##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032966_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3278724
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704147711121767	33.0	31.0	34.0	30.0	34.0
2	31.829910660366654	34.0	31.0	34.0	30.0	34.0
3	31.99771618471088	34.0	31.0	34.0	30.0	34.0
4	35.32337610607053	37.0	35.0	37.0	33.0	37.0
5	35.41673529092415	37.0	35.0	37.0	35.0	37.0
6	35.46664220593133	37.0	37.0	37.0	35.0	37.0
7	35.39354273186764	37.0	37.0	37.0	35.0	37.0
8	35.41740780864751	37.0	37.0	37.0	35.0	37.0
9	37.09641189682328	39.0	38.0	39.0	35.0	39.0
10-11	37.08959766055331	39.0	38.0	39.0	35.0	39.0
12-13	37.05161184655982	39.0	38.0	39.0	35.0	39.0
14-15	38.55594401968571	41.0	39.0	41.0	35.0	41.0
16-17	38.58246973517747	41.0	39.0	41.0	35.0	41.0
18-19	38.58825750505379	41.0	39.5	41.0	35.0	41.0
20-21	38.62543492529411	41.0	39.5	41.0	35.0	41.0
22-23	38.62156665214883	41.0	39.0	41.0	35.0	41.0
24-25	38.649564739209524	41.0	39.0	41.0	35.0	41.0
26-27	38.62506115183834	41.0	39.0	41.0	35.0	41.0
28-29	38.6093454343824	41.0	39.0	41.0	35.0	41.0
30-31	38.56539403743652	41.0	39.0	41.0	34.5	41.0
32-33	38.51656223579661	41.0	39.0	41.0	34.0	41.0
34-35	38.45481458640617	41.0	39.0	41.0	34.0	41.0
36-37	38.37469957825056	41.0	39.0	41.0	34.0	41.0
38-39	38.29162610210558	41.0	39.0	41.0	34.0	41.0
40-41	38.207944462540915	41.0	39.0	41.0	33.5	41.0
42-43	38.10118814514427	41.0	38.0	41.0	33.0	41.0
44-45	37.97632676614439	40.0	38.0	41.0	33.0	41.0
46-47	37.85788282270786	40.0	38.0	41.0	33.0	41.0
48-49	37.73021776154382	40.0	38.0	41.0	33.0	41.0
50-51	37.59047650854418	40.0	37.5	41.0	33.0	41.0
52-53	37.434057425998645	40.0	37.0	41.0	32.0	41.0
54-55	37.26435726215443	40.0	37.0	41.0	32.0	41.0
56-57	37.07913810372571	40.0	36.0	41.0	31.5	41.0
58-59	36.867339702884415	40.0	36.0	41.0	31.0	41.0
60-61	36.61868199336083	39.0	35.0	41.0	31.0	41.0
62-63	36.36702799625708	39.0	35.0	41.0	31.0	41.0
64-65	36.08992293953379	38.5	35.0	41.0	30.5	41.0
66-67	35.79048709192966	38.0	35.0	40.0	30.0	41.0
68-69	35.47386452778581	37.0	35.0	40.0	30.0	41.0
70-71	35.13446008264191	37.0	35.0	39.5	30.0	41.0
72-73	34.8384739002124	36.0	34.5	39.0	29.5	41.0
74-75	34.55717163140295	36.0	34.0	39.0	29.0	41.0
76-77	34.23165719346917	35.5	34.0	38.5	29.0	40.0
78-79	33.86964349545738	35.0	34.0	37.0	29.0	39.0
80-81	33.54848334291023	35.0	34.0	37.0	29.0	39.0
82-83	33.23384173233246	35.0	34.0	36.5	29.0	39.0
84-85	33.069909818575766	35.0	34.0	36.0	29.0	37.0
86-87	32.841894438202175	35.0	34.0	36.0	29.0	37.0
88-89	32.612406228764605	35.0	34.0	35.5	28.0	37.0
90-91	32.416946806135556	35.0	34.0	35.0	28.0	36.0
92-93	32.23126405272295	35.0	34.0	35.0	27.0	36.0
94-95	32.05638184244846	35.0	34.0	35.0	27.0	36.0
96-97	31.892617371880036	35.0	34.0	35.0	27.0	36.0
98-99	31.720974074060518	35.0	33.0	35.0	26.5	35.0
100	31.591978159796312	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.27020803774755464
1101	2	0.34798866985872934
1101	3	0.291223580126605
1101	4	0.3157539093621011
1101	5	0.2566522626169814
1101	6	0.27790967221118734
1101	7	0.3125903932327887
1101	8	0.2602084041605295
1101	9	0.3155585191904464
1101	10-11	0.31682336088211827
