FastQCFastQC Report
Wed 25 May 2016
SRR1032970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066580
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54500.036.1359441
GGTATCA34450.034.927911
TCAACGC56100.033.9323584
CAACGCA57900.032.71435
ATCAACG58250.032.5992243
AACGCAG60050.031.6212866
GTACATG145750.029.5076351
TACATGG148100.027.8968812
TATCAAC69600.027.8909112
ACATGGG150350.026.8854223
ACGCAGA75900.024.4608657
CGCAGAG80850.022.9051278
GAGTACA100750.022.0200751
CATGGGG107150.021.9769484
AGTACAT103300.020.0204682
CCTATAC15900.018.9192833
GCAGAGT95550.018.8891189
CATGGGA49300.018.1145924
GTATAGG17950.017.8059981
TACGCGC2203.196928E-517.0914336
Produced by FastQC (version 0.11.5)