##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032968_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4174245
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82821612052	34.0	31.0	34.0	31.0	34.0
2	32.86889126057527	34.0	31.0	34.0	31.0	34.0
3	32.95260795664845	34.0	31.0	34.0	31.0	34.0
4	36.369283067956005	37.0	37.0	37.0	35.0	37.0
5	36.351497815772674	37.0	37.0	37.0	35.0	37.0
6	36.40791280818448	37.0	37.0	37.0	35.0	37.0
7	36.370568569885094	37.0	37.0	37.0	35.0	37.0
8	36.30452524947625	37.0	37.0	37.0	35.0	37.0
9	38.14329513480881	39.0	39.0	39.0	37.0	39.0
10-11	38.15820693322984	39.0	39.0	39.0	37.0	39.0
12-13	38.15210882447005	39.0	39.0	39.0	37.0	39.0
14-15	39.69432100894892	41.0	40.0	41.0	37.0	41.0
16-17	39.70672912107459	41.0	40.0	41.0	37.0	41.0
18-19	39.737860139977414	41.0	40.0	41.0	37.0	41.0
20-21	39.75314002891541	41.0	40.0	41.0	37.0	41.0
22-23	39.71689347414922	41.0	40.0	41.0	37.0	41.0
24-25	39.70057016298756	41.0	40.0	41.0	37.0	41.0
26-27	39.62873920912644	41.0	40.0	41.0	37.0	41.0
28-29	39.56871494126483	41.0	40.0	41.0	37.0	41.0
30-31	39.45917429379445	41.0	39.5	41.0	36.5	41.0
32-33	39.33694668137591	41.0	39.0	41.0	35.5	41.0
34-35	39.25055165664689	41.0	39.0	41.0	35.0	41.0
36-37	39.16299019343618	41.0	39.0	41.0	35.0	41.0
38-39	39.06958707502794	41.0	39.0	41.0	35.0	41.0
40-41	38.971801607236756	41.0	39.0	41.0	35.0	41.0
42-43	38.83647318257553	41.0	39.0	41.0	35.0	41.0
44-45	38.668726799696714	41.0	39.0	41.0	35.0	41.0
46-47	38.44780636977465	41.0	38.5	41.0	35.0	41.0
48-49	38.24393740664479	41.0	38.0	41.0	34.0	41.0
50-51	38.091780669318645	40.5	38.0	41.0	34.0	41.0
52-53	37.95126531863846	40.0	38.0	41.0	34.0	41.0
54-55	37.80628053695938	40.0	37.5	41.0	34.0	41.0
56-57	37.600462718407755	40.0	37.0	41.0	33.0	41.0
58-59	37.42915688944947	40.0	37.0	41.0	33.0	41.0
60-61	37.23957362349359	40.0	36.0	41.0	33.0	41.0
62-63	36.99979325602594	39.0	36.0	41.0	33.0	41.0
64-65	36.710035587273865	39.0	35.0	41.0	32.0	41.0
66-67	36.43275262951744	39.0	35.0	41.0	32.0	41.0
68-69	36.13574394890573	37.5	35.0	40.5	32.0	41.0
70-71	35.80309517050389	37.0	35.0	40.0	31.0	41.0
72-73	35.456857228073574	37.0	35.0	39.0	31.0	41.0
74-75	35.107403135177734	36.0	35.0	39.0	31.0	41.0
76-77	34.75387501212794	36.0	35.0	39.0	31.0	40.5
78-79	34.402824343084795	35.0	35.0	37.0	31.0	39.5
80-81	34.069617020563	35.0	34.0	37.0	30.0	39.0
82-83	33.76389382990217	35.0	34.0	36.5	30.0	39.0
84-85	33.48643048024253	35.0	34.0	36.0	30.0	37.0
86-87	33.34057308567178	35.0	34.0	36.0	30.0	37.0
88-89	33.150971013919886	35.0	34.0	35.5	30.0	37.0
90-91	32.9807147160744	35.0	34.0	35.0	30.0	36.0
92-93	32.82298846857336	35.0	34.0	35.0	30.0	36.0
94-95	32.72358091103901	35.0	34.0	35.0	29.5	36.0
96-97	32.660960365287615	35.0	34.0	35.0	30.0	36.0
98-99	32.5511371517484	35.0	34.0	35.0	30.0	36.0
100	32.46862582335249	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19084096119961913
1101	2	0.2230523907121622
1101	3	0.2050571445379319
1101	4	0.14225256454469815
1101	5	0.1419258605821767
1101	6	0.14373605078893803
1101	7	0.11010256352103909
1101	8	-0.021967162039111088
1101	9	0.10826474465012126
1101	10-11	0.16707869662538855
1101	12-13	0.21444429036297663
1101	14-15	0.30895226471326964
1101	16-17	0.2665153479620912
1101	18-19	0.2627348100703273
1101	20-21	0.26760764733916886
