##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032965_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3150161
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66604944953607	33.0	31.0	34.0	30.0	34.0
2	31.68041506449988	34.0	31.0	34.0	30.0	34.0
3	31.84421304180961	34.0	31.0	34.0	30.0	34.0
4	35.14871906546999	37.0	35.0	37.0	33.0	37.0
5	35.23911952436717	37.0	35.0	37.0	33.0	37.0
6	35.293621183171275	37.0	36.0	37.0	33.0	37.0
7	35.24418593208411	37.0	37.0	37.0	33.0	37.0
8	35.253274356453524	37.0	37.0	37.0	33.0	37.0
9	36.904679792556635	39.0	38.0	39.0	34.0	39.0
10-11	36.89698701114007	39.0	38.0	39.0	34.0	39.0
12-13	36.84144064382741	39.0	38.0	39.0	34.0	39.0
14-15	38.32736914081534	41.0	39.0	41.0	34.0	41.0
16-17	38.34682243225029	41.0	39.0	41.0	34.0	41.0
18-19	38.35399904957239	41.0	39.0	41.0	34.0	41.0
20-21	38.3976028526796	41.0	39.0	41.0	34.0	41.0
22-23	38.386989109445516	41.0	39.0	41.0	34.0	41.0
24-25	38.41698138603074	41.0	39.0	41.0	34.0	41.0
26-27	38.39689019069184	41.0	39.0	41.0	34.0	41.0
28-29	38.3726385730761	41.0	39.0	41.0	34.0	41.0
30-31	38.3252027436058	41.0	39.0	41.0	34.0	41.0
32-33	38.280994685668446	41.0	39.0	41.0	34.0	41.0
34-35	38.2073927332603	41.0	39.0	41.0	33.5	41.0
36-37	38.11591709122169	41.0	39.0	41.0	33.0	41.0
38-39	38.02219807178109	41.0	38.0	41.0	33.0	41.0
40-41	37.936239766792866	41.0	38.0	41.0	33.0	41.0
42-43	37.81646620601296	40.5	38.0	41.0	33.0	41.0
44-45	37.67896656710562	40.0	38.0	41.0	33.0	41.0
46-47	37.55687375978562	40.0	38.0	41.0	32.0	41.0
48-49	37.42312551644186	40.0	38.0	41.0	32.0	41.0
50-51	37.280142189557935	40.0	37.0	41.0	32.0	41.0
52-53	37.12257722700522	40.0	37.0	41.0	31.0	41.0
54-55	36.95208514739406	40.0	36.5	41.0	31.0	41.0
56-57	36.76482202020786	40.0	36.0	41.0	31.0	41.0
58-59	36.55333076626877	39.5	35.5	41.0	31.0	41.0
60-61	36.30422175247551	39.0	35.0	41.0	30.0	41.0
62-63	36.04614700645459	39.0	35.0	41.0	30.0	41.0
64-65	35.765057881168616	38.5	35.0	40.5	30.0	41.0
66-67	35.467425474444006	38.0	35.0	40.0	29.0	41.0
68-69	35.15305360583157	37.0	35.0	40.0	29.0	41.0
70-71	34.818396900983785	37.0	34.5	39.5	29.0	41.0
72-73	34.52431193199332	36.0	34.0	39.0	29.0	41.0
74-75	34.25043926326305	36.0	34.0	39.0	29.0	41.0
76-77	33.927215942296286	35.0	34.0	38.5	29.0	40.0
78-79	33.5613763867942	35.0	34.0	37.0	28.0	39.0
80-81	33.24495478167624	35.0	34.0	37.0	28.0	39.0
82-83	32.93213616700861	35.0	34.0	36.0	27.5	38.5
84-85	32.76822041794054	35.0	34.0	36.0	28.0	37.0
86-87	32.538762780695976	35.0	34.0	36.0	27.0	37.0
88-89	32.3052158286513	35.0	34.0	35.0	27.0	37.0
90-91	32.10907252042038	35.0	34.0	35.0	27.0	36.0
92-93	31.92021995066284	35.0	33.5	35.0	26.0	36.0
94-95	31.73839987860938	35.0	33.0	35.0	26.0	36.0
96-97	31.56615630121762	35.0	33.0	35.0	25.0	36.0
98-99	31.387435118395537	35.0	33.0	35.0	25.0	35.0
100	31.259259447374276	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3780019984153924
1101	2	0.37221983726623975
1101	3	0.409868406515578
1101	4	0.41524122533795804
1101	5	0.33016291251756513
1101	6	0.38115377825331365
1101	7	0.3032781375349529
1101	8	0.311399221861862
1101	9	0.39267777747118515
1101	10-11	0.3819520326513768
1101	12-13	0.15308257312201334
1101	14-15	0.21500983277418584
1101	16-17	0.28437924572995854
