##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1032964_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3522413
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.763848532241962	33.0	31.0	34.0	30.0	34.0
2	31.79589616549791	34.0	31.0	34.0	30.0	34.0
3	31.85944947398275	34.0	31.0	34.0	30.0	34.0
4	35.19934800376901	37.0	35.0	37.0	33.0	37.0
5	35.24522394165591	37.0	35.0	37.0	33.0	37.0
6	35.31860034584247	37.0	36.0	37.0	33.0	37.0
7	35.231117986448496	37.0	36.0	37.0	33.0	37.0
8	35.23662387119285	37.0	36.0	37.0	33.0	37.0
9	36.890064850430655	39.0	38.0	39.0	34.0	39.0
10-11	36.89124813018803	39.0	38.0	39.0	34.0	39.0
12-13	36.86213953332559	39.0	38.0	39.0	34.0	39.0
14-15	38.35965316957438	41.0	39.0	41.0	34.0	41.0
16-17	38.40182468665656	41.0	39.0	41.0	34.0	41.0
18-19	38.420977182403085	41.0	39.0	41.0	34.0	41.0
20-21	38.457800235236476	41.0	39.0	41.0	34.0	41.0
22-23	38.46018723528445	41.0	39.0	41.0	34.0	41.0
24-25	38.50038155662042	41.0	39.0	41.0	34.0	41.0
26-27	38.48093423457159	41.0	39.0	41.0	34.0	41.0
28-29	38.462259678237615	41.0	39.0	41.0	34.0	41.0
30-31	38.42362522509427	41.0	39.0	41.0	34.0	41.0
32-33	38.36935646103963	41.0	39.0	41.0	34.0	41.0
34-35	38.298212333420295	41.0	39.0	41.0	34.0	41.0
36-37	38.21553875141842	41.0	39.0	41.0	33.0	41.0
38-39	38.1284921728372	41.0	39.0	41.0	33.0	41.0
40-41	38.04522141497888	41.0	38.0	41.0	33.0	41.0
42-43	37.93817561995144	40.5	38.0	41.0	33.0	41.0
44-45	37.821820439567986	40.0	38.0	41.0	33.0	41.0
46-47	37.704276301501274	40.0	38.0	41.0	33.0	41.0
48-49	37.57780064404714	40.0	38.0	41.0	32.0	41.0
50-51	37.44495733464531	40.0	37.5	41.0	32.0	41.0
52-53	37.295120560820095	40.0	37.0	41.0	32.0	41.0
54-55	37.13315020129667	40.0	37.0	41.0	31.0	41.0
56-57	36.95202748797486	40.0	36.0	41.0	31.0	41.0
58-59	36.74785849927308	40.0	36.0	41.0	31.0	41.0
60-61	36.50597289414955	39.0	35.0	41.0	31.0	41.0
62-63	36.25700748322244	39.0	35.0	41.0	30.0	41.0
64-65	35.97880586404831	39.0	35.0	41.0	30.0	41.0
66-67	35.6835875009546	38.0	35.0	40.0	30.0	41.0
68-69	35.371902159116495	37.0	35.0	40.0	29.5	41.0
70-71	35.03686861818872	37.0	35.0	40.0	29.0	41.0
72-73	34.74496289901269	36.0	34.0	39.0	29.0	41.0
74-75	34.467307780206355	36.0	34.0	39.0	29.0	41.0
76-77	34.14112626770342	35.5	34.0	38.5	29.0	40.0
78-79	33.773560198647914	35.0	34.0	37.0	29.0	39.5
80-81	33.44642195563098	35.0	34.0	37.0	28.5	39.0
82-83	33.12714111036951	35.0	34.0	36.5	28.0	39.0
84-85	32.95072355229213	35.0	34.0	36.0	28.0	37.5
86-87	32.71321335686645	35.0	34.0	36.0	28.0	37.0
88-89	32.474810023696826	35.0	34.0	35.5	27.5	37.0
90-91	32.27032094192248	35.0	34.0	35.0	27.0	36.0
92-93	32.07546148052486	35.0	34.0	35.0	27.0	36.0
94-95	31.89619956546833	35.0	33.5	35.0	26.5	36.0
96-97	31.722208327075787	35.0	33.0	35.0	26.0	36.0
98-99	31.545275639171216	35.0	33.0	35.0	25.0	35.5
100	31.41534851251117	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18304136975335084
1101	2	0.25634837247933717
1101	3	0.2629789158771203
1101	4	0.20657348602705383
1101	5	0.13452304697212725
1101	6	0.21238029399298597
1101	7	0.164664616405652
1101	8	0.08042352581005474
1101	9	0.16530830672663654
1101	10-11	0.18223079762537253
1101	12-13	-0.026445160506192167
