FastQCFastQC Report
Wed 25 May 2016
SRR1032969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2967164
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44800.034.5177351
TCAACGC45650.032.6394584
ATCAACG46500.031.840663
CAACGCA47300.031.7979155
AACGCAG49400.030.3505256
GGTATCA28300.030.0619091
TATCAAC54450.027.6233622
GTACATG94750.025.3989161
ACGCAGA58700.024.9815257
TACATGG93500.024.531982
CGCAGAG61200.023.5770458
GTCGTAC2202.4538167E-923.5014381
ACATGGG94550.023.2653063
CGTACAC3202.0008883E-1120.5637593
GCAGAGT73200.019.0695539
ACACCGT4750.018.8001986
GCGTATG2654.4792432E-717.7369331
GAGTACA70000.017.5253581
CATGGGG61700.017.445154
CGTAAGG3008.673669E-817.2343883
Produced by FastQC (version 0.11.5)