1101	22-23	0.24755130690421367
1101	24-25	0.2723126670775784
1101	26-27	0.2711498340468168
1101	28-29	0.21519493356224473
1101	30-31	0.2677363245696185
1101	32-33	0.3320420426895083
1101	34-35	0.3027839417137983
1101	36-37	0.297552992785576
1101	38-39	0.3133287306696815
1101	40-41	0.3677887155992323
1101	42-43	0.34247802138860095
1101	44-45	0.3574399883084993
1101	46-47	0.4102199977092198
1101	48-49	0.38339670089033007
1101	50-51	0.382102355830213
1101	52-53	0.41540771545130184
1101	54-55	0.42092189432512583
1101	56-57	0.4508880346470079
1101	58-59	0.4523464758445286
1101	60-61	0.4222314362182118
1101	62-63	0.36051752589047226
1101	64-65	0.36762054449120285
1101	66-67	0.37751853396672175
1101	68-69	0.3630971732173194
1101	70-71	0.4025338049523626
1101	72-73	0.4168785630640244
1101	74-75	0.43038119038611455
1101	76-77	0.43655588269326984
1101	78-79	0.4414000468070185
1101	80-81	0.4726566293351553
1101	82-83	0.4357245053098424
1101	84-85	0.48056851627278263
1101	86-87	0.4837009307957203
1101	88-89	0.47245695366703444
1101	90-91	0.43072913791247913
1101	92-93	0.45040273280341836
1101	94-95	0.45191941206581987
1101	96-97	0.45857518319046164
1101	98-99	0.47959501168413965
1101	100	0.40888355422432454
1102	1	0.15321470430138362
1102	2	0.16502826686674865
1102	3	0.17850019271757844
1102	4	0.0941729287770059
1102	5	0.08403021467896821
1102	6	0.05976381289339372
1102	7	0.06876231943303424
1102	8	-0.011679416342033733
1102	9	0.03968747216772073
1102	10-11	0.09918785077623227
1102	12-13	0.10377560609051528
1102	14-15	0.17387983032621435
1102	16-17	0.1586176523188314
1102	18-19	0.1611926376435946
1102	20-21	0.18781247839333304
1102	22-23	0.16338195135666922
1102	24-25	0.12475078855639055
1102	26-27	0.12754876804022786
1102	28-29	0.14626574965885197
1102	30-31	0.19563506457610202
1102	32-33	0.2045258243429231
1102	34-35	0.18633859380852158
1102	36-37	0.23504738110533907
1102	38-39	0.1647548594562096
1102	40-41	0.1257031340495871
1102	42-43	0.15796928323456427
1102	44-45	0.17904750904690303
1102	46-47	0.15081671188733026
1102	48-49	0.15511473038012014
1102	50-51	0.2007074799676687
1102	52-53	0.16274284664883965
1102	54-55	0.17702450797405334
1102	56-57	0.15030798184287164
1102	58-59	0.1673165071092697
1102	60-61	0.16805918149944432
1102	62-63	0.21093065778929088
1102	64-65	0.26239459934248544
1102	66-67	0.24245227242792566
1102	68-69	0.17473625286395134
1102	70-71	0.17810507990721192
1102	72-73	0.17982928731338887
1102	74-75	0.13480652006937532
1102	76-77	0.15046740339008835
1102	78-79	0.14937467563275675
1102	80-81	0.1393649405761863
1102	82-83	0.17795711288162153
1102	84-85	0.15800612383983292
1102	86-87	0.16911244062360709
1102	88-89	0.11618691110805912
1102	90-91	0.13067767585121715
1102	92-93	0.1616926537058987
1102	94-95	0.17087795982440213
1102	96-97	0.1839047010537982
1102	98-99	0.21446205266759932
1102	100	0.18308814876093038
1103	1	0.18479829748293497
1103	2	0.19900789096140414
1103	3	0.17645871649546052
1103	4	0.059683121887672996
1103	5	0.0838005693792283
1103	6	0.1141784586584933
1103	7	0.1453688278570695
1103	8	0.04633502122356248
1103	9	0.12336991564414035
1103	10-11	0.14309464802494176
1103	12-13	0.17220364418263756
1103	14-15	0.2371455343509652
1103	16-17	0.21811875234676137
1103	18-19	0.22412946231906972
