FastQCFastQC Report
Wed 25 May 2016
SRR1032965_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032965_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150161
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47850.037.031381
GGTATCA28950.034.5813181
TCAACGC49100.034.0783844
ATCAACG50400.033.479153
CAACGCA50800.032.9379655
AACGCAG53550.031.4220166
TATCAAC58700.028.9855122
GTACATG108250.028.9606591
TACATGG108400.026.88272
ACGCAGA62700.026.386737
ACATGGG112550.025.01453
CGCAGAG66000.024.9249678
CATGGGG77650.021.427544
GCAGAGT79050.020.3342329
GAGTACA80900.020.276241
AGTACAT80600.019.0687922
CATGGGA36250.017.3742984
GTATAGG13300.017.3162921
GTGGTAT19100.017.2256321
GTATAGA13200.016.7353341
Produced by FastQC (version 0.11.5)