1101	12-13	0.14521492163354566
1101	14-15	0.1747496878920458
1101	16-17	0.2368813216812029
1101	18-19	0.17600981021317352
1101	20-21	0.15705810601834003
1101	22-23	0.2067046180328731
1101	24-25	0.2154057102048128
1101	26-27	0.2634858880763886
1101	28-29	0.230727093124365
1101	30-31	0.22884896057379223
1101	32-33	0.25525185917341986
1101	34-35	0.2743566767352874
1101	36-37	0.2782698036354674
1101	38-39	0.23561422189695946
1101	40-41	0.2919346542587107
1101	42-43	0.27789836468385687
1101	44-45	0.2417585524133088
1101	46-47	0.21188411958618758
1101	48-49	0.33392442600106875
1101	50-51	0.3036521265969796
1101	52-53	0.2262339049164126
1101	54-55	0.19949872900982513
1101	56-57	0.21837851764161087
1101	58-59	0.2758004630931765
1101	60-61	0.2460829029448064
1101	62-63	0.23090819791181616
1101	64-65	0.21847649728375984
1101	66-67	0.27159864896099606
1101	68-69	0.28056677057013246
1101	70-71	0.28008852898865655
1101	72-73	0.21023907069032077
1101	74-75	0.20273353749556122
1101	76-77	0.2221480922659751
1101	78-79	0.28656247928679335
1101	80-81	0.2955854492040615
1101	82-83	0.21302246796493307
1101	84-85	0.1672105389489147
1101	86-87	0.19228521556774325
1101	88-89	0.16177867134027935
1101	90-91	0.2706340090601671
1101	92-93	0.20861410866327645
1101	94-95	0.2554792888251214
1101	96-97	0.19933439067127878
1101	98-99	0.23186716725578904
1101	100	0.24247435446612542
1102	1	0.2505020741107806
1102	2	0.269757813818849
1102	3	0.3463924919668244
1102	4	0.31641391051835654
1102	5	0.22116792703430121
1102	6	0.23466957114245446
1102	7	0.2739502495898094
1102	8	0.29313210234584375
1102	9	0.3921550786963195
1102	10-11	0.3903318668767639
1102	12-13	0.38220354187690475
1102	14-15	0.44630591046649215
1102	16-17	0.46108246129433894
1102	18-19	0.4089146941883115
1102	20-21	0.39213806975336496
1102	22-23	0.3679347966943496
1102	24-25	0.4255910986814939
1102	26-27	0.47797774232540746
1102	28-29	0.49632225209106906
1102	30-31	0.5412967661785544
1102	32-33	0.4791397906429111
1102	34-35	0.4756729863830955
1102	36-37	0.3917326910185537
1102	38-39	0.4046382623137603
1102	40-41	0.43789616566469647
1102	42-43	0.43510287046997576
1102	44-45	0.4480775872810838
1102	46-47	0.4043167301263608
1102	48-49	0.46874018876690826
1102	50-51	0.4891462870069958
1102	52-53	0.5026855816368112
1102	54-55	0.4817120487354387
1102	56-57	0.4673154145133438
1102	58-59	0.41842571042651855
1102	60-61	0.44564035128342994
1102	62-63	0.3716844394742438
1102	64-65	0.4423785477653297
1102	66-67	0.5123247972711127
1102	68-69	0.5599119257773424
1102	70-71	0.470912156963216
1102	72-73	0.4418410789652256
1102	74-75	0.47322991517528834
1102	76-77	0.4191508348631814
1102	78-79	0.3697317313200159
1102	80-81	0.40449140455322663
1102	82-83	0.4030436514957145
1102	84-85	0.40446917485517986
1102	86-87	0.4548151286581259
1102	88-89	0.44362556510749585
1102	90-91	0.48548335001141396
1102	92-93	0.49736352844706744
1102	94-95	0.4976212649320715
1102	96-97	0.5068601749949373
1102	98-99	0.4768271812110534
1102	100	0.47198265527560324
1103	1	0.05729843900104825
1103	2	0.13370879481858466
1103	3	0.1861006153469873
1103	4	0.16916256896328719
1103	5	0.10886582329160888
