##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032972_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2893659
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81527989303508	34.0	31.0	34.0	31.0	34.0
2	32.8724552547484	34.0	31.0	34.0	31.0	34.0
3	32.958630923685206	34.0	31.0	34.0	31.0	34.0
4	36.37381253285201	37.0	37.0	37.0	35.0	37.0
5	36.34900415010891	37.0	37.0	37.0	35.0	37.0
6	36.406413471663384	37.0	37.0	37.0	35.0	37.0
7	36.377263181321645	37.0	37.0	37.0	35.0	37.0
8	36.311731617305284	37.0	37.0	37.0	35.0	37.0
9	38.1500605289013	39.0	39.0	39.0	37.0	39.0
10-11	38.16882414272034	39.0	39.0	39.0	37.0	39.0
12-13	38.15624163040634	39.0	39.0	39.0	37.0	39.0
14-15	39.70998258606146	41.0	40.0	41.0	37.0	41.0
16-17	39.7200466606466	41.0	40.0	41.0	37.0	41.0
18-19	39.74461866446599	41.0	40.0	41.0	37.0	41.0
20-21	39.751106298288775	41.0	40.0	41.0	37.0	41.0
22-23	39.71608783204932	41.0	40.0	41.0	37.0	41.0
24-25	39.69573038841135	41.0	40.0	41.0	37.0	41.0
26-27	39.62091352160016	41.0	40.0	41.0	37.0	41.0
28-29	39.55992896882459	41.0	40.0	41.0	37.0	41.0
30-31	39.45022703089756	41.0	39.5	41.0	36.0	41.0
32-33	39.32672647329903	41.0	39.0	41.0	35.5	41.0
34-35	39.23542390447527	41.0	39.0	41.0	35.0	41.0
36-37	39.14859853908149	41.0	39.0	41.0	35.0	41.0
38-39	39.05444715496885	41.0	39.0	41.0	35.0	41.0
40-41	38.95374074830518	41.0	39.0	41.0	35.0	41.0
42-43	38.814531359776666	41.0	39.0	41.0	35.0	41.0
44-45	38.63704430964395	41.0	39.0	41.0	35.0	41.0
46-47	38.41171074407869	41.0	38.0	41.0	35.0	41.0
48-49	38.20233949473659	41.0	38.0	41.0	34.0	41.0
50-51	38.03861235895452	40.0	38.0	41.0	34.0	41.0
52-53	37.891590024947654	40.0	38.0	41.0	34.0	41.0
54-55	37.737831409989916	40.0	37.0	41.0	34.0	41.0
56-57	37.52186971581655	40.0	37.0	41.0	33.0	41.0
58-59	37.34587472124393	40.0	36.0	41.0	33.0	41.0
60-61	37.14641721778551	39.5	36.0	41.0	33.0	41.0
62-63	36.90131819264122	39.0	35.0	41.0	33.0	41.0
64-65	36.605006325900874	39.0	35.0	41.0	32.0	41.0
66-67	36.321307555589655	38.5	35.0	41.0	32.0	41.0
68-69	36.019103149334455	37.0	35.0	40.0	32.0	41.0
70-71	35.69418926003375	37.0	35.0	40.0	31.0	41.0
72-73	35.34871524253549	36.5	35.0	39.0	31.0	41.0
74-75	35.00384910592437	36.0	35.0	39.0	31.0	41.0
76-77	34.66178081107691	35.5	35.0	38.5	31.0	40.0
78-79	34.32501168935248	35.0	35.0	37.0	30.5	39.0
80-81	34.003016595943066	35.0	34.0	37.0	30.0	39.0
82-83	33.70984331602307	35.0	34.0	36.5	30.0	39.0
84-85	33.44370051896232	35.0	34.0	36.0	30.0	37.0
86-87	33.30714762865977	35.0	34.0	36.0	30.0	37.0
88-89	33.128162820843784	35.0	34.0	35.0	30.0	37.0
90-91	32.963285238516356	35.0	34.0	35.0	30.0	36.0
92-93	32.81278357263244	35.0	34.0	35.0	30.0	36.0
94-95	32.72152852150167	35.0	34.0	35.0	29.5	36.0
96-97	32.66352704309665	35.0	34.0	35.0	30.0	36.0
98-99	32.55601178300553	35.0	34.0	35.0	30.0	35.5
100	32.47675348062781	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19331149296179007
1101	2	0.22122987353131407
1101	3	0.21127342913068503
1101	4	0.10081897708308674
1101	5	0.10802336123469303
1101	6	0.13532622059465638
1101	7	0.11982848728493423
1101	8	0.010150125811819066
1101	9	0.11914371102187715
1101	10-11	0.1775486522632832
1101	12-13	0.19336264628586264
1101	14-15	0.22194019220160754
1101	16-17	0.24094868794848168
1101	18-19	0.21872264458070134
1101	20-21	0.254197888456261
1101	22-23	0.2257723238756526
1101	24-25	0.26335305224834826
1101	26-27	0.27851893731348554