1101	14-15	-0.03189361097194876
1101	16-17	0.044117244424427327
1101	18-19	0.07459343544473285
1101	20-21	0.08200459916648128
1101	22-23	0.06867313504375261
1101	24-25	0.10233877585012863
1101	26-27	0.11975095293221472
1101	28-29	0.07832235078135596
1101	30-31	0.15230528046137692
1101	32-33	0.2051627900591626
1101	34-35	0.17614206415930056
1101	36-37	0.14201088046102228
1101	38-39	0.11924307395873512
1101	40-41	0.12161283281365343
1101	42-43	0.12369426524504945
1101	44-45	0.11540945766571298
1101	46-47	0.11666653509994518
1101	48-49	0.12441886257251866
1101	50-51	0.10668419509409688
1101	52-53	0.0972515260709983
1101	54-55	0.12218374067203541
1101	56-57	0.0867722958006496
1101	58-59	0.03002482645793947
1101	60-61	0.07403466141654746
1101	62-63	0.07645373490258578
1101	64-65	0.00931499683747461
1101	66-67	-0.01785688459334267
1101	68-69	0.04111394233007104
1101	70-71	0.0766400843028876
1101	72-73	0.05019710175142933
1101	74-75	0.07740247872029471
1101	76-77	0.07913702390486321
1101	78-79	0.025835425180041227
1101	80-81	0.0700564093268099
1101	82-83	-0.0031620321352576752
1101	84-85	-0.017233144964663438
1101	86-87	0.06132679800979446
1101	88-89	-0.014836520489261318
1101	90-91	-0.060848471093635226
1101	92-93	-0.012548540231605898
1101	94-95	0.08132229846534855
1101	96-97	0.013949191073745482
1101	98-99	-0.026472523402951254
1101	100	0.0421341319472468
1102	1	0.2662800179763458
1102	2	0.23546925086427706
1102	3	0.2523434217409388
1102	4	0.30097446290326246
1102	5	0.23129299329528408
1102	6	0.22284921019839743
1102	7	0.20883388655009583
1102	8	0.24411583212860677
1102	9	0.27141822539097404
1102	10-11	0.2516487012927868
1102	12-13	0.27280142148745057
1102	14-15	0.28916988040057845
1102	16-17	0.240509544157554
1102	18-19	0.2835626145093002
1102	20-21	0.271572784612232
1102	22-23	0.24826758408311633
1102	24-25	0.3111420690123339
1102	26-27	0.32269441756119477
1102	28-29	0.3162965381223799
1102	30-31	0.34285938312936537
1102	32-33	0.35162082132767836
1102	34-35	0.3408284357763094
1102	36-37	0.2968824143196258
1102	38-39	0.348316637692875
1102	40-41	0.3666234532171089
1102	42-43	0.3759518739297789
1102	44-45	0.3122228231895221
1102	46-47	0.35073946819531443
1102	48-49	0.32281346832282765
1102	50-51	0.32019176331748866
1102	52-53	0.3102024132625587
1102	54-55	0.30157486730542615
1102	56-57	0.38444174509115925
1102	58-59	0.38485970513531953
1102	60-61	0.43603978019429235
1102	62-63	0.3421974142836035
1102	64-65	0.3957203446542934
1102	66-67	0.376767371894708
1102	68-69	0.38342210257259524
1102	70-71	0.3174833990295909
1102	72-73	0.3161590379575685
1102	74-75	0.3425308109207137
1102	76-77	0.31133261791635647
1102	78-79	0.3479391245182626
1102	80-81	0.29745008530349537
1102	82-83	0.3189888813980275
1102	84-85	0.32794487933892924
1102	86-87	0.3883704638850958
1102	88-89	0.3477791012703477
1102	90-91	0.2920213148351962
1102	92-93	0.29598988464481124
1102	94-95	0.2965045084245297
1102	96-97	0.2766890430912845
1102	98-99	0.26239990562786275
1102	100	0.25629548841218863
1103	1	0.1301129047757037
1103	2	0.2073808525354437
1103	3	0.2676519236793453
1103	4	0.20332022048835086
1103	5	0.20358940973184048
