FastQCFastQC Report
Wed 25 May 2016
SRR1032967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589813
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94250.033.063691
GTACATG217900.030.824371
TACATGG217450.029.1589372
GGTATCA61650.028.8951721
TCAACGC105100.028.890414
CAACGCA106350.028.5060255
ATCAACG107150.028.4692753
AACGCAG108000.028.1572486
ACATGGG230150.026.73333
TATCAAC122700.025.2441082
TATACTG13700.024.0155165
ACGCAGA129100.023.336817
CATGGGG160700.022.4045814
GAGTACA148300.022.344321
CGCAGAG137150.021.8642488
AGTACAT147000.021.5826912
CATGGGA58750.020.6412374
ACACCGT10750.019.6749556
GCAGAGT147700.019.6338259
GTACACA20600.018.9378221
Produced by FastQC (version 0.11.5)