##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1032966_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3278724 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84396521329639 34.0 31.0 34.0 31.0 34.0 2 32.88219319466963 34.0 31.0 34.0 31.0 34.0 3 32.9701026984888 34.0 31.0 34.0 31.0 34.0 4 36.37737821176775 37.0 37.0 37.0 35.0 37.0 5 36.36095810443331 37.0 37.0 37.0 35.0 37.0 6 36.41593314960332 37.0 37.0 37.0 35.0 37.0 7 36.381806458854115 37.0 37.0 37.0 35.0 37.0 8 36.31954839748634 37.0 37.0 37.0 35.0 37.0 9 38.163914071449746 39.0 39.0 39.0 37.0 39.0 10-11 38.17495906944286 39.0 39.0 39.0 37.0 39.0 12-13 38.168545598836616 39.0 39.0 39.0 37.0 39.0 14-15 39.71674819228456 41.0 40.0 41.0 37.0 41.0 16-17 39.72697457913505 41.0 40.0 41.0 37.0 41.0 18-19 39.75135891279656 41.0 40.0 41.0 37.0 41.0 20-21 39.76548879991118 41.0 40.0 41.0 37.0 41.0 22-23 39.732522621605234 41.0 40.0 41.0 37.0 41.0 24-25 39.7132520151132 41.0 40.0 41.0 37.0 41.0 26-27 39.642356599701586 41.0 40.0 41.0 37.0 41.0 28-29 39.583875007472415 41.0 40.0 41.0 37.0 41.0 30-31 39.48089668419787 41.0 40.0 41.0 36.5 41.0 32-33 39.36660633831942 41.0 39.0 41.0 35.5 41.0 34-35 39.281625260314684 41.0 39.0 41.0 35.0 41.0 36-37 39.19762322171674 41.0 39.0 41.0 35.0 41.0 38-39 39.112319914698524 41.0 39.0 41.0 35.0 41.0 40-41 39.01787585658323 41.0 39.0 41.0 35.0 41.0 42-43 38.887064906957704 41.0 39.0 41.0 35.0 41.0 44-45 38.72419666919204 41.0 39.0 41.0 35.0 41.0 46-47 38.51034701304532 41.0 38.5 41.0 35.0 41.0 48-49 38.31194299977675 41.0 38.0 41.0 34.5 41.0 50-51 38.15924213200013 40.0 38.0 41.0 34.0 41.0 52-53 38.01742888391948 40.0 38.0 41.0 34.0 41.0 54-55 37.864822107624796 40.0 37.0 41.0 34.0 41.0 56-57 37.650269281586375 40.0 37.0 41.0 33.5 41.0 58-59 37.47453918048607 40.0 36.5 41.0 33.0 41.0 60-61 37.2774872175883 40.0 36.0 41.0 33.0 41.0 62-63 37.037440174897306 39.0 35.5 41.0 33.0 41.0 64-65 36.743621756512596 39.0 35.0 41.0 32.5 41.0 66-67 36.45959068222882 38.5 35.0 41.0 32.0 41.0 68-69 36.16014964968079 37.5 35.0 40.0 32.0 41.0 70-71 35.83438999440026 37.0 35.0 40.0 31.5 41.0 72-73 35.49143111771531 36.5 35.0 39.0 31.0 41.0 74-75 35.143582991432034 36.0 35.0 39.0 31.0 41.0 76-77 34.79949364447877 35.5 35.0 38.5 31.0 40.0 78-79 34.45551836629127 35.0 35.0 37.0 31.0 39.0 80-81 34.12984853253887 35.0 34.5 37.0 30.5 39.0 82-83 33.82949266238939 35.0 34.0 36.5 30.0 39.0 84-85 33.559147552523484 35.0 34.0 36.0 30.0 37.0 86-87 33.41905570581726 35.0 34.0 36.0 30.0 37.0 88-89 33.235175330402924 35.0 34.0 35.5 30.0 37.0 90-91 33.069706995770304 35.0 34.0 35.0 30.0 36.0 92-93 32.913189704287404 35.0 34.0 35.0 30.0 36.0 94-95 32.81896615878617 35.0 34.0 35.0 30.0 36.0 96-97 32.76409862495288 35.0 34.0 35.0 30.0 36.0 98-99 32.65983092812936 35.0 34.0 35.0 30.0 36.0 100 32.58193217849383 35.0 34.0 35.0 30.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19026945780348115 1101 2 0.24663586526490633 1101 3 0.1990453998220474 1101 4 0.14463686279798083 1101 5 0.13821203172794583 1101 6 0.10498639203814975 1101 7 0.09593162808514677 1101 8 0.08291366815630852 1101 9 0.10673065660044756 1101 10-11 0.12997214384076727 1101 12-13 0.1880480007894363 1101 14-15 0.24654660461643374 1101 16-17 0.2625412300232952 1101 18-19 0.2626160833069804 1101 20-21 0.27245648015789925 1101 22-23 0.2376845052909502 1101 24-25 0.24842977471915617 1101 26-27 0.2793975513656406 1101 28-29 0.28558012091846763 1101 30-31 0.23412255074778443 1101 32-33 0.25186538924761237 1101 34-35 0.24938574540309588 1101 36-37 0.29750544521730404 1101 38-39 0.23384368176893133 1101 40-41 0.2445703990200414 1101 42-43 0.23362238725168538 1101 44-45 0.19785521976662324 1101 46-47 0.1566313413502911 1101 48-49 0.06871471165322873 1101 50-51 0.07979597835937113 1101 52-53 0.14617640521897357 1101 54-55 0.09477622280125075 1101 56-57 