FastQCFastQC Report
Wed 25 May 2016
SRR1032966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278724
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG113500.028.7391321
TACATGG118050.026.3175542
GTATCAA64750.026.2045971
ACATGGG115950.025.3349063
TCAACGC65450.025.206214
ATCAACG67350.024.5649073
CAACGCA67300.024.512575
AACGCAG69200.023.7716186
GGTATCA45400.023.1900771
TATCAAC76300.021.9914422
CATGGGG77950.021.7671284
ACGCAGA81700.019.846957
ACCGCGT2151.1954035E-619.6743728
GAGTACA81500.019.3195611
CGCAGAG85300.019.0644348
TACCTGG21450.018.8443532
AGTACAT84800.017.9026242
GTATAGA13550.017.6905331
GCGTTAG1600.00127903817.6254961
CATGGGA37350.017.114274
Produced by FastQC (version 0.11.5)