1101	18-19	0.3411479500813428
1101	20-21	0.2394306911724584
1101	22-23	0.2744615357122768
1101	24-25	0.24045637759342497
1101	26-27	0.19814772583197282
1101	28-29	0.2797256384271023
1101	30-31	0.22970601923788792
1101	32-33	0.22636416999502984
1101	34-35	0.25474415293367514
1101	36-37	0.20959335842350413
1101	38-39	0.25552684869868614
1101	40-41	0.21529641631371987
1101	42-43	0.22781992439173848
1101	44-45	0.19382634010102606
1101	46-47	0.2156415994953207
1101	48-49	0.20072922201937615
1101	50-51	0.22777416971119635
1101	52-53	0.19098231468640137
1101	54-55	0.22061610662906617
1101	56-57	0.20494197837221861
1101	58-59	0.14708832409191075
1101	60-61	0.2984285818509278
1101	62-63	0.2718814070970694
1101	64-65	0.24024347189472195
1101	66-67	0.2471055000267981
1101	68-69	0.20425672568175912
1101	70-71	0.2565849388720949
1101	72-73	0.1622389446081982
1101	74-75	0.1625026407199499
1101	76-77	0.16954501772500663
1101	78-79	0.2206595521036121
1101	80-81	0.14299768168057625
1101	82-83	0.11970204780939753
1101	84-85	0.09560445934116046
1101	86-87	0.10907222730752153
1101	88-89	0.17493413496816146
1101	90-91	0.13153988794519478
1101	92-93	0.1280917890179687
1101	94-95	0.12689468040442975
1101	96-97	0.09155683551776406
1101	98-99	0.08809756568167515
1101	100	0.10493285218934645
1102	1	0.4145774711190029
1102	2	0.38195268169208774
1102	3	0.4541833344647159
1102	4	0.4168497073572581
1102	5	0.41392119619436585
1102	6	0.39754390438822185
1102	7	0.3954250014484373
1102	8	0.38824384296901826
1102	9	0.4082722272845771
1102	10-11	0.41890295217420004
1102	12-13	0.495840776637813
1102	14-15	0.552098604591194
1102	16-17	0.5573719627222076
1102	18-19	0.5253230597765182
1102	20-21	0.4818686873086122
1102	22-23	0.5328815757838257
1102	24-25	0.5034019317029887
1102	26-27	0.5438985321441834
1102	28-29	0.5334771957449078
1102	30-31	0.48082474663942776
1102	32-33	0.5321819779185191
1102	34-35	0.5630903351651639
1102	36-37	0.5844495500324811
1102	38-39	0.573348262971642
1102	40-41	0.5471234467829618
1102	42-43	0.6048114662863853
1102	44-45	0.6372159346654769
1102	46-47	0.6370297977357282
1102	48-49	0.6602738896109486
1102	50-51	0.6571095588682354
1102	52-53	0.5996323026398613
1102	54-55	0.6558028011565327
1102	56-57	0.6720972390387843
1102	58-59	0.6277255234545578
1102	60-61	0.6969762122648149
1102	62-63	0.6517551064959974
1102	64-65	0.6489016886661929
1102	66-67	0.6364878505444409
1102	68-69	0.6461211340174344
1102	70-71	0.6518235208553023
1102	72-73	0.6386634369740918
1102	74-75	0.6509585606771751
1102	76-77	0.6631443967683239
1102	78-79	0.6962269039154734
1102	80-81	0.6613911611427881
1102	82-83	0.6204444887164087
1102	84-85	0.6059278194216944
1102	86-87	0.6633911028501771
1102	88-89	0.6245883230894691
1102	90-91	0.5870973293492909
1102	92-93	0.5837268723988451
1102	94-95	0.5674988480600511
1102	96-97	0.5535443984710184
1102	98-99	0.5449075787313511
1102	100	0.533809554714999
1103	1	0.18230191615599978
1103	2	0.24535688141211054
1103	3	0.3230699420242118
1103	4	0.28793478168563524
1103	5	0.22512178282192963
1103	6	0.25980434035915323
1103	7	0.2630202817630831
1103	8	0.22639137979989954
1103	9	0.2992645611289859
1103	10-11	0.3246405163365935
1103	12-13	0.3009368369004619
1103	14-15	0.3457097505147999