0.16550233949751458 1101 58-59 0.14730620155995666 1101 60-61 0.06807128665569451 1101 62-63 0.09495491335234618 1101 64-65 0.1465810254950739 1101 66-67 0.21933820470070486 1101 68-69 0.10318012571602253 1101 70-71 0.09629375072040602 1101 72-73 0.15881137797046563 1101 74-75 0.1669170487287417 1101 76-77 0.19853345938391698 1101 78-79 0.14138625609407285 1101 80-81 0.10719537467414852 1101 82-83 0.1794651687191262 1101 84-85 0.15085208724936194 1101 86-87 0.13442438474067586 1101 88-89 0.1354396988037152 1101 90-91 0.16170367365793226 1101 92-93 0.184042523476883 1101 94-95 0.2075529594742278 1101 96-97 0.2205065486520965 1101 98-99 0.18976867070644943 1101 100 0.1989489120817396 1102 1 0.1913832724129989 1102 2 0.19541409451689162 1102 3 0.17740053658598498 1102 4 0.1193389349342695 1102 5 0.15000449795677184 1102 6 0.12179770756582542 1102 7 0.16317505221793027 1102 8 0.051030265464369506 1102 9 0.12100472717678912 1102 10-11 0.1588510374716421 1102 12-13 0.18423118903947966 1102 14-15 0.23962511400966235 1102 16-17 0.23400252939347155 1102 18-19 0.2521559839692884 1102 20-21 0.26742426374703143 1102 22-23 0.279060479043288 1102 24-25 0.25293674717187287 1102 26-27 0.2632185837812244 1102 28-29 0.2745870079887567 1102 30-31 0.23701210264543704 1102 32-33 0.28236095153351215 1102 34-35 0.3034150273986995 1102 36-37 0.31374581797258116 1102 38-39 0.31466600310829307 1102 40-41 0.2780342120756387 1102 42-43 0.29879056724251996 1102 44-45 0.3682315106536933 1102 46-47 0.4341099464520468 1102 48-49 0.4170459446790886 1102 50-51 0.39655576367018597 1102 52-53 0.31667066086966855 1102 54-55 0.3091426768305823 1102 56-57 0.3616851768608669 1102 58-59 0.3505625804293544 1102 60-61 0.36310650732498573 1102 62-63 0.3275448347503911 1102 64-65 0.39290699121044526 1102 66-67 0.3540970736959892 1102 68-69 0.32926335470233425 1102 70-71 0.3063392022430733 1102 72-73 0.3125878878776689 1102 74-75 0.3167971290249838 1102 76-77 0.3117351032713529 1102 78-79 0.293575003726005 1102 80-81 0.3242693372442318 1102 82-83 0.24173594363400497 1102 84-85 0.2706103831297213 1102 86-87 0.26864982242170043 1102 88-89 0.27672756657891995 1102 90-91 0.2753034068503766 1102 92-93 0.2692053967242458 1102 94-95 0.2869543568052819 1102 96-97 0.3207400052636231 1102 98-99 0.3644356374647728 1102 100 0.369529302060549 1103 1 0.10376006148415229 1103 2 0.16058387651519013 1103 3 0.16755240205069555 1103 4 0.046234869668829504 1103 5 0.0950094001746109 1103 6 0.09063644605117105 1103 7 0.11416272596063948 1103 8 0.05975716664297437 1103 9 0.10269379149674762 1103 10-11 0.11688980074616495 1103 12-13 0.1239228657568816 1103 14-15 0.1243015120157267 1103 16-17 0.20309138427670348 1103 18-19 0.19052962354425063 1103 20-21 0.1743206593059483 1103 22-23 0.19784479258184007 1103 24-25 0.15522227712784087 1103 26-27 0.13608786697686526 1103 28-29 0.1665174171531305 1103 30-31 0.15663527543709677 1103 32-33 0.18527463277335698 1103 34-35 0.17831336795426012 1103 36-37 0.08902004819726272 1103 38-39 0.12574708118306432 1103 40-41 0.07961706432421778 1103 42-43 0.12288596106461114 1103 44-45 0.1618236683763925 1103 46-47 0.16858525023859272 1103 48-49 0.1321702798604818 1103 50-51 0.10779662067638895 1103 52-53 0.15155435038845866 1103 54-55 0.14323271555571893 1103 56-57 0.22783190651745855 1103 58-59 0.2002628062332974 1103 60-61 0.20007375860298282 1103 62-63 0.17607663242624483 1103 64-65 0.24083034181673924 1103 66-67 0.29837093367212475 1103 68-69 0.3004167027502689 1103 70-71 0.2677593040375825 1103 72-73 0.2725915109106154 1103 74-75 0.19238297186480224 1103 76-77 0.2525124186357104 1103 78-79 0.263033312627698 1103 80-81 0.2730382211117188 1103 82-83 0.2836003277899053 1103 84-85 0.21338894748701875 1103 86-87 0.21308632161842667 1103 88-89 0.2020980932058265 1103 90-91 0.20714954871754543 1103 92-93 0.25300352704855555 