1103	6	0.11143862027109463
1103	7	0.14207566373584513
1103	8	0.1298005328575016
1103	9	0.1797113906394543
1103	10-11	0.2166214203061756
1103	12-13	0.2348269037654518
1103	14-15	0.22564747189834833
1103	16-17	0.29181889041398534
1103	18-19	0.26469335229953117
1103	20-21	0.2713803155888641
1103	22-23	0.22554737688176374
1103	24-25	0.25737820735818673
1103	26-27	0.26438707830926944
1103	28-29	0.28301364436860155
1103	30-31	0.2372852106102883
1103	32-33	0.27364207649067396
1103	34-35	0.25099385521182427
1103	36-37	0.2494395018437956
1103	38-39	0.26249762189501524
1103	40-41	0.2888578584474004
1103	42-43	0.22048933526378534
1103	44-45	0.2059240755610503
1103	46-47	0.251542329078859
1103	48-49	0.1925961922755306
1103	50-51	0.25435770210328457
1103	52-53	0.24176094548943183
1103	54-55	0.2711749817713027
1103	56-57	0.2713171179744478
1103	58-59	0.27652112331720247
1103	60-61	0.24779915453843415
1103	62-63	0.28352953256871416
1103	64-65	0.2587906486953955
1103	66-67	0.20798931395373899
1103	68-69	0.2052998683872289
1103	70-71	0.2622681247182257
1103	72-73	0.16098698072700302
1103	74-75	0.23604059422383017
1103	76-77	0.19972295626021008
1103	78-79	0.19440847891696933
1103	80-81	0.16907673413004431
1103	82-83	0.20443391093983365
1103	84-85	0.19133051598122108
1103	86-87	0.1895728860007253
1103	88-89	0.12983897128286515
1103	90-91	0.05452596355297601
1103	92-93	0.11360520381378336
1103	94-95	0.14873447358858272
1103	96-97	0.22874472418952152
1103	98-99	0.21710068841818853
1103	100	0.17321798238939579
1104	1	0.05115900819201258
1104	2	0.08824494114201897
1104	3	0.19415949234859653
1104	4	0.1445189376487903
1104	5	0.23324773464646142
1104	6	0.16559167650093087
1104	7	0.20284613932695805
1104	8	0.1908616974114281
1104	9	0.27293564069189813
1104	10-11	0.2777497629795107
1104	12-13	0.23092358032153726
1104	14-15	0.25341351223519126
1104	16-17	0.20844584956769552
1104	18-19	0.20989448708530745
1104	20-21	0.21501687447086226
1104	22-23	0.25728017279013926
1104	24-25	0.2621019030038383
1104	26-27	0.2642110166951994
1104	28-29	0.21515150685574014
1104	30-31	0.1785180914278115
1104	32-33	0.2650412793436203
1104	34-35	0.274874303132556
1104	36-37	0.24937132479324475
1104	38-39	0.18329461910798983
1104	40-41	0.1757413930653513
1104	42-43	0.24392550855836959
1104	44-45	0.19009800945178768
1104	46-47	0.10459181612772284
1104	48-49	0.1904782085181509
1104	50-51	0.23409613198331414
1104	52-53	0.14358898948236742
1104	54-55	0.1317806337237144
1104	56-57	0.1592180639622427
1104	58-59	0.15699501625176282
1104	60-61	0.18902604176909676
1104	62-63	0.1679909088960443
1104	64-65	0.1690778913457649
1104	66-67	0.21605743106842823
1104	68-69	0.1956581849884671
1104	70-71	0.2868051201331312
1104	72-73	0.2858021784654312
1104	74-75	0.26892490800808133
1104	76-77	0.2629820190630099
1104	78-79	0.20462404825777725
1104	80-81	0.18239448413753934
1104	82-83	0.13677130992292064
1104	84-85	0.1730023770898299
1104	86-87	0.1583964947393781
1104	88-89	0.1738753523551111
1104	90-91	0.12404033317748286
1104	92-93	0.09945958962097023
1104	94-95	0.1369947850553146
1104	96-97	0.20291985679763158
1104	98-99	0.2443390433783783