1103	6	0.15626825752415385
1103	7	0.20984739890762683
1103	8	0.1859054667235256
1103	9	0.2823136543202196
1103	10-11	0.2465859085221851
1103	12-13	0.2844385074170148
1103	14-15	0.3398596983994935
1103	16-17	0.3411771637722012
1103	18-19	0.36465513001823524
1103	20-21	0.34353184121084723
1103	22-23	0.3007226441171298
1103	24-25	0.28077591970917126
1103	26-27	0.3066652380203152
1103	28-29	0.2752689625109994
1103	30-31	0.29263556011422054
1103	32-33	0.2955784339620209
1103	34-35	0.22174081930964462
1103	36-37	0.2665919491030806
1103	38-39	0.3071392122948424
1103	40-41	0.32929002664200624
1103	42-43	0.302702659213665
1103	44-45	0.3261574632763029
1103	46-47	0.33753266992943765
1103	48-49	0.33150223243313093
1103	50-51	0.29312431957905716
1103	52-53	0.28528639501111996
1103	54-55	0.32121372226229283
1103	56-57	0.2587189075302945
1103	58-59	0.3240148106781575
1103	60-61	0.3441283538860773
1103	62-63	0.36725094275740844
1103	64-65	0.32595565608180266
1103	66-67	0.2935455437401373
1103	68-69	0.35915056457956496
1103	70-71	0.3310245631316775
1103	72-73	0.32035917328645525
1103	74-75	0.23808933766655827
1103	76-77	0.2976672027424243
1103	78-79	0.3286729372340389
1103	80-81	0.22248068827833833
1103	82-83	0.2876592837234213
1103	84-85	0.32565093936952394
1103	86-87	0.31390076574889747
1103	88-89	0.31814158505996915
1103	90-91	0.3281563066744013
1103	92-93	0.3274264751098528
1103	94-95	0.3182875391070574
1103	96-97	0.3337215896009589
1103	98-99	0.333290231922728
1103	100	0.2909287317543843
1104	1	0.11763125013133191
1104	2	0.17267927852855536
1104	3	0.16249068154691315
1104	4	0.15183387731281783
1104	5	0.10966184556824032
1104	6	0.10182170448103989
1104	7	0.10865786470728978
1104	8	0.15473350630681182
1104	9	0.19987824474681304
1104	10-11	0.14997670071321778
1104	12-13	0.1994478811960434
1104	14-15	0.25750193851926184
1104	16-17	0.2339815571360262
1104	18-19	0.229459360038625
1104	20-21	0.1967002318758091
1104	22-23	0.24882701893039183
1104	24-25	0.2460481615034169
1104	26-27	0.2461953547739384
1104	28-29	0.2655719440622235
1104	30-31	0.25115991223998435
1104	32-33	0.28782399167725714
1104	34-35	0.21860831905519262
1104	36-37	0.29915271860661363
1104	38-39	0.22343943014081447
1104	40-41	0.24526524030178365
1104	42-43	0.234729115104372
1104	44-45	0.14881249999984192
1104	46-47	0.20855899202627626
1104	48-49	0.22711215189415412
1104	50-51	0.254383894459302
1104	52-53	0.1963087730617019
1104	54-55	0.1934925355517194
1104	56-57	0.1390329045031038
1104	58-59	0.11786577169571189
1104	60-61	0.1401747655837653
1104	62-63	0.17666401860458336
1104	64-65	0.1410981670725988
1104	66-67	0.16766845516038842
1104	68-69	0.12819532150155766
1104	70-71	0.08498787816617437
1104	72-73	0.07334004732978627
1104	74-75	0.1061566811277288
1104	76-77	0.1376392907147661
1104	78-79	0.042455634649769536
1104	80-81	0.10888647194924772
1104	82-83	0.024889582371038443
1104	84-85	0.03942347912189348
1104	86-87	0.029641840138296516
1104	88-89	0.00131657512802974
1104	90-91	-0.0113541308890035
1104	92-93	-0.03397333708623762
1104	94-95	-0.008938076755146795
1104	96-97	0.024172380544349892
1104	98-99	0.11107197472330554
1104	100	0.056076003508611194