1103 94-95 0.22752755875599462 1103 96-97 0.21228197126749393 1103 98-99 0.19927252947456253 1103 100 0.1770264377987445 1104 1 0.08920563472078413 1104 2 0.1316932894433549 1104 3 0.12255055402808068 1104 4 0.08359339604560745 1104 5 0.052079335343975686 1104 6 0.06422320821565819 1104 7 0.06188865921205888 1104 8 0.027593082213307696 1104 9 0.050123042198499945 1104 10-11 0.10401682483039565 1104 12-13 0.10784631766334485 1104 14-15 0.15016883398122616 1104 16-17 0.13056298965894086 1104 18-19 0.13363924624795231 1104 20-21 0.16781611916390204 1104 22-23 0.15001846675047403 1104 24-25 0.17026593041882165 1104 26-27 0.15645282647020053 1104 28-29 0.16334156531459598 1104 30-31 0.1345531533077775 1104 32-33 0.13412985768270147 1104 34-35 0.12643288007629394 1104 36-37 0.12407429015836868 1104 38-39 0.1797083426333188 1104 40-41 0.16914751674013928 1104 42-43 0.22199091875987875 1104 44-45 0.2598350462021273 1104 46-47 0.22301451092965152 1104 48-49 0.22688418598093563 1104 50-51 0.22642069291908484 1104 52-53 0.275445037353407 1104 54-55 0.28359253055366906 1104 56-57 0.2672799471140195 1104 58-59 0.23944538087842204 1104 60-61 0.29117078478496694 1104 62-63 0.19135153827698304 1104 64-65 0.25899665386285164 1104 66-67 0.29246175649171136 1104 68-69 0.31826660335661927 1104 70-71 0.27078431727342434 1104 72-73 0.2894439787700094 1104 74-75 0.2648606707270176 1104 76-77 0.2852016911141604 1104 78-79 0.28462371090154903 1104 80-81 0.29093656987853933 1104 82-83 0.30069178992542334 1104 84-85 0.3220356954666812 1104 86-87 0.2953520472919635 1104 88-89 0.28658219521667405 1104 90-91 0.2781725541017792 1104 92-93 0.2991406603159632 1104 94-95 0.2910835538412542 1104 96-97 0.24437712944824597 1104 98-99 0.30115976013775025 1104 100 0.2860852448751743 1105 1 0.09317892980666898 1105 2 0.12938364448439188 1105 3 0.11850952908755374 1105 4 0.04332657456784261 1105 5 0.09272161167419313 1105 6 0.0677146158203712 1105 7 0.078291995239411 1105 8 0.04187497500662829 1105 9 0.07792889845943307 1105 10-11 0.09473261745941386 1105 12-13 0.07127912092045108 1105 14-15 0.11661153680083203 1105 16-17 0.13280670709833942 1105 18-19 0.12892149191949898 1105 20-21 0.09200905249268487 1105 22-23 0.13203154221709212 1105 24-25 0.15740503653651672 1105 26-27 0.107798127308385 1105 28-29 0.10843105599367675 1105 30-31 0.14525390118195958 1105 32-33 0.17783382699367678 1105 34-35 0.13521101073230568 1105 36-37 0.12038595373260108 1105 38-39 0.17787271179706465 1105 40-41 0.21188296810174734 1105 42-43 0.1927149262424166 1105 44-45 0.1403441204848832 1105 46-47 0.19214164954249924 1105 48-49 0.19194677443764618 1105 50-51 0.17220142652637804 1105 52-53 0.10072202779534223 1105 54-55 0.17426639517743325 1105 56-57 0.16970731301080377 1105 58-59 0.14425505429531427 1105 60-61 0.19814544205672746 1105 62-63 0.202955730318628 1105 64-65 0.15653988144654107 1105 66-67 0.27569871232880416 1105 68-69 0.26343767341243307 1105 70-71 0.21192821191081634 1105 72-73 0.163185474594286 1105 74-75 0.20202719130968916 1105 76-77 0.2085496138755616 1105 78-79 0.1893978119157893 1105 80-81 0.15050706394601576 1105 82-83 0.1734520565951243 1105 84-85 0.15326001219197138 1105 86-87 0.15414478634799167 1105 88-89 0.18108260690794253 1105 90-91 0.11905738678817102 1105 92-93 0.1526660277077312 1105 94-95 0.17313039937544517 1105 96-97 0.14593434694644714 1105 98-99 0.1446262006587986 1105 100 0.10558556170671096 1106 1 0.08357338981500106 1106 2 0.1430103449254858 1106 3 0.08661910952710627 1106 4 0.046202945917229954 1106 5 0.08848181574944647 1106 6 0.07206143017157984 1106 7 0.07590227422309681 1106 8 0.014987231576540694 1106 9 0.11067604584019364 1106 10-11 0.1310103013895798 1106 12-13 0.05642601699318561 1106 14-15 0.0816903795124162 1106 16-17 0.1010495806176479 1106 18-19 0.09817552660793893 1106 20-21 0.10112229432861142 