1103	16-17	0.3738682229715238
1103	18-19	0.4289320191792214
1103	20-21	0.3956795532508792
1103	22-23	0.38504996455790064
1103	24-25	0.39817516015443033
1103	26-27	0.41494928876041115
1103	28-29	0.45551138013261294
1103	30-31	0.45218841572815904
1103	32-33	0.38899888345815015
1103	34-35	0.3462615370850344
1103	36-37	0.2951801146615125
1103	38-39	0.32093855695205065
1103	40-41	0.40507579662630633
1103	42-43	0.3717282051704629
1103	44-45	0.3473588051407859
1103	46-47	0.2940666886096679
1103	48-49	0.31464389161081385
1103	50-51	0.33948209403255447
1103	52-53	0.3166212233784833
1103	54-55	0.31813669889668716
1103	56-57	0.2546932259729928
1103	58-59	0.29144044912618483
1103	60-61	0.2532862182644138
1103	62-63	0.26518543780724
1103	64-65	0.26030330189198025
1103	66-67	0.2313520862620635
1103	68-69	0.27180536840180736
1103	70-71	0.295002295603652
1103	72-73	0.30297165642613066
1103	74-75	0.28640689078849846
1103	76-77	0.241103092855262
1103	78-79	0.23868242021438846
1103	80-81	0.22091580616645246
1103	82-83	0.27542639658921786
1103	84-85	0.31534761810178225
1103	86-87	0.3307232007103238
1103	88-89	0.2967096216695566
1103	90-91	0.3510914097439297
1103	92-93	0.3595413914309127
1103	94-95	0.3042814092226358
1103	96-97	0.32845521774973463
1103	98-99	0.3020554488733431
1103	100	0.31061472770346654
1104	1	-0.08599252311285
1104	2	-0.030492185374487946
1104	3	0.04083562675549146
1104	4	-0.03974001673384464
1104	5	0.05032400582940966
1104	6	-0.01519566606572198
1104	7	0.0022156558515575853
1104	8	0.03580367058983569
1104	9	0.06523507094298964
1104	10-11	0.017740943590922598
1104	12-13	0.02108576555220054
1104	14-15	0.01613786577709675
1104	16-17	0.07227672950883601
1104	18-19	0.12372941067911114
1104	20-21	0.07007618661526038
1104	22-23	0.05235337938010076
1104	24-25	0.11918859457249908
1104	26-27	0.10903745092430484
1104	28-29	0.14438223367753977
1104	30-31	0.12670717315691604
1104	32-33	0.13048940355195526
1104	34-35	0.15833372700439696
1104	36-37	0.2094633617944055
1104	38-39	0.17818074269523976
1104	40-41	0.19892806300170918
1104	42-43	0.26476775028915966
1104	44-45	0.15480482653562433
1104	46-47	0.19896110860388205
1104	48-49	0.12085296996218631
1104	50-51	0.21182680154002043
1104	52-53	0.20611002160521252
1104	54-55	0.13036699219232872
1104	56-57	0.13728535549851273
1104	58-59	0.07009217581190796
1104	60-61	0.17355267076851533
1104	62-63	0.15996286078902955
1104	64-65	0.16787044507037052
1104	66-67	0.14927641405929393
1104	68-69	0.12359447823507708
1104	70-71	0.11084285585293685
1104	72-73	0.1462865589286153
1104	74-75	0.1411840567752023
1104	76-77	0.0991122290305313
1104	78-79	0.093894576081901
1104	80-81	0.03708989463701329
1104	82-83	0.11660875387664049
1104	84-85	0.06493078492371751
1104	86-87	0.07343915046778449
1104	88-89	0.07451275058472362
1104	90-91	0.09256075344273285
1104	92-93	-0.009721174022523371
1104	94-95	0.057405480024780076
1104	96-97	-0.04199834002596958
1104	98-99	0.05826346069511246
1104	100	0.05202570781847271
1105	1	0.1110052243937929
1105	2	0.14029954487154583
1105	3	0.1548512671936848
1105	4	0.15985320972600192
1105	5	0.1832136887292677
1105	6	0.19577769951694535
1105	7	0.21968002997463998
1105	8	0.18943648304565386
1105	9	0.23158357646746452
1105	10-11	0.25960627185813223