1105	1	0.1144041200634689
1105	2	0.11123594792418956
1105	3	0.1195908557088714
1105	4	0.08161408812647863
1105	5	0.0633919214756773
1105	6	0.17701570903088282
1105	7	0.10856596216458314
1105	8	0.08472828652348596
1105	9	0.1431323677705123
1105	10-11	0.1618191475852413
1105	12-13	0.12862410803057145
1105	14-15	0.1764110038032527
1105	16-17	0.23728212334782484
1105	18-19	0.20001347693287386
1105	20-21	0.17888339746943416
1105	22-23	0.2008870179387472
1105	24-25	0.16377730979640148
1105	26-27	0.19789670352378153
1105	28-29	0.2723082364700318
1105	30-31	0.24582437230863974
1105	32-33	0.2886281974874123
1105	34-35	0.33589813883797603
1105	36-37	0.3287458253693458
1105	38-39	0.28599730356125974
1105	40-41	0.28255653193073016
1105	42-43	0.29710056769159365
1105	44-45	0.2671494582911649
1105	46-47	0.24676796216731844
1105	48-49	0.22832740100118798
1105	50-51	0.3202953322656015
1105	52-53	0.32808889876595515
1105	54-55	0.3166863519238774
1105	56-57	0.3368010664795804
1105	58-59	0.3378747394162147
1105	60-61	0.3548158478943293
1105	62-63	0.3679657591700334
1105	64-65	0.37590931984047415
1105	66-67	0.39680146359441437
1105	68-69	0.3788983915696349
1105	70-71	0.36947305800273966
1105	72-73	0.33844448789354686
1105	74-75	0.3626818983031157
1105	76-77	0.3611005184999385
1105	78-79	0.36016520387514817
1105	80-81	0.3174418217586634
1105	82-83	0.2745155694742394
1105	84-85	0.25120437783818517
1105	86-87	0.18044460143100594
1105	88-89	0.25396449273900146
1105	90-91	0.2309536575196418
1105	92-93	0.22586302641525435
1105	94-95	0.21792204203103793
1105	96-97	0.24288717775167257
1105	98-99	0.2066347099210013
1105	100	0.2061749529540151
1106	1	0.09438800969059358
1106	2	0.11865998901124541
1106	3	0.1150840630000296
1106	4	0.1267119184592076
1106	5	0.015465618021295313
1106	6	0.06852561728398854
1106	7	0.07852067743897351
1106	8	0.07370370792560976
1106	9	0.08346418164524039
1106	10-11	0.13720457237017314
1106	12-13	0.18848657179624695
1106	14-15	0.2401072503854067
1106	16-17	0.13578976456122405
1106	18-19	0.18798405587838118
1106	20-21	0.20871375262652236
1106	22-23	0.181414866269499
1106	24-25	0.19497499079980685
1106	26-27	0.24904171743813208
1106	28-29	0.17916299454343942
1106	30-31	0.18222914262369017
1106	32-33	0.2306949602182513
1106	34-35	0.18602849829365908
1106	36-37	0.24785334435322426
1106	38-39	0.18520561516801592
1106	40-41	0.19784316387225687
1106	42-43	0.21687334041385498
1106	44-45	0.17845989321515532
1106	46-47	0.19407825878881368
1106	48-49	0.2770565131177918
1106	50-51	0.1817626657746203
1106	52-53	0.26565129842654756
1106	54-55	0.27773298416443737
1106	56-57	0.22596385039686595
1106	58-59	0.1800025234549878
1106	60-61	0.3303178018844477
1106	62-63	0.22397266681946348
1106	64-65	0.24662899748349787
1106	66-67	0.26803295395342275
1106	68-69	0.24764538956424786
1106	70-71	0.289926316428442
1106	72-73	0.3160891962341239
1106	74-75	0.24934037895665284
1106	76-77	0.2998308267463159
1106	78-79	0.2473141911830865
1106	80-81	0.20756636877123924
1106	82-83	0.2416779758630625
1106	84-85	0.24114268370725256
1106	86-87	0.2190312806570347
1106	88-89	0.21143282800669994
1106	90-91	0.18356178110975208