1106 22-23 0.10429733973519006 1106 24-25 0.14247351047072243 1106 26-27 0.15372244862761875 1106 28-29 0.15739002163758897 1106 30-31 0.13093961739642168 1106 32-33 0.1300768207022287 1106 34-35 0.12210600837762797 1106 36-37 0.1494546719742047 1106 38-39 0.13872885831578685 1106 40-41 0.07856423874309115 1106 42-43 0.06316057115054718 1106 44-45 0.09636615494044065 1106 46-47 0.07687637510653644 1106 48-49 0.011859697959607729 1106 50-51 0.013659565757656367 1106 52-53 0.004012150150842331 1106 54-55 -0.011636643895847953 1106 56-57 -0.05154995750052649 1106 58-59 -0.09746106318868897 1106 60-61 -0.08267836498471581 1106 62-63 -0.0945923511807436 1106 64-65 -0.08453258516343709 1106 66-67 -0.059301956480545925 1106 68-69 0.00927636659645259 1106 70-71 0.06835334910602597 1106 72-73 0.022939545625234814 1106 74-75 0.060713622944781775 1106 76-77 0.014877112941100279 1106 78-79 0.029932989769967833 1106 80-81 0.005070250178789593 1106 82-83 0.11573454577318643 1106 84-85 0.07340477771847986 1106 86-87 0.10010586092907658 1106 88-89 0.03907616455047247 1106 90-91 0.022552594309267704 1106 92-93 0.0245797046894225 1106 94-95 -0.02325194027147859 1106 96-97 -0.030903624209209113 1106 98-99 0.014268135844829999 1106 100 7.276841415020385E-4 1107 1 0.011687329965702986 1107 2 0.040815304176462064 1107 3 0.02409423494710694 1107 4 -0.0054315648622775825 1107 5 0.020203922878614833 1107 6 0.03890147407062727 1107 7 -0.008643788360750193 1107 8 0.013486298000898955 1107 9 0.021702292254985878 1107 10-11 -0.02049009208535324 1107 12-13 -0.013189013929782334 1107 14-15 0.05632350927331231 1107 16-17 0.06809026037285548 1107 18-19 0.04365979919042218 1107 20-21 0.07448593522779845 1107 22-23 0.042590875351727675 1107 24-25 0.05194521253486073 1107 26-27 0.08417994251239236 1107 28-29 0.05897822876339376 1107 30-31 0.06967191654126736 1107 32-33 0.08330800789109105 1107 34-35 0.12133358029624475 1107 36-37 0.04678953344929937 1107 38-39 0.046114931005604376 1107 40-41 0.08124877045622725 1107 42-43 0.07204436623356258 1107 44-45 0.01651604738895429 1107 46-47 -0.008914125753442192 1107 48-49 0.043312809715182254 1107 50-51 0.1165958684652324 1107 52-53 0.1465543113161374 1107 54-55 0.19865873487215424 1107 56-57 0.17618712264945202 1107 58-59 0.1319518843241596 1107 60-61 0.1474471792192844 1107 62-63 0.11480410665804897 1107 64-65 0.08401822038732121 1107 66-67 0.057624362728041945 1107 68-69 0.06450496390782234 1107 70-71 0.14453136024840774 1107 72-73 0.11026531953805829 1107 74-75 0.09177325977265838 1107 76-77 0.0320506343544551 1107 78-79 0.036576753924762784 1107 80-81 0.09946650984055339 1107 82-83 0.10196691626596532 1107 84-85 0.09301362795586954 1107 86-87 0.08061253340053298 1107 88-89 0.08753719485499545 1107 90-91 0.07252784004352719 1107 92-93 0.07660350829384299 1107 94-95 0.1052952236975031 1107 96-97 0.1127301078165246 1107 98-99 0.13372742231756973 1107 100 0.15801228304569293 1108 1 0.05345100882406939 1108 2 -0.005672916854166488 1108 3 0.01686607357848402 1108 4 -0.007544122944189269 1108 5 -0.023117829769475406 1108 6 0.008143878455925346 1108 7 -0.003806860407557622 1108 8 0.008610554554827843 1108 9 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30-31 -0.25109868150777714 2214 32-33 -0.30300031178406783 2214 34-35 -0.29373764497332644 2214 36-37 -0.25887106223100886 2214 38-39 -0.24681362753058522 2214 40-41 -0.23792864679279546 2214 42-43 -0.3072993539117661 2214 44-45 -0.28597775185898655 2214 46-47 -0.32821358095011277 2214 48-49 -0.2548477935957081 2214 50-51 -0.3197114343699141 2214 52-53 -0.34196745127192685 2214 54-55 -0.3586027493582691 2214 56-57 -0.31572215752624544 2214 58-59 -0.2956506507865697 2214 60-61 -0.34051701895882047 2214 62-63 -0.3243541720502847 2214 64-65 -0.34336788041468935 2214 66-67 -0.34608051182458865 2214 68-69 -0.31375523005037564 