FastQCFastQC Report
Wed 25 May 2016
SRR1032970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066580
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58580.1440522502938587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48270.11869925096764358No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53850.049.9918251
GTATCAA96600.043.3341871
TCAACGC113550.036.130954
ATCAACG114650.035.7842943
CAACGCA115650.035.5107465
AACGCAG119200.034.53216
TATCAAC123400.033.4371382
GTACATG134450.029.6672251
ACGCAGA140250.029.0458987
TACATGG133000.028.2290272
CGCAGAG146250.027.7899448
ACATGGG137900.026.1686383
GCAGAGT164850.024.3345999
GTGGTAT24700.023.2056081
CATGGGG95750.021.227314
GAGTACT99100.020.19230512-13
GAGTACA93750.020.1458131
ACACCGT7750.020.0310366
CAGAGTA159650.020.00736410-11
AGAGTAC149300.019.58526610-11
Produced by FastQC (version 0.11.5)