1106	92-93	0.17608851556691718
1106	94-95	0.17091217956727078
1106	96-97	0.21503819287336867
1106	98-99	0.19148862322775884
1106	100	0.20235070179666437
1107	1	0.012473996280697008
1107	2	0.07066262518766919
1107	3	0.041387891665792154
1107	4	0.050654674950664
1107	5	0.01178674480005526
1107	6	0.048565303512944524
1107	7	0.06672116755815694
1107	8	0.04587402703433696
1107	9	0.0626580946047568
1107	10-11	0.01077661655197204
1107	12-13	0.047421303815490035
1107	14-15	0.14804598584632345
1107	16-17	0.07065490281510733
1107	18-19	0.0616273529224074
1107	20-21	0.0692325596131127
1107	22-23	0.06890952230482839
1107	24-25	0.059010494467813146
1107	26-27	0.06614037626901847
1107	28-29	0.11618689709680297
1107	30-31	0.1121156621205941
1107	32-33	0.052254227702931644
1107	34-35	0.0759120635786914
1107	36-37	0.08888278847680198
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2216	52-53	-0.3258679725065363
2216	54-55	-0.3125340022263572
2216	56-57	-0.3015174772251257
2216	58-59	-0.26473217564193163
2216	60-61	-0.33072800827284254
2216	62-63	-0.32077349245213327
2216	64-65	-0.3168820346829122
2216	66-67	-0.34552925652731403
2216	68-69	-0.29400449596245437
2216	70-71	-0.3371132450079415
2216	72-73	-0.34871653596542274
2216	74-75	-0.32257408675230437
2216	76-77	-0.3571586809856626
2216	78-79	-0.2599399281288939
2216	80-81	-0.30616424776331996
2216	82-83	-0.30472791069919225
2216	84-85	-0.3098906872367593
2216	86-87	-0.2762529502282405
2216	88-89	-0.20690810405515236
2216	90-91	-0.2504843284677847
2216	92-93	-0.2796158969856677
2216	94-95	-0.34694392859166
2216	96-97	-0.3688751470839371
2216	98-99	-0.3475167449292833
2216	100	-0.33914706168530984
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	44879.0
3	6504.0
4	4145.0
5	3689.0
6	2918.0
7	3395.0
8	4055.0
9	4393.0
10	4616.0
11	5167.0
12	5627.0
13	6082.0
14	6844.0
15	6902.0
16	6783.0
17	6639.0
18	6590.0
19	6219.0
20	6412.0
21	6832.0
22	8093.0
23	11822.0
24	18425.0
25	25120.0
26	25931.0
27	21852.0
28	22986.0
29	28931.0
30	35604.0
31	42649.0
32	55819.0
33	75124.0
34	109062.0
35	172317.0
36	329432.0
37	697385.0
38	1156874.0
39	535266.0
40	1030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47996679268098	19.51387417017217	13.209995380295968	23.796163656850887
2	15.361835254527575	19.91201270371994	40.71884325458667	24.007308787165808
3	18.30971909248806	25.469370150845688	29.735816183684484	26.485094572981772
4	12.322328206914598	16.563408070678605	38.8915297454369	32.2227339769699
5	12.859364735886238	38.409521233432514	35.46763444355292	13.263479587128332
6	31.106767747885666	38.07823509054861	17.7801057295458	13.034891432019927
7	27.713373106950645	32.69046416254639	22.194599023862466	17.401563706640495
8	25.293848063596023	36.917755500568106	19.95981919490077	17.828577240935097
9	25.22550883680968	17.249820718059684	20.816110712706802	36.708559732423836
10-11	24.01887824675855	27.214690051288404	28.554783041797428	20.211648660155618
12-13	24.558841453972878	24.46731254473097	28.697033485971485	22.276812515324664
14-15	21.834383586922257	26.487684836131887	25.679506424614218	25.998425152331638
16-17	21.67879085850756	29.899153449240124	26.220216841889638	22.201838850362673