2214 70-71 -0.33162310724757305 2214 72-73 -0.2550221737105858 2214 74-75 -0.3078191571456017 2214 76-77 -0.3019865534563664 2214 78-79 -0.30356269136347436 2214 80-81 -0.3333826453231623 2214 82-83 -0.28337850908403794 2214 84-85 -0.3025095598886338 2214 86-87 -0.26416524556232446 2214 88-89 -0.28285461228848163 2214 90-91 -0.284581498858536 2214 92-93 -0.2820994229455138 2214 94-95 -0.32526206326774343 2214 96-97 -0.3387561984694685 2214 98-99 -0.33057520051576006 2214 100 -0.37079105608540175 2215 1 -0.163227718956378 2215 2 -0.14837556238462213 2215 3 -0.1596321125975706 2215 4 -0.09893739337856289 2215 5 -0.15031069527194063 2215 6 -0.14003586726806816 2215 7 -0.1422702115693113 2215 8 -0.09133236992418858 2215 9 -0.1407371870881775 2215 10-11 -0.20124835809595254 2215 12-13 -0.18424196479013943 2215 14-15 -0.2589932243185231 2215 16-17 -0.22311925589946213 2215 18-19 -0.24099259716797405 2215 20-21 -0.21714931182363983 2215 22-23 -0.22032934838633622 2215 24-25 -0.2674892946428713 2215 26-27 -0.23811381412869537 2215 28-29 -0.18023723078368903 2215 30-31 -0.25020022363720784 2215 32-33 -0.2513174216218985 2215 34-35 -0.2511469885450026 2215 36-37 -0.1990835317112314 2215 38-39 -0.20423221712496797 2215 40-41 -0.19024895473783943 2215 42-43 -0.19305202151296896 2215 44-45 -0.1974359837276367 2215 46-47 -0.13632642045534027 2215 48-49 -0.06482753285347087 2215 50-51 -0.08696219779876202 2215 52-53 -0.08733682191068226 2215 54-55 -0.1288640480048855 2215 56-57 -0.0944742862752932 2215 58-59 -0.07772611333430746 2215 60-61 -0.0910749882651487 2215 62-63 -0.059966666924786693 2215 64-65 -0.12898584734096374 2215 66-67 -0.1292417019836236 2215 68-69 -0.13116168248829752 2215 70-71 -0.18668275755777586 2215 72-73 -0.2213162032226208 2215 74-75 -0.1892830852786389 2215 76-77 -0.16116426962391728 2215 78-79 -0.14891724638427206 2215 80-81 -0.16542880773327084 2215 82-83 -0.1893499340104441 2215 84-85 -0.14483103478340809 2215 86-87 -0.2355921915279353 2215 88-89 -0.28484232856951763 2215 90-91 -0.3162691436470695 2215 92-93 -0.24424958566314814 2215 94-95 -0.2141251588520845 2215 96-97 -0.21951186477701867 2215 98-99 -0.2213271087890618 2215 100 -0.23423548887652856 2216 1 -0.2146951027234536 2216 2 -0.21360693099398986 2216 3 -0.21475215108765866 2216 4 -0.16634223763389855 2216 5 -0.17425687140341495 2216 6 -0.14334865885750503 2216 7 -0.13082964124023988 2216 8 -0.10242636610503553 2216 9 -0.18661939908453462 2216 10-11 -0.2138542591192092 2216 12-13 -0.23418411099558512 2216 14-15 -0.31312904019991805 2216 16-17 -0.30781594801992185 2216 18-19 -0.304683889983707 2216 20-21 -0.3132252310061858 2216 22-23 -0.3063513765902073 2216 24-25 -0.30486539763832354 2216 26-27 -0.3514421699135184 2216 28-29 -0.38385772111899996 2216 30-31 -0.36003254906374593 2216 32-33 -0.39073682444111313 2216 34-35 -0.30863828623451184 2216 36-37 -0.3740309695389783 2216 38-39 -0.3506877870821228 2216 40-41 -0.2694820516248626 2216 42-43 -0.3174960885868785 2216 44-45 -0.34201090027693226 2216 46-47 -0.34316133370527524 2216 48-49 -0.3497171857830139 2216 50-51 -0.3039936047093974 2216 52-53 -0.26555656834405283 2216 54-55 -0.27067554478953326 2216 56-57 -0.32574054629188964 2216 58-59 -0.34962809016191443 2216 60-61 -0.39084125102485245 2216 62-63 -0.27818286136865567 2216 64-65 -0.28301854835466855 2216 66-67 -0.3015089187663662 2216 68-69 -0.32177755332345725 2216 70-71 -0.3047506845087611 2216 72-73 -0.3127460333765413 2216 74-75 -0.3043868489202808 2216 76-77 -0.3360425686904591 2216 78-79 -0.325002341539701 2216 80-81 -0.3174139220393357 2216 82-83 -0.3727551130509994 2216 84-85 -0.3341445094673787 2216 86-87 -0.3252381676431 2216 88-89 -0.37824749357562126 2216 90-91 -0.31771734488462045 2216 92-93 -0.3590070430867982 2216 94-95 -0.3582674190937496 2216 96-97 -0.3659136955826412 2216 98-99 -0.35034618682578156 2216 100 -0.331683147108798 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 6.0 4 11.0 5 30.0 6 68.0 7 176.0 8 349.0 9 680.0 10 1093.0 11 1813.0 12 2879.0 13 4594.0 14 5265.0 15 6845.0 16 8827.0 17 13416.0 18 16311.0 19 9160.0 20 6672.0 21 6264.0 22 6609.0 23 6870.0 24 7615.0 25 9056.0 26 11170.0 27 14651.0 28 18671.0 29 23829.0 30 30866.0 31 38950.0 32 50148.0 33 65165.0 34 92116.0 35 144285.0 36 283269.0 37 655472.0 38 1160276.0 39 573365.0 40 1872.