FastQCFastQC Report
Wed 25 May 2016
SRR1032971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584312
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68390.1908037023562681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56950.15888683797615832No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59650.050.3278541
GTATCAA107100.042.2430081
TCAACGC125200.035.278224
ATCAACG126000.035.0542343
CAACGCA127800.034.594425
TATCAAC132100.033.7562
AACGCAG132250.033.5015036
ACGCAGA151850.028.8048137
CGCAGAG156100.027.8996648
GTACATG127500.026.4564911
TACATGG126750.025.3465122
CGCGTAT1700.024.8685812-13
GCAGAGT172250.024.6800929
GTGGTAT29400.023.6500661
ACATGGG132300.023.4299323
TGGTATC30850.021.1936172
GAGTACT103600.021.10655212-13
CAGAGTA165700.020.99229610-11
CCGCGTA2500.020.66855210-11
ACGACGG1857.1426675E-620.3388926
Produced by FastQC (version 0.11.5)