18-19	21.585665345939432	28.89848450669332	28.121972465308893	21.393877682058356
20-21	22.732669486722074	28.14712756929464	27.62293421565895	21.49726872832434
22-23	22.434188292561384	27.804431574732018	27.691411204784927	22.06996892792167
24-25	22.192210262896808	27.909048136947725	27.734616053377152	22.164125546778312
26-27	22.007166452682632	27.923126555338147	27.77869798646659	22.291009005512635
28-29	22.012345349314234	28.083297441835885	27.855163886827572	22.049193322022305
30-31	22.19566367752819	28.384849507236083	27.436441445440824	21.983045369794905
32-33	21.937027788676616	28.145989636175138	27.86013069704237	22.056851878105874
34-35	22.07591508762968	27.86099256486196	27.90679009460798	22.15630225290038
36-37	22.29931159347722	28.201598862253498	27.450464006422838	22.04862553784645
38-39	22.140500941648476	28.36502607609156	27.60139447499937	21.893078507260594
40-41	21.997802427273765	28.024492921102595	27.84198216230076	22.13572248932288
42-43	21.893139838877424	28.022071142085426	27.802018883580704	22.282770135456442
44-45	22.014378963889502	28.113533807669427	27.787742649277504	22.084344579163574
46-47	22.032276097801446	28.216660001378045	27.681623195488537	22.06944070533197
48-49	21.9439114779089	28.0948243957154	27.727469367546114	22.233794758829582
50-51	21.913077378148127	28.04805141001911	27.76872140422821	22.27014980760455
52-53	22.03125213109835	27.99494617129712	27.79054542478757	22.18325627281696
54-55	22.044848765378163	28.316663192980794	27.604706766214406	22.033781275426634
56-57	21.963351272544596	28.040150759273175	27.682076885297164	22.314421082885065
58-59	21.89537576253611	27.71968193086169	27.997198973070446	22.387743333531755
60-61	22.114451078918993	28.109018481344624	27.61146130339722	22.165069136339163
62-63	21.859957840398717	28.137314486246982	27.67699119444532	22.325736478908976
64-65	21.909812665550508	27.860091220149407	27.79671587572977	22.43338023857031
66-67	21.807502963109133	27.884380568482236	27.71472720998645	22.593389258422185
68-69	22.132172498825838	27.99500332003897	27.663766569295905	22.209057611839285
70-71	22.050241361969082	27.784541469083223	27.768062370546737	22.39715479840096
72-73	21.790510712586567	27.89267255015916	27.918215069432893	22.39860166782138
74-75	21.88846682909789	28.070825923136816	27.638750913267213	22.40195633449808
76-77	21.946711012123686	27.930719599368004	27.57653554810593	22.546033840402384
78-79	21.997687824483776	27.973772235653243	27.69070262803267	22.33783731183031
80-81	21.91034819887069	27.792052667595467	27.82482643187752	22.472772701656318
82-83	21.794271311681165	27.807111117551237	27.934156750416207	22.464460820351395
84-85	21.820691371648415	27.821459631820407	27.78506387441219	22.572785122118987
86-87	21.951426367447482	27.894010833602067	27.72416222843188	22.430400570518565
88-89	21.72129366009048	27.760824088513885	27.763636813052408	22.754245438343233
90-91	21.73863324814869	27.647881061592898	27.866458511195937	22.747027179062478
92-93	21.885155034112675	27.767340018171456	27.752239754122815	22.595265193593058
94-95	21.67308606367235	27.59701471759341	27.844684969405943	22.8852142493283