1103	20-21	0.199806889077756
1103	22-23	0.2175335681466919
1103	24-25	0.17859443019556664
1103	26-27	0.2132643776393266
1103	28-29	0.23602864991336503
1103	30-31	0.20777040308484374
1103	32-33	0.2523371791252629
1103	34-35	0.28646734017328157
1103	36-37	0.2822887490325314
1103	38-39	0.2814125368877214
1103	40-41	0.25698657217040477
1103	42-43	0.26082011729344856
1103	44-45	0.2764954603151111
1103	46-47	0.33570828629469673
1103	48-49	0.38415310630471566
1103	50-51	0.32330768085428474
1103	52-53	0.37131475147493376
1103	54-55	0.35664017217909816
1103	56-57	0.3940365073996901
1103	58-59	0.33535266383614015
1103	60-61	0.33864981981136566
1103	62-63	0.35932583717510624
1103	64-65	0.37580767008584814
1103	66-67	0.4244711744081826
1103	68-69	0.43692067827043957
1103	70-71	0.4517535167373907
1103	72-73	0.4090165429678052
1103	74-75	0.39514985727235086
1103	76-77	0.40759864358703624
1103	78-79	0.3964878187255749
1103	80-81	0.3587399603484869
1103	82-83	0.331861243448067
1103	84-85	0.3039221569903674
1103	86-87	0.37782457121389257
1103	88-89	0.35625614168683484
1103	90-91	0.34749630844670065
1103	92-93	0.4152724726122159
1103	94-95	0.39708838025354254
1103	96-97	0.35315844495418247
1103	98-99	0.3706229053594541
1103	100	0.3807688229312163
1104	1	0.12473186459239827
1104	2	0.16524174433223493
1104	3	0.17177848434158705
1104	4	0.10013106409088124
1104	5	0.0778999852380764
1104	6	0.10476981964571763
1104	7	0.08007825127815238
1104	8	-0.017996992305690185
1104	9	0.03706860217285879
1104	10-11	0.13306462734388447
1104	12-13	0.11808597013828859
1104	14-15	0.18040751925629905
1104	16-17	0.17351893455185774
1104	18-19	0.20328448353338757
1104	20-21	0.1693468458540437
1104	22-23	0.16844569066989834
1104	24-25	0.17771386362779396
1104	26-27	0.21129614036646416
1104	28-29	0.232760263506691
1104	30-31	0.24003767645914564
1104	32-33	0.21309122212988996
1104	34-35	0.2505006843826365
1104	36-37	0.2544538116948232
1104	38-39	0.23550065786977825
1104	40-41	0.2858835138162945
1104	42-43	0.28884224064718467
1104	44-45	0.28631816908984575
1104	46-47	0.2795384085997199
1104	48-49	0.29130561501010277
1104	50-51	0.2746797208317062
1104	52-53	0.3380262103673388
1104	54-55	0.35054310782206954
1104	56-57	0.350910602877363
1104	58-59	0.37364051487363525
1104	60-61	0.4147065703341539
1104	62-63	0.3275309335436276
1104	64-65	0.3603468586708942
1104	66-67	0.3175139380882257
1104	68-69	0.3310878046606405
1104	70-71	0.3036381006711011
1104	72-73	0.3541838249908409
1104	74-75	0.2673630447751165
1104	76-77	0.2758667133537571
1104	78-79	0.31708875758657484
1104	80-81	0.32193168775157943
1104	82-83	0.3286194930269417
1104	84-85	0.32222177163150434
1104	86-87	0.2891379726854879
1104	88-89	0.293992219210935
1104	90-91	0.3282868179577818
1104	92-93	0.3151834560626696
1104	94-95	0.33020852320304783
1104	96-97	0.35442659766228957
1104	98-99	0.3813924867148941
1104	100	0.38656424825679636
1105	1	0.04588068936699585
1105	2	0.09054652532267227
1105	3	0.0885985525318631
1105	4	0.06125808511166042
1105	5	0.0489705157269853
1105	6	0.04680392411383849
1105	7	0.05193930568007943
1105	8	0.012419928145227743
1105	9	0.06588382569712081
1105	10-11	0.0731833620581881
1105	12-13	0.055246065133694344
1105	14-15	0.13049351388053054
1105	16-17	0.11251135227632147