1101	28-29	0.2501536853977768
1101	30-31	0.2739454492193474
1101	32-33	0.23364587697703598
1101	34-35	0.23036241967481175
1101	36-37	0.21864288374604968
1101	38-39	0.23670217262411342
1101	40-41	0.24194866158945416
1101	42-43	0.23588879084653058
1101	44-45	0.24358457170865933
1101	46-47	0.29248117309685995
1101	48-49	0.23525231872469732
1101	50-51	0.2124785173896413
1101	52-53	0.250825391532878
1101	54-55	0.3252828782298991
1101	56-57	0.37005196436507504
1101	58-59	0.2765061733881282
1101	60-61	0.2815722912837444
1101	62-63	0.28764050526636
1101	64-65	0.3899579882247082
1101	66-67	0.40956600724446446
1101	68-69	0.3517680149795339
1101	70-71	0.41672550968477395
1101	72-73	0.4399531988898744
1101	74-75	0.41152635889429945
1101	76-77	0.3563069832166903
1101	78-79	0.2762278424980238
1101	80-81	0.27035700009975727
1101	82-83	0.2665983264250471
1101	84-85	0.29468367944870977
1101	86-87	0.2850070132073981
1101	88-89	0.2295651268740997
1101	90-91	0.2759363431264674
1101	92-93	0.36162619970348686
1101	94-95	0.3985586070195595
1101	96-97	0.3461694524509227
1101	98-99	0.3379171279179545
1101	100	0.31860783227055833
1102	1	0.1928586437994113
1102	2	0.21516763697231056
1102	3	0.1881564425529092
1102	4	0.12628294341487845
1102	5	0.10750619247247073
1102	6	0.13121544722493184
1102	7	0.14184453127039376
1102	8	0.0511753214641999
1102	9	0.12779564403807342
1102	10-11	0.15591454263265092
1102	12-13	0.1586405102221562
1102	14-15	0.22512675495566015
1102	16-17	0.2550080709374143
1102	18-19	0.24527818308594362
1102	20-21	0.2636041565155338
1102	22-23	0.2730529434333775
1102	24-25	0.23672947209535522
1102	26-27	0.2994876819059016
1102	28-29	0.24184780488857172
1102	30-31	0.21478346504131451
1102	32-33	0.2769827908769287
1102	34-35	0.23108750072729833
1102	36-37	0.3004987817227587
1102	38-39	0.3284527105253403
1102	40-41	0.3080851432093965
1102	42-43	0.2901448873324668
1102	44-45	0.23753568881277687
1102	46-47	0.26904320886117006
1102	48-49	0.21406093689808614
1102	50-51	0.2804011575622951
1102	52-53	0.3150610494048536
1102	54-55	0.2999261329975482
1102	56-57	0.2817391621527463
1102	58-59	0.2586392006864031
1102	60-61	0.3317754367407204
1102	62-63	0.3008629498682467
1102	64-65	0.36248062252043667
1102	66-67	0.3763250503516531
1102	68-69	0.3704932462199295
1102	70-71	0.4091164683733055
1102	72-73	0.43133352362223576
1102	74-75	0.4019559289324732
1102	76-77	0.36852956821162053
1102	78-79	0.4035623991009061
1102	80-81	0.355593856209957
1102	82-83	0.3011264262542923
1102	84-85	0.29206874489472057
1102	86-87	0.2921739470561562
1102	88-89	0.2661546209041319
1102	90-91	0.24187895175966645
1102	92-93	0.2872846920128893
1102	94-95	0.272106012523615
1102	96-97	0.25416450931010104
1102	98-99	0.25498141160330334
1102	100	0.2598334961173947
1103	1	0.15239010044025747
1103	2	0.20044252833827159
1103	3	0.1848860854775296
1103	4	0.1425157036642659
1103	5	0.14815106935388656
1103	6	0.12168902868472031
1103	7	0.12839784067275417
1103	8	0.015394730746905338
1103	9	0.10028950385292745
1103	10-11	0.12784161612594147
1103	12-13	0.16433041512800628
1103	14-15	0.23040448025322746
1103	16-17	0.1997810415275012
1103	18-19	0.26318831596584147
1103	20-21	0.21586091071920066
1103	22-23	0.2273652426965569
1103	24-25	0.20878060881680938
1103	26-27	0.24602965558182888
1103	28-29	0.262562270535021
1103	30-31	0.30773831106436234
1103	32-33	0.33540712490299995
