FastQCFastQC Report
Wed 25 May 2016
SRR1032964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1032964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3522413
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77490.2199912389603377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.1776055221236124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62000.049.0258331
GTATCAA108100.037.6795581
TCAACGC125450.031.8738444
ATCAACG126400.031.5598533
CAACGCA127450.031.370995
AACGCAG131700.030.464926
TATCAAC134900.029.7800852
GTACATG110050.026.424881
ACGCAGA153850.025.7720157
CGCAGAG157850.025.089148
TACATGG110950.024.803252
ACATGGG112850.023.4272293
GCAGAGT173350.022.4637099
GAGTACT107800.021.11152312-13
CATGGGG79550.020.4016424
CAGAGTA165000.020.0985810-11
AGAGTAC151450.020.0513610-11
GTACTTT117550.019.24058314-15
AGTACTT111900.018.65890912-13
GAGTACA82800.018.3834511
Produced by FastQC (version 0.11.5)