1105	18-19	0.12517719282103457
1105	20-21	0.12303613504627009
1105	22-23	0.16207014168612233
1105	24-25	0.1387378909637036
1105	26-27	0.12196863465641883
1105	28-29	0.12250452320249394
1105	30-31	0.16380101136471836
1105	32-33	0.18698977816134743
1105	34-35	0.22733601170162387
1105	36-37	0.24317493628672793
1105	38-39	0.2519525836091461
1105	40-41	0.2297047197481561
1105	42-43	0.23895397375244443
1105	44-45	0.14277643640923543
1105	46-47	0.26337893668230805
1105	48-49	0.29015081604513426
1105	50-51	0.3028021391971123
1105	52-53	0.263364006062325
1105	54-55	0.2988892765550659
1105	56-57	0.3191684422328649
1105	58-59	0.3232621830590787
1105	60-61	0.3426529518032524
1105	62-63	0.3630298521298272
1105	64-65	0.36221002251495804
1105	66-67	0.34942467120445997
1105	68-69	0.36080647272526534
1105	70-71	0.27295658545441626
1105	72-73	0.3134615586812757
1105	74-75	0.3222879162430772
1105	76-77	0.28854681181418584
1105	78-79	0.2707396908755655
1105	80-81	0.29628282753687074
1105	82-83	0.3181561510651747
1105	84-85	0.25737361568101846
1105	86-87	0.2880904218093079
1105	88-89	0.2930425063712718
1105	90-91	0.2917016479987282
1105	92-93	0.2850957681591666
1105	94-95	0.28964029017176784
1105	96-97	0.3171159391797076
1105	98-99	0.3561522348266166
1105	100	0.3212204211159033
1106	1	0.09204693535579622
1106	2	0.09352437454737128
1106	3	0.07178523198061271
1106	4	0.06582493400117784
1106	5	0.05638702524845485
1106	6	0.0683324649239978
1106	7	0.03506286946835502
1106	8	0.04861728052079428
1106	9	0.09605227257450366
1106	10-11	0.0728953634946734
1106	12-13	0.087507572709832
1106	14-15	0.10413437545226145
1106	16-17	0.1013960566141563
1106	18-19	0.12492180698099986
1106	20-21	0.11745586420357057
1106	22-23	0.11055671813154078
1106	24-25	0.14381992174960345
1106	26-27	0.1763752109472847
1106	28-29	0.18496688702658304
1106	30-31	0.21153313833399778
1106	32-33	0.19677425610129262
1106	34-35	0.13020423845456008
1106	36-37	0.1917810334069614
1106	38-39	0.1939701353041272
1106	40-41	0.19889625770471753
1106	42-43	0.24279994927373139
1106	44-45	0.25256762753723194
1106	46-47	0.2678366613147034
1106	48-49	0.22121425468087352
1106	50-51	0.25325354961987756
1106	52-53	0.24605643625520912
1106	54-55	0.2545362649467151
1106	56-57	0.27131739193794147
1106	58-59	0.24524417290495393
1106	60-61	0.21899358765832488
1106	62-63	0.24271441058226628
1106	64-65	0.2592913210270211
1106	66-67	0.24295728344498002
1106	68-69	0.23692213671039752
1106	70-71	0.30360197117091303
1106	72-73	0.283761242919951
1106	74-75	0.28290364242878496
1106	76-77	0.25508278949457974
1106	78-79	0.221456387883336
1106	80-81	0.2688606490445622
1106	82-83	0.2849992297733692
1106	84-85	0.2804132338615233
1106	86-87	0.2963506425682212
1106	88-89	0.2752656234592905
1106	90-91	0.28879605260605246
1106	92-93	0.2365902265126678
1106	94-95	0.24507013578064374
1106	96-97	0.20407363064015982
1106	98-99	0.20053218561216113
1106	100	0.20684348198700775
1107	1	0.05831096462299712
1107	2	0.049449421212337086
1107	3	0.04370581708153054
1107	4	0.02513967924883076
1107	5	0.04044099221432873
1107	6	0.025635134186643427
1107	7	0.02798090116886698
1107	8	0.015895974464385176
1107	9	0.0390995917981769
1107	10-11	0.02428375474262623
1107	12-13	0.01285852434580903
1107	14-15	0.03970468797446358