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67699316531436 19.44129365236463 13.321979298609794 23.55973388371122 2 15.688517740208802 20.01320641349799 40.017598384730576 24.28067746156263 3 18.565335597632977 25.405387105569922 29.94508829365624 26.08418900314086 4 12.485499622830053 16.50976417193581 38.27102123834365 32.73371496689049 5 12.79355721237501 38.02659891634044 35.39708512964722 13.782758741637323 6 31.14366288599484 37.754063769785084 17.39287977878403 13.709393565436043 7 27.816004240697396 32.617044651152014 22.007310201036628 17.559640907113966 8 25.88909538200293 35.98782013007539 19.707597606173007 18.41548688174867 9 25.68496211133427 16.315047038675566 20.758901815819453 37.24108903417071 10-11 24.466469272802467 26.538540602990672 28.31348414810152 20.68150597610534 12-13 25.251656934971106 23.829836617618554 28.806749098237894 22.111757349172446 14-15 22.210305305212188 25.646982861294305 26.201147460334358 25.941564373159153 16-17 21.60322125314604 29.227742255828794 26.761843936848607 22.407192554176564 18-19 21.544731812861002 27.829734060690793 29.268680624561185 21.35685350188702 20-21 22.37670813401799 27.025223837078084 29.190578407941626 21.4074896209623 22-23 22.174525690705046 26.776404780531397 29.17736064281281 21.871708885950746 24-25 21.906525808530024 26.88939637128904 29.168309206738925 22.035768613442013 26-27 21.74345433322058 26.96206821139756 29.271523966591257 22.022953488790602 28-29 21.82709004566444 27.09613079959896 29.249628105210917 21.827151049525682 30-31 21.934349029794927 27.20324646246991 28.998122793809916 21.864281713925244 32-33 21.78625873861892 27.10380796734786 29.267668082335007 21.842265211698216 34-35 21.967653568063522 26.898479355579045 29.21404872816431 21.919818348193115 36-37 22.07838686897826 27.128132082745303 28.87352037031302 21.919960677963413 38-39 21.90481493742763 27.404181051777243 28.856610641650942 21.834393369144188 40-41 21.875339959112598 27.062950090607448 29.027171859067696 22.034538091212262 42-43 21.903201597059805 27.1740666395851 28.81669307170624 22.106038691648855 44-45 21.926792738901206 27.278580924846224 28.73738930362898 22.057237032623593 46-47 22.10044903650198 27.3777462132344 28.466636448976658 22.055168301286958 48-49 22.152350344299478 27.33884118867819 28.270310738180648 22.23849772884168 50-51 22.042711416350894 27.468237110998782 28.209985660271013 22.279065812379308 52-53 22.178036507611324 27.440885874558095 28.1540687512079 22.22700886662268 54-55 22.131570876160588 27.69926761562805 27.97626471909694 22.19289678911443 56-57 22.00612348446965 27.49575190515529 28.13826289402873 22.359861716346327 58-59 22.012902402590555 27.1370644080721 28.38701041530095 22.463022774036396 60-61 22.158204421786877 27.472636664046462 28.11398655300573 22.25517236116093 62-63 21.95850896763282 27.41845702103979 28.22099326757868 22.40204074374871 64-65 21.96047913761573 27.291806202656886 28.2466898708156 22.501024788911785 66-67 21.85507561098092 27.33194793644539 28.208493335897337 22.604483116676352 68-69 22.06255974126504 27.42140077749806 28.140064939936966 22.375974541299932 70-71 22.117201691877693 27.22652165903565 28.10401241458567 22.55226423450098 72-73 21.941965837929633 27.377342527153857 28.130653876325056 22.55003775859145 74-75 21.97967867987668 27.50725587149147 27.96508641776496 