##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032969_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2967164 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83681926580398 34.0 31.0 34.0 31.0 34.0 2 32.87677762334674 34.0 31.0 34.0 31.0 34.0 3 32.959582618284664 34.0 31.0 34.0 31.0 34.0 4 36.37242633032754 37.0 37.0 37.0 35.0 37.0 5 36.35519169146026 37.0 37.0 37.0 35.0 37.0 6 36.41481630270521 37.0 37.0 37.0 35.0 37.0 7 36.37840105905841 37.0 37.0 37.0 35.0 37.0 8 36.31397523021983 37.0 37.0 37.0 35.0 37.0 9 38.15340506962204 39.0 39.0 39.0 37.0 39.0 10-11 38.167308075994455 39.0 39.0 39.0 37.0 39.0 12-13 38.16231509279568 39.0 39.0 39.0 37.0 39.0 14-15 39.704789657733784 41.0 40.0 41.0 37.0 41.0 16-17 39.71913635377081 41.0 40.0 41.0 37.0 41.0 18-19 39.749738302298084 41.0 40.0 41.0 37.0 41.0 20-21 39.76694547385989 41.0 40.0 41.0 37.0 41.0 22-23 39.7340593576897 41.0 40.0 41.0 37.0 41.0 24-25 39.71879663544044 41.0 40.0 41.0 37.0 41.0 26-27 39.64643241829572 41.0 40.0 41.0 37.0 41.0 28-29 39.58733777438658 41.0 40.0 41.0 37.0 41.0 30-31 39.47609316505593 41.0 39.5 41.0 36.5 41.0 32-33 39.35297088398215 41.0 39.0 41.0 35.5 41.0 34-35 39.264302883157114 41.0 39.0 41.0 35.0 41.0 36-37 39.17875857215847 41.0 39.0 41.0 35.0 41.0 38-39 39.084805389927894 41.0 39.0 41.0 35.0 41.0 40-41 38.98210109046888 41.0 39.0 41.0 35.0 41.0 42-43 38.84866727959762 41.0 39.0 41.0 35.0 41.0 44-45 38.67782586334965 41.0 39.0 41.0 35.0 41.0 46-47 38.45222222297116 41.0 39.0 41.0 35.0 41.0 48-49 38.2468203644962 41.0 38.0 41.0 34.5 41.0 50-51 38.094983795974876 40.5 38.0 41.0 34.0 41.0 52-53 37.96089161232746 40.0 38.0 41.0 34.0 41.0 54-55 37.82022665413842 40.0 38.0 41.0 34.0 41.0 56-57 37.61514631479756 40.0 37.0 41.0 33.0 41.0 58-59 37.4525455957271 40.0 37.0 41.0 33.0 41.0 60-61 37.27071102237692 40.0 36.0 41.0 33.0 41.0 62-63 37.03800784183146 39.5 36.0 41.0 33.0 41.0 64-65 36.751654273238685 39.0 35.0 41.0 32.0 41.0 66-67 36.48065138967715 39.0 35.0 41.0 32.0 41.0 68-69 36.18869432225519 38.0 35.0 40.5 32.0 41.0 70-71 35.86268521052426 37.0 35.0 40.0 31.5 41.0 72-73 35.51897738042117 37.0 35.0 39.5 31.0 41.0 74-75 35.16815467564314 36.0 35.0 39.0 31.0 41.0 76-77 34.81682576359109 36.0 35.0 39.0 31.0 40.5 78-79 34.469301831647996 35.0 35.0 37.5 31.0 39.5 80-81 34.13187036510284 35.0 34.5 37.0 30.0 39.0 82-83 33.81577914129451 35.0 34.0 37.0 30.0 39.0 84-85 33.531761473245155 35.0 34.0 36.0 30.0 37.5 86-87 33.3806314716679 35.0 34.0 36.0 30.0 37.0 88-89 33.18830455613508 35.0 34.0 36.0 30.0 37.0 90-91 33.008847000031004 35.0 34.0 35.0 30.0 36.5 92-93 32.84672468390692 35.0 34.0 35.0 30.0 36.0 94-95 32.745444134533855 35.0 34.0 35.0 29.5 36.0 96-97 32.68359686218895 35.0 34.0 35.0 30.0 36.0 98-99 32.57241055769078 35.0 34.0 35.0 30.0 36.0 100 32.48783552240456 35.0 34.0 35.0 30.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15505519065070672 1101 2 0.17104313716911435 1101 3 0.15056712365261404 1101 4 0.0778112355405014 1101 5 0.09075606833174277 1101 6 0.12873906744609087 1101 7 0.12335562661107957 1101 8 0.05322167982158987 1101 9 0.1351899209187266 1101 10-11 0.17999030341486133 1101 12-13 0.19195802022054664 1101 14-15 0.25614379472705195 1101 16-17 0.224212409736829 1101 18-19 0.27620559242659226 1101 20-21 0.2130136051088769 1101 22-23 0.2062057740601233 1101 24-25 0.22014684139597307 1101 26-27 0.27598053313460014 1101 28-29 0.2515897815545003 1101 30-31 0.24885131931788607 1101 32-33 0.2705866749156627 1101 34-35 0.23368984511005664 1101 36-37 0.2513220974650068 1101 38-39 0.27230248928447054 1101 40-41 0.24708934476004885 1101 42-43 0.2098438476620217 1101 44-45 0.23137813613416824 1101 46-47 0.20872933944332317 1101 48-49 0.21729499508825256 1101 50-51 0.22885322042890976 1101 52-53 0.295048460807827 1101 54-55 0.232250247551697 1101 56-57 0.2507935681318756 