1107	16-17	0.022117027798380207
1107	18-19	0.041422124060652266
1107	20-21	0.050953542400229423
1107	22-23	0.03877854193602559
1107	24-25	0.020166162333900672
1107	26-27	0.08031578701567099
1107	28-29	0.07031659171460802
1107	30-31	0.08334507623325038
1107	32-33	0.10580406100001483
1107	34-35	0.13404232853812204
1107	36-37	0.12276947394465054
1107	38-39	0.07322425457018511
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2216	28-29	-0.3553895052936582
2216	30-31	-0.35004272741976905
2216	32-33	-0.4692138565098247
2216	34-35	-0.46024336167654667
2216	36-37	-0.3982142364307606
2216	38-39	-0.4236146394050593
2216	40-41	-0.28344576644920494
2216	42-43	-0.3374656632351787
2216	44-45	-0.3477316460273343
2216	46-47	-0.3366507732112751
2216	48-49	-0.3276875492220981
2216	50-51	-0.330681849902561
2216	52-53	-0.30543201528123376
2216	54-55	-0.3205809265714379
2216	56-57	-0.35449106999666924
2216	58-59	-0.37274898114858246
2216	60-61	-0.4146976112685721
2216	62-63	-0.4018418700573676
2216	64-65	-0.42545339195302745
2216	66-67	-0.45726869306646023
2216	68-69	-0.4609961592000431
2216	70-71	-0.45532174468871034
2216	72-73	-0.4132100613732703
2216	74-75	-0.42193958163719714
2216	76-77	-0.4679854032377122
2216	78-79	-0.4652059676684246
2216	80-81	-0.4672952709329934
2216	82-83	-0.43260978403341
2216	84-85	-0.4389800882969297
2216	86-87	-0.3932914999378099
2216	88-89	-0.3716381643324027
2216	90-91	-0.37139581012511513
2216	92-93	-0.3702220256477844
2216	94-95	-0.4382666228279106
2216	96-97	-0.43424367210672443
2216	98-99	-0.46403552483199917
2216	100	-0.4811104708893055
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19.0
3	10.0
4	15.0
5	36.0
6	82.0
7	225.0
8	491.0
9	940.0
10	1629.0
11	2603.0
12	4201.0
13	6343.0
14	7785.0
15	9915.0
16	12942.0
17	18859.0
18	22791.0
19	12664.0
20	8621.0
21	8064.0
22	8212.0
23	8520.0
24	9843.0
25	11688.0
26	14723.0
27	18760.0
28	24061.0
29	31115.0
30	39546.0
31	49780.0
32	64238.0
33	83409.0
34	117408.0
35	182244.0
36	344687.0
37	807447.0
38	1497034.0
39	741046.0
40	2249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.317155510964525	19.632708571850205	13.52277172836303	23.527364188822233
2	15.655665108773434	20.052934351677568	40.31563209188192	23.975768447667075
3	18.303671639309584	25.551603537794556	30.064090724081684	26.08063409881418
4	12.326933089140285	16.6483977107381	38.882208241179924	32.14246095894169
5	12.501844650704609	38.01702924034888	35.78152819488711	13.6995979140594
6	30.79677429324167	38.25065708885739	17.482049393724576	13.470519224176366
7	27.358165343421952	32.729075137349376	22.268290830669955	17.644468688558717
8	25.451026661433477	36.085323688400074	19.989357690052557	18.474291960113888
9	25.387273111452146	16.339161695238307	21.051361870314356	37.22220332299519
10-11	24.007754695759353	26.633774490955847	28.682695912674028	20.67577490061077
12-13	25.03107752095651	24.0097057212105	29.008640856453116	21.950575901379867
14-15	22.027329768692	25.843845327993282	26.516662496246322	25.6121624070684
16-17	21.213141538170376	29.401712165912635	27.015017086922306	22.37012920899468
18-19	21.068161038675427	27.867962535451067	29.808735219912514	21.25514120596099
20-21	22.14267490288663	27.17345292382215	29.547762529511324	21.136109643779893