1104	100	0.2256878396421378
1105	1	-0.07744993063104388
1105	2	0.024033813715032437
1105	3	-0.009106718232438027
1105	4	-0.01210094419391794
1105	5	-0.03229503381609078
1105	6	0.007463335066574928
1105	7	-0.02781746147289965
1105	8	0.00447577763241469
1105	9	0.0500879133415566
1105	10-11	0.05478697076408423
1105	12-13	0.07309177043563864
1105	14-15	0.131333105324849
1105	16-17	0.10044841457507658
1105	18-19	0.07922472040232975
1105	20-21	0.0716167409083468
1105	22-23	0.14497468268780977
1105	24-25	0.1368360111515301
1105	26-27	0.1652831214810675
1105	28-29	0.13749913365476374
1105	30-31	0.13843833335809563
1105	32-33	0.2383307685049445
1105	34-35	0.22996694315501287
1105	36-37	0.2005253883947944
1105	38-39	0.21563135600881367
1105	40-41	0.21614301023420524
1105	42-43	0.21771595200868177
1105	44-45	0.15182618317412278
1105	46-47	0.19179220163769628
1105	48-49	0.19255136814432916
1105	50-51	0.20597752315833162
1105	52-53	0.2534618015424499
1105	54-55	0.2231279076444892
1105	56-57	0.23503945445426666
1105	58-59	0.252238748868713
1105	60-61	0.20753346169799158
1105	62-63	0.19974795792334987
1105	64-65	0.20747009463397603
1105	66-67	0.17731159066804736
1105	68-69	0.16548681138147714
1105	70-71	0.19898567995736727
1105	72-73	0.26733706598074036
1105	74-75	0.2535536459561527
1105	76-77	0.1870513501500639
1105	78-79	0.24197127664588436
1105	80-81	0.22653423909585513
1105	82-83	0.2182283175913824
1105	84-85	0.1213310433239343
1105	86-87	0.17706783229965595
1105	88-89	0.12767818448752166
1105	90-91	0.16113049166913385
1105	92-93	0.20398032026862722
1105	94-95	0.20901941443830907
1105	96-97	0.1277454097350592
1105	98-99	-0.019335177918012647
1105	100	-0.0501705461486317
1106	1	-0.06633599784093747
1106	2	0.022209991136492135
1106	3	0.012575607859716342
1106	4	-0.009383024407107143
1106	5	-0.043395661853971035
1106	6	0.05585410296151139
1106	7	0.04153954217546385
1106	8	0.0430860792966925
1106	9	-0.009997642465805257
1106	10-11	0.018744843453859517
1106	12-13	0.009219941096496598
1106	14-15	0.06510325421128016
1106	16-17	0.028672557860652148
1106	18-19	0.08938917160450899
1106	20-21	0.14988542832578133
1106	22-23	0.10801300948216408
1106	24-25	0.14226955141394626
1106	26-27	0.1896400822684825
1106	28-29	0.18049627554395897
1106	30-31	0.1662752681717734
1106	32-33	0.1794441360663157
1106	34-35	0.20173600015882442
1106	36-37	0.20601563011803847
1106	38-39	0.20650994380491028
1106	40-41	0.21089627226479024
1106	42-43	0.1965205218071091
1106	44-45	0.1378238020699456
1106	46-47	0.15576942872374389
1106	48-49	0.13805742466399096
1106	50-51	0.14277003849405645
1106	52-53	0.20116479411161237
1106	54-55	0.2101138955080586
1106	56-57	0.13497209133292642
1106	58-59	0.09862140997275048
1106	60-61	0.15552453787029208
1106	62-63	0.16022815589806783
1106	64-65	0.16090995795756413
1106	66-67	0.13845691463698273
1106	68-69	0.22252207415390757
1106	70-71	0.17530239503012268
1106	72-73	0.11317918804050464
1106	74-75	0.14989846441593357
1106	76-77	0.08799311676757071
1106	78-79	0.083315633457552
1106	80-81	0.02874123853848687
1106	82-83	0.0457810932573679
1106	84-85	0.11343672663190318
1106	86-87	0.1185560322345438
