FastQCFastQC Report
Wed 25 May 2016
SRR1032972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893659
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71480.2470228869400299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56250.19439056226044604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.1131439468161245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44600.056.250491
GTATCAA85900.043.4804341
TCAACGC104350.035.169654
ATCAACG105250.034.7801063
CAACGCA106400.034.4436455
TATCAAC108600.033.8805432
AACGCAG109800.033.4621966
ACGCAGA127950.028.5306847
CGCAGAG131250.027.7053368
GTACATG103600.027.0275341
TACATGG105150.025.2820662
GCAGAGT145250.024.8032789
ACATGGG109350.023.7086163
GAGTACT89800.021.68093712-13
GTGGTAT21150.021.3245941
CAGAGTA139300.021.20944810-11
AGAGTAC133100.020.80346310-11
CATGGGG72250.020.3832024
GAGTACA78600.019.6051961
TGGTATC21600.019.3869572
Produced by FastQC (version 0.11.5)