22-23	21.810130695819797	26.93086600668937	29.640584399367114	21.618418898123714
24-25	21.572090815910837	27.06182908945123	29.624915957443115	21.741164137194822
26-27	21.32635406920032	27.120866673287043	29.679992745539124	21.872786511973512
28-29	21.452534043298705	27.184434268806545	29.746682519099288	21.616349168795466
30-31	21.6386839522643	27.506404469183792	29.34194498433984	21.512966594212067
32-33	21.401029424855018	27.218083738199788	29.72072211411274	21.660164722832455
34-35	21.57102089807745	27.00213435433445	29.7347285353853	21.692116212202794
36-37	21.819333467699707	27.43868375915571	29.144581492286353	21.597401280858232
38-39	21.692405292036494	27.568474026580976	29.23152252221592	21.507598159166612
40-41	21.549658219674217	27.260882008486142	29.443012403229357	21.746447368610287
42-43	21.53734566813552	27.221682420797226	29.284427084494546	21.95654482657271
44-45	21.71563875563776	27.419313449029588	29.117475934454035	21.747571860878626
46-47	21.807708174435284	27.59024210066821	28.76545208594166	21.836597638954846
48-49	21.83709200449937	27.544615982367066	28.586726850175204	22.031565162958362
50-51	21.855074278233918	27.60435516841668	28.419857960071386	22.12071259327801
52-53	21.91664587152355	27.584792320974206	28.401708683248337	22.096853124253908
54-55	21.91476251887878	27.9340033477536	28.25328473438269	21.897949398984927
56-57	21.81190784455117	27.615675721173965	28.412790185995775	22.159626248279093
58-59	21.688714110583913	27.24755941479907	28.70564609191647	22.358080382700546
60-61	21.911648706908178	27.628365903567264	28.36392529018627	22.096060099338285
62-63	21.694075957914443	27.61821395840457	28.422556602999933	22.265153480681054
64-65	21.7582461019897	27.32818749258848	28.55482847796428	22.35873792745754
66-67	21.625010810343337	27.45734975179931	28.421201161504012	22.49643827635334
68-69	21.973988079267123	27.578457395837326	28.306494116294225	22.141060408601327
70-71	21.925141184231062	27.41781177408271	28.319855245661817	22.337191796024406
72-73	21.72318586954048	27.538848342634413	28.35219303131464	22.38577275651046
74-75	21.82575531623084	27.65373139334179	28.13460877356264	22.385904516864727
76-77	21.89182416635743	27.630436287698767	28.041564921622875	22.436174624320923
78-79	21.945034371485143	27.685246074439807	28.109370676613377	22.260348877461674
80-81	21.835505847959872	27.52893435405566	28.218438187513943	22.417121610470524
82-83	21.73158050264737	27.60322631301494	28.255848342158696	22.409344842179
84-85	21.745273696201348	27.60418950013715	28.064907546155055	22.585629257506447
86-87	21.859224221438282	27.66532761394076	28.043601923665012	22.431846240955945
88-89	21.565958647692785	27.606583155835484	28.084994857733008	22.742463338738723
90-91	21.565044550635456	27.53476741509051	28.122155918719553	22.778032115554474
92-93	21.69499183436934	27.676780453657862	27.943945227388316	22.684282484584482
94-95	21.45584525707378	27.451747945504774	28.09490104751796	22.997505749903485
96-97	21.479924752634165	27.474439912007774	28.10039929496021	22.945236040397845
98-99	21.458961016778005	27.562388834674156	27.954615499882674	23.024034648665165
100	21.53939215355112	27.37239908055229	27.88552181292665	23.202686952969938
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	60.0
2	232.0
3	411.0
4	286.0
5	199.0