1105	12-13	0.3178855107938148
1105	14-15	0.32336840535219835
1105	16-17	0.30608169958894393
1105	18-19	0.3135757978802971
1105	20-21	0.31692709940688957
1105	22-23	0.31001613243769555
1105	24-25	0.3440976492987815
1105	26-27	0.34889367037922625
1105	28-29	0.33909412356456414
1105	30-31	0.3180004678352404
1105	32-33	0.32011586199677566
1105	34-35	0.39437739725810417
1105	36-37	0.3922713895357717
1105	38-39	0.411973763599768
1105	40-41	0.44612496097606424
1105	42-43	0.4194165622976982
1105	44-45	0.34937263788577866
1105	46-47	0.3894209605786756
1105	48-49	0.3869066013043607
1105	50-51	0.3688384277928236
1105	52-53	0.35918756619813763
1105	54-55	0.37662506851962263
1105	56-57	0.33441437182909795
1105	58-59	0.3659706543681338
1105	60-61	0.3524374350336501
1105	62-63	0.3519898987737875
1105	64-65	0.32325223819512416
1105	66-67	0.3456427238553914
1105	68-69	0.3252422744653458
1105	70-71	0.30836730138291557
1105	72-73	0.3632130234562112
1105	74-75	0.3806084340080176
1105	76-77	0.27003557231544306
1105	78-79	0.2753877027735072
1105	80-81	0.33439644719609873
1105	82-83	0.3330262966235793
1105	84-85	0.2602612290480266
1105	86-87	0.16641290737365466
1105	88-89	0.25657161910793747
1105	90-91	0.2940695360130263
1105	92-93	0.25483895789244215
1105	94-95	0.29373418483102043
1105	96-97	0.2015157531906766
1105	98-99	0.21939238930006866
1105	100	0.2107678500676542
1106	1	-0.023389145723296423
1106	2	0.03061463542280407
1106	3	0.018599353788918194
1106	4	0.026571298110390273
1106	5	0.06900260787922008
1106	6	-0.01406823412518321
1106	7	0.06466184020464283
1106	8	0.08266273197713048
1106	9	0.04100329298536565
1106	10-11	0.11688436660397628
1106	12-13	0.12336311650321363
1106	14-15	0.1619609346248012
1106	16-17	0.12308625200131473
1106	18-19	0.10392667158564706
1106	20-21	0.11486121864431453
1106	22-23	0.11237463988258156
1106	24-25	0.07327920904671004
1106	26-27	0.13852331473442803
1106	28-29	0.11170450465515103
1106	30-31	0.13538352649973717
1106	32-33	0.10952325320605638
1106	34-35	0.11606994044637986
1106	36-37	0.1031458900625779
1106	38-39	0.1521471131017833
1106	40-41	0.13347243528176733
1106	42-43	0.13537068034234778
1106	44-45	0.08799379368165461
1106	46-47	0.03439243345305698
1106	48-49	0.0845830818323634
1106	50-51	0.11354351496456871
1106	52-53	0.154568358596201
1106	54-55	0.15206549950783455
1106	56-57	0.13881729014411093
1106	58-59	0.104902328423357
1106	60-61	0.15134763919642324
1106	62-63	0.1640046891441429
1106	64-65	0.18361086491719192
1106	66-67	0.20228745677390236
1106	68-69	0.19630284739339743
1106	70-71	0.20063774250968436
1106	72-73	0.1448980124230701
1106	74-75	0.10754241408872645
1106	76-77	0.08446015931820483
1106	78-79	0.1660339862953677
1106	80-81	0.1229533921099204
1106	82-83	0.11895397489326598
1106	84-85	0.0994441803630508
1106	86-87	0.10271770032835548
1106	88-89	0.1359346789652065
1106	90-91	0.0923161614628043
1106	92-93	0.09894368325673142
1106	94-95	0.14439080570850393
1106	96-97	0.15274490453354161
1106	98-99	0.16754299661971217
1106	100	0.15027125940705943
1107	1	0.14293297205662014
1107	2	0.17030263710569216
1107	3	0.1602459254610622
1107	4	0.13452360156141197
1107	5	0.16226463996478202
1107	6	0.09721552048338111
1107	7	0.11590972275687506