1103	34-35	0.30975334870261406
1103	36-37	0.27083161124602384
1103	38-39	0.24820899240706495
1103	40-41	0.2867509911847108
1103	42-43	0.31084297915185033
1103	44-45	0.32378387824536503
1103	46-47	0.3608893578630017
1103	48-49	0.302399623213347
1103	50-51	0.3079705888866897
1103	52-53	0.34906539476664733
1103	54-55	0.3815997270525884
1103	56-57	0.3854834155692046
1103	58-59	0.39684059875933997
1103	60-61	0.3412558826126215
1103	62-63	0.373228652998165
1103	64-65	0.4020155428554659
1103	66-67	0.422289531742166
1103	68-69	0.3695794607170555
1103	70-71	0.45261637390891707
1103	72-73	0.4071997620503467
1103	74-75	0.3490089549416666
1103	76-77	0.3884388824001732
1103	78-79	0.4042910344053965
1103	80-81	0.35810503449574327
1103	82-83	0.3988759696764177
1103	84-85	0.33127965238259094
1103	86-87	0.3606178037221923
1103	88-89	0.3314770593448344
1103	90-91	0.35509766830352163
1103	92-93	0.40747299102415724
1103	94-95	0.44693062364522973
1103	96-97	0.4049406916893403
1103	98-99	0.3957742826909083
1103	100	0.3213827120629844
1104	1	0.10345809623306224
1104	2	0.11140745498521198
1104	3	0.14449750169563202
1104	4	0.05944422855990439
1104	5	0.04475582902298214
1104	6	0.09449016564803969
1104	7	0.04432333390883514
1104	8	0.032463738306034884
1104	9	0.0599140220810952
1104	10-11	0.08643034766939905
1104	12-13	0.1457443039865609
1104	14-15	0.15829875067143462
1104	16-17	0.13639096921168203
1104	18-19	0.14099633148444468
1104	20-21	0.15076894188742074
1104	22-23	0.18565144518812104
1104	24-25	0.19595537871276036
1104	26-27	0.17266986346705693
1104	28-29	0.144182061121235
1104	30-31	0.20999003707221675
1104	32-33	0.2780687254591072
1104	34-35	0.24248626422740927
1104	36-37	0.2715805185019917
1104	38-39	0.2975890125696097
1104	40-41	0.2917345772776585
1104	42-43	0.30589942697855577
1104	44-45	0.2320853664153404
1104	46-47	0.26923288164860537
1104	48-49	0.32087582304811946
1104	50-51	0.3132022033090891
1104	52-53	0.2900647687817255
1104	54-55	0.309474344455694
1104	56-57	0.27275472180321714
1104	58-59	0.2783643989245661
1104	60-61	0.2684540177598578
1104	62-63	0.25951912820079315
1104	64-65	0.2666520348650607
1104	66-67	0.29455530601637037
1104	68-69	0.2665301498463961
1104	70-71	0.2338867775600093
1104	72-73	0.25251415570023994
1104	74-75	0.25216189907251163
1104	76-77	0.23283740915192652
1104	78-79	0.26679017003667127
1104	80-81	0.28062202161574135
1104	82-83	0.2834684607688729
1104	84-85	0.22256062862792447
1104	86-87	0.16707654876799438
1104	88-89	0.19047804927448908
1104	90-91	0.19257050892849037
1104	92-93	0.17070929669405643
1104	94-95	0.20453725035076076
1104	96-97	0.22768023100810808
1104	98-99	0.2875835247032299
1104	100	0.25555093459334444
1105	1	0.044427553016888055
1105	2	0.10365023302934873
1105	3	0.10892537686576276
1105	4	0.11081170301609689
1105	5	0.07076058491039561
1105	6	0.0637114513835968
1105	7	0.06468419272180626
1105	8	-0.015130011602245474
1105	9	0.05879488818921175
1105	10-11	0.0806731041895219
1105	12-13	0.09224471429536862
1105	14-15	0.11270935310398045
1105	16-17	0.12000644272543326
1105	18-19	0.12748134809103107
1105	20-21	0.13191801603520048
1105	22-23	0.1692708609353133
1105	24-25	0.1413546397713148
1105	26-27	0.17058865196174366
1105	28-29	0.12603846403060714
1105	30-31	0.16048367088315985
1105	32-33	0.15110360121861532
1105	34-35	0.18202460394398656
1105	36-37	0.11563347518571021