1106	88-89	0.1372874078418107
1106	90-91	0.1901953256374327
1106	92-93	0.21546040162585456
1106	94-95	0.22745877414576654
1106	96-97	0.18856522713671708
1106	98-99	0.22698355786979008
1106	100	0.3311711104233872
1107	1	0.11024860616163323
1107	2	0.09811914981131409
1107	3	0.10840746362180909
1107	4	0.0682046075639775
1107	5	-0.018611764135009423
1107	6	-0.009250771777367106
1107	7	0.07395302445105756
1107	8	0.034562575521718486
1107	9	0.06154413995649577
1107	10-11	0.024471003818206327
1107	12-13	0.07604128532184973
1107	14-15	0.08583824595782374
1107	16-17	0.09442517517398841
1107	18-19	0.12121610870934774
1107	20-21	0.0941375987845916
1107	22-23	0.10871175689824497
1107	24-25	0.10959333220803558
1107	26-27	0.11844133735324647
1107	28-29	0.06800527599659034
1107	30-31	0.08855246391339477
1107	32-33	0.04708091888349486
1107	34-35	0.04989406128385099
1107	36-37	0.07628011848777305
1107	38-39	0.015171616690579981
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2216	54-55	-0.3904842302245797
2216	56-57	-0.38551722089333396
2216	58-59	-0.38938662758101117
2216	60-61	-0.34486507533262056
2216	62-63	-0.38684772543058443
2216	64-65	-0.3959178175662075
2216	66-67	-0.3746680223507681
2216	68-69	-0.354164980657913
2216	70-71	-0.33404221402842893
2216	72-73	-0.3603952773346393
2216	74-75	-0.36656221699754354
2216	76-77	-0.42772768883155265
2216	78-79	-0.35635854169069603
2216	80-81	-0.3139864675842574
2216	82-83	-0.28402212609660893
2216	84-85	-0.351711435022807
2216	86-87	-0.37292611202739323
2216	88-89	-0.35372655233428674
2216	90-91	-0.42881298082288666
2216	92-93	-0.3924552565054569
2216	94-95	-0.307202989115801
2216	96-97	-0.32943858055421416
2216	98-99	-0.3967739056718713
2216	100	-0.43647237712702136
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36897.0
3	5768.0
4	3893.0
5	3341.0
6	2644.0
7	2985.0
8	3395.0
9	3873.0
10	4042.0
11	4360.0
12	4811.0
13	5460.0
14	5657.0
15	5855.0
16	5786.0
17	5493.0
18	5582.0
19	5609.0
20	5648.0
21	5984.0
22	7190.0
23	9982.0
24	15683.0
25	20848.0
26	22541.0
27	19258.0
28	20789.0
29	26023.0
30	32103.0
31	38617.0
32	50087.0
33	68727.0
34	100600.0
35	159383.0
36	311947.0
37	667912.0
38	1088817.0
39	490193.0
40	941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80694168987828	19.408078066694383	13.094337710728126	23.69064253269922
2	15.452236037565442	19.871294191115673	40.419020306082245	24.257449465236643
3	18.469760318021354	25.428766598967478	29.570839659147723	26.530633423863442
4	12.37236628601917	16.373835073372216	38.61119013287458	32.642608507734025
5	13.045605563686477	38.35567852831519	35.29809278610116	13.300623121897173
6	31.170934468130824	37.81694126006719	17.756707540783058	13.255416731018933
7	28.158550007844298	32.4821610943678	22.01600846817688	17.343280429611028
8	25.68683037662814	36.64898198628811	19.800035528077252	17.86415210900649
9	25.423751058748124	17.215648869524077	20.554288025272605	36.8063120464552
10-11	24.293916280699516	27.00573071368606	28.42929059927018	20.271062406344242
12-13	24.763340311671996	24.297193596857948	28.58641485638347	22.353051235086586
14-15	22.01435702348525	26.257048375560128	25.47537667767324	26.25321792328138
