FastQCFastQC Report
Wed 25 May 2016
SRR1032968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4174245
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89450.21429024889530918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.16920425130772151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.049.8264271
GTATCAA115100.042.03951
TCAACGC134900.035.1858754
ATCAACG135700.034.9437753
CAACGCA137350.034.5561685
AACGCAG141800.033.571246
TATCAAC144700.032.899212
ACGCAGA168700.028.0498397
CGCAGAG171950.027.3825668
GTACATG131650.026.4062371
TACATGG135050.024.6262532
GCAGAGT190150.024.3334839
ACATGGG135350.023.8426763
GAGTACT119900.021.17534612-13
AGAGTAC172850.020.1507210-11
GTGGTAT35600.020.0580731
CAGAGTA186100.019.9023410-11
CATGGGG84700.019.4945324
AGTACTT126950.019.11136212-13
GTACTTT133850.018.8280514-15
Produced by FastQC (version 0.11.5)