1107	8	0.12984976489306632
1107	9	0.12781942655341538
1107	10-11	0.10616515401775217
1107	12-13	0.09220391764581137
1107	14-15	0.11399877742991293
1107	16-17	0.17257795382038665
1107	18-19	0.13140820965541167
1107	20-21	0.15811350770222532
1107	22-23	0.09794023370904625
1107	24-25	0.1514554914173729
1107	26-27	0.15946440263203954
1107	28-29	0.1341689370684378
1107	30-31	0.10632671800559024
1107	32-33	0.14609370747471928
1107	34-35	0.1859518062361687
1107	36-37	0.21479999043790343
1107	38-39	0.14895926088579614
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2216	40-41	-0.3133606816661967
2216	42-43	-0.23409619453848762
2216	44-45	-0.22083028047649123
2216	46-47	-0.16326515713372913
2216	48-49	-0.14304255030041446
2216	50-51	-0.19196568670962222
2216	52-53	-0.20708685326474807
2216	54-55	-0.21439019899320755
2216	56-57	-0.2436830049268437
2216	58-59	-0.19406156397107566
2216	60-61	-0.1694247203725041
2216	62-63	-0.12036227017238588
2216	64-65	-0.118748068469408
2216	66-67	-0.0879865506528077
2216	68-69	-0.06601214311893244
2216	70-71	-0.12332183730927682
2216	72-73	-0.15599627954282624
2216	74-75	-0.15612990280840222
2216	76-77	-0.1769136910399638
2216	78-79	-0.07578510343343936
2216	80-81	-0.07362496511660055
2216	82-83	-0.0895342631245839
2216	84-85	-0.12894954415870075
2216	86-87	-0.09976489048541026
2216	88-89	-0.11408756484511429
2216	90-91	-0.09148671298223832
2216	92-93	-0.180299210072846
2216	94-95	-0.16781405524498538
2216	96-97	-0.19586581062765873
2216	98-99	-0.1884489411339345
2216	100	-0.23905570644642538
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44954.0
3	6483.0
4	4431.0
5	3780.0
6	3084.0
7	3420.0
8	3700.0
9	4241.0
10	4489.0
11	4709.0
12	5202.0
13	5820.0
14	6144.0
15	6306.0
16	6095.0
17	6023.0
18	5936.0
19	6021.0
20	6140.0
21	6394.0
22	7446.0
23	10357.0
24	15891.0
25	20946.0
26	22678.0
27	20165.0
28	20984.0
29	26869.0
30	32629.0
31	39453.0
32	50783.0
33	68730.0
34	99133.0
35	157076.0
36	303553.0
37	637284.0
38	1022181.0
39	449877.0
40	754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.79998913530014	19.34189780892043	12.960189134728314	23.897923921051117
2	15.505750836479528	20.00107379445922	40.178052912016206	24.315122457045042
3	18.32815651619745	25.41240599843631	29.724492937194835	26.534944548171403
4	12.375179936911284	16.374250984311693	38.49107471619132	32.759494362585706
5	12.91932987913921	38.26276634375066	35.39721908674699	13.42068469036313
6	31.125873259437874	37.719788162112536	17.746950938624835	13.40738763982476
7	27.950827744614337	32.50343900804077	22.1202080242462	17.4255252230987
8	25.6648467238806	36.514579768581555	20.01545246429318	17.80512104324466
9	25.575954003737287	17.001627202298543	20.54954452949328	36.87287426447089
10-11	24.18465643974442	26.966193972654963	28.499898736791106	20.34925085080951
12-13	24.632109012926122	24.37845353231419	28.563268608748377	22.426168846011315
14-15	22.026380567184894	26.251969822189047	25.41497508028996	26.306674530336092
16-17	21.998511679030955	29.613822800854873	26.023903673706673	22.363761846407503
18-19	21.960732364325665	28.660666081189458	27.795983270536695	21.58261828394819
20-21	22.897232556813428	27.97341185840137	27.371163258197328	21.75819232658787