1105	38-39	0.04779966308013428
1105	40-41	0.07006779752355641
1105	42-43	0.09554869884227912
1105	44-45	0.08604705492744813
1105	46-47	0.08554267259685844
1105	48-49	0.1384173749080233
1105	50-51	0.12380435961294722
1105	52-53	0.08595074028357175
1105	54-55	0.13565758855931165
1105	56-57	0.20043465066115118
1105	58-59	0.14334192502862209
1105	60-61	0.18662714994835738
1105	62-63	0.1734453239507019
1105	64-65	0.147072210191439
1105	66-67	0.1753778193576636
1105	68-69	0.17493954958121805
1105	70-71	0.10161480281700364
1105	72-73	0.11904354056161282
1105	74-75	0.089512684817187
1105	76-77	0.13671349940248234
1105	78-79	0.16765476801489854
1105	80-81	0.16435931302051188
1105	82-83	0.13995294848312057
1105	84-85	0.18202016741423677
1105	86-87	0.10684037272753244
1105	88-89	0.10165012016770447
1105	90-91	0.13708095460977887
1105	92-93	0.0973492528587272
1105	94-95	0.14561539192092
1105	96-97	0.20658203066357572
1105	98-99	0.24142184145127032
1105	100	0.15748612231876535
1106	1	0.05712934824580884
1106	2	0.1098288449557856
1106	3	0.071404907665638
1106	4	0.027073296475684572
1106	5	0.05728591520819748
1106	6	0.04523666085126621
1106	7	0.02645468900596626
1106	8	0.03404653788257406
1106	9	0.061640341954039
1106	10-11	0.061846481513420315
1106	12-13	0.054984701162425154
1106	14-15	0.1076212810876882
1106	16-17	0.07146626528103894
1106	18-19	0.11546475014046109
1106	20-21	0.13268193099511905
1106	22-23	0.11032035915050642
1106	24-25	0.08617047628928987
1106	26-27	0.09179173212735492
1106	28-29	0.10824854198714462
1106	30-31	0.13122420178720517
1106	32-33	0.09251928871823623
1106	34-35	-0.009948669416679934
1106	36-37	0.03476014018249174
1106	38-39	0.09947506741858803
1106	40-41	0.0773577726939223
1106	42-43	0.011807826380760389
1106	44-45	-0.01592138577583313
1106	46-47	0.004991606083237343
1106	48-49	-0.04815876432423494
1106	50-51	-0.06047915796762027
1106	52-53	-0.022538883586186387
1106	54-55	-0.006941286246373579
1106	56-57	-0.010212385666079626
1106	58-59	-0.15311954942012562
1106	60-61	-0.16924195326024716
1106	62-63	-0.14235932884788127
1106	64-65	-0.13641769725925457
1106	66-67	-0.15528307654216178
1106	68-69	-0.11597864381484868
1106	70-71	-0.11874862025192812
1106	72-73	-0.09343786707821522
1106	74-75	-0.062675800986149
1106	76-77	-0.09645052013440392
1106	78-79	-0.08566711540741778
1106	80-81	-0.027774108216519267
1106	82-83	0.032624252871535475
1106	84-85	0.041744162892506154
1106	86-87	0.011209458866481725
1106	88-89	-0.06375236007767171
1106	90-91	-0.04414145070555975
1106	92-93	0.0036535116853073646
1106	94-95	0.01644178619791603
1106	96-97	-0.014232683367112031
1106	98-99	-0.09107490531381046
1106	100	-0.007688671211035114
1107	1	0.09692878601195076
1107	2	0.10607367858074923
1107	3	0.08614892405615393
1107	4	0.07103534072008699
1107	5	0.08242247931624291
1107	6	0.05889024657005848
1107	7	0.054568474659198785
1107	8	0.06128627348387283
1107	9	0.03531368443716332
1107	10-11	0.07045022696987502
1107	12-13	0.08434458492794761
1107	14-15	0.1142487223146631
1107	16-17	0.0975942512786574
1107	18-19	0.07155572856486003
1107	20-21	0.08933070684896194
1107	22-23	0.13501037664824622
1107	24-25	0.12224752390338978
1107	26-27	0.0935525189576012
1107	28-29	0.0752309572950054
1107	30-31	0.11341250880033016
1107	32-33	0.09701279641026872
1107	34-35	0.14222029517799228
1107	36-37	0.11980648675581307
