FastQCFastQC Report
Wed 25 May 2016
SRR1032966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278724
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66580.2030668028171935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53420.16292923710565452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61500.037.7346421
GTATCAA100150.035.790021
TCAACGC114800.030.4842974
ATCAACG115750.030.27523
CAACGCA118500.029.490525
AACGCAG121200.028.9111656
TATCAAC123600.028.4661182
GTACATG111200.025.9389231
ACGCAGA140150.024.9345237
TACATGG112350.024.7015422
CGCAGAG145500.023.920718
ACATGGG113600.023.8090083
GCAGAGT159350.021.5778249
CCGGTCG3550.021.1709469
CATGGGG75400.020.77394
AGAGTAC131050.020.3230110-11
GAGTACA79650.019.8761621
GAGTACT87450.019.7933712-13
TACGTCG2702.5784175E-819.1620035
GTATAGA13550.018.7215961
Produced by FastQC (version 0.11.5)