22-23	22.77541802468732	27.66591254460839	27.329901212287076	22.228768218417212
24-25	22.43045847538299	27.76323946764953	27.371173673408546	22.435128383558936
26-27	22.258370512313856	27.777114182568596	27.439834857978624	22.524680447138923
28-29	22.329150411425307	27.865420102358385	27.50269301474384	22.302736471472468
30-31	22.38359390375295	28.15885543118849	27.168816497715632	22.28873416734293
32-33	22.19929647063084	27.924994988631894	27.60612584553931	22.26958269519795
34-35	22.42446373288703	27.681184672586834	27.560836184535198	22.33351540999094
36-37	22.557228593219957	27.98396649590395	27.15273315114549	22.3060717597306
38-39	22.36666261114692	28.131737470429385	27.28941766654288	22.21218225188081
40-41	22.239376548257344	27.81597792047636	27.569305065545407	22.375340465720893
42-43	22.239296157125665	27.800954841365954	27.510429678614678	22.4493193228937
44-45	22.25067672094254	27.981833173932014	27.503676872957545	22.2638132321679
46-47	22.298272615774362	27.94688876883347	27.40306526787457	22.351773347517593
48-49	22.203163152502864	27.801848722702555	27.564614667812755	22.43037345698183
50-51	22.165633912360946	27.855343123463953	27.471862892632366	22.507160071542735
52-53	22.31677548879409	27.777601270345176	27.473994365670944	22.431628875189794
54-55	22.308594369292877	28.08444885148202	27.313199624716955	22.293757154508146
56-57	22.160830713842287	27.89584814640429	27.435094126939042	22.508227012814377
58-59	22.12714787004046	27.489781881086987	27.710045288545697	22.673024960326853
60-61	22.37963930909168	27.958938301182133	27.28227551094808	22.379146878778112
62-63	22.12206774128215	27.908566345393965	27.383855351831333	22.585510561492548
64-65	22.1800091591951	27.592988625029445	27.553864279663298	22.673137936112152
66-67	22.033068799878006	27.67644741523916	27.489547857469628	22.800935927413207
68-69	22.341695965902648	27.776581134183036	27.36597882957455	22.515744070339768
70-71	22.251174132876244	27.579427009495834	27.481896981286784	22.687501876341138
72-73	21.978561522407343	27.700344198072813	27.612821406009974	22.70827287350987
74-75	22.15212493423912	27.735335005629384	27.46562008381812	22.646919976313377
76-77	22.194737204098576	27.6651718014833	27.36829548358833	22.77179551082979
78-79	22.096289876693504	27.692750985937327	27.489924600494447	22.721034536874722
80-81	22.08173641432805	27.624083148226276	27.559465112614557	22.734715324831118
82-83	22.08281199442966	27.56591267175592	27.62301705236877	22.72825828144565
84-85	22.030407539046664	27.577775645758333	27.50621709577441	22.885599719420593
86-87	22.167924075147152	27.57738558154666	27.526158416948775	22.728531926357416
88-89	21.91652161979368	27.530288296742555	27.557243348042924	22.995946735420844
90-91	21.920398766988885	27.48816791874338	27.565870427730708	23.025562886537024
92-93	22.151393878093007	27.503916887024655	27.531331919484302	22.813357315398036
94-95	21.9283014072874	27.36767368722005	27.549906286730835	23.154118618761714
96-97	22.0204468509737	27.353004009793775	27.583076748358238	23.043472390874285
98-99	21.94866537862611	27.412074077791974	27.55683568969362	23.0824248538883
100	22.07552761930659	27.23458136870184	27.511331938356285	23.17855907363528
