FastQCFastQC Report
Wed 25 May 2016
SRR1032969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2967164
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69450.2340618853558482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55980.1886650013278673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29790.10039889942045671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47950.052.3137471
GTATCAA89800.042.5805471
ATCAACG106200.035.800573
TCAACGC106150.035.7731064
CAACGCA109900.034.5495385
AACGCAG111700.033.9085436
TATCAAC112850.033.8571362
ACGCAGA128950.029.334537
CGCAGAG130600.028.8918678
GTACATG94000.025.6361451
GCAGAGT144950.025.502099
GTGGTAT23600.024.482531
TACATGG93900.024.103232
ACATGGG93750.023.489713
TGGTATC25350.022.274042
CGCGCGT1954.6331843E-721.7146247
GAGTACT95150.021.2261712-13
CCGATCG1354.0968478E-420.8754929
CAGAGTA143150.020.81863410-11
AGAGTAC135050.020.79785510-11
Produced by FastQC (version 0.11.5)