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2216	12-13	-0.18337353489104657
2216	14-15	-0.23955048110706656
2216	16-17	-0.23170890704404457
2216	18-19	-0.20279256198226392
2216	20-21	-0.17947073993190088
2216	22-23	-0.19778187805731307
2216	24-25	-0.23125063839162863
2216	26-27	-0.235035976317306
2216	28-29	-0.2347228775610617
2216	30-31	-0.22690069617135578
2216	32-33	-0.2825139877455598
2216	34-35	-0.3099689904991436
2216	36-37	-0.25520224475793896
2216	38-39	-0.22329114705684816
2216	40-41	-0.17835427216508748
2216	42-43	-0.19275532649835014
2216	44-45	-0.17346435293293183
2216	46-47	-0.18484610006174762
2216	48-49	-0.24932635663457603
2216	50-51	-0.22063715108694737
2216	52-53	-0.18817461442156258
2216	54-55	-0.2016013890233097
2216	56-57	-0.22427491079093187
2216	58-59	-0.19955849773328538
2216	60-61	-0.2303954794849119
2216	62-63	-0.22104598881657722
2216	64-65	-0.2360823972716659
2216	66-67	-0.22883110180683275
2216	68-69	-0.24066603222861715
2216	70-71	-0.28031611461948813
2216	72-73	-0.22436119461739423
2216	74-75	-0.3005385165685013
2216	76-77	-0.23766985569753984
2216	78-79	-0.23724505575574995
2216	80-81	-0.2853626308419166
2216	82-83	-0.24231342049255744
2216	84-85	-0.2978941014207521
2216	86-87	-0.24813487845140259
2216	88-89	-0.2510074127614672
2216	90-91	-0.2277410694842743
2216	92-93	-0.21894548457147778
2216	94-95	-0.2861498515894496
2216	96-97	-0.2671322980705426
2216	98-99	-0.3229411361839354
2216	100	-0.3361901783402601
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10.0
3	4.0
4	7.0
5	22.0
6	70.0
7	181.0
8	368.0
9	622.0
10	1117.0
11	1731.0
12	2761.0
13	4283.0
14	5194.0
15	6561.0
16	8951.0
17	12911.0
18	15858.0
19	8809.0
20	5949.0
21	5616.0
22	5792.0
23	6223.0
24	7016.0
25	8292.0
26	10195.0
27	13217.0
28	16859.0
29	21560.0
30	27410.0
31	35259.0
32	45087.0
33	58878.0
34	83721.0
35	131506.0
36	252587.0
37	579246.0
38	1017805.0
39	490450.0
40	1531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.442680737006384	19.452408237065132	13.43039867959243	23.674512346336048
2	15.8212911397217	20.011749886388966	39.89691202618151	24.270046947707822
3	18.44641941961902	25.46509582530858	29.931045589119076	26.15743916595332
4	12.467035933338448	16.642265480755206	38.24840960259247	32.642288983313875
5	12.741057756406867	38.00500612893114	35.292068537588996	13.961867577072995
6	30.985808406576847	37.83419225696931	17.477010890451293	13.702988446002548
7	27.65658478594435	32.68043018481912	22.026547877413915	17.63643715182262
8	25.850415941937705	35.86901376183301	19.72946989637962	18.55110039984967
9	25.823290591201648	16.17137217172052	20.722227290823437	37.28310994625439
10-11	24.39183400670224	26.473437264031457	28.345046876636122	20.78968185263018
12-13	25.29541379011236	23.877240982246317	28.73701511549588	22.09033011214544
14-15	22.31981697887274	25.561115613298753	26.189697804333967	25.92936960349454
16-17	21.556651975923906	29.124319762625795	26.741678960789784	22.57734930066051
18-19	21.519828991332588	27.528735721559016	29.532741382912164	21.418693904196235
20-21	22.390976269145742	26.853060433174747	29.3425382880291	21.41342500965041
22-23	22.19573127512613	26.6092040816256	29.336174265659544	21.85889037758873
24-25	21.847732797768625	26.74631481037661	29.42088408610114	21.98506830575363
26-27	21.658698463217036	26.791169593563737	29.454641029943364	22.095490913275867