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2899.0
1	1847.0
2	729.5
3	617.0
4	505.0
5	399.0
6	366.0
7	350.5
8	370.5
9	387.5
10	332.0
11	307.5
12	343.5
13	371.5
14	395.0
15	449.5
16	548.5
17	716.0
18	941.5
19	1265.0
20	1772.5
21	2516.0
22	3715.0
23	5541.0
24	8039.5
25	11448.0
26	15994.0
27	21293.0
28	28192.5
29	36763.5
30	45060.0
31	53242.5
32	61188.5
33	69063.0
34	77027.0
35	83693.0
36	90767.0
37	97928.5
38	103240.5
39	105634.5
40	108442.5
41	112386.0
42	113744.5
43	114444.0
44	114209.5
45	113588.0
46	113140.5
47	110257.0
48	106817.5
49	104160.5
50	101663.5
51	98905.0
52	93095.0
53	88297.0
54	86033.0
55	81149.5
56	74322.5
57	67170.0
58	60624.5
59	56883.5
60	52266.0
61	45501.5
62	39812.0
63	34391.5
64	28267.0
65	22376.5
66	17943.5
67	14170.5
68	10632.0
69	7930.5
70	5906.5
71	4360.0
72	3420.5
73	2593.0
74	1853.0
75	1305.0
76	912.0
77	652.5
78	443.5
79	285.0
80	211.5
81	165.5
82	123.5
83	107.5
84	86.5
85	69.0
86	55.5
87	41.5
88	35.0
89	26.0
90	19.5
91	14.5
92	10.0
93	7.5
94	5.0
95	3.5
96	2.5
97	2.5
98	3.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07466285056541554
2	0.07761508062603784
3	0.07853566849440394
4	0.08031335541262812
5	0.07904357904246799
6	0.07844043526664192
7	0.07780554708156186
8	0.07755159180752984
9	0.07704368125946578
10-11	0.07693257582707677
12-13	0.07578977709393266
14-15	0.07464697836078854
16-17	0.07359941285540643
18-19	0.07305975789808838
20-21	0.07274231380554835
22-23	0.07461523395153455
24-25	0.07452000072377253
26-27	0.07380575151555746
28-29	0.06990118917731507
30-31	0.07477395599780456
32-33	0.07358354065077943
34-35	0.07045671633926012
36-37	0.06755210289251883
38-39	0.06945676744775903
40-41	0.07196457577882527
42-43	0.07659925952990974
44-45	0.07763095283066485
46-47	0.08064667170979514
48-49	0.08369413499817946
50-51	0.08480518932206957
52-53	0.08475757270818857
54-55	0.08475757270818857
56-57	0.08263069728817035
58-59	0.08145615414577223
60-61	0.07959910620441304
62-63	0.0801070167524771
64-65	0.07950387297665103
66-67	0.07875787935918196
68-69	0.07834520203887992
70-71	0.07658338732528275
72-73	0.07648815409752073
74-75	0.07596437134482968
76-77	0.07572628827542466
78-79	0.0760278601633377
80-81	0.0772341477149898
82-83	0.07671036496229876
84-85	0.07583739370781367
86-87	0.07672623716692575
88-89	0.07390098474331946
90-91	0.07166300389091225
92-93	0.07152015404926923
94-95	0.07177410932330125
96-97	0.0712185821613562
98-99	0.07069479940866515
100	0.06983770035880706
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3150161.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.43130565162909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12489225951488	46.60757937483705
2	13.44026243454158	16.244252144397812
3	4.0373776739525224	7.3195201273706445
4	1.7832818777671007	4.3106420887341885
5	0.9623454172168431	2.907789502513778
6	0.5881925926711115	2.132714780983926
7	0.36961494846319076	1.5635419746793147
8	0.2797830672523308	1.352612484262072
9	0.20869329518394436	1.1350447477835934
>10	1.1307768653612684	12.142129946866707
>50	0.05772673959865539	2.410368003638834
>100	0.016580972475018982	1.6304462137935016
>500	3.669991124384383E-4	0.1439050654327501
>1k	1.0485688917981759E-4	0.09945354470576298
