FastQCFastQC Report
Wed 25 May 2016
SRR1032967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589813
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87270.19013846533616946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72870.15876463812360112No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90500.042.834541
GTATCAA150500.037.21591
TCAACGC179750.030.5694964
CAACGCA179950.030.532195
GTACATG222250.030.296551
ATCAACG182800.030.0851843
TACATGG216100.029.8236772
AACGCAG184950.029.7576396
TATCAAC193050.028.8277232
ACATGGG235650.026.5720423
ACGCAGA215900.025.2079897
CGCAGAG225050.024.035478
CATGGGG162300.022.5225144
GCAGAGT238850.022.5220289
GAGTACA154100.022.411631
AGTACAT150700.021.227122
CATGGGA63000.020.6849064
GTACGGT2351.2709279E-719.9949761
TACCTGG29050.019.2706432
GTACACA23700.019.2314571
Produced by FastQC (version 0.11.5)