28-29	21.7585583543048	26.83973318038837	29.504991592116653	21.89671687319018
30-31	21.923513015665527	27.100193259272793	29.150766945354267	21.825526779707413
32-33	21.75495033206719	26.88094645154121	29.46460527909837	21.89949793729323
34-35	21.876509069540344	26.67798162759145	29.414951994953203	22.030557307915
36-37	22.100354471462083	26.943098056724768	28.976482018961036	21.980065452852116
38-39	21.936820044335843	27.19208993841937	29.0378493383505	21.833240678894285
40-41	21.843519758628684	26.956927937308738	29.145894141071587	22.05365816299099
42-43	21.895293684965324	26.97269077457954	28.982163819387253	22.149851721067883
44-45	21.972160417408656	27.090152499665727	28.865114864700068	22.07257221822555
46-47	22.092078466132726	27.202593695287508	28.535215014841402	22.170112823738357
48-49	22.118698864656558	27.26095670251179	28.308702569909407	22.31164186292225
50-51	22.198094788007154	27.319357488714086	28.117557900796747	22.364989822482016
52-53	22.19797121845207	27.319880080343122	28.103107933918263	22.379040767286536
54-55	22.142432954151715	27.613612204436667	27.998387069710105	22.245567771701516
56-57	22.03064458159118	27.361483121922802	28.14103675045947	22.466835546026545
58-59	21.98352413019856	26.994494957155236	28.429635120343583	22.592345792302627
60-61	22.1934705045079	27.326805106129807	28.10382786787431	22.375896521487977
62-63	21.955768856605488	27.313677929826834	28.20079123159738	22.529761981970296
64-65	22.009953487954178	27.10117190726343	28.313218661908678	22.575655942873713
66-67	21.925669528729806	27.166837413606338	28.1821783738475	22.725314683816354
68-69	22.187921126063557	27.312701874843082	28.07766854070915	22.421708458384213
70-71	22.202616134105643	27.103556431493832	28.146042778364695	22.547784656035834
72-73	22.014342394870994	27.260382097545012	28.10367427537246	22.621601232211535
74-75	22.076616491438696	27.358631407501715	27.93668846260047	22.62806363845913
76-77	22.104174751879714	27.255078953725338	27.85594491676104	22.784801377633908
78-79	22.107045094117865	27.367098196435723	27.913949086606266	22.61190762284015
80-81	22.037784074317134	27.332976690708943	27.9222318640141	22.707007370959822
82-83	21.951096450682613	27.32414541470908	27.981290098508634	22.743468036099674
84-85	21.95300137300214	27.35358589246349	27.78264474148474	22.91076799304963
86-87	22.04276726342976	27.283660346653534	27.858542108836332	22.815030281080375
88-89	21.780091196255803	27.32405911635117	27.84226285871872	23.05358682867431
90-91	21.827185222277862	27.29620655966697	27.824466167850808	23.05214205020436
92-93	21.89298133207263	27.363401490033613	27.734112839738103	23.009504338155658
94-95	21.704109641115938	27.135208549145577	27.87720236485582	23.28347944488267
96-97	21.819230823062142	27.16285979842424	27.77734291776325	23.24056646075037
98-99	21.677782640902514	27.254788358750698	27.794848631930826	23.27258036841597
100	21.729270795211182	27.086156316276384	27.724413968612062	23.460158919900376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	69.5
2	250.0
3	465.0
4	332.0
5	218.5
6	573.0
7	1344.5
8	2306.0
9	2630.0
10	1978.0
11	1371.5
12	1349.5
13	1523.0
14	1714.5
15	1931.0
16	2150.5
17	2299.5
18	2568.0
19	2978.0
20	3392.5
21	4001.0
22	5037.0
23	6588.0
24	8626.0
25	11375.5
26	15104.5
27	19945.5
28	26210.0
29	33754.0
30	40918.5
31	47569.0
32	54550.5
33	61971.0
34	69250.5
35	75777.0
36	81456.5
37	87256.0
38	92875.0
