07637389201699302
24-25	0.07621837956718466
26-27	0.07573456305666977
28-29	0.07184675181146084
30-31	0.07690954601077736
32-33	0.07521618822397524
34-35	0.07243424328851465
36-37	0.06937583177561696
38-39	0.07148388942857468
40-41	0.07405848443095749
42-43	0.07875841624738783
44-45	0.07957053681860925
46-47	0.08257711084823748
48-49	0.08535905578369808
50-51	0.08624029299927877
52-53	0.08639580544908713
54-55	0.0864649220934464
56-57	0.08433958527939885
58-59	0.08342378974163853
60-61	0.0813157320886808
62-63	0.08228336510971057
64-65	0.08143668621630952
66-67	0.08086647390034556
68-69	0.08026170326220194
70-71	0.07858562463648965
72-73	0.07856834547539983
74-75	0.07801541232052568
76-77	0.07770438742090896
78-79	0.0781363664481544
80-81	0.0794150243688009
82-83	0.07877569540847763
84-85	0.07785989987071731
86-87	0.07863746211975911
88-89	0.07578640053993922
90-91	0.07321180553755643
92-93	0.07303901392665825
94-95	0.07336731798736479
96-97	0.072589755738323
98-99	0.07215777671107756
100	0.07146661026748488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2893659.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.69273425453298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26211439483907	47.66511091304134
2	13.570335038921025	16.74382146602264
3	4.053325497784339	7.501821983457953
4	1.7334891776413348	4.277747486773431
5	0.9074756053369654	2.799232568126243
6	0.5554330816356738	2.0559711300915566
7	0.35082954320991094	1.515054364452176
8	0.2551228398516285	1.259138044898263
9	0.20258361269263966	1.1248143283953227
>10	1.039136623027321	11.251906280666745
>50	0.053728106179962286	2.2168573544238597
>100	0.01631413656541297	1.4784298375994616
>500	0.0	0.0
>1k	1.1234231471518699E-4	0.11009424205097498
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.455832217963485E-5	0.0
5	0.0	0.0	0.0	3.455832217963485E-5	0.0
6	0.0	0.0	0.0	3.455832217963485E-5	0.0
7	0.0	0.0	0.0	3.455832217963485E-5	0.0
8	0.0	0.0	0.0	3.455832217963485E-5	0.0
9	0.0	0.0	0.0	6.91166443592697E-5	0.0
10-11	0.0	0.0	0.0	6.91166443592697E-5	0.0
12-13	0.0	1.7279161089817424E-5	0.0	1.0367496653890455E-4	0.0
14-15	0.0	1.0367496653890455E-4	0.0	1.382332887185394E-4	0.0
16-17	0.0	1.7279161089817424E-4	0.0	2.073499330778091E-4	0.0
18-19	0.0	1.7279161089817424E-4	0.0	2.4190825525744394E-4	0.0
20-21	0.0	1.7279161089817424E-4	0.0	2.591874163472614E-4	0.0
22-23	0.0	1.7279161089817424E-4	0.0	3.283040607065311E-4	0.0
24-25	0.0	1.9007077198799168E-4	0.0	4.838165105148879E-4	0.0
26-27	0.0	2.073499330778091E-4	0.0	6.91166443592697E-4	0.0
28-29	0.0	2.073499330778091E-4	0.0	0.0018488702366104646	0.0
30-31	0.0	2.073499330778091E-4	0.0	0.0059094730927175595	0.0
32-33	0.0	2.4190825525744394E-4	0.0	0.013598699777686313	0.0
34-35	0.0	2.764665774370788E-4	0.0	0.02522757519113344	0.0
36-37	0.0	3.283040607065311E-4	0.0	0.04762136796353682	0.0
38-39	0.0	3.8014154397598336E-4	0.0	0.08701785524832055	0.0
40-41	0.0	3.8014154397598336E-4	0.0	0.13653993093173727	0.0
42-43	0.0	3.8014154397598336E-4	0.0	0.1871333146027227	0.0
44-45	0.0	3.8014154397598336E-4	0.0	0.24672914120150302	0.0
46-47	0.0	3.8014154397598336E-4	0.0	0.3195435260339936	0.0
48-49	0.0	3.8014154397598336E-4	0.0	0.4037103197024943	0.0
50-51	0.0	3.8014154397598336E-4	0.0	0.49815821421943635	0.0
52-53	0.0	3.8014154397598336E-4	0.0	0.5940575582679231	0.0
54-55	0.0	3.8014154397598336E-4	0.0	0.6983200162838814	0.0
56-57	0.0	3.8014154397598336E-4	0.0	0.8221252054924233	0.0
58-59	0.0	3.8014154397598336E-4	0.0	0.9609114273658368	0.0
60-61	0.0	3.8014154397598336E-4	0.0	1.1112919663305179	0.0
62-63	0.0	3.8014154397598336E-4	0.0	1.2644371710695697	0.0
64-65	0.0	3.8014154397598336E-4	0.0	1.42435580695583	0.0
66-67	0.0	3.8014154397598336E-4	0.0	1.605873394204362	0.0
68-69	0.0	3.8014154397598336E-4	0.0	1.8155560140292963	0.0
70-71	0.0	3.8014154397598336E-4	0.0	2.051088258844598	0.0
72-73	0.0	4.4925818833525306E-4	0.0	2.2930310724242213	0.0
74-75	0.0	4.4925818833525306E-4	0.0	2.530619537409211	0.0
76-77	0.0	4.4925818833525306E-4	0.0	2.787025008821012	1.7279161089817424E-5
78-79	0.0	4.4925818833525306E-4	0.0	3.082222196879453	3.455832217963485E-5
80-81	0.0	4.4925818833525306E-4	0.0	3.401852118718895	3.455832217963485E-5
82-83	0.0	4.4925818833525306E-4	0.0	3.7399189054411734	3.455832217963485E-5
84-85	0.0	4.4925818833525306E-4	0.0	4.066356816750004	3.455832217963485E-5
86-87	0.0	4.6653734942507047E-4	0.0	4.407395619179731	3.455832217963485E-5
88	0.0	4.838165105148879E-4	0.0	4.688907711655036	3.455832217963485E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2710	0.0	35.207256	1
GTATCAA	4155	0.0	34.727444	1
TCAACGC	4105	0.0	33.31849	4
ATCAACG	4185	0.0	32.681576	3
CAACGCA	4310	0.0	31.733732	5
GTACATG	9980	0.0	31.082705	1
AACGCAG	4405	0.0	30.942118	6
TACATGG	10120	0.0	29.07363	2
TATCAAC	4820	0.0	29.058598	2
ACATGGG	10295	0.0	26.935875	3
ACGCAGA	5430	0.0	24.495401	7
CGCAGAG	5840	0.0	22.373293	8
CATGGGG	7030	0.0	20.85954	4
CATGGGA	3385	0.0	20.133291	4
GAGTACA	7045	0.0	19.547539	1
GCAGAGT	6965	0.0	18.354635	9
TACACCG	600	0.0	18.016981	5
ACCGTTC	420	3.6379788E-12	17.904766	8
AGTACAT	7175	0.0	17.621214	2
TCTACAC	1350	0.0	17.407711	3
>>END_MODULE