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.174440925400321E-5	0.0
4	0.0	0.0	0.0	3.174440925400321E-5	0.0
5	0.0	0.0	0.0	3.174440925400321E-5	0.0
6	0.0	0.0	0.0	3.174440925400321E-5	0.0
7	0.0	0.0	0.0	3.174440925400321E-5	0.0
8	0.0	0.0	0.0	3.174440925400321E-5	0.0
9	0.0	0.0	0.0	9.523322776200963E-5	0.0
10-11	0.0	0.0	0.0	9.523322776200963E-5	0.0
12-13	0.0	0.0	0.0	1.2697763701601284E-4	0.0
14-15	0.0	9.523322776200963E-5	0.0	1.9046645552401925E-4	0.0
16-17	0.0	2.2221086477802247E-4	0.0	1.9046645552401925E-4	0.0
18-19	0.0	2.698274786590273E-4	0.0	2.2221086477802247E-4	0.0
20-21	0.0	3.1744409254003207E-4	0.0	2.539552740320257E-4	0.0
22-23	0.0	3.1744409254003207E-4	0.0	3.6506070642103685E-4	0.0
24-25	0.0	3.1744409254003207E-4	0.0	4.920383434370497E-4	0.0
26-27	0.0	3.1744409254003207E-4	0.0	7.777380267230787E-4	0.0
28-29	0.0	3.650607064210369E-4	0.0	0.0016665814858351684	0.0
30-31	0.0	3.809329110480385E-4	0.0	0.006475859487816655	0.0
32-33	0.0	4.126773203020417E-4	0.0	0.015697610376104586	0.0
34-35	0.0	4.2854952492904334E-4	0.0	0.029633406038611997	0.0
36-37	0.0	5.396549573180546E-4	0.0	0.05385439029941644	0.0
38-39	0.0	7.142492082150723E-4	0.0	0.09762993066068687	0.0
40-41	0.0	7.61865822096077E-4	0.0	0.15067483852412622	0.0
42-43	0.0	9.523322776200963E-4	0.0	0.20181508183232538	0.0
44-45	0.0	9.682044822470978E-4	0.0	0.2622881814612015	0.0
46-47	0.0	9.840766868740994E-4	0.0	0.336332016046164	0.0
48-49	0.0	9.840766868740994E-4	0.0	0.4236608859039268	0.0
50-51	0.0	9.840766868740994E-4	0.0	0.5199258069666916	0.0
52-53	0.0	9.840766868740994E-4	0.0	0.613889258358541	0.0
54-55	0.0	9.99948891501101E-4	0.0	0.7166459111137494	0.0
56-57	0.0	0.0010475655053821058	0.0	0.8417982445976571	0.0
58-59	0.0	0.0010793099146361091	0.0	0.9836163929399164	0.0
60-61	0.0	0.0011269265285171139	0.0	1.1365292123164499	0.0
62-63	0.0	0.0011427987331441155	0.0	1.2925053671859947	0.0
64-65	0.0	0.0011427987331441155	0.0	1.4585127553798043	0.0
66-67	0.0	0.0012380319609061252	0.0	1.6420430574818239	0.0
68-69	0.0	0.0012539041655331268	0.0	1.8532386122487075	0.0
70-71	0.0	0.001365009597922138	0.0	2.0859410042851776	0.0
72-73	0.0	0.0013967540071761412	0.0	2.328595268622778	0.0
74-75	0.0	0.0013967540071761412	0.0	2.568900446675583	0.0
76-77	0.0	0.0014602428256841476	0.0	2.8242366025101573	0.0
78-79	0.0	0.0014919872349381507	0.0	3.113126598926214	0.0
80-81	0.0	0.0014919872349381507	0.0	3.434697464669266	0.0
82-83	0.0	0.0015078594395651524	0.0	3.76848992797511	0.0
84-85	0.0	0.0015396038488191557	0.0	4.097473113278973	0.0
86-87	0.0	0.001571348258073159	0.0	4.442487225256106	0.0
88	0.0	0.0015872204627001604	0.0	4.722806231173581	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4785	0.0	37.03138	1
GGTATCA	2895	0.0	34.581318	1
TCAACGC	4910	0.0	34.078384	4
ATCAACG	5040	0.0	33.47915	3
CAACGCA	5080	0.0	32.937965	5
AACGCAG	5355	0.0	31.422016	6
TATCAAC	5870	0.0	28.985512	2
GTACATG	10825	0.0	28.960659	1
TACATGG	10840	0.0	26.8827	2
ACGCAGA	6270	0.0	26.38673	7
ACATGGG	11255	0.0	25.0145	3
CGCAGAG	6600	0.0	24.924967	8
CATGGGG	7765	0.0	21.42754	4
GCAGAGT	7905	0.0	20.334232	9
GAGTACA	8090	0.0	20.27624	1
AGTACAT	8060	0.0	19.068792	2
CATGGGA	3625	0.0	17.374298	4
GTATAGG	1330	0.0	17.316292	1
GTGGTAT	1910	0.0	17.225632	1
GTATAGA	1320	0.0	16.735334	1
>>END_MODULE