39	95980.5
40	98812.5
41	101970.5
42	103875.5
43	105197.0
44	103964.5
45	102455.5
46	103181.0
47	101696.5
48	98957.5
49	96513.5
50	94205.5
51	91882.5
52	86233.5
53	80912.0
54	77842.0
55	74255.0
56	69043.0
57	62607.0
58	57031.5
59	52703.5
60	47328.5
61	40855.0
62	35528.5
63	30298.5
64	24787.5
65	20024.0
66	15886.0
67	12003.5
68	9087.0
69	7070.0
70	5160.0
71	3744.0
72	2871.0
73	2053.0
74	1391.5
75	952.5
76	602.0
77	374.5
78	249.0
79	150.5
80	88.0
81	39.0
82	14.5
83	10.5
84	11.5
85	8.0
86	4.0
87	2.0
88	1.5
89	1.0
90	1.0
91	1.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4190825525744397E-4
2	4.8381651051488794E-4
3	2.764665774370788E-4
4	0.012337321018129642
5	2.073499330778091E-4
6	0.003974207050658008
7	6.566081214130622E-4
8	0.14075604623765275
9	9.330746988501409E-4
10-11	0.0
12-13	2.073499330778091E-4
14-15	1.382332887185394E-4
16-17	0.0
18-19	1.555124498083568E-4
20-21	0.0
22-23	2.764665774370788E-4
24-25	5.356539937843402E-4
26-27	0.006203218831244455
28-29	0.008414951450741085
30-31	0.013667816422045584
32-33	0.017089090317829434
34-35	0.02536580847985198
36-37	0.03602705087226933
38-39	0.0411589617159451
40-41	0.05244225390759589
42-43	0.06844275707676682
44-45	0.10583486167513173
46-47	0.1128502010775976
48-49	0.1094807646650832
50-51	0.1112605182573344
52-53	0.10564479090314374
54-55	0.11291931772195687
56-57	0.09807651834580369
58-59	0.089246867028907
60-61	0.09068103739936186
62-63	0.08973068353942189
64-65	0.0
66-67	1.7279161089817424E-5
68-69	1.2095412762872198E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	6.91166443592697E-5
78-79	0.0
80-81	2.073499330778091E-4
82-83	1.382332887185394E-4
84-85	0.0
86-87	7.084456046825145E-4
88-89	3.283040607065311E-4
90-91	2.073499330778091E-4
92-93	0.00114042463192795
94-95	0.001261378759556672
96-97	2.591874163472614E-4
98-99	1.7279161089817424E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2893659.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.71141715913386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25773299810574	45.53457306659739
2	13.962002057988322	16.67381858522452
3	4.316016693505246	7.731464197550321
4	1.8181199660930771	4.342500789629361
5	0.9903885715780263	2.9568752573567116
6	0.5836858257377264	2.0911624698299334
7	0.4089631093155994	1.7093836776128153
8	0.28017078877637147	1.3383515875543597
9	0.21232226588247163	1.1410257051252717
>10	1.0925216378117935	11.586846035450284
>50	0.05899577164390279	2.3591931464195417
>100	0.01844276540399022	1.7590650422745033
>500	3.4775354063959604E-4	0.1260080722776205
>1k	1.7387677029307997E-4	0.20768317539961068
>5k	1.1591784686205332E-4	0.4420491916979216
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7148	0.2470228869400299	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5625	0.19439056226044604	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3274	0.1131439468161245	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.455832217963485E-5	0.0
5	0.0	0.0	0.0	3.455832217963485E-5	0.0
6	0.0	0.0	0.0	3.455832217963485E-5	0.0
7	0.0	0.0	0.0	3.455832217963485E-5	0.0
8	0.0	0.0	0.0	3.455832217963485E-5	0.0
9	0.0	0.0	0.0	6.91166443592697E-5	0.0
10-11	0.0	0.0	0.0	6.91166443592697E-5	0.0
12-13	0.0	0.0	0.0	6.91166443592697E-5	0.0
14-15	0.0	3.455832217963485E-5	0.0	6.91166443592697E-5	0.0
16-17	0.0	6.91166443592697E-5	0.0	1.2095412762872197E-4	0.0