1101 58-59 0.2430428045752322 1101 60-61 0.2921567713127686 1101 62-63 0.23343497153681625 1101 64-65 0.2812068244106598 1101 66-67 0.2542807448445643 1101 68-69 0.2157797087969442 1101 70-71 0.20631935074054297 1101 72-73 0.22656111266922352 1101 74-75 0.28915415022295576 1101 76-77 0.23972122396317985 1101 78-79 0.2559974481904348 1101 80-81 0.3083073070194118 1101 82-83 0.2849242035628379 1101 84-85 0.29712719716766856 1101 86-87 0.2817854621578775 1101 88-89 0.18764097788636747 1101 90-91 0.18158156275769954 1101 92-93 0.20509878675763815 1101 94-95 0.23001159541657046 1101 96-97 0.25526643413127204 1101 98-99 0.2232425477833928 1101 100 0.2434620990090508 1102 1 0.1931874405448113 1102 2 0.22775939738451711 1102 3 0.21073863965654738 1102 4 0.1380410317995171 1102 5 0.11542182319311678 1102 6 0.17552829793347513 1102 7 0.15603958139045204 1102 8 0.11636682164738232 1102 9 0.22561737084617306 1102 10-11 0.16198818690863703 1102 12-13 0.20911077167864534 1102 14-15 0.2906501282519969 1102 16-17 0.2874314014908421 1102 18-19 0.31063371409153717 1102 20-21 0.31035647378661935 1102 22-23 0.31759563819079517 1102 24-25 0.2771682550883625 1102 26-27 0.32779701115579485 1102 28-29 0.2924934938492356 1102 30-31 0.3173025088166739 1102 32-33 0.325356180747562 1102 34-35 0.3172607623661534 1102 36-37 0.3079669916300034 1102 38-39 0.34179920959643795 1102 40-41 0.3005606306478441 1102 42-43 0.29670893214294836 1102 44-45 0.2864338331563232 1102 46-47 0.2772063370384501 1102 48-49 0.25114697826476373 1102 50-51 0.23105260336096478 1102 52-53 0.2125579092367147 1102 54-55 0.19334526932743046 1102 56-57 0.19124708060166284 1102 58-59 0.2090465141558724 1102 60-61 0.18963986398055965 1102 62-63 0.2339176919864272 1102 64-65 0.2184446844275456 1102 66-67 0.21663509267276027 1102 68-69 0.19172765350316467 1102 70-71 0.24265036514169935 1102 72-73 0.26840778979806856 1102 74-75 0.25136782575145133 1102 76-77 0.23478388068981104 1102 78-79 0.17920994066610518 1102 80-81 0.19236291971088093 1102 82-83 0.2344209093350358 1102 84-85 0.2155727646210721 1102 86-87 0.21451520510439082 1102 88-89 0.14381658877491077 1102 90-91 0.10388047285634627 1102 92-93 0.184741651170377 1102 94-95 0.15502786784538358 1102 96-97 0.2238147250779292 1102 98-99 0.20892662712014243 1102 100 0.21323391181820028 1103 1 0.1629808403095936 1103 2 0.18873200516279098 1103 3 0.20010473337042356 1103 4 0.14915928280127844 1103 5 0.130430064728273 1103 6 0.10647288085976925 1103 7 0.1211604343902195 1103 8 0.029213422229439345 1103 9 0.08435732934966467 1103 10-11 0.096258824229416 1103 12-13 0.15367333873962252 1103 14-15 0.1720514669346187 1103 16-17 0.18415925952012202 1103 18-19 0.2225517285395 1103 20-21 0.19103035799221146 1103 22-23 0.19323030435970168 1103 24-25 0.17183514345098416 1103 26-27 0.23815682649197356 1103 28-29 0.23667664680854017 1103 30-31 0.2297154137706272 1103 32-33 0.3037441945897257 1103 34-35 0.3081686213351915 1103 36-37 0.30546175125101627 1103 38-39 0.3419536761311832 1103 40-41 0.3523154443031373 1103 42-43 0.34202952475543924 1103 44-45 0.28380134024045844 1103 46-47 0.35943827974527665 1103 48-49 0.326588047157955 1103 50-51 0.30034890386255597 1103 52-53 0.3218047101419188 1103 54-55 0.3199846856718409 1103 56-57 0.36173035667722075 1103 58-59 0.31862087902686653 1103 60-61 0.27336248344707315 1103 62-63 0.32736048359304704 1103 64-65 0.3132760050967889 1103 66-67 0.34029040187474635 1103 68-69 0.29848409531131637 1103 70-71 0.3276877517261241 1103 72-73 0.3010678095702488 1103 74-75 0.32397556301035024 1103 76-77 0.3777345881424381 1103 78-79 0.3814570493912086 1103 80-81 0.3441984848263999 1103 82-83 0.33618148802238323 1103 84-85 0.2987833859661464 1103 86-87 0.263974668902506 1103 88-89 0.27243732477499094 1103 90-91 0.24667935022181098 1103 92-93 0.25480583167868787 