6	571.5
7	1399.0
8	2373.0
9	2735.0
10	2179.5
11	1631.5
12	1680.5
13	1951.5
14	2212.5
15	2428.5
16	2677.5
17	3058.5
18	3519.5
19	3983.5
20	4626.0
21	5560.5
22	7111.5
23	9549.0
24	12775.0
25	17228.5
26	23377.5
27	30875.5
28	40361.0
29	52313.0
30	64021.5
31	74687.5
32	84780.5
33	95800.0
34	108005.0
35	118400.5
36	127305.5
37	135504.5
38	141931.0
39	145111.0
40	148733.0
41	152775.0
42	153518.0
43	153842.5
44	152186.0
45	149506.5
46	149080.5
47	146264.5
48	141722.0
49	137684.0
50	133422.0
51	128379.0
52	119845.0
53	112249.0
54	108008.0
55	101919.5
56	93431.0
57	84935.0
58	76855.0
59	70949.5
60	63365.0
61	53565.5
62	45959.5
63	38505.0
64	30695.0
65	23942.0
66	18733.0
67	14211.0
68	10579.0
69	7897.5
70	5573.0
71	3933.5
72	2968.0
73	2154.0
74	1469.5
75	972.0
76	612.5
77	379.5
78	226.5
79	123.0
80	69.0
81	34.5
82	14.5
83	7.0
84	3.5
85	2.5
86	3.0
87	2.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.8747713658398106E-4
2	4.312157048759716E-4
3	1.4373856829199053E-4
4	0.011930301168235215
5	3.114335646326461E-4
6	0.0036413770633970935
7	8.624314097519432E-4
8	0.14318757044687122
9	0.0014134292548712401
10-11	0.0
12-13	1.4373856829199053E-4
14-15	1.31760354267658E-4
16-17	0.0
18-19	1.31760354267658E-4
20-21	0.0
22-23	2.5154249451098343E-4
24-25	4.911067749976343E-4
26-27	0.00626460593472592
28-29	0.00796551232618114
30-31	0.014301987545053057
32-33	0.017955342822474483
34-35	0.026016680860850284
36-37	0.03770741774859885
38-39	0.042810136922964516
40-41	0.054309222386323755
42-43	0.07086311416795134
44-45	0.10998396117142142
46-47	0.11750627957870226
48-49	0.11439194393237578
50-51	0.11628450174822035
52-53	0.11023550366593239
54-55	0.11695528173358295
56-57	0.10071282351658804
58-59	0.0930108318989422
60-61	0.09461591257820276
62-63	0.09302281011296654
64-65	0.0
66-67	4.791285609733018E-5
68-69	1.1978214024332543E-4
70-71	1.1978214024332544E-5
72-73	0.0
74-75	0.0
76-77	9.582571219466035E-5
78-79	0.0
80-81	2.7549892255964846E-4
82-83	1.5571678231632305E-4
84-85	0.0
86-87	6.108889152409598E-4
88-89	3.833028487786414E-4
90-91	1.4373856829199053E-4
92-93	0.0010061699780439337
94-95	0.0010660610481655964
96-97	2.994553506083136E-4
98-99	1.1978214024332544E-5
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4174245.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.407225288213255
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.09554101782034	38.681111171439575
2	15.933067050765127	17.33747937126372
3	5.625301802948002	9.181711875215525
4	2.588096177804225	5.632445272534324
5	1.3832325273461261	3.7628921870652645
6	0.8233413931157929	2.687743239861738
7	0.5514019961572348	2.1000176840508025
8	0.37715471945339096	1.6415953431850805
9	0.26225839398478845	1.284187637272984
>10	1.2639992389426076	12.083703252513196
>50	0.07134011405236611	2.6514202842626937
>100	0.02438364666545	2.155458415065394
>500	6.614407387239529E-4	0.24478462166923515
>1k	1.322881235642124E-4	0.1713947935826595
>5k	8.819208237613988E-5	0.3840548510178034
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8945	0.21429024889530918	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7063	0.16920425130772151	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.395642804866509E-5	0.0	0.0	0.0
6	0.0	2.395642804866509E-5	0.0	0.0	0.0
