FastQCFastQC Report
Wed 25 May 2016
SRR1032964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3522413
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112500.029.0363621
GTATCAA58900.028.887051
TCAACGC57000.028.6130774
ATCAACG58100.028.2331453
CAACGCA58650.027.8878485
TACATGG115000.027.3016282
GGTATCA40400.027.223531
AACGCAG61550.026.7266036
ACATGGG117300.025.764573
TATCAAC66550.025.2839492
CATGGGG79550.023.3381754
ACGCAGA74850.021.537737
CGCAGAG76650.020.7863858
GAGTACA77450.020.329811
GTATAGG14800.020.0073131
GTATAGA14500.017.8280831
TACACCG6400.017.625245
GCAGAGT90300.017.4358189
AGTACAT84200.017.1928772
GTATTAC14050.017.0609721
Produced by FastQC (version 0.11.5)