1103 94-95 0.329442497387582 1103 96-97 0.317809823160232 1103 98-99 0.3172282908591839 1103 100 0.25430929652570455 1104 1 0.07660935622330811 1104 2 0.13429429062573206 1104 3 0.1215150211731526 1104 4 0.09632093207825676 1104 5 0.0675964411889396 1104 6 0.05957105664298723 1104 7 0.07015361228196326 1104 8 -0.04424259115631202 1104 9 0.030266161587917395 1104 10-11 0.09057795674019786 1104 12-13 0.08032850485987808 1104 14-15 0.1757577149791274 1104 16-17 0.13428452421974413 1104 18-19 0.17654248615853874 1104 20-21 0.20035605981460236 1104 22-23 0.1471194898784205 1104 24-25 0.19406676524908306 1104 26-27 0.2078717331045894 1104 28-29 0.23410670829593982 1104 30-31 0.25826394732343516 1104 32-33 0.23556168335558425 1104 34-35 0.18975088453390043 1104 36-37 0.2625678306132855 1104 38-39 0.2224243993336117 1104 40-41 0.24282976075465257 1104 42-43 0.28997883959720383 1104 44-45 0.28339527856261526 1104 46-47 0.3218939836535384 1104 48-49 0.2548310992181868 1104 50-51 0.26038056579069746 1104 52-53 0.3163194199585959 1104 54-55 0.21241759253838666 1104 56-57 0.3272023325266318 1104 58-59 0.33592560212952804 1104 60-61 0.23394964613927982 1104 62-63 0.2201146575715498 1104 64-65 0.23909487347476244 1104 66-67 0.2399445919506107 1104 68-69 0.23253741726313137 1104 70-71 0.20878802923560613 1104 72-73 0.2422284295228181 1104 74-75 0.21496180429849687 1104 76-77 0.25539416743443155 1104 78-79 0.25666209240064575 1104 80-81 0.2701679825886316 1104 82-83 0.21676523285875504 1104 84-85 0.17873448738860986 1104 86-87 0.28243979090238724 1104 88-89 0.27070274877049627 1104 90-91 0.26777032640224974 1104 92-93 0.267541113926697 1104 94-95 0.26153378304551467 1104 96-97 0.2983717175252423 1104 98-99 0.32100995278732825 1104 100 0.30063087878323813 1105 1 0.08838486602969198 1105 2 0.1513415167333676 1105 3 0.11328330989527302 1105 4 0.08528772158635434 1105 5 0.09742845083725626 1105 6 0.08286387106870308 1105 7 0.09341021302420671 1105 8 0.054204821595234876 1105 9 0.10294832345280014 1105 10-11 0.11506595804799957 1105 12-13 0.065548044226162 1105 14-15 0.14878333846075265 1105 16-17 0.12774516660393687 1105 18-19 0.15525417218970006 1105 20-21 0.1444532835404715 1105 22-23 0.17042564761202073 1105 24-25 0.17470806140664052 1105 26-27 0.1634708882269038 1105 28-29 0.18217328192934445 1105 30-31 0.20359993343989657 1105 32-33 0.16196624920277713 1105 34-35 0.19797964566866 1105 36-37 0.15996748244226922 1105 38-39 0.16417309260966562 1105 40-41 0.1760997156309898 1105 42-43 0.16158890862514141 1105 44-45 0.08358387119819355 1105 46-47 0.07203139536576941 1105 48-49 0.07153973550506265 1105 50-51 0.13233239130678953 1105 52-53 0.10842337542387526 1105 54-55 0.13695008958282529 1105 56-57 0.09382400725522189 1105 58-59 0.11907996011342448 1105 60-61 0.1367551781177525 1105 62-63 0.09572371326591878 1105 64-65 0.08939362283060603 1105 66-67 0.1636406286904517 1105 68-69 0.16874284574299736 1105 70-71 0.16248458563368473 1105 72-73 0.11637337519687918 1105 74-75 0.1386320475851619 1105 76-77 0.15722726186932334 1105 78-79 0.17155736502635222 1105 80-81 0.17097277621935802 1105 82-83 0.1434298714148028 1105 84-85 0.15579382261580577 1105 86-87 0.10152621548049012 1105 88-89 0.08901358342823329 1105 90-91 0.1523976177702906 1105 92-93 0.10468520216466715 1105 94-95 0.10659621781758233 1105 96-97 0.1165801903362933 1105 98-99 0.12855536333898954 1105 100 0.1597810318658901 1106 1 0.12255438492042714 1106 2 0.1712160523193802 1106 3 0.13644783211586287 1106 4 0.028960894961024053 1106 5 0.05865179053766667 1106 6 0.08292741640516255 1106 7 0.1127933174062008 1106 8 0.05078095078958711 1106 9 0.11450981213608458 1106 10-11 0.09566202898131593 1106 12-13 0.09413942179290302 1106 14-15 0.18206549585001142 1106 16-17 0.14615774138989934 1106 18-19 0.1533788008988921 1106 20-21 0.13967970142714847 