96-97	21.729477045320696	27.49784293375782	27.954981878370727	22.817698142550753
98-99	21.767982343558252	27.613929974542938	27.7883693005537	22.829718381345106
100	21.80407594135472	27.384588198327553	27.760027773721447	23.051308086596283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3054.0
1	1841.0
2	588.0
3	485.0
4	377.5
5	298.5
6	270.5
7	294.5
8	325.5
9	330.5
10	326.0
11	323.5
12	318.5
13	358.5
14	403.0
15	474.0
16	590.0
17	731.5
18	1011.5
19	1381.5
20	1956.5
21	2859.5
22	4300.0
23	6599.5
24	9858.5
25	14414.5
26	20336.0
27	27261.5
28	36358.0
29	46949.0
30	56870.5
31	66513.5
32	75380.0
33	84170.5
34	93351.5
35	101084.5
36	107723.0
37	113834.0
38	119113.5
39	121803.0
40	123950.5
41	126246.5
42	126658.5
43	126223.5
44	123899.0
45	121344.5
46	120334.0
47	118373.0
48	116256.5
49	113992.0
50	110534.0
51	106485.5
52	100378.0
53	95498.0
54	92158.0
55	86667.5
56	79944.5
57	73129.0
58	66462.0
59	61725.5
60	55956.0
61	47879.5
62	41121.0
63	35238.0
64	29084.5
65	23477.5
66	18863.0
67	14662.0
68	11129.0
69	8439.0
70	6134.0
71	4426.5
72	3470.5
73	2635.5
74	1975.5
75	1389.0
76	915.5
77	672.5
78	477.5
79	314.5
80	223.5
81	150.0
82	106.5
83	79.5
84	72.0
85	50.5
86	41.5
87	40.5
88	31.0
89	26.0
90	19.0
91	12.5
92	10.0
93	9.5
94	9.0
95	6.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07685072704421655
2	0.07988841740023103
3	0.08108078183904045
4	0.08323839368069559
5	0.08144984702248147
6	0.08139306776349053
7	0.08025748258367205
8	0.08011553443619474
9	0.07997358628871742
10-11	0.07967549517901507
12-13	0.07835537740747607
14-15	0.07686492185896429
16-17	0.0755022196431821
18-19	0.07480667372054328
20-21	0.07449438779609319
22-23	0.07646746704602782
24-25	0.07679394778522564
26-27	0.07608420704783908
28-29	0.07225160706595166
30-31	0.07737593518988262
32-33	0.07580031075288446
34-35	0.07303232187707688
36-37	0.07019335892753065
38-39	0.07159864558755603
40-41	0.07439502409285907
42-43	0.07933481962506952
44-45	0.08025748258367205
46-47	0.0833377573839297
48-49	0.0870284092183398
50-51	0.0879794618064378
52-53	0.08803624106542872
54-55	0.08790848773269914
56-57	0.08594960329751225
58-59	0.08487079737668468
60-61	0.08284093886775912
62-63	0.08318161442170467
64-65	0.08248606849906584
66-67	0.08186149665016566
68-69	0.08164857442894971
70-71	0.07957613147578095
72-73	0.07924965073658313
74-75	0.07862507888768296
76-77	0.07875283222041254
78-79	0.07903672851536717
80-81	0.0803284566574107
82-83	0.07981744332649238
84-85	0.07869605296142161
86-87	0.07971807962325826
88-89	0.07648166186077555
90-91	0.0740401537241658
92-93	0.07401176409467032
94-95	0.07415371224214765
96-97	0.07341558187526562
98-99	0.07280520484111319
100	0.07208126928897889
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3522413.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.7578069157341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60991018832853	43.251568899303365
2	14.987564722292962	17.612728681791157
3	5.077996093434932	8.951157419307055
4	2.270183454053416	5.335640042262598
5	1.1913886628619952	3.5001692507019873
6	0.717747257137906	2.5303952849524096
7	0.4574530234171574	1.8815255496074885