16-17	21.977157995617166	29.68805891796534	26.085330940477718	22.249452145939777
18-19	21.995076661817393	28.785123345171908	27.75171371449247	21.468086278518225
20-21	22.88305591676114	28.017011091728687	27.408761402011113	21.691171589499064
22-23	22.72794419718752	27.664865702964907	27.419061932111948	22.188128167735627
24-25	22.404922138927393	27.7448391601647	27.433916787137512	22.416321913770393
26-27	22.24214923631143	27.807966729347232	27.547116485436305	22.402767548905032
28-29	22.24766760674027	27.923161687114963	27.522278615108455	22.306892091036318
30-31	22.387220810424257	28.1013094417643	27.280596320670696	22.230873427140747
32-33	22.28399367662425	27.95766530760989	27.55300039112878	22.205340624637085
34-35	22.440169497629487	27.707396232450055	27.560624030598447	22.29181023932201
36-37	22.531389244949033	27.917353354086554	27.26310809985961	22.288149301104802
38-39	22.382204645417932	28.168673636111013	27.317394672826417	22.131727045644638
40-41	22.27667812163365	27.79929579345472	27.58623005026624	22.33779603464539
42-43	22.191861182918657	27.859957760426624	27.537064164991637	22.41111689166308
44-45	22.234836495515786	27.96742088795463	27.54920151599181	22.248541100537775
46-47	22.28871936813908	27.95143954942427	27.462788798118776	22.29705228431788
48-49	22.238518903353082	27.85441287840054	27.5148738163751	22.392194401871272
50-51	22.134816386950867	27.92030362326881	27.525069569648785	22.41981042013154
52-53	22.275074213414122	27.861402156576947	27.49702764783541	22.366495982173518
54-55	22.227197768025096	28.08530771690365	27.39334055245347	22.294153962617777
56-57	22.148492979917286	27.91293675011851	27.471497368365625	22.467072901598577
58-59	22.2062040197379	27.492846751689644	27.718692214665214	22.582257013907245
60-61	22.317155620128716	27.925227739937704	27.43157881885041	22.32603782108317
62-63	22.07377209677904	27.923061109653624	27.469194729417712	22.533972064149623
64-65	22.10954136244742	27.789541863025146	27.54927981669088	22.55163695783655
66-67	22.008964251806685	27.75078287259875	27.517451050145812	22.72280182544875
68-69	22.235767506355582	27.82691080328656	27.461004995645936	22.47631669471192
70-71	22.227490683116066	27.620921097209973	27.516291193446285	22.635297026227676
72-73	22.01802422143058	27.741087658430736	27.603982341738664	22.63690577840002
74-75	22.095920537834065	27.827217464437688	27.473803358731193	22.60305863899706
76-77	22.1420782873507	27.70315238632279	27.407775932045865	22.74699339428064
78-79	22.06777052675852	27.74566350488856	27.55386300670783	22.63270296164509
80-81	22.041256534466978	27.685995187836454	27.602012769966922	22.67073550772965
82-83	22.01411163741571	27.63564603151784	27.706839080512395	22.643403250554055
84-85	22.00691812343231	27.652761236532918	27.554861823715736	22.785458816319032
86-87	22.18792373414848	27.639099258996996	27.52775422904326	22.645222777811266
88-89	21.89011191592693	27.65641615095648	27.537016372908212	22.916455560208377
90-91	21.970958953946113	27.56073159312503	27.598531466525213	22.869777986403644
92-93	22.13419234567871	27.595730901126696	27.521993141411688	22.748083611782903