1106 22-23 0.1540976577307731 1106 24-25 0.14354158224052327 1106 26-27 0.15772309235124737 1106 28-29 0.17335495553792413 1106 30-31 0.18657764804859056 1106 32-33 0.1675308296593272 1106 34-35 0.18197667241580717 1106 36-37 0.15528357450440922 1106 38-39 0.14017064465099338 1106 40-41 0.11801384330610887 1106 42-43 0.15148883203629282 1106 44-45 0.1847944777927708 1106 46-47 0.21471777132924075 1106 48-49 0.16635677196796195 1106 50-51 0.17411222197518583 1106 52-53 0.17437609246778152 1106 54-55 0.1761241172660135 1106 56-57 0.1724589311450515 1106 58-59 0.1430281384734826 1106 60-61 0.1485858199716361 1106 62-63 0.13191205077360024 1106 64-65 0.16536476384147392 1106 66-67 0.15702796824369614 1106 68-69 0.2157579812388022 1106 70-71 0.21686875920777027 1106 72-73 0.21511566875710741 1106 74-75 0.20475005566461846 1106 76-77 0.2002669206392298 1106 78-79 0.1482563718796328 1106 80-81 0.15390878920100448 1106 82-83 0.15847185209785408 1106 84-85 0.2170505079405558 1106 86-87 0.1763843985939033 1106 88-89 0.17186201776730314 1106 90-91 0.15216154370987311 1106 92-93 0.20191751011874004 1106 94-95 0.21144743393735865 1106 96-97 0.13039928909620357 1106 98-99 0.13372840314303147 1106 100 0.1577102168593285 1107 1 0.08457656395341928 1107 2 0.10179358380617742 1107 3 0.08246220096309997 1107 4 0.06374819202985549 1107 5 0.06826781582824992 1107 6 0.05231243552321985 1107 7 0.021960471757921596 1107 8 0.0638074599841616 1107 9 0.06775867761609078 1107 10-11 0.08160129633788671 1107 12-13 0.04944449187102862 1107 14-15 0.0889943664838242 1107 16-17 0.09770432514608984 1107 18-19 0.12898501549155128 1107 20-21 0.12761912973984835 1107 22-23 0.13448805262321173 1107 24-25 0.11821146160770724 1107 26-27 0.12088937448929471 1107 28-29 0.09140550379225232 1107 30-31 0.10145046661128987 1107 32-33 0.08153795708601308 1107 34-35 0.12893981948155187 1107 36-37 0.12129102344197662 1107 38-39 0.11785127677109841 1107 40-41 0.13497725528718973 1107 42-43 0.08458471493086961 1107 44-45 0.06539507899002928 1107 46-47 -0.006490798521312513 1107 48-49 -0.03705440432838003 1107 50-51 0.013343283137714934 1107 52-53 -0.020083554504431333 1107 54-55 -0.00673830898917771 1107 56-57 0.015819159405737082 1107 58-59 -0.021859079843245866 1107 60-61 -0.01125972836697997 1107 62-63 0.03211162032631165 1107 64-65 0.011873069338626863 1107 66-67 0.024115679704337367 1107 68-69 0.007046541472121248 1107 70-71 -0.03472118436526017 1107 72-73 -0.00933093916257377 1107 74-75 -0.014068054670204333 1107 76-77 0.007104254996903592 1107 78-79 -0.025909556235191644 1107 80-81 0.002153594652796187 1107 82-83 0.035563930647661834 1107 84-85 0.016416025677443713 1107 86-87 -0.028391543240317674 1107 88-89 0.020726915762857345 1107 90-91 0.040295574412766655 1107 92-93 0.042741725949277054 1107 94-95 0.07250746856902168 1107 96-97 0.09366902870047511 1107 98-99 0.050495918261269424 1107 100 0.08057560133576658 1108 1 -0.050033732427827715 1108 2 -0.03295286926380214 1108 3 -0.014299722596796016 1108 4 -0.05075958319302032 1108 5 -0.05253754422604118 1108 6 -0.0730972416058151 1108 7 -0.022227491681789502 1108 8 -0.039596302730075195 1108 9 -0.023375339577889065 1108 10-11 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-0.1345872703521067 2214 32-33 -0.10031661963052585 2214 34-35 -0.15720036905262447 2214 36-37 -0.15076861134100739 2214 38-39 -0.12464665677914155 2214 40-41 -0.10440754397937013 2214 42-43 -0.12505899977076496 2214 44-45 -0.09724485306213637 2214 46-47 -0.09040834561355382 2214 48-49 -0.12843447350704906 2214 50-51 -0.1302254595595258 2214 52-53 -0.11573026362166416 2214 54-55 -0.06477593194155418 2214 56-57 -0.1812349395943471 2214 58-59 -0.09063134976074139 2214 60-61 -0.11174266176443126 2214 62-63 -0.1255851165190407 2214 64-65 -0.13212856430579478 2214 66-67 -0.09348225957212719 