FastQCFastQC Report
Wed 25 May 2016
SRR1032971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584312
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51350.040.2732051
TCAACGC55650.034.543164
ATCAACG57700.033.47883
CAACGCA58450.032.887945
GGTATCA31400.032.7807121
AACGCAG59550.032.4382866
TATCAAC66750.029.4326212
GTACATG122850.028.081791
TACATGG121700.026.6478652
ACGCAGA72050.026.4191537
ACATGGG124400.025.1627313
CGCAGAG75950.024.6912448
GAGTACA86050.020.9195231
GCAGAGT89100.019.9393949
CCGATCG1909.049951E-619.7894739
CATGGGG87800.019.646064
CATGGGA42400.018.733754
AGTACAT90400.018.509112
TAGCGCG1300.0080415118.0771754
GTATAAG16850.017.294011
Produced by FastQC (version 0.11.5)