94-95	21.908136914715655	27.40775647397819	27.62456038722244	23.059546224083714
96-97	22.007778747480348	27.36340075962352	27.683422954095864	22.945397538800268
98-99	21.933229991384103	27.52639172092424	27.582289765820306	22.95808852187135
100	21.986152406542782	27.137706216889473	27.621022998138884	23.25511837842886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2701.0
1	1641.5
2	562.5
3	488.5
4	399.0
5	340.0
6	277.5
7	261.5
8	291.0
9	272.5
10	270.0
11	293.5
12	289.5
13	302.0
14	346.5
15	399.5
16	476.0
17	619.5
18	835.0
19	1165.5
20	1701.0
21	2559.0
22	3823.5
23	5951.5
24	8792.5
25	12298.0
26	17210.5
27	23202.5
28	30645.0
29	39657.0
30	48330.0
31	56402.0
32	64488.5
33	72535.0
34	80487.5
35	87216.0
36	93478.0
37	100152.0
38	105973.0
39	109406.5
40	113341.0
41	117784.5
42	119353.5
43	120330.5
44	119278.5
45	117522.5
46	117677.0
47	115427.5
48	113126.5
49	110757.5
50	107001.5
51	103946.5
52	97643.0
53	92220.0
54	89208.0
55	83933.0
56	76652.5
57	68978.5
58	62445.0
59	58274.5
60	53342.0
61	46236.5
62	40473.5
63	34570.5
64	28265.5
65	22953.5
66	18151.0
67	13809.0
68	10463.5
69	7954.5
70	5839.5
71	4379.5
72	3438.5
73	2561.5
74	1888.0
75	1366.5
76	911.5
77	618.5
78	418.0
79	267.0
80	179.5
81	117.0
82	86.5
83	68.0
84	50.5
85	43.0
86	39.0
87	27.5
88	23.5
89	22.0
90	13.5
91	9.5
92	11.0
93	9.0
94	6.0
95	6.0
96	4.0
97	1.0
98	1.0
99	0.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07155222580491678
2	0.07365670303447316
3	0.07521218620414527
4	0.07783515782359235
5	0.07560868191406168
6	0.07582217960401669
7	0.07496818884419672
8	0.07448019412429958
9	0.07469369181425457
10-11	0.07412944791937351
12-13	0.07304670963460176
14-15	0.07162847497990071
16-17	0.07027123966518682
18-19	0.06979849478028648
20-21	0.06934099973038291
22-23	0.07106423108501966
24-25	0.07147597662993287
26-27	0.07085073339506467
28-29	0.06732802151080726
30-31	0.0721622192047882
32-33	0.07043898785015146
34-35	0.06764826804573974
36-37	0.0647812990663441
38-39	0.06659602943096156
40-41	0.06924950072040222
42-43	0.07358045385948923
44-45	0.07481569049422886
46-47	0.07807915518354092
48-49	0.08128162053286583
50-51	0.08205936211770189
52-53	0.08231860931264724
54-55	0.08239485848763117
56-57	0.08000063439313587
58-59	0.07899414528334803
60-61	0.07652367201386881
62-63	0.07745391194867271
64-65	0.0765389218488656
66-67	0.0758984287790006
68-69	0.07536468455411312
70-71	0.0736262033644796
72-73	0.07351945451950209
74-75	0.07312295880958568
76-77	0.07309245913959211
78-79	0.07329070699455031
80-81	0.07451069379429315
82-83	0.07383970105443459
84-85	0.07326020732455675
86-87	0.07385495088943138
88-89	0.07124722910498107
90-91	0.06886825484548258
92-93	0.06856325814554687
94-95	0.06895975385546328
96-97	0.06809051326064652
98-99	0.06780076639570759
100	0.06703827464586834
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3278724.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.41304360185777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63731250095312	42.85155276942705
2	14.523329139905014	16.67657058306996
3	4.643438721984899	7.9978184942362125
4	2.0624865521312055	4.736545213830167