2214 68-69 -0.15459896111406124 2214 70-71 -0.16163721362158867 2214 72-73 -0.17555840946771895 2214 74-75 -0.16033801119965574 2214 76-77 -0.21022326331783603 2214 78-79 -0.16089368413775418 2214 80-81 -0.17038873386482578 2214 82-83 -0.13971532574026924 2214 84-85 -0.16242511220390554 2214 86-87 -0.12982525323513983 2214 88-89 -0.151075774811666 2214 90-91 -0.09097099816342791 2214 92-93 -0.11523498841227564 2214 94-95 -0.08470761022740447 2214 96-97 -0.044489989455776424 2214 98-99 -0.054888119562313875 2214 100 -0.08387934851864287 2215 1 -0.16435453694199254 2215 2 -0.21917721057364759 2215 3 -0.1633915342670349 2215 4 -0.09582757674706244 2215 5 -0.13604064663462623 2215 6 -0.08625616042765216 2215 7 -0.07130525063021054 2215 8 -0.0505572594460304 2215 9 -0.12960593393318476 2215 10-11 -0.17481063478893333 2215 12-13 -0.1658211585964935 2215 14-15 -0.22779031409251616 2215 16-17 -0.17349088661409695 2215 18-19 -0.18381898496673443 2215 20-21 -0.18996562576771936 2215 22-23 -0.1968014671227678 2215 24-25 -0.20625881385657152 2215 26-27 -0.24788754869699403 2215 28-29 -0.23677533137324502 2215 30-31 -0.20910049541044629 2215 32-33 -0.20779851678047123 2215 34-35 -0.16034108317223428 2215 36-37 -0.18129293341235098 2215 38-39 -0.2616703341166797 2215 40-41 -0.19837199535863448 2215 42-43 -0.17852904590635177 2215 44-45 -0.1714201594811371 2215 46-47 -0.13783020273434943 2215 48-49 -0.15695061285799738 2215 50-51 -0.11098950688840148 2215 52-53 -0.18787081669602657 2215 54-55 -0.1789036544631557 2215 56-57 -0.17926427267904899 2215 58-59 -0.0999999823465032 2215 60-61 -0.09802311709869826 2215 62-63 -0.1407117986956834 2215 64-65 -0.21113023482784143 2215 66-67 -0.14738176585971274 2215 68-69 -0.19394458302328133 2215 70-71 -0.2511511959979984 2215 72-73 -0.23435404156665385 2215 74-75 -0.17385460936373676 2215 76-77 -0.14764367582216664 2215 78-79 -0.18010194218458508 2215 80-81 -0.17229267939983828 2215 82-83 -0.15870198994028328 2215 84-85 -0.18245536438898569 2215 86-87 -0.2636105205794763 2215 88-89 -0.2398744324976363 2215 90-91 -0.2602162745071368 2215 92-93 -0.19906409175873563 2215 94-95 -0.19196994041586635 2215 96-97 -0.23938538927365016 2215 98-99 -0.23120689408532513 2215 100 -0.2346117727509025 2216 1 -0.2302019577100367 2216 2 -0.28185480417774045 2216 3 -0.2459882871939456 2216 4 -0.17377756858341797 2216 5 -0.16603004075488315 2216 6 -0.22062785466548718 2216 7 -0.19099507323898735 2216 8 -0.14150960707740268 2216 9 -0.22091171942000898 2216 10-11 -0.2499320604496731 2216 12-13 -0.2739124295321318 2216 14-15 -0.3344136379724816 2216 16-17 -0.3894409017751528 2216 18-19 -0.36530435008751283 2216 20-21 -0.3238973406410608 2216 22-23 -0.3869533658222082 2216 24-25 -0.3333114220386051 2216 26-27 -0.4068063320804711 2216 28-29 -0.40322778808191373 2216 30-31 -0.39813390598970955 2216 32-33 -0.4302423351167022 2216 34-35 -0.48459417815454486 2216 36-37 -0.4547379989341849 2216 38-39 -0.4167562170458794 2216 40-41 -0.41450534954699236 2216 42-43 -0.4044551738272659 2216 44-45 -0.36541439141924315 2216 46-47 -0.3761541650251701 2216 48-49 -0.45814315691081475 2216 50-51 -0.43978570523619 2216 52-53 -0.39360073584162336 2216 54-55 -0.34247358946955586 2216 56-57 -0.3500175988503287 2216 58-59 -0.36269119346113143 2216 60-61 -0.323136825026765 2216 62-63 -0.38102954289503543 2216 64-65 -0.3091232127201309 2216 66-67 -0.3608676633325487 2216 68-69 -0.3811032019813325 2216 70-71 -0.38667115165362276 2216 72-73 -0.3888586248708279 2216 74-75 -0.3239436548482786 2216 76-77 -0.4466594076484469 2216 78-79 -0.3598423107972124 2216 80-81 -0.4098190397445478 2216 82-83 -0.43461916529128786 2216 84-85 -0.37993982945063465 2216 86-87 -0.3074029252328927 2216 88-89 -0.3118482580645221 2216 90-91 -0.3241326156569926 2216 92-93 -0.3648450714237157 2216 94-95 -0.43528899079860395 