18-19	3.455832217963485E-5	6.91166443592697E-5	0.0	1.382332887185394E-4	0.0
20-21	3.455832217963485E-5	6.91166443592697E-5	0.0	1.9007077198799168E-4	0.0
22-23	3.455832217963485E-5	6.91166443592697E-5	0.0	2.937457385268962E-4	0.0
24-25	3.455832217963485E-5	8.639580544908712E-5	0.0	4.4925818833525306E-4	0.0
26-27	3.455832217963485E-5	1.382332887185394E-4	0.0	6.566081214130622E-4	0.0
28-29	3.455832217963485E-5	1.382332887185394E-4	0.0	0.0017624744311613774	0.0
30-31	3.455832217963485E-5	1.382332887185394E-4	0.0	0.005874914770537925	0.0
32-33	3.455832217963485E-5	1.382332887185394E-4	0.0	0.013581420616596496	0.0
34-35	3.455832217963485E-5	1.382332887185394E-4	0.0	0.025106621063504718	0.0
36-37	3.455832217963485E-5	1.9007077198799168E-4	0.0	0.04753497215808773	0.0
38-39	3.455832217963485E-5	2.4190825525744394E-4	0.0	0.08719064685921873	0.0
40-41	3.455832217963485E-5	2.937457385268962E-4	0.0	0.13671272254263547	0.0
42-43	3.455832217963485E-5	3.1102489961671367E-4	0.0	0.1874443395023394	0.0
44-45	3.455832217963485E-5	3.1102489961671367E-4	0.0	0.24698832861785025	0.0
46-47	3.455832217963485E-5	3.4558322179634854E-4	0.0	0.31987183009470016	0.0
48-49	3.455832217963485E-5	3.8014154397598336E-4	0.0	0.4041422987297397	0.0
50-51	3.455832217963485E-5	3.8014154397598336E-4	0.0	0.49891849730738835	0.0
52-53	3.455832217963485E-5	3.8014154397598336E-4	0.0	0.5950251912889528	0.0
54-55	3.455832217963485E-5	3.8014154397598336E-4	0.0	0.6995468367212585	0.0
56-57	3.455832217963485E-5	3.8014154397598336E-4	0.0	0.8240086340512134	0.0
58-59	3.455832217963485E-5	3.8014154397598336E-4	0.0	0.9639007222343752	0.0
60-61	3.455832217963485E-5	3.8014154397598336E-4	0.0	1.115058823448098	0.0
62-63	3.455832217963485E-5	3.8014154397598336E-4	0.0	1.2689470321140122	0.0
64-65	3.455832217963485E-5	3.8014154397598336E-4	0.0	1.429833301021302	0.0
66-67	3.455832217963485E-5	4.4925818833525306E-4	0.0	1.6123876379352233	0.0
68-69	3.455832217963485E-5	4.4925818833525306E-4	0.0	1.8232970781975348	0.0
70-71	3.455832217963485E-5	4.4925818833525306E-4	0.0	2.0601598184167518	0.0
72-73	3.455832217963485E-5	4.6653734942507047E-4	0.0	2.303761431460998	0.0
74-75	3.455832217963485E-5	4.838165105148879E-4	0.0	2.5429741375884305	0.0
76-77	3.455832217963485E-5	4.838165105148879E-4	0.0	2.8000707754438237	0.0
78-79	3.455832217963485E-5	4.838165105148879E-4	0.0	3.096788529678169	0.0
80-81	3.455832217963485E-5	4.838165105148879E-4	0.0	3.4176279927938986	0.0
82-83	3.455832217963485E-5	4.838165105148879E-4	0.0	3.7578719538134937	0.0
84-85	3.455832217963485E-5	4.838165105148879E-4	0.0	4.0857785938149584	0.0
86-87	3.455832217963485E-5	4.838165105148879E-4	0.0	4.4291673621529	0.0
88	3.455832217963485E-5	4.838165105148879E-4	0.0	4.712372812415008	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4460	0.0	56.25049	1
GTATCAA	8590	0.0	43.480434	1
TCAACGC	10435	0.0	35.16965	4
ATCAACG	10525	0.0	34.780106	3
CAACGCA	10640	0.0	34.443645	5
TATCAAC	10860	0.0	33.880543	2
AACGCAG	10980	0.0	33.462196	6
ACGCAGA	12795	0.0	28.530684	7
CGCAGAG	13125	0.0	27.705336	8
GTACATG	10360	0.0	27.027534	1
TACATGG	10515	0.0	25.282066	2
GCAGAGT	14525	0.0	24.803278	9
ACATGGG	10935	0.0	23.708616	3
GAGTACT	8980	0.0	21.680937	12-13
GTGGTAT	2115	0.0	21.324594	1
CAGAGTA	13930	0.0	21.209448	10-11
AGAGTAC	13310	0.0	20.803463	10-11
CATGGGG	7225	0.0	20.383202	4
GAGTACA	7860	0.0	19.605196	1
TGGTATC	2160	0.0	19.386957	2
>>END_MODULE