5	1.0995351270424678	3.156382909533171
6	0.6905469845359855	2.3787842479377566
7	0.45953534223613524	1.8468325848278504
8	0.3066287958704354	1.4083593941515562
9	0.23604815764636908	1.2197018850380266
>10	1.2502418442018535	12.79810120923948
>50	0.0669927874942258	2.599430284643582
>100	0.02363822584242237	2.146110385836636
>500	1.0632806228940077E-4	0.0501189111222858
>1k	1.594920934279289E-4	0.1336911271062871
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.049966999357067E-5	0.0
12-13	0.0	0.0	0.0	3.049966999357067E-5	0.0
14-15	0.0	0.0	0.0	4.574950499035601E-5	0.0
16-17	0.0	0.0	0.0	6.099933998714134E-5	0.0
18-19	0.0	0.0	0.0	6.099933998714134E-5	0.0
20-21	0.0	0.0	0.0	6.099933998714134E-5	0.0
22-23	0.0	0.0	0.0	9.149900998071202E-5	0.0
24-25	0.0	0.0	0.0	2.4399735994856534E-4	0.0
26-27	0.0	0.0	0.0	4.7274488490034537E-4	0.0
28-29	0.0	0.0	0.0	0.0015402333346753187	0.0
30-31	0.0	0.0	0.0	0.00588643630875914	0.0
32-33	0.0	0.0	0.0	0.01477709011188499	0.0
34-35	0.0	0.0	0.0	0.02827319408404001	0.0
36-37	0.0	0.0	0.0	0.05187993865906371	0.0
38-39	0.0	0.0	0.0	0.09163625849568308	0.0
40-41	0.0	0.0	0.0	0.13884974764573046	0.0
42-43	0.0	0.0	0.0	0.18729847343051748	0.0
44-45	0.0	0.0	0.0	0.24231987809891897	0.0
46-47	0.0	3.049966999357067E-5	0.0	0.3107001382245044	0.0
48-49	0.0	3.049966999357067E-5	0.0	0.38679681485846323	0.0
50-51	0.0	6.099933998714134E-5	0.0	0.47411737005005605	0.0
52-53	0.0	7.624917498392668E-5	0.0	0.5628256602263564	0.0
54-55	0.0	9.1499009980712E-5	0.0	0.6550871619569076	0.0
56-57	0.0	9.1499009980712E-5	0.0	0.7677224432431641	0.0
58-59	0.0	9.1499009980712E-5	0.0	0.8987337757005469	0.0
60-61	0.0	9.1499009980712E-5	0.0	1.0397794995858145	0.0
62-63	0.0	1.0674884497749734E-4	0.0	1.1819994607658346	0.0
64-65	0.0	1.2199867997428268E-4	0.0	1.3315088430743178	0.0
66-67	0.0	1.2199867997428268E-4	0.0	1.5051739640177093	0.0
68-69	0.0	1.2199867997428268E-4	0.0	1.7049315526406006	0.0
70-71	0.0	1.2199867997428268E-4	0.0	1.9194204818703862	0.0
72-73	0.0	1.2199867997428268E-4	0.0	2.1468107715074525	0.0
74-75	0.0	1.2199867997428268E-4	0.0	2.370922346620209	0.0
76-77	0.0	1.2199867997428268E-4	0.0	2.6059833032606585	0.0
78-79	0.0	1.2199867997428268E-4	0.0	2.878330106468248	0.0
80-81	0.0	1.2199867997428268E-4	0.0	3.1760678849454846	0.0
82-83	0.0	1.2199867997428268E-4	0.0	3.490549982249192	0.0
84-85	0.0	1.2199867997428268E-4	0.0	3.796293924099741	0.0
86-87	0.0	1.2199867997428268E-4	0.0	4.118492437911822	0.0
88	0.0	1.2199867997428268E-4	0.0	4.385364550355565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11350	0.0	28.739132	1
TACATGG	11805	0.0	26.317554	2
GTATCAA	6475	0.0	26.204597	1
ACATGGG	11595	0.0	25.334906	3
TCAACGC	6545	0.0	25.20621	4
ATCAACG	6735	0.0	24.564907	3
CAACGCA	6730	0.0	24.51257	5
AACGCAG	6920	0.0	23.771618	6
GGTATCA	4540	0.0	23.190077	1
TATCAAC	7630	0.0	21.991442	2
CATGGGG	7795	0.0	21.767128	4
ACGCAGA	8170	0.0	19.84695	7
ACCGCGT	215	1.1954035E-6	19.674372	8
GAGTACA	8150	0.0	19.319561	1
CGCAGAG	8530	0.0	19.064434	8
TACCTGG	2145	0.0	18.844353	2
AGTACAT	8480	0.0	17.902624	2
GTATAGA	1355	0.0	17.690533	1
GCGTTAG	160	0.001279038	17.625496	1
CATGGGA	3735	0.0	17.11427	4
>>END_MODULE