2216 96-97 -0.39192956557548086 2216 98-99 -0.43628303552083736 2216 100 -0.43301231553046904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 6.0 4 14.0 5 22.0 6 55.0 7 153.0 8 301.0 9 629.0 10 1068.0 11 1786.0 12 2832.0 13 4305.0 14 5532.0 15 7458.0 16 9561.0 17 13909.0 18 17104.0 19 9354.0 20 6030.0 21 5723.0 22 5724.0 23 6147.0 24 6943.0 25 8162.0 26 10238.0 27 12971.0 28 16595.0 29 21625.0 30 27473.0 31 34965.0 32 44519.0 33 58041.0 34 81956.0 35 127559.0 36 239207.0 37 559545.0 38 1061348.0 39 556077.0 40 2217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89594391924911 20.02979289890973 13.940245690308883 23.134017491532276 2 15.376018695362188 20.00476551091231 40.84376505020132 23.77545074352418 3 18.340353509515154 25.70911193928744 30.03536712858954 25.915167422607865 4 12.252041023725795 16.956010423453645 39.03334635534728 31.75860219747328 5 12.427472539323904 38.14273754765512 35.81208512405162 13.617704788969357 6 30.46909124735091 38.41572252090962 17.61776656726398 13.497419664475494 7 27.143361717587787 32.92000574290781 22.315578176680695 17.621054362823703 8 25.267577350467015 36.48464803111737 19.824095714611158 18.42367890380445 9 25.245413670367995 16.688803636372214 21.210171569660005 36.855611123599786 10-11 24.00209425565961 26.947246596413276 28.451932552430538 20.598726595496576 12-13 24.965059192945716 24.172382287311002 29.051709698260392 21.810848821482892 14-15 21.901872818094205 25.963012773664513 26.61080403665526 25.524310371586022 16-17 20.999462786687893 29.724157478319363 27.143460893971483 22.132918841021258 18-19 20.90069644048458 28.122822517630077 30.0055659293004 20.970915112584947 20-21 21.85413411594371 27.30245109471536 29.937256585749893 20.906158203591037 22-23 21.580758955458034 27.035947204681115 30.057644405065187 21.325649434795665 24-25 21.332747136603665 27.208436919002565 29.988141817592943 21.470674126800823 26-27 21.06300808247049 27.268095989300896 30.081296565712602 21.587599362516016 28-29 21.197510073175348 27.3036010980431 30.120787100538287 21.378101728243266 30-31 21.379304649462142 27.579789730710974 29.76127335260378 21.279632267223107 32-33 21.17759069328921 27.370401500556103 30.02158844360114 21.43041936255355 34-35 21.30444969213369 27.199874594835126 30.085272141006648 21.410403572024535 36-37 21.553533998801765 27.442632932696924 29.567509577613986 21.436323490887318 38-39 21.362662564921447 27.729465536843552 29.617413580804698 21.290458317430307 40-41 21.35757885169082 27.4443893488277 29.70245163505001 21.495580164431473 42-43 21.365247718662534 27.410855243964917 29.53801251046783 21.685884526904722 44-45 21.438348630786816 27.598002896191897 29.40093633641172 21.562712136609566 46-47 21.594731859006068 27.73323701235819 29.047049939568826 21.624981189066915 48-49 21.636897411292814 27.781517351396655 28.796027121974653 21.785558115335874 50-51 21.6899664358481 27.833648673840628 28.635831060716615 21.840553829594658 52-53 21.70385576895988 27.833179003155827 28.597126034137304 21.86583919374699 54-55 21.73016246317509 28.153327257568023 28.483827745642504 21.632682533614382 56-57 21.605949736266368 27.866852891117734 28.56001605848527 21.967181314130624 58-59 21.522448009582998 27.535047791023963 28.888079660870865 22.05442453852217 60-61 21.684329093440084 27.81577101304218 28.68063367150264 21.819266222015095 62-63 21.496706403916622 27.875508241327303 28.647977711427213 21.97980764332886 64-65 21.565070215195387 27.654201115947753 28.766576434602197 22.01415223425466 66-67 21.480191185927033 27.663688289558646 28.67098010086399 22.185140423650328 68-69 21.770688922729686 27.79968330001586 28.5707906361012 21.858837141153252 70-71 21.761773194875644 27.670293923760198 28.57846751982701 21.989465361537146 72-73 21.599008346016603 27.80980424405257 28.529413945437458 