FastQCFastQC Report
Wed 25 May 2016
SRR1032965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150161
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72070.2287819574936011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59690.18948237883714517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32810.10415340676238453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45900.055.8794631
GTATCAA87400.043.858221
TCAACGC104400.036.3600774
ATCAACG104950.036.124713
CAACGCA106900.035.683495
AACGCAG111000.034.4507456
TATCAAC111350.034.2179072
ACGCAGA129800.029.279317
CGCAGAG133750.028.2739458
GTACATG103450.027.29081
TACATGG105050.025.4337832
GTGGTAT20900.025.1735231
GCAGAGT150400.024.8601079
ACATGGG106500.024.2479213
CAGAGTA144000.021.08841110-11
GAGTACT95400.020.9993112-13
CATGGGG72000.020.9059184
TACACCG4850.020.3658085
AGAGTAC137600.020.20882610-11
TGGTATC22900.019.7194332
Produced by FastQC (version 0.11.5)