FastQCFastQC Report
Wed 25 May 2016
SRR1032968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4174245
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.034.6781431
GTATCAA59850.032.276561
TCAACGC61300.029.9029314
CAACGCA62900.029.291385
ATCAACG62800.029.2635293
AACGCAG66000.027.9155716
GTACATG132900.027.726861
TACATGG133400.026.601172
TATCAAC73150.025.6370562
ACATGGG135750.025.032723
ACGCAGA81950.022.1950427
CGCAGAG84900.021.0916848
CATGGGG85150.019.9265524
GTATAGG17000.018.524031
GAGTACA94200.018.161851
TATACTG27700.017.307125
GCAGAGT99600.017.1765549
TAATACG4551.0913936E-1116.527924
GTATTAG20950.016.3775331
GTACCGT3452.3910616E-816.3480726
Produced by FastQC (version 0.11.5)