22.547979030866884 76-77 22.0699406659058 27.369032812174986 27.840832495100226 22.720194026818984 78-79 22.011249194999213 27.41777402089563 27.97707705453052 22.593899729574634 80-81 21.95451757501486 27.353717932959754 28.033679027497648 22.65808546452774 82-83 21.895868477902496 27.35118502073523 28.121180863623874 22.631765637738397 84-85 21.89539284184945 27.34775784726009 27.90782328735203 22.849026023538425 86-87 22.028622602996155 27.32645002100679 27.91491451267689 22.73001286332017 88-89 21.739597213906798 27.355078044342402 27.886124830078145 23.019199911672658 90-91 21.756125412432542 27.274364553462664 27.95758891127569 23.011921122829108 92-93 21.924544322997 27.27435902049994 27.905113409520194 22.89598324698287 94-95 21.66384389379786 27.162562742265127 27.941166154419555 23.232427209517457 96-97 21.697133619207396 27.106796211261326 28.012851980340475 23.183218189190804 98-99 21.619895916794434 27.243045539376155 27.891468721206397 23.245589822623014 100 21.663122605013413 26.931330603002873 27.865809991935887 23.539736800047823 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 58.0 1 59.5 2 208.0 3 402.0 4 290.0 5 186.5 6 462.5 7 1074.0 8 1906.5 9 2273.0 10 1830.0 11 1422.0 12 1417.5 13 1553.5 14 1795.5 15 2064.5 16 2274.5 17 2517.0 18 2781.0 19 3175.5 20 3737.0 21 4416.5 22 5596.5 23 7656.0 24 10399.5 25 13618.5 26 17883.5 27 23568.0 28 30955.0 29 39769.0 30 48219.5 31 56166.5 32 63862.5 33 71659.0 34 79359.0 35 85885.5 36 92459.5 37 99392.5 38 104963.0 39 107899.5 40 111515.0 41 115714.0 42 117637.0 43 119282.0 44 118889.0 45 117257.0 46 116931.5 47 114738.5 48 112848.0 49 110078.5 50 106219.0 51 103347.0 52 97145.0 53 91994.5 54 89325.0 55 83722.5 56 76217.0 57 68360.5 58 62002.0 59 58453.5 60 53577.5 61 46145.5 62 39842.0 63 34153.0 64 27794.5 65 22257.5 66 17543.0 67 13431.0 68 10104.5 69 7642.0 70 5523.5 71 4073.5 72 3182.5 73 2257.0 74 1607.5 75 1080.5 76 669.0 77 432.0 78 260.0 79 125.5 80 70.5 81 43.5 82 21.0 83 12.0 84 6.5 85 1.0 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.134976899549947E-4 2 4.269953799099894E-4 3 1.8299801996142403E-4 4 0.011010380867679012 5 5.794937298778428E-4 6 0.003598961059241339 7 5.48994059884272E-4 8 0.13630302520126733 9 0.0011894871297492562 10-11 0.0 12-13 1.37248514971068E-4 14-15 1.5249834996785335E-4 16-17 0.0 18-19 1.8299801996142403E-4 20-21 0.0 22-23 2.74497029942136E-4 24-25 3.507462049260627E-4 26-27 0.005520440268836291 28-29 0.007411419808437673 30-31 0.01366385215711966 32-33 0.01621057460158281 34-35 0.0245369845098276 36-37 0.03576086306746161 38-39 0.040229064721519714 40-41 0.051102197074227654 42-43 0.06719077299583619 44-45 0.10307363474327208 46-47 0.10981406181185119 48-49 0.10745033738734948 50-51 0.10850257600212765 52-53 0.10391237566809527 54-55 0.10978356214185762 56-57 0.09570796443982477 58-59 0.08608531855685322 60-61 0.08841854331136137 62-63 0.08721380634661532 64-65 0.0 66-67 1.5249834996785334E-5 68-69 6.0999339987141335E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 6.0999339987141335E-5 78-79 1.5249834996785334E-5 80-81 2.134976899549947E-4 82-83 1.5249834996785335E-4 84-85 0.0 86-87 6.557429048617695E-4 88-89 3.354963699292774E-4 90-91 1.8299801996142403E-4 92-93 9.454897698006907E-4 94-95 0.001006489109787832 96-97 2.2874752495178003E-4 98-99 3.0499669993570668E-5 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3278724.