22.061773464493367 74-75 21.649494264557 27.8699964006034 28.43895719953464 22.041552135304958 76-77 21.685452294145044 27.808677113012365 28.3943209032739 22.111549689568687 78-79 21.750381846099508 27.916640266598 28.4315258610579 21.901452026244588 80-81 21.685786269037806 27.77545699913604 28.437336701767062 22.101420030059092 82-83 21.572197247906626 27.869870156963252 28.516010008899084 22.041922586231042 84-85 21.583084049280725 27.823756961192576 28.32996423520911 22.263194754317592 86-87 21.686303374925476 27.84361543260243 28.328660494407593 22.141420698064504 88-89 21.485901478015133 27.78983571805315 28.41956352128554 22.30469928264617 90-91 21.454265787105214 27.70943803006275 28.48310699741386 22.35318918541818 92-93 21.56829967743316 27.863644314367686 28.307523380282067 22.26053262791708 94-95 21.405964265902025 27.60810248798674 28.452063908048764 22.53386933806247 96-97 21.41769040195716 27.654401165829785 28.376725599622805 22.551182832590257 98-99 21.380213564198 27.702833412645877 28.348079175940395 22.568873847215727 100 21.414960548186755 27.5818256085609 28.358661671548997 22.64455217170335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 69.0 1 65.5 2 196.5 3 342.5 4 222.5 5 145.5 6 448.0 7 1077.0 8 1880.0 9 2226.5 10 1734.0 11 1207.0 12 1212.0 13 1421.5 14 1667.5 15 1919.0 16 2152.5 17 2408.0 18 2724.5 19 3174.5 20 3806.0 21 4703.0 22 5964.0 23 7980.5 24 10838.5 25 14653.0 26 19671.0 27 25549.5 28 32880.5 29 41738.0 30 50359.0 31 58115.0 32 65338.0 33 73001.0 34 80083.0 35 85774.0 36 91137.5 37 96343.0 38 100770.5 39 102907.5 40 105148.0 41 106994.5 42 107741.5 43 108968.5 44 107624.0 45 105729.0 46 105334.0 47 102475.5 48 98499.0 49 95418.0 50 92683.0 51 89655.5 52 83890.5 53 78755.0 54 74845.0 55 69396.0 56 63215.5 57 57363.0 58 52072.0 59 47718.5 60 42581.5 61 35846.0 62 30457.5 63 25408.0 64 20201.5 65 15831.0 66 12110.0 67 9047.0 68 6795.0 69 5173.5 70 3666.0 71 2593.5 72 2028.5 73 1450.0 74 994.5 75 682.0 76 372.0 77 219.5 78 138.0 79 74.0 80 47.0 81 22.0 82 10.5 83 4.0 84 4.0 85 4.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.718310143962383E-4 2 3.7072436845418726E-4 3 6.740443062803404E-5 4 0.013244970618408687 5 4.044265837682043E-4 6 0.003639839253913838 7 8.762575981644425E-4 8 0.1411785799504173 9 0.0010447686747345278 10-11 0.0 12-13 1.0110664594205107E-4 14-15 1.516599689130766E-4 16-17 0.0 18-19 1.516599689130766E-4 20-21 0.0 22-23 2.3591550719811915E-4 24-25 4.886821220532468E-4 26-27 0.006319165371378192 28-29 0.008408702720847247 30-31 0.014980634707080566 32-33 0.01787902522408603 34-35 0.02625402572961926 36-37 0.03818460995078128 38-39 0.042869217879429655 40-41 0.05434482219385245 42-43 0.0716846119729142 44-45 0.1102736485074637 46-47 0.11667706941712692 48-49 0.11350906117760931 50-51 0.11581766292661948 52-53 0.11056011733763284 54-55 0.11718260264683719 56-57 0.10107294372673704 58-59 0.09259683657526177 60-61 0.09505709829318501 62-63 0.09352364749639723 64-65 0.0 66-67 0.0 68-69 8.425553828504256E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 6.740443062803404E-5 78-79 0.0 80-81 2.8646883016914465E-4 82-83 1.516599689130766E-4 84-85 0.0 86-87 5.729376603382893E-4 88-89 3.033199378261532E-4 90-91 1.1795775359905958E-4 92-93 9.099598134784595E-4 94-95 8.762575981644425E-4 96-97 2.3591550719811915E-4 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2967164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.16886413858274 #Duplication Level Percentage of deduplicated Percentage of total 1 72.58945515056975 42.224461545472366 2 15.726300510996316 18.295620756533378 3 5.367437095838587 9.366531576006713 4 2.3157007479306007 5.388067287679581 5 1.2214301132682186 3.552460115673637 6 0.7257715521107792 2.533038408622814 7 0.4470090486019973 1.8201406031792697 8 0.30540659012358196 1.4212123558341172 9 