7	0.0	2.395642804866509E-5	0.0	0.0	0.0
8	0.0	2.395642804866509E-5	0.0	0.0	0.0
9	0.0	2.395642804866509E-5	0.0	0.0	0.0
10-11	0.0	2.395642804866509E-5	0.0	2.395642804866509E-5	0.0
12-13	0.0	3.593464207299763E-5	0.0	2.395642804866509E-5	0.0
14-15	0.0	5.989107012166272E-5	0.0	2.395642804866509E-5	0.0
16-17	0.0	7.186928414599527E-5	0.0	2.395642804866509E-5	0.0
18-19	0.0	7.186928414599527E-5	0.0	4.791285609733018E-5	0.0
20-21	0.0	7.186928414599527E-5	0.0	5.989107012166272E-5	0.0
22-23	0.0	7.186928414599527E-5	0.0	9.582571219466035E-5	0.0
24-25	0.0	7.186928414599527E-5	0.0	1.6769499634065562E-4	0.0
26-27	0.0	7.186928414599527E-5	0.0	3.473682067056438E-4	0.0
28-29	0.0	7.186928414599527E-5	0.0	0.0012816689006035823	0.0
30-31	0.0	7.186928414599527E-5	0.0	0.005270414170706319	0.0
32-33	0.0	7.186928414599527E-5	0.0	0.014385835043223386	0.0
34-35	0.0	9.582571219466035E-5	0.0	0.026867134056577896	0.0
36-37	0.0	1.4373856829199053E-4	0.0	0.05130269066621629	0.0
38-39	0.0	1.7967321036498816E-4	0.0	0.09242389941174992	0.0
40-41	0.0	1.916514243893207E-4	0.0	0.1425048122474843	0.0
42-43	0.0	2.63520708535316E-4	0.0	0.19306485364419196	0.0
44-45	0.0	2.63520708535316E-4	0.0	0.2508717145256208	0.0
46-47	0.0	2.63520708535316E-4	0.0	0.3236872775795383	0.0
48-49	0.0	2.63520708535316E-4	0.0	0.40907996535900504	0.0
50-51	0.0	2.63520708535316E-4	0.0	0.5009169322835627	0.0
52-53	0.0	2.63520708535316E-4	0.0	0.5903582564032538	0.0
54-55	0.0	2.63520708535316E-4	0.0	0.6902805177942358	0.0
56-57	0.0	2.63520708535316E-4	0.0	0.8093559434101256	0.0
58-59	0.0	2.63520708535316E-4	0.0	0.9456201061509327	0.0
60-61	0.0	2.63520708535316E-4	0.0	1.0897419772917019	0.0
62-63	0.0	2.63520708535316E-4	0.0	1.2384874390458633	0.0
64-65	0.0	2.63520708535316E-4	0.0	1.3965759077390043	0.0
66-67	0.0	3.234117786569787E-4	0.0	1.5759496627533842	0.0
68-69	0.0	3.3538999268131124E-4	0.0	1.7784773054767988	0.0
70-71	2.395642804866509E-5	3.3538999268131124E-4	0.0	2.00170330203426	0.0
72-73	2.395642804866509E-5	3.3538999268131124E-4	0.0	2.237314771892881	0.0
74-75	2.395642804866509E-5	3.3538999268131124E-4	0.0	2.472159636053945	0.0
76-77	2.395642804866509E-5	4.312157048759716E-4	0.0	2.7232469584320036	0.0
78-79	2.395642804866509E-5	4.312157048759716E-4	0.0	3.011442787857445	0.0
80-81	2.395642804866509E-5	4.312157048759716E-4	0.0	3.332111555502851	0.0
82-83	2.395642804866509E-5	4.312157048759716E-4	0.0	3.657823151252502	0.0
84-85	2.395642804866509E-5	4.312157048759716E-4	0.0	3.9842534398436125	0.0
86-87	2.395642804866509E-5	4.312157048759716E-4	0.0	4.321260491418208	0.0
88	2.395642804866509E-5	4.312157048759716E-4	0.0	4.602197523144904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6185	0.0	49.826427	1
GTATCAA	11510	0.0	42.0395	1
TCAACGC	13490	0.0	35.185875	4
ATCAACG	13570	0.0	34.943775	3
CAACGCA	13735	0.0	34.556168	5
AACGCAG	14180	0.0	33.57124	6
TATCAAC	14470	0.0	32.89921	2
ACGCAGA	16870	0.0	28.049839	7
CGCAGAG	17195	0.0	27.382566	8
GTACATG	13165	0.0	26.406237	1
TACATGG	13505	0.0	24.626253	2
GCAGAGT	19015	0.0	24.333483	9
ACATGGG	13535	0.0	23.842676	3
GAGTACT	11990	0.0	21.175346	12-13
AGAGTAC	17285	0.0	20.15072	10-11
GTGGTAT	3560	0.0	20.058073	1
CAGAGTA	18610	0.0	19.90234	10-11
CATGGGG	8470	0.0	19.494532	4
AGTACTT	12695	0.0	19.111362	12-13
GTACTTT	13385	0.0	18.82805	14-15
>>END_MODULE