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.89030723018894 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7382271230697 41.21252168517817 2 14.984561806844996 16.749835261886435 3 4.828434009914456 8.095879807644362 4 2.1175096712513373 4.733930643565344 5 1.1856050265796605 3.313191459459677 6 0.7161779462120494 2.4016443267166303 7 0.44779434848825844 1.7519154599065743 8 0.3236460033396822 1.4470939648382075 9 0.24095430120613137 1.2120308930561552 >10 1.3128616189066875 13.008820368934964 >50 0.077194444987212 2.9198882188179023 >100 0.026160928901744207 2.3826553386234735 >500 5.454814373788791E-4 0.1898738151229259 >1k 2.181925737857954E-4 0.21487325672219906 >5k 1.090962868928977E-4 0.36584549952701323 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6658 0.2030668028171935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5342 0.16292923710565452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 3.049966999357067E-5 0.0 3.049966999357067E-5 0.0 12-13 0.0 6.099933998714134E-5 0.0 3.049966999357067E-5 0.0 14-15 0.0 6.099933998714134E-5 0.0 4.574950499035601E-5 0.0 16-17 0.0 6.099933998714134E-5 0.0 6.099933998714134E-5 0.0 18-19 0.0 6.099933998714134E-5 0.0 6.099933998714134E-5 0.0 20-21 0.0 6.099933998714134E-5 0.0 6.099933998714134E-5 0.0 22-23 0.0 6.099933998714134E-5 0.0 9.149900998071202E-5 0.0 24-25 0.0 6.099933998714134E-5 0.0 2.4399735994856534E-4 0.0 26-27 0.0 6.099933998714134E-5 0.0 5.032445548939161E-4 0.0 28-29 0.0 6.099933998714134E-5 0.0 0.0015859828396656748 0.0 30-31 0.0 6.099933998714134E-5 0.0 0.005977935318739851 0.0 32-33 0.0 6.099933998714134E-5 0.0 0.014868589121865703 0.0 34-35 0.0 6.099933998714134E-5 0.0 0.028425692434007863 0.0 36-37 0.0 6.099933998714134E-5 0.0 0.05195618783404764 0.0 38-39 0.0 6.099933998714134E-5 0.0 0.09168200800067344 0.0 40-41 0.0 6.099933998714134E-5 0.0 0.13889549715072083 0.0 42-43 0.0 6.099933998714134E-5 0.0 0.1868867278856043 0.0 44-45 0.0 6.099933998714134E-5 0.0 0.24178613387403147 0.0 46-47 0.0 6.099933998714134E-5 3.049966999357067E-5 0.31033414218458155 0.0 48-49 0.0 6.099933998714134E-5 3.049966999357067E-5 0.3865070679935243 0.0 50-51 0.0 6.099933998714134E-5 3.049966999357067E-5 0.4738276231851172 0.0 52-53 0.0 6.099933998714134E-5 3.049966999357067E-5 0.5622461664964785 0.0 54-55 0.0 7.624917498392668E-5 3.049966999357067E-5 0.6550261626169205 0.0 56-57 0.0 1.0674884497749734E-4 3.049966999357067E-5 0.7678139422531448 0.0 58-59 0.0 1.2199867997428268E-4 3.049966999357067E-5 0.8992522700904376 0.0 60-61 0.0 1.2199867997428268E-4 3.049966999357067E-5 1.0405572411706505 0.0 62-63 0.0 1.37248514971068E-4 3.049966999357067E-5 1.182899201030645 0.0 64-65 0.0 1.5249834996785335E-4 3.049966999357067E-5 1.3328813282240286 0.0 66-67 0.0 1.5249834996785335E-4 3.049966999357067E-5 1.508315430027047 0.0 68-69 0.0 2.134976899549947E-4 3.049966999357067E-5 1.7089575090797517 0.0 70-71 0.0 2.134976899549947E-4 3.049966999357067E-5 1.9243614284093447 0.0 72-73 0.0 2.134976899549947E-4 3.049966999357067E-5 2.152666708146218 0.0 74-75 0.0 2.134976899549947E-4 3.049966999357067E-5 2.3776932733587826 0.0 76-77 0.0 2.134976899549947E-4 3.049966999357067E-5 2.613516721749071 0.0 78-79 0.0 2.134976899549947E-4 3.049966999357067E-5 2.887083511756403 0.0 80-81 0.0 2.134976899549947E-4 3.049966999357067E-5 3.1860412770333824 0.0 82-83 0.0 2.134976899549947E-4 3.049966999357067E-5 3.501346865426916 0.0 84-85 0.0 2.134976899549947E-4 3.049966999357067E-5 3.807975297707279 0.0 86-87 0.0 2.134976899549947E-4 3.049966999357067E-5 4.131485297329083 0.0 88 0.0 2.134976899549947E-4 3.049966999357067E-5 4.399394398552608 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6150 0.0 37.734642 1 GTATCAA 10015 0.0 35.79002 1 TCAACGC 11480 0.0 30.484297 4 ATCAACG 11575 0.0 30.2752 3 CAACGCA 11850 0.0 29.49052 5 AACGCAG 12120 0.0 28.911165 6 TATCAAC 12360 0.0 28.466118 2 GTACATG 11120 0.0 25.938923 1 ACGCAGA 14015 0.0 24.934523 7 TACATGG 11235 0.0 24.701542 2 CGCAGAG 14550 0.0 23.92071 8 ACATGGG 11360 0.0 23.809008 3 GCAGAGT 15935 0.0 21.577824 9 CCGGTCG 355 0.0 21.170946 9 CATGGGG 7540 0.0 20.7739 4 AGAGTAC 13105 0.0 20.32301 10-11 GAGTACA 7965 0.0 19.876162 1 GAGTACT 8745 0.0 19.79337 12-13 TACGTCG 270 2.5784175E-8 19.162003 5 GTATAGA 1355 0.0 18.721596 1 >>END_MODULE