0.22470659451115943 1.1763834630446912 >10 1.0143963431986374 10.214269815099486 >50 0.0457987922644749 1.786014890814785 >100 0.015950049272260552 1.485136827869325 >500 4.0562538161723505E-4 0.1572085665611722 >1k 1.1589296604688547E-4 0.15666925744895294 >5k 1.1589296604688547E-4 0.42278453015968515 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6945 0.2340618853558482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5598 0.1886650013278673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2979 0.10039889942045671 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.370221531401702E-5 0.0 3 0.0 0.0 0.0 6.740443062803404E-5 0.0 4 0.0 0.0 0.0 6.740443062803404E-5 0.0 5 0.0 0.0 0.0 6.740443062803404E-5 0.0 6 0.0 0.0 0.0 6.740443062803404E-5 0.0 7 0.0 0.0 0.0 6.740443062803404E-5 0.0 8 0.0 0.0 0.0 6.740443062803404E-5 0.0 9 0.0 0.0 0.0 6.740443062803404E-5 0.0 10-11 0.0 0.0 0.0 1.0110664594205106E-4 0.0 12-13 0.0 0.0 0.0 1.0110664594205106E-4 0.0 14-15 0.0 0.0 0.0 1.3480886125606808E-4 0.0 16-17 0.0 0.0 0.0 2.5276661485512767E-4 0.0 18-19 0.0 1.685110765700851E-5 0.0 2.864688301691447E-4 0.0 20-21 0.0 3.370221531401702E-5 0.0 3.033199378261532E-4 0.0 22-23 0.0 3.370221531401702E-5 0.0 3.707243684541872E-4 0.0 24-25 0.0 3.370221531401702E-5 0.0 5.055332297102553E-4 0.0 26-27 0.0 5.055332297102553E-5 0.0 7.58299844565383E-4 0.0 28-29 0.0 6.740443062803404E-5 0.0 0.0014660463661597405 0.0 30-31 0.0 6.740443062803404E-5 0.0 0.003825201438140932 0.0 32-33 0.0 6.740443062803404E-5 0.0 0.00960513136449485 0.0 34-35 0.0 1.516599689130766E-4 0.0 0.01787902522408603 0.0 36-37 0.0 2.0221329188410214E-4 0.0 0.03355055534510394 0.0 38-39 0.0 2.3591550719811915E-4 0.0 0.06073139199585867 0.0 40-41 0.0 2.3591550719811915E-4 0.0 0.09751736001110825 0.0 42-43 0.0 2.3591550719811915E-4 0.0 0.13391575255024663 0.0 44-45 0.0 2.3591550719811915E-4 0.0 0.17592556393916886 0.0 46-47 0.0 2.6961772251213616E-4 0.0 0.22740569783132986 0.0 48-49 0.0 3.033199378261532E-4 0.0 0.28896279410238196 0.0 50-51 0.0 3.370221531401702E-4 0.0 0.3586926775870831 0.0 52-53 0.0 3.370221531401702E-4 0.0 0.42663634366014147 0.0 54-55 0.0 3.370221531401702E-4 0.0 0.5019102415639984 0.0 56-57 0.0 3.370221531401702E-4 0.0 0.5914064743303706 0.0 58-59 0.0 3.370221531401702E-4 0.0 0.696978663801529 0.0 60-61 0.0 3.370221531401702E-4 0.0 0.8126952200822064 0.0 62-63 0.0 3.370221531401702E-4 0.0 0.9318157001095997 0.0 64-65 0.0 3.370221531401702E-4 0.0 1.0518966932734424 0.0 66-67 0.0 3.370221531401702E-4 0.0 1.1906487137212505 0.0 68-69 0.0 3.370221531401702E-4 0.0 1.3529922848888702 0.0 70-71 0.0 3.707243684541872E-4 0.0 1.5310579395004793 0.0 72-73 0.0 4.0442658376820423E-4 0.0 1.7212058383021631 0.0 74-75 0.0 4.212776914252127E-4 0.0 1.9102752662137987 0.0 76-77 0.0 4.3812879908222127E-4 0.0 2.108225227860678 0.0 78-79 0.0 4.3812879908222127E-4 0.0 2.336625141043771 0.0 80-81 0.0 4.3812879908222127E-4 0.0 2.5942448749041174 0.0 82-83 0.0 4.3812879908222127E-4 0.0 2.8641322151387656 0.0 84-85 0.0 4.3812879908222127E-4 0.0 3.1279868588322044 0.0 86-87 0.0 4.718310143962383E-4 0.0 3.3976214324519978 0.0 88 0.0 4.718310143962383E-4 0.0 3.6206963956154765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4795 0.0 52.313747 1 GTATCAA 8980 0.0 42.580547 1 ATCAACG 10620 0.0 35.80057 3 TCAACGC 10615 0.0 35.773106 4 CAACGCA 10990 0.0 34.549538 5 AACGCAG 11170 0.0 33.908543 6 TATCAAC 11285 0.0 33.857136 2 ACGCAGA 12895 0.0 29.33453 7 CGCAGAG 13060 0.0 28.891867 8 GTACATG 9400 0.0 25.636145 1 GCAGAGT 14495 0.0 25.50209 9 GTGGTAT 2360 0.0 24.48253 1 TACATGG 9390 0.0 24.10323 2 ACATGGG 9375 0.0 23.48971 3 TGGTATC 2535 0.0 22.27404 2 CGCGCGT 195 4.6331843E-7 21.714624 7 GAGTACT 9515 0.0 21.22617 12-13 CCGATCG 135 4.0968478E-4 20.875492 9 CAGAGTA 14315 0.0 20.818634 10-11 AGAGTAC 13505 0.0 20.797855 10-11 >>END_MODULE