8	0.3081160674619303	1.4483379519650725
9	0.2269078288631148	1.1999345756406603
>10	1.0905207396328906	11.037023198047448
>50	0.0498151950006625	1.9596606308244664
>100	0.012203361106870548	1.1566537032987232
>500	1.4505480573952584E-4	0.06778707336906922
>1k	4.835160190587196E-5	0.06741773892845003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	1.4194814747731172E-5	0.0	0.0	0.0
16-17	0.0	2.8389629495462344E-5	0.0	0.0	0.0
18-19	0.0	2.8389629495462344E-5	0.0	0.0	0.0
20-21	0.0	2.8389629495462344E-5	0.0	0.0	0.0
22-23	0.0	4.258444424319351E-5	0.0	1.4194814747731172E-5	0.0
24-25	0.0	8.516888848638702E-5	0.0	5.677925899092469E-5	0.0
26-27	0.0	1.1355851798184937E-4	0.0	1.987274064682364E-4	0.0
28-29	0.0	1.5614296222504287E-4	0.0	9.794422175934507E-4	0.0
30-31	0.0	1.7033777697277405E-4	0.0	0.00411649627684204	0.0
32-33	0.0	1.7033777697277405E-4	0.0	0.011171319206464431	0.0
34-35	0.0	1.7033777697277405E-4	0.0	0.022271664339190207	0.0
36-37	0.0	1.987274064682364E-4	0.0	0.043166431647850494	0.0
38-39	0.0	2.4131185071142992E-4	0.0	0.08078269072933811	0.0
40-41	0.0	2.8389629495462345E-4	0.0	0.1258654223681323	0.0
42-43	0.0	2.8389629495462345E-4	0.0	0.17205534955724955	0.0
44-45	0.0	2.8389629495462345E-4	0.0	0.22637890559681673	0.0
46-47	0.0	2.8389629495462345E-4	0.0	0.29366232750106247	0.0
48-49	0.0	2.8389629495462345E-4	0.0	0.3699736515848653	0.0
50-51	0.0	2.8389629495462345E-4	0.0	0.45711561932118694	0.0
52-53	0.0	2.8389629495462345E-4	0.0	0.5452654189045976	0.0
54-55	0.0	2.8389629495462345E-4	0.0	0.6406829636388465	0.0
56-57	0.0	2.8389629495462345E-4	0.0	0.7571372238292329	0.0
58-59	0.0	2.8389629495462345E-4	0.0	0.8862816484040912	0.0
60-61	0.0	2.8389629495462345E-4	0.0	1.0255611707088295	0.0
62-63	0.0	2.8389629495462345E-4	0.0	1.1682758381825187	0.0
64-65	0.0	2.8389629495462345E-4	0.0	1.3220482663446904	0.0
66-67	0.0	2.8389629495462345E-4	0.0	1.498915658101421	0.0
68-69	0.0	2.8389629495462345E-4	0.0	1.697018492720757	0.0
70-71	0.0	2.980911097023546E-4	0.0	1.9125951442945504	0.0
72-73	0.0	3.26480739197817E-4	0.0	2.13807977656226	0.0
74-75	0.0	3.406755539455481E-4	0.0	2.367609931033073	0.0
76-77	0.0	3.406755539455481E-4	0.0	2.6104122372930147	0.0
78-79	0.0	3.406755539455481E-4	0.0	2.889723607084121	0.0
80-81	0.0	3.5487036869327927E-4	0.0	3.2056008196653827	0.0
82-83	0.0	3.6906518344101045E-4	0.0	3.522727175944445	0.0
84-85	0.0	3.6906518344101045E-4	0.0	3.8343033596571443	0.0
86-87	0.0	3.6906518344101045E-4	0.0	4.1679098958583225	0.0
88	0.0	3.6906518344101045E-4	0.0	4.444112601219675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11250	0.0	29.036362	1
GTATCAA	5890	0.0	28.88705	1
TCAACGC	5700	0.0	28.613077	4
ATCAACG	5810	0.0	28.233145	3
CAACGCA	5865	0.0	27.887848	5
TACATGG	11500	0.0	27.301628	2
GGTATCA	4040	0.0	27.22353	1
AACGCAG	6155	0.0	26.726603	6
ACATGGG	11730	0.0	25.76457	3
TATCAAC	6655	0.0	25.283949	2
CATGGGG	7955	0.0	23.338175	4
ACGCAGA	7485	0.0	21.53773	7
CGCAGAG	7665	0.0	20.786385	8
GAGTACA	7745	0.0	20.32981	1
GTATAGG	1480	0.0	20.007313	1
GTATAGA	1450	0.0	17.828083	1
TACACCG	640	0.0	17.62524	5
GCAGAGT	9030	0.0	17.435818	9
AGTACAT	8420	0.0	17.192877	2
